臺灣博碩士論文加值系統

本研究選擇聚己內酯（Poly(ε-caprolactone), PCL）、聚麩胺酸（γ-Polyglutamic acid, γ-PGA）和硫酸軟骨素（Chondroitin sulfate, CS）做成複合支架。先合成γ-PGA接枝CS的共聚物，然後利用IR和NMR進行合成結構鑑定。之後利用微粒溶濾法製作不同比例的三維多孔性支架，讓PCL重量比例較高，維持支架的機械強度。最後植入軟骨細胞進行細胞培養，比較不同支架中細胞的生長情形。研究中測量得知共聚物含量佔支架30wt%時含水率約有740%，高於其他含量較低的支架。經過軟骨細胞培養的支架進行切片染色、GAGs assay及collagen assay後，實驗結果也顯示出共聚物含量30wt%的支架細胞生長情形優於其他的支架。各個實驗數據都顯示出共聚物的添加對細胞生長和細胞外間質的分泌造成一定程度的助益。

封面
摘要
謝誌
目錄
第一章 緒論
第二章 文獻回顧
2-1 組織工程
2-1-1 組織工程簡介
2-1-2 組織工程應用於軟骨
2-2 軟骨組織學
2-2-1 軟骨組織
2-2-2 軟骨細胞
2-2-3 細胞外間質
2-3 可降解高分子材料
2-3-1 天然高分子材料
2-3-2 人工合成高分子
2-4 基材材料與設計
2-4-1 聚己內酯（Poly(ε-caprolactone), PCL）
2-4-2 聚麩胺酸（γ-Polyglutamic acid, γ-PGA）
2-4-3 硫酸軟骨素（Chondroitin sulfate, CS）
2-4-4 基材成型方式
第三章 研究動機與目的
第四章 實驗藥品、設備及實驗步驟
4-1 實驗藥品
4-1-1 支架製備
4-1-2 細胞植入前支架處理
4-1-3 GAGs定量分析
4-1-4 MTT毒性測試
4-1-5 細胞實驗
4-1-6 collagen 定量分析
4-2 實驗流程與步驟
4-2-1 實驗流程圖
4-2-2 合成實驗步驟
4-2-3 多孔性支架製備
4-2-4 細胞實驗
4-3 實驗結果鑑定方法
第五章 實驗結果與討論
5-1 實驗產率與CS定量分析（GAGs assay）
5-2 紅外線光譜儀分析
5-3 1H-NMR圖譜分析
5-4 表面電位及酸鹼值
5-5 化學分析電子能譜儀
5-6 支架吸水率及孔隙度測量
5-7 掃描式電子顯微鏡（SEM）圖分析
5-8 支架降解性質分析
5-9 細胞毒性測試
5-10 機械性質分析
5-11 細胞外間質定量分析
5-12 支架組織切片分析
第六章 結論
第七章 參考文獻

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