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研究生:謝佳玲
研究生(外文):Chia-lin Shieh
論文名稱:山葵過氧化酶之生化研究
論文名稱(外文):The biochemical studies of peroxidase in Wasabia japonica
指導教授:劉景煌劉景煌引用關係
指導教授(外文):Zin -huang Lin
學位類別:碩士
校院名稱:國立中山大學
系所名稱:生物科學系研究所
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2008
畢業學年度:96
語文別:英文
論文頁數:34
中文關鍵詞:過氧化山葵
外文關鍵詞:LC-MS/MSWasabia japonicaperoxidase
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植物過氧化酶 (EC 1.11.17) 是屬於同功異構酶的家族。 這些同功異構酶的胺基酸序列有50% 以上的不同。 過氧化酶可作為IAA (植物生長素) 氧化酶。 以純化後的山葵 (Wasabia japonica) 過氧化酶進行動力分析,以IAA為受質的情況下,其對受質的親和力為 17.1 μM。針對以syringalazine (木質素單體的類似物) 為受質的情況下,親和力為80.7 μM 。以二維電泳純化後的山葵蛋白質,用LC/MS/MS (液相層析質譜儀) 做蛋白質定序,其胺基酸序列跟蛋白質資料庫比對,比對結果與阿拉伯介 (Arabidopsis thaliana)
果有10% 相似。
The plant peroxidases (EC1.11.17) exit as a large family of isozymes. These isozymes have more than 50% amino acid sequence differences. The function of Wasaba japonica peroxides plays the role as IAA oxidases. The kinetics result shows Wasabia japonica peroxidases displayed affinity (Km = 17.1 μM) for IAA. The kinetics results in Wasabia japonica peroxidases display affinity (Km = 80.6 μM) for syringaldazine. LC/MS/MS technique described the data that has proven to be a method for identification and characterization of proteins. The soluble proteins extracted form Wasabia japonica was purified by gel filtration chromatography and two-dimensional gel electrophoresis (2-DE). LC-MS/MS analyses of 2-DE gel spots and identify proteins structure based on the protein fragmentation characteristics. The Mascot Search Results showed that Wasabia japonica peroxidase has a significant similarity (10%) with Arabidopsis thaliana peroxidase.
Chinese abstract ----------------------------------- I

English Abstract ---------------------------------- II

Table of contents ------------------------------- III

List of figures ----------------------------------- IV

Introduction ------------------------------------- 1

Materials and Methods ------------------------ 5

Results ------------------------------------------- 10

Discussion --------------------------------------- 12

References --------------------------------------- 15

Figures ------------------------------------------- 19
Reference
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Mansouri, I.E., Mercado, J.A., Santiago-D
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