(3.236.118.225) 您好!臺灣時間:2021/05/16 13:20
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果

詳目顯示:::

: 
twitterline
研究生:謝韻聲
研究生(外文):Yun-Sheng Hsieh
論文名稱:斑馬魚新穎消化道突變種之遺傳特性及型態分析
論文名稱(外文):Genetic analysis and morphological characterization of novel zebrafish GI (gastrointestinal tract) mutants
指導教授:何國牟
指導教授(外文):Guor-Mour Her
學位類別:碩士
校院名稱:國立臺灣海洋大學
系所名稱:生物科技研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2008
畢業學年度:96
語文別:中文
論文頁數:85
中文關鍵詞:斑馬魚消化道突變N-ethyl-N-nitrosourea(ENU)
外文關鍵詞:zebrafishgastrointestinal tract(GI) mutantN-ethyl-N-nitrosourea(ENU)
相關次數:
  • 被引用被引用:0
  • 點閱點閱:265
  • 評分評分:
  • 下載下載:28
  • 收藏至我的研究室書目清單書目收藏:0
斑馬魚的種種優點使此種模式生物已經成為實驗室中普遍的研究物種,也適合用來進行 (large-scale mutagenesis screen)。在斑馬魚中大量的突變分析是利用ENU(N-ethyl-N-nitrosourea)這個突變劑來使斑馬魚產生大量的突變,ENU是個烷化劑 (alkylating agent),會作用在公魚的精母幹細胞(spermatogonial stem cell),轉移它的乙烷基至DNA的氧或氮自由基上造成點突變。此研究對四種經過ENU注射而造成mutant的斑馬魚X-liver、Y-liver、Snake1及Snake2進行了型態的分析,發現四種mutant在5 dpf時皆因魚鰾萎縮而沉在底部不會游動,其中四種mutant的眼睛及頭部變的比較小;X-liver與Snake2的心臟有水腫的現象;而且Snake2的體軸有彎曲的情況;四種mutant的肝臟與腸道似乎都有發育不完全的情況。再利用Whole-Mount In Situ Hybridization的技術,用可以專一表現在各個消化性臟器的marker來評估mutant對斑馬魚消化性臟器發育上的影響,發現這四種mutant對肝臟、腸道、胰臟及胰島δ-細胞皆有影響。也利用多基因轉殖螢光魚LIP-αβ與mutant斑馬魚進行交配,利用螢光來觀察mutant對斑馬魚消化性臟器發育上的影響,發現在X-liver與Snake1的消化性臟器發出的螢光皆明顯的比wild type弱。這些結果顯示四種mutant皆對消化性臟器的發育造成嚴重程度不等的影響。
目錄.....I
表目錄.....III
圖目錄.....IV
中文摘要.....V
英文摘要.....VI
壹、序論.....1
一、動物模式物種 ─ 斑馬魚.....1
二、斑馬魚腸道、肝臟及胰臟的發育.....3
三、N-ethyl-N-nitrosourea (ENU)簡介.....7
四、指標基因(Marker Gene)簡介.....9
五、研究動機.....13
貳、實驗材料與方法.....15
一、實驗材料.....15
二、實驗方法.....22
參、實驗結果......33
一、Mutant斑馬魚的型態分析.....33
二、Whole-Mount In Situ Hybridization.....35
三、多基因轉殖螢光魚與Mutant斑馬魚.....42
肆、討論.....44
伍、參考文獻.....48
附表.....53
附圖.....55
Ackermann A.M. and Gannon M.. (2007). Molecular regulation of pancreatic β-cell mass development, maintenance, and expansion. J Mol Endocrinology. 38(1-2): 193-206.

Andre M., Ando S., Ballagny C., Durliat M., Poupard G., Briancon C., BabinP.J.. (2000). Intestinal fatty acid binding protein gene expression reveals the cephalocaudal patterning during zebrafish gut morphogenesis. Int J Dev Biol. 44(2): 249-252.

Argenton F., Zecchin E., Bortolussi M.. (1999). Early appearance of pancreatic hormone-expressing cells in the zebrafish embryo. Mech Dev. 87(1-2): 217-221.

Atshaves B.P., McIntosh A.M., Lyuksyutova O.I., Zipfel W., Webb W.W., Schroeder F.. (2006). Liver Fatty Acid-binding Protein Gene Ablation Inhibits Branched-chain Fatty Acid Metabolism in Cultured Primary Hepatocytes. Am J Physiol Gastrointest Liver Physiol. 290(1): G36-48.

Bang U.C., Semb S., Nojgaard C., Bendtsen F.. (2008). Pharmacological approach to acute pancreatitis. World J Gastroenterol. 14(19): 2968-2976.

Bhandari S., Watson N., Long E., Sharpe S., Zhong W., Xu S.Z., Atkin S.L.. (2008). Expression of Somatostatin and Somatostatin Receptor Subtypes 1-5 in Human Normal and Diseased Kidney. J Histochem Cytochem. 56(8): 733-743.

Biemar F., Argenton F., Schmidtke R., Epperlein S., Peers B., Driever W.. (2001). Pancreas development in zebrafish: early dispersed appearance of endocrine hormone expressing cells and their convergence to form the definitive islet. Dev Biol. 230(2): 189-203.

Chen J., Ruan H., Ng S.M., Gao C., Soo H.M., Wu W., Zhang Z., Wen Z., Lane D.P., Peng J. (2005). Loss of function of def selectively up-regulates Delta113p53 expression to arrest expansion growth of digestive organs in zebrafish. Genes Dev. 19(23): 2900-2911.

Cordes S.P.. (2005). N-Ethyl-N-Nitrosourea Mutagenesis: Boarding the Mouse Mutant Express. Microbiol Mol Biol Rev. 69(3): 426-439.

Dooley K. and Zon L.I.. (2000). Zebrafish: A model system for the study of human disease. Curr Opin Genet Dev. 10(3): 252-256.

Geisler R., Rauch G.J., Geiger-Rudolph S., Albrecht A., van Bebber F., Berger A., Busch-Nentwich E., Dahm R., Dekens M.P., Dooley C, Elli A.F., Gehring I., Geiger H., Geisler M., Glaser S., Holley S., Huber M., Kerr A., Kirn A., Knirsch M., Konantz M., Kuchler A.M., Maderspacher F., Neuhauss S.C., Nicolson T., Ober E.A., Praeg E., Ray R., Rentzsch B., Rick J.M., Rief E., Schauerte H.E., Schepp C.P., Schonberger U., Schonthaler H.B., Seiler C., Sidi S., Sollner C., Wehner A., Weiler C., Nusslein-Volhard C.. (2007). Large-scale mapping of mutations affecting zebrafish development. BMC Genomics. 8:11.

Her G.M., Yeh Y.H., Wu J.L.. (2003). 435-bp Liver Regulatory Sequence in the Liver Fatty Acid Binding Protein (L-FABP) Gene Is Sufficient to Modulate Liver Regional Expression in Transgenic Zebrafish. Dev Dyn. 227(3): 347-356.

Her G.M., Yeh Y.H., Wu J.L.. (2004). Functional Conserved Element Mediate Intestinal-Type Fatty Acid Binding Protein (I-FABP) Expression in the Gut Epithelia of Zebrafish Larvae. Dev Dyn. 230(4): 734-742.

Hill A.J., Teraoka H., Heideman W., Peterson R.E.. (2005). Zebrafish as a Model Vertebrate for Investigating Chemical Toxicity. Toxicological Sci. 86(1): 6-19.

Hirota M., Ohmuraya M., Baba H.. (2006). Genetic background of pancreatitis. Postgrad Med J. 82(974): 775-778.

Holmberg A., Olsson C., Holmgren S.. (2006). The effects of endogenous and exogenous nitric oxide on gut motility in zebrafish Danio rerio embryos and larvae. J Exp Biol. 209(Pt 13):2472-2479.

Jung J., Zheng M., Goldfarb M., Zaret K.S.. (1999). Initiation of mammalian liver development from endoderm by fibroblast growth factors. Science. 284(5422): 1998-2003.

Kennedy C.L. and O’Bryan M.K.. (2006). N-ethyl-N-nitrosourea (ENU) mutagenesis and male fertility research. Hum Reprod Update. 12(3): 293-301.

Kim H.J., Sumanas S., Palencia-Desai S., Dong Y., Chen J.N., Lin S.. (2006). Genetic Analysis of early Endocrine Pancreas Formation in Zebrafish. Mol Endocrinology. 20(1): 194-203.

Kimmel C.B., Ballard W.W., Kimmel S.R., Ullmann B., Schilling T.F.. (1995). Stages of embryonic development of the zebrafish. Dev Dyn. 203(3): 253-310.

Landrier J.F., Thomas C., Grober J., Duez H., Percevault F., Souidi M., Linard C., Staels B., Besnard P.. (2004). Statin Induction of Liver Fatty-Binding Protein (L-FABP) Gene Expression Is Peroxisome Proliferator-acivated Receptor-α-dependent. J Biol Chem. 279(44): 45512-45518.

Le Douarin N.M., Renaud D., Teillet M.A., Le Douarin G.H. (1975). Cholinergic differentiation of presumptive adrenergic neuroblasts in interspecific chimeras after heterotopic transplantations. Proc natn Acad Sci USA. 72(2): 728-732.

Mao J.D., Wu P., Yang Y.L., Wu J., Huang H.. (2008). Relationship between expression of gastrin, somatostatin, Fas/FasL and caspases in large intestinal carcinoma. World J Gastroenterol. 14(18): 2802-2809.

Mayer A.N. and Fishman M.C.. (2003). Nil per os encodes a conserved RNA recognition motif protein required for morphogenesis and cytodifferentiation of digestive organs in zebrafish. Development. 130(17): 3917-3928.

McGonnell I.M. and Fowkes R.C.. (2006). Fishing for gene function – endocrine modelling in the zebrafish. J Endocrinol. 189(3): 425-439.
Meeker N.D. and Trede N.S.. (2008). Immunology and zebrafish: Spawning new models of human disease. Dev Comp Immunol. 32(7): 745-757.

Milewski W.M., Duguay S.J., Chan S.J., Steiner D.F.. (1998). Conservation of PDX-1 structure, function, and expression in zebrafish. Endocrinology. 139(3): 1440-1449.

Nadauld L.D., Shelton D.N., Chidester S., Yost H.J., Jones D.A.. (2005). The zebrafish retinol dehydrogenase, rdh1l, is essential for intestinal development and is regulated by the tumor suppressor adenomatous polyposis coli. J Biol Chem. 280(34): 30490-30495.

Pack M., Solnica-Krezel L., Malicki J., Neuhauss S.C., Schier A.F., Stemple D.L., Driever W., Fishman M.C.. (1996). Mutations affecting development of zebrafish digestive organs. Development. 123: 321-328.

Rai K., Chidester S., Zavala C.V., Manos E.J., James S.R., Karpf A.R., Jones D.A., Cairns B.R.. (2007). Dnmt2 function in the cytoplasm to promote liver, brain, and retina development in zebrafish. Genes Dev. 21(3): 261-266.

Rawls J.F., Mahowald M.A., Goodman A.L., Trent C.M., Gordon J.I.. (2007). In vivo imaging and genetic analysis link bacterial motility and symbiosis in the zebrafish gut. Proc Natl Acad Sci U S A. 104(18): 7622-7627.

Ribatti D., Conconi M.T., Nussdorfer S.S.. (2007). Nonclassic Endogenous Novel Regulators of Angiogenesis. Pharmacol Rev. 59(2): 185-205.

Rich A., Leddon S.A., Hess S.L., Gibbons S.J., Miller S., Xu X., Farrugia G.. (2007). Kit-like immunoreactivity in the zebrafish gastrointestinal tract reveals putative ICC. Dev Dyn. 236(3): 903-911.

Roest Crollius H. and Weissenbach J.. (2005). Fish genomics and biology. Genome Res. 15(12): 1675-1682.

Rossi J.M., Dunn N.R., Hogan B.L., Zaret K.S.. (2001). Distinct mesodermal signals, including BMPs from the septum transversum mesenchyme, are required in combination for hepatogenesis from the endoderm. Genes Dev. 15(15): 1998-2009.

Sadler K.C., Amsterdam A., Soroka C., Boyer J., Hopkins N.. (2005). A genetic screen in zebrafish identifies the mutants vps18, nf2 and foie gras as models of liver disease. Development. 132(5):3562-3572.

Sadler K.C., Krahn K.N., Gaur N.A., Ukomadu C.. (2007). Liver growth in the embryo and during liver regeneration in zebrafish requires the cell cycle regulator, uhrf1. Proc Nat Acad Sci USA. 104(5):1570-1575.

Schonthaler H.B., Fleisch V.C., Biehlmaier O., Makhankov Y., Rinner O., Bahadori R., Geisler R., Schwarz H., Neuhauss S.C.F., Dahm R.. (2008). The zebrafish mutant lbk/vam6 resembles human multi-systemic disorders caused by aberrant trafficking of endosomal vesicles. Development. 135(2): 387-399.

Sivasubbu S., Balciunas D., Amsterdam A., Ekker S.C.. (2007). Insertional mutagenesis strategies in zebrafish. Genome Biology. Suppl 1: S9.

Solnica-Krezel L., Schier A.F., Driever W.. (1994). Efficient Recovery of ENU-Induced Mutations From the Zebrafish Germline. Genetics. 136(4): 1401-1420.

Warga R.M., Nu‥sslein-Volhard. (1999). Origin and development of the zebrafish endoderm. Development. 126(4): 827-838.

Wienholds E., Eeden F.V., Kosters M., Mudde J., Plasterk R.H.A., Cuppen E.. (2003). Efficient Target-Selected Mutagenesis in Zebrafish. Genome Res. 13(12): 2700-2707.

Zaret K.S.. (2002). Regulatory phases of early liver development: paradigms of organogenesis. Nat Rev Genet. 3(7): 499-512.
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top