臺灣博碩士論文加值系統

本研究針對葡萄籽萃取物前花青素(Grape Seed Proanthocyanidin Extract, GSPE)進行耐力運動前後之補充，探討其對耐力運動與運動後恢復期氧化壓力、發炎情形、肌肉損傷運動表現、運動後與恢復期心跳率與疲勞相關生化值之影響。本研究以10位國立體育學院一般非體育科系的學生為受試者，實驗採用雙盲交叉設計，每位受試者必須在進行75%VO2max強度之運動，分別於運動前、後與運動之後七天內每天服用三次每次500mg的GSPE，於運動前、運動後第30, 60, 120分鐘,運動後第1,2,3,5,7天進行靜脈採血。測量運動持續時間、心跳率與血液中麩胱甘肽、氧化型麩胱甘肽、丙二醛、細胞介白素- 6、腫瘤壞死因子α、血氨、血糖及血乳酸濃度與肌酸肌酶、乳酸脫氫酶的活性和白血球的數量。研究結果顯示：運動後衰竭點之麩胱甘肽、氧化型麩胱甘肽、丙二醛、細胞介白素- 6、腫瘤壞死因子α、血糖、血乳酸等的濃度與肌酸肌酶、乳酸脫氫酶的活性和白血球的數量兩組間並未達顯著差異，僅GSPE組在運動後衰竭點的心跳率和血氨顯著低於PL組;另在運動後7天的恢復期中GSPE組也僅心跳率在運動後120分鐘顯著低於PL組，總結以上結果，本研究發現，單次運動前補充GSPE並無法幫助運動表現、降低氧化壓力、減少肌肉損傷、延緩疲勞與抗發炎的作用。

This study investigated the effects of Grape Seed Proanthocyanidin Extract (GSPE) supplement on oxidative stress, inflammatory situation, muscle damage, heart rate and relative biological marker responses at post-exercise point and during recovery phase after endurance exercise. In this randomized, crossover study, ten healthy not sports-related department students were randomly divided into two groups, each one took either a placebo or GSPE 500mg, then performed a single bout of exercise at an estimated speed corresponding to the 75﹪VO2max to run exhausting. Blood samples of each people were collected before exercise, and 0, 30, 60, 120 minutes, day1, day2, day3, day5 and day7 after exercise, respectively. The experiment was repeated two weeks later, but treatments were exchanged for two groups. The concentrations of GSH, GSSG, MDA, IL-6, TNF-α, NH3, glucose and lactate were examined. Besides these, activity of creatine kinase and LDH, the amount of WBC, exercising time and heart rate were also examined. No differences in the levels of GSH, GSSG, MDA, IL-6, TNF-α, glucose, lactate, activity of creatine kinase and LDH between the two groups were observed at post-exercise. However, the concentration of NH3 and heart rate were significantly lower in the GSPE group, compared to that in the PL group at post-exercise. During the recovery phase, only heart rate was lower in GSPE group compare to that in the PL group at 120 minutes recovery period. The results indicated that GSPE supplementation before exercise may not enhance exercise performance, reduce oxidative stress, alleviate muscle damage, anti-fatigue and anti-inflammation.

第一章 緒論
第一節 研究動機………………………………………………1
第二節 研究目的………………………………………………1

第二章 文獻探討
第一節 運動與肌肉損傷………………………………………3
第二節 運動與氧化壓力………………………………………7
第三節 運動疲勞產生的機制…………………………………9
第四節 葡萄籽萃取物前花青素與其抗氧化功效……………12

第三章 研究方法
第一節 研究對象………………………………………………17
第二節 飲食活動控制與前測…………………………………17
第三節 實驗設計………………………………………………19
第四節 血液分析………………………………………………22
第五節 統計分析………………………………………………26

第四章 結果
第一節 補充GSPE對衰竭運動誘發的體內氧化壓力變化之影響……………………………………………………28
第二節 補充GSPE對衰竭運動誘發的體內發炎程度與肌肉損傷之影響……………………………………………………32
第三節 補充GSPE對運動表現與疲勞變化之影響…37
第四節 補充GSPE對衰竭運動後與恢復期心跳率之差異…41

第五章 討論
第一節 補充GSPE對衰竭運動誘發的體內氧化壓力變化之影響……………………………………………………42
第二節 補充GSPE對衰竭運動誘發的體內發炎程度與肌肉損傷之影響…………………………………………44
第三節 補充GSPE對運動表現與疲勞變化之影響………51
第四節 補充GSPE對衰竭運動後與恢復期心跳率之差異54

第六章 結論與建議

參考文獻………………………………………………………………57

附錄……………………………………………………………………78
附錄一 人體試驗同意書…………………………………………78
附錄二 受試者須知與同意書…………………………………………79
附錄三 受試者原始資料……………………………………………80

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