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研究生:謝宇澤
研究生(外文):Yu-je Hsieh
論文名稱:具雙光束表面電漿子感測儀之研製與生醫應用
論文名稱(外文):Surface Plasmon Resonance Instrument with Dual Laser Beams and Its Bio-applications
指導教授:陳顯禎
指導教授(外文):Shean-jen Chen
學位類別:碩士
校院名稱:國立成功大學
系所名稱:工程科學系碩博士班
學門:工程學門
學類:綜合工程學類
論文種類:學術論文
論文出版年:2008
畢業學年度:96
語文別:中文
論文頁數:55
中文關鍵詞:動力學分析生物分子間作用雙光束表面電漿子
外文關鍵詞:biomolecular interaction analysissurface plasmon resonancekinetics studydual laser beams
相關次數:
  • 被引用被引用:1
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  • 下載下載:33
  • 收藏至我的研究室書目清單書目收藏:0
  表面電漿子共振(surface plasmon resonance,SPR)之共振條件極易因微小濃度的生物分子破壞而改變,因此可利用此高靈敏度偵測訊號改變之特性來定量分析生物分子。除了具備高靈敏度外,另具有即時(real-time)監測、不需標定待測物(label free)、可動力學分析(kinetics study)、及大量平行篩檢(high-throughput screening)等優點,於生物分子間作用的分析研究上,已佔有重要的一席之地。

  然而傳統SPR感測器,對於背景訊號擾動如環境溫度變動、緩衝溶液濃度變化等,存在著干擾量測訊號的問題,進而影響量測實驗數據的準確度及可信度,因此本研究是將單一波長雷射分為兩道平行入射光束,利用衰逝全反射(attenuated-total-reflection,ATR)的方式,同時在感測晶片上之金屬薄膜激發表面電漿子,並將感測晶片的金屬薄膜自中間劃分為左右兩區域,以不同表面改植來做修飾處理,可得到同時具有官能基受器分子修飾之感測訊號區域及無任何修飾過之參考訊號區域的感測晶片。在沿用原有ATR的方式之下,具雙光束之表面電漿子感測系統除了保有原先量測優點,更具有消除背景訊號干擾的優勢,因而在微量生物分子之量測及動態分析時,能將系統探測能力提升。
  The resonance condition of surface plasmon resonance (SPR) is easily destroyed by environmental disturbances on the interface between metal and dielectric medium. Therefore, with the helps of high sensitivity about the disturbances, biomolecular interaction on the interface can be quantitatively determined. Additionally, there are some other advantages such as real-time analysis, label free, kinetics study, and high-throughput screening, the SPR biosensors play a significant role in biomolecular interaction analysis (BIA).

  Conventional SPR biosensors, however, detect the overall signal on the sensing surface according to specific biomolecular interactions, nonspecific interactions (e.g. biomolecules adsorption on the sensing surface), and background interferences (e.g. the refractive index change and fluctuation of buffer solution, temperature fluctuation), so interested specific interaction signal is difficult to be exactly extracted from the detection signal. Therefore, this study has developed a new SPR instrument with dual laser beams based on attenuated-total-reflection (ATR) method. Also, its sensor chip has two sensing regions corresponding to the dual laser beams: one with surface modified bio-receptors, and the other without any bio-coating. The dual-laser-beam SPR system not only retains original advantages of conventional SPR, but also discriminates the specific response from the nonspecific interaction and background interferences. Certainly, the SPR system will be beneficial for real-time BIA.
摘要 Ⅰ
Abstract Ⅲ
誌謝 Ⅴ
目錄 Ⅵ
圖目錄 Ⅷ
表目錄 Ⅹ
第一章 緒論 1
1.1 前言 1
1.2 研究動機與目的 2
1.3 文獻回顧 3
1.4 論文架構 9
第二章 表面電漿子 11
2.1 表面電漿子之色散關係 11
2.2 稜鏡式耦合激發 15
2.3 表面電漿子生醫感測儀 16
  2.3.1 光路之設計 16
  2.3.2 人機介面之設計 18
  2.3.3 旋轉平台之操控 20
  2.3.4 感測晶片之製作 20
2.4 生物分子之感測分析實驗 22
第三章 雙光束表面電漿子生醫感測系統 26
3.1 感測儀之研製 26
  3.1.1 光路設計 26
  3.1.2 提升儀器量測精準度 28
  3.1.3 人機介面程式之設計 29
3.2 感測儀之校正方式與靈敏度 31
  3.2.1 光路之校正 31
  3.2.2 模擬感測器之靈敏度 32
  3.2.3 量測氮氣與氬氣之折射係數差異 33
3.2.4 溶液切換之背景訊號量測 35
3.3 雙光束表面電漿子感測晶片之製作 38
第四章 蛋白質分子與感測晶片表面交互作用之實驗結果與討論 41
4.1 數據量測與分析 41
4.2 動力學分析 45
第五章 結論 50
參考文獻 52
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