臺灣博碩士論文加值系統

摘要
以蒸氣蒸餾法萃取柳橙果皮之精油，利用GC分析其成份，主要成份為limonene (859.98 mg/g)。經柳橙果皮中自體酵素作用後能明顯將碳氫?烯類化合物轉換成含氧?烯類化合物。以30℃作用7天，含氧?烯類化合物linalool可達11.24 mg/g。另外添加20 mM Mg2+時，linalool含量由原來11.24增至12.18 mg/g。選擇新鮮且未加Mg2+之柳橙皮精油、經30℃反應7天與添加20 mM Mg2+經30℃反應7天後所萃取之精油對Malassezia furfur做抗菌試驗，發現在相同濃度下，以添加Mg2+所得之精油對Malassezia furfur抑制效果最好。
利用醋酐乙醯化法所製備乙醯化程度為43.9%之水溶性幾丁聚醣對Malassezia furfur做抗菌試驗，1000 ppm的水溶性幾丁聚醣可使菌數比控制組下降約5 Log CFU/mL。添加1000 ppm之水溶性幾丁聚醣於1.0%經Mg2+催化後之精油乳化物中，可使菌數小於1 Log CFU/mL，其抑菌效果比單獨使用精油或幾丁聚醣為佳。

Abstract
Orange peel oil was extracted by steam distillation and the constituents were analyzed by GC. The major component was limonene (859.98 mg/g). In the oil, there was a significant conversion from hydrocarbon terpenes to oxygenated terpenes after reacting with endogenous enzymes. Through endogenous enzymatic reaction at 30℃ for 7 days, the content of linalool which is an oxygenated terpene reached 11.24 mg/g. Further adding 20 mM of Mg2+, the content of linalool increased form 11.24 up to 12.18 mg/g. Fresh orange peel oil with endogenous enzymatic reaction at 30℃ for 7 days was chosen to test its antifungal activity for Malassezia furfur. It was forword that addition of 20 mM of Mg2+ in the oil for the endogenous enzymatic reaction had the best antifungal activity.
Water soluble chitosan, prepared by acetylation with acetic anhydride, had the degree of acetylation of 43.9%. The chitosan was used to do the antifungal test of Malassezia furfur. When the concentration of the chitosan was 1000 ppm, the cell count was about 5 Log CFU/mL lower than the control. Adding 1000 ppm chitosan into 1.0% emulsified oil in which
20 mM of Mg2+ was added increased its antifungal activity for Malassezia furfur. The cell count was less than 1.0 Log CFU/mL. The antifungal activity was better than just either using orange peel oil or chitosan alone.

目 錄
摘要 I
目錄…………………………………………………………………….....III
表目錄……………………………………………………………...……VII
圖目錄………………………………………………………...………...VIII
一、前言 1
二、文獻整理 3
(一)柳橙之簡介 3
(二)精油及其萃取方式 3
1.水蒸氣蒸餾法 3
2.壓榨法 4
3.溶劑萃取法 4
4.超臨界二氧化碳萃取法 4
(三)植物體中類?烯類化合物的生合成途徑 5
(四)柳橙精油之組成成份 6
(五)精油組成成份中limonene的轉換 6
(六)精油的應用 8
(七)頭皮屑與皮屑芽胞菌 9
(八)目前市售洗髮精之抗屑作用機制 12
(九)添加茶樹精油於洗髮精中的芳香療法 13
(十)精油抗皮屑芽胞菌(Malassezia furfur)之效果 13
(十一)水溶性幾丁聚醣之製備 14
(十二)幾丁聚醣之應用 15
(十三)幾丁聚醣抗菌機制及效果 15
(十四)幾丁聚醣與精油抗菌效果之加乘性 16
三、實驗方法與材料 17
(一) 實驗材料 17
1.樣品 17
2.標準品 17
3.藥品 17
4.菌株 18
5.培養基組成成份 18
6.層析管柱 19
7.透析膜 19
8.儀器裝置 19
(二) 實驗方法………………...……………………………………21
1.柳橙皮精油之萃取 21
2.柳橙皮精油成份分析 21
3.柳橙皮經自體酵素反應後精油成份之改變 23
4.水溶性幾丁聚醣之製備 24
5.水溶性幾丁聚醣乙醯化程度之測定 24
6.抗菌活性測試 28
7.統計分析 31
四、結果與討論 32
(一) 柳橙皮精油之組成 32
(二) 柳橙皮經自體酵素反應後精油成份之改變 33
1. 溫度與時間之影響 33
2. 添加金屬離子溶液之影響 35
(三) 柳橙皮精油乳化物對Malassezia furfur之抗菌活性影響 36
1. 皮屑芽胞菌(Malassezia furfur)生長曲線 36
2. 柳橙精油乳化物抗菌試驗 37
(四) 水溶性幾丁聚醣對Malassezia furfur之抗菌活性 38
1. 水溶性幾丁聚醣濃度之影響 38
2. 添加幾丁聚醣於柳橙皮精油之抗菌活性 39
五、結論 41
六、參考文獻 42

表目錄
表一、柳橙皮精油主要成份含量 50
表二、在20℃下自體酵素作用後柳橙精油中成份含量 51
表三、在30℃下自體酵素作用後柳橙精油中成份含量 52
表四、在50℃下自體酵素作用後柳橙精油中成份含量 53
表五、不同濃度之新鮮柳橙精油對Malassezia furfur生菌數之影響 54
表六、經30℃、7天自體酵素催化之不同濃度柳橙精油對Malassezia furfur生菌數之影響 55
表七、不同濃度經添加Mg2+在30℃下自體酵素作用三天之柳橙精油對Malassezia furfur生菌數之影響 56
表八、不同濃度之水溶性幾丁聚醣對Malassezia furfur生菌數之影響 57
表九、添加水溶性幾丁聚醣於1.0%新鮮柳橙皮精油乳化物中對Malassezia furfur生菌數之影響 58
表十、添加水溶性幾丁聚醣於1.0%經添加Mg2+在30℃下自體酵素作用三天之柳橙精油乳化物中對Malassezia furfur生菌數之影響 59

圖目錄
圖一、植物體內類?烯類化合物的生成途徑。 60
圖二、Geraniol及linalool的生成途徑。 61
圖三、於20、30、50℃下自體酵素作用對limonene的含量變化。 62
圖四、於20、30、50℃下自體酵素作用對linalool的含量變化。 63
圖五、添加不同濃度之Mn2+金屬離子在30℃下自體酵素作用7天後， linalool的含量變化。 64
圖六、添加不同濃度之Mg2+金屬離子在30℃下自體酵素作用7天後， linalool的含量變化。 65
圖七、皮屑芽胞菌之生長曲線。 66
圖八、水溶性幾丁聚醣之層析圖譜。 67

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