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研究生:王炳順
研究生(外文):Bing-Shun Wang
論文名稱:以Polypyrrole修飾的碳電極感測半胱胺酸與高半胱胺酸的研究
論文名稱(外文):Study of Sensing Capability of Polypyrrole-Modified Carbon Electrodes on Cysteine and Homocysteine
指導教授:陳炳宏陳炳宏引用關係
指導教授(外文):Bing-Hung Chen
學位類別:碩士
校院名稱:國立成功大學
系所名稱:化學工程學系碩博士班
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:2008
畢業學年度:96
語文別:中文
論文頁數:60
中文關鍵詞:高分子拓印模板修飾電極聚吡咯高半胱胺酸半胱胺酸
外文關鍵詞:homocysteinecysteinepolypyrrole modified carbon electrodemolecularly imprinted polymer
相關次數:
  • 被引用被引用:2
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  • 評分評分:
  • 下載下載:16
  • 收藏至我的研究室書目清單書目收藏:0
  在本研究中,我們利用聚吡咯修飾的碳電極針對不同濃度的半胱胺酸進行感測。藉由改變聚合過程中半胱胺酸的摻雜量,觀察聚吡咯修飾的碳電極對半胱胺酸的感測靈敏度、拓印係數與吸附情形的變化。藉由比較0.2 mM的半胱胺酸溶液與背景溶液的循環伏安圖,決定半胱胺酸在聚吡咯修飾電極上的感測電位為0.7 V (vs. Ag/AgCl, 3 M KC1)。當摻雜0.5 mM的半胱胺酸時,聚吡咯修飾電極具有最大的靈敏度,數值為89.80 uA˙cm-2˙mM-1,感測濃度的範圍在0.025 ~ 0.2 mM間。以同樣的電極感測0.15 mM的半胱胺酸溶液時,也可以得到較高的拓印係數,數值為1.27。在相同的感測電位(0.7 V vs. Ag/AgCl, 3 M KC1)下,以同樣摻雜0.5 mM的半胱胺酸所製備的聚吡咯修飾電極,分別感測半胱胺酸與高半胱胺酸溶液,可以得到聚吡咯修飾電極對半胱胺酸的選擇性為1.11。另外,以Langmuir恆溫吸附模式來比較不同摻雜量的聚吡咯修飾電極對半胱胺酸的吸附情形,我們發現當摻雜0.5 mM的半胱胺酸時,聚吡咯修飾電極具有最大的飽和吸附量。
In this study, successful fabrication of the screen-printed carbon electrode electro-deposited with polypyrrole film, abbreviated as PPy-C modified electrode, to detect cysteine with a concentration range from 0.025 mM to 0.2 mM in solution was achieved. Varying amount of cysteine present initially and conjointly with pyrrole during electropolymerization significantly changes the sensitivity, imprinting factor and adsorption capacity of the PPy-C modified electrode. With cyclic voltammogram obtained from 0.2 mM cysteine solution, the sensing potential of cysteine at the PPy-C modified electrode was determined at 0.7 V (vs. Ag/AgCl, 3 M KC1). The PPy-C modified electrode doped with cysteine (0.5 mM initially present) gave a maximum sensitivity as 89.80 uA˙cm-2˙mM-1 and an imprinting factor as high as 1.27 when 0.15 mM cysteine in solution was sensed. Furthermore, the same PPy-C modified electrode at the same sensing potential could be used in detecting mixtures of cysteine and homocysteine, from which the selectivity of cysteine was 1.11. Langmuir isotherm was employed successfully in accounting for the adsorption behavior of cysteine on the PPy-C electrodes. Likewise, the PPy-C modified electrode made from 0.5 mM cysteine initially present in electropolymerization of polypyrrole rendered the maximum saturated adsorption capacity.
摘要 I
Abstract II
誌謝 III
目錄 IV
表目錄 VI
圖目錄 VII
第一章 緒論 1
1.1 前言 1
1.2 半胱胺酸與高半胱胺酸 1
1.3 聚合物修飾電極 4
1.4 半胱胺酸與高半胱胺酸的感測概況 6
1.5 拋棄式網版印刷電極 7
1.6 研究動機與目的 7
第二章 原理 8
2.1 電極表面的電化學反應過程 8
2.2 半胱胺酸在電極上的電化學反應 9
2.2.1 汞電極 9
2.2.2 金與白金電極 10
2.2.3 碳電極與石墨電極 12
2.3 吡咯單體的聚合反應機制 13
2.4 分子拓印 14
2.5 以聚吡咯修飾的碳電極感測半胱胺酸與高半胱胺酸 16
第三章 實驗設備與方法 17
3.1 藥品與材料 17
3.2 儀器設備與測量原理 17
3.3 電化學系統 21
3.4 化學修飾電極的製備 22
3.4.1 網版印刷碳電極 (Screen-Printed Carbon Electrode) 22
3.4.2 聚吡咯修飾網印碳電極(Polypyrrole-modified Carbon Electrode) 22
3.5 胺基酸的感測性質與分析 23
3.5.1 溶液的配製 23
3.5.2 感測方法 23
3.5.3 半胱胺酸的感測電位 25
3.5.4 靈敏度 25
3.5.5 半胱胺酸的拓印係數 26
3.5.6 半胱胺酸吸附情形的探討 26
3.6 電極的表面型態 27
3.7 聚吡咯修飾電極的辨識選擇性 27
第四章 結果與討論 28
4.1 修飾電極的製備 28
4.2 半胱胺酸感測的性質分析 30
4.2.1 半胱胺酸的感測電位 30
4.2.2 半胱胺酸的靈敏度 32
4.2.3 半胱胺酸的拓印係數 38
4.2.4 半胱胺酸的吸附情況 41
4.3 聚合前後電極表面的變化 44
4.4 聚吡咯修飾電極的辨識選擇性 50
第五章 結論與建議事項 52
參考文獻 53
自述 60
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