本研究以釩(Ш)還原-化學發光法( V(Ш) reduction- chemilumine- scence method )進行海水低濃度Nitrite + Nitrate (N+N)的分析，其偵測極限以5 ml水樣為準約為0.7nM，另外以高溫催化氧化及螢光偵測法(HTCO- chemilu- minescence )進行海水總溶解有機氮(TDN)的分析，若以150 μL量測體積而言，其偵測極限為0.5μM，兩種方法均具高精確度與準確度。本研究並依據Magic (濃縮10倍)及 persulfate oxidation方法分別分析soluble reactive phosphorus (SRP)及Total dissolved phosphorus (TDP)，其偵測極限分別為10 nM (原水)及100 nM。這些分析方法均符合貧營養鹽海域探測的需求。
台灣東部黑潮海域(22~26°N)由水文資料分析可歸納為四種不同特性的水團，分別為1.受東海陸棚水影響之黑潮水( ECS-KW )，2.典型黑潮水團( KW )，3.受南海水影響之黑潮水( SCS-KW )，及4.受淡水影響之黑潮水團( plume-KW )。
在透光層範圍中，plume-KW的N+N濃度較其他水團高出約0.6 ~ 4μM，SRP也較其他水團高出約0.03~0.4μM， DON濃度高出約2~6μM，而DOP約0.05~0.2μM，顯示此水團受淡水輸入的影響。而SCS-KW與ECS-KW的氮磷物種大致亦較KW為高，透光層中Chl a與DON及DOP呈現顯著正相關，顯示生物作用控制透光層中DON及DOP的分布。
KW水團具貧營養鹽特性，在透光層中N+N與SRP均比其他水團低，Chl a除了與DON呈現出顯著正相關外，亦與N+N呈顯著正相關，乃因Chl a具次表層最高值特性所致。其N+N/SRP比值由透光層約0.02~0.15增加至深水層近似於 Redfield ratio。由nitrate anomaly 值 (2.5~ 1.5μM)分布顯示KW應有顯著的固氮作用。
台灣東南部SCS-KW水團在透光層中，春季N+N與SRP均較夏季高約0.02μM，DON高約1~2.5μM，而DOP則高出0.02~0.2μM。此與夏季水團層化較高具有密切關係。

This study used Vanadium (III) reduction- chemiluminescence method to measure the very low concentration of nitrite plus nitrate (N+N) in oligotrophic seawaters with a detection limit of 0.7nM. In addition, the high temperature catalytic oxidation (HTCO) – chemiluminescence method was applied to determine the concentration of total dissolved nitrogen (TDN) with a detection limit of 0.5μM based on a volume of 150μL seawater. Both methods have high degree of precision and accuracy. Furthermore, the Magic method and persulfate oxidation method were conducted to measure the concentrations of soluble reactive phosphorus (SRP) and total dissolved phosphorus (TDP) with detection limits about 10 nM (original seawater) and 100 nM, respectively. All these analytical methods meet the ranges of parameters in most oligotrophic oceans.
According to the hydrology data, the Kuroshio of east Taiwan may be classified into four types: 1. Kuroshio Waters affected by the East China Seawater (ECS-KW)；2. typical Kuroshio Waters (KW)；3. Kuroshio Waters affected by the South China Seawater (SCS- KW) and 4.Kuroshio waters affected by riverplume (plume- KW). In the euphotic zone, the concentrations of N+N, SRP, DON and DOP in the plume-KW are about 0.6 ~ 4 μM, 0.03 ~ 0.4 μM, 2 ~ 6 μM, and 0.05 ~ 0.2 μM, respectively, higher than those in other type waters. The plume-KW is apparently influenced by the input of freshwater. Besides, the nitrogen and phosphorus species in the SCS-KW and ECS-KW are higher than those in the KW. Positive correlations are significant between Ch1 a and DON and DOP, indicating that biological activity controls primarily on the distributions of DON and DOP in the euphotic zone.
KW has oligotrophic characteristics, and in the euphotic zone, the concentrations of N+N and SRP are generally lower than those in other water types. The Chl a has positive correlations with DON and N+N, resulted likely from their same features of subsurface maxima. The ratio between N+N and SRP in the euphotic zone is about 0.02~0.15, and the value increases gradually in deep water, and eventually close the Redfield ratio. Judging from the distribution of the nitrate anomaly (2.5~ 1.5μM), nitrogen fixation may prevail in the KW.
The N+N and SRP values in the euphotic zone in the SCS-KW near the southeastern coast of Taiwan are about 0.02μM higher in spring than in summer. DON and DOP are also about 1~2.5μM and 0.02~0.2μM higher, respectively, in spring than in summer. This feature may be caused from getting stratification torward the summer season in the SCS-KW.

致謝 ---------------------------------------------------Ⅰ
中文摘要 -----------------------------------------------Ⅱ
英文摘要 -----------------------------------------------Ⅳ
目錄 ---------------------------------------------------Ⅵ
圖目錄 -------------------------------------------------IX
表目錄 ------------------------------------------------XIV
第一章、 序論 --------------------------------------------1
第二章、 研究材料與方法 ----------------------------------3
2.1、研究材料 ----------------------------------------3
2.2、採樣方法 ----------------------------------------3
2.3、研究方法 ----------------------------------------7
2.3.1、低濃度溶解態無機氮之分析 --------------------7
2.3.2、溶解態有機氮之分析 -------------------------14
2.3.3、低濃度溶解態反應性磷之分析 -----------------17
2.3.4、非透光層溶解態無機氮、可利用性磷之分析直接注入
(FIA)測定法----------------------------------20
2.3.5、溶解態有機磷之分析 -------------------------20
第三章、結果與討論 -------------------------------------24
3.1、研究區域的水文特徵 -----------------------------24
3.1.1、台灣東部黑潮 [水試一號航次NCOR-JHS 2006年5月
22日~ 6月3日] ----------------------------24
3.1.2、台灣東南端黑潮區水文特徵[海研三號航次ORШ-
CR1217( 2007年4月21日~4月24日)] --------32
3.1.3、台灣東南端黑潮區水文特徵[海研三號航次ORШ-
CR1234 ( 2007年7月7日~ 7月10日)] --------34
3.2、溶解態氮磷物種分析之QA/QC --------------------36
3.3、各航次溶解態氮物種的分布 -----------------------38
3.3.1、水試一號航次NCOR-JHS (2006年5月22日~
6月3日) -----------------------------------38
3.3.2、海研三號航次ORШ-CR1217( 2007年4月21日~
4月24日) ---------------------------------62
3.3.3、海研三號航次ORШ-CR1234 ( 2007年7月7日~
7月10日)] --------------------------------65
3.4、各航次溶解態磷物種的分布 -----------------------69
3.4.1、水試一號航次NCOR-JHS (2006年5月22日~
6月3日) -----------------------------------69


3.4.2、海研三號航次ORШ-CR1217( 2007年4月21日~
4月24日) ---------------------------------82
3.4.3、海研三號航次ORШ-CR1234 ( 2007年7月7日~
7月10日)] --------------------------------85
3.5不同水團氮物種與磷物種之分布 --------------------88
第四章、結論 -------------------------------------------90
參考文獻 -----------------------------------------------93

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