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研究生:游秉鈞
研究生(外文):Ping-chun Yu
論文名稱:互補式金氧半導體光二極體晶片葡萄糖與組織胺濃度檢測光學感測系統
論文名稱(外文):An optical bio-sensing system for the determination of glucose and histamine based on CMOS photodiode chip
指導教授:楊裕雄
指導教授(外文):Yuh-Shyong Yang
學位類別:碩士
校院名稱:國立交通大學
系所名稱:理學院碩士在職專班應用科技學程
學門:環境保護學門
學類:環境防災學類
論文種類:學術論文
論文出版年:2008
畢業學年度:96
語文別:英文
論文頁數:62
中文關鍵詞:互補式金氧半導體光二極體感應晶片耦合酵素催化反應葡萄糖組織胺
外文關鍵詞:CMOS photodiode chipcoupled enzyme reactionsglucosehistamine
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在此研究中,我們經由整合互補式金氧半導體製程所生產的光二極體感應晶片、耦合酵素催化反應,發展出一套生醫感測系統。由於生醫診斷上的重要性,我們選擇了葡萄糖與組織胺做為本系統的測量標的。我們發展的組裝系統所量測得到的葡萄糖與組織胺的Km值分別為4.9 and 125 uM,其值與UV/Vis光譜儀所分別測量而得到的值(7.2 and 160 uM)相當接近。我們的系統對於葡萄糖與組織胺的最低量測濃度分別為1 uM 與 10 uM。 再者,我們的系統能測量到葡萄糖與組織胺的濃度線性量測範圍,分別為1 uM 至 5 mM 以及10 至 80 uM。兩者的濃度線性量測範圍都小於人體血液中葡萄糖與組織胺正常含量的2到10倍。經由樣本的稀釋,我們的系統將能夠測量人體血液中的葡萄糖與組織胺的濃度。未來,我們的目標將特別針對多功能家用醫療診斷儀器進行進一步的研究。
We developed a biomedical diagnosis system by integrating CMOS photodiode chip and coupled enzyme reactions. Glucose and histamine are the chosen targets because of their importance in the medical diagnosis. The Km value of glucose and histamine obtained from our assembled system is 4.9 and 125 uM, respectively, which are similar to those obtained from UV/Vis spectrophotometer (7.2 and 160 uM). The detection limits of glucose and histamine concentrations in our system are 1 uM and 10 uM, respectively. The linear ranges are from 1 uM to 5 mM and 10 to 80 uM for glucose and histamine, respectively, which both are 2 to 10 folds lower than those in the human blood. In the future, our research will particularly aim at multi-functional household medical diagnosis instrument.
CONTENTS PAGE
Abstract (Chinese) …………………………………………i
Abstract (English) …………………………………………ii
Acknowledgement………………………………………………iii
Contents …………………………………………………….iv
Contents of TABLES and FIGURES ………………………..vi
Abbreviation ………………………………………………..viii

Chapter 1. Introduction
1.1 Development progress of optical biosensor …1
1.2 The design of CMOS photodiode chip …………3
1.3 Glucose and histamine determination in medical
diagnosis ………………………………………….4
1.4 Biochemical reactions ………………………….5
Chapter 2. Materials and methods
2.1 Materials ………………………………………….8
2.2 Equipments ………………………………………..8
2.3 Methods …………………………………………...9
Chapter 3. Results
3.1 CMOS photodiode chip performance …………..19
3.2 Optimization of enzyme assay ………………..20
3.3 The determination of hydrogen dioxide ……..24
3.4 Coupled enzyme assay to determine β-D(+)-glucose
and histamine …………………………………….24
Chapter 4. Discussion …………………………………26
Chapter 5. Conclusion ………………………………..28
Reference …………………………………………………29
Tables and figures ………………………………….. 33
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