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研究生:蔡璧如
研究生(外文):pi-ru tsai
論文名稱:龜山島熱泉週邊海域細菌之分離及其硫代謝能力分析
論文名稱(外文):Isolation of bacteria and effect of sulfur metabolism in the hydrothermal vents surrounding Kueishan Island.
指導教授:鄭學淵鄭學淵引用關係
指導教授(外文):Cheng Sha-Yen
學位類別:碩士
校院名稱:海洋大學
系所名稱:環境生物與漁業科學學系
學門:農業科學學門
學類:漁業學類
論文種類:學術論文
論文出版年:2008
畢業學年度:96
語文別:中文
論文頁數:73
中文關鍵詞:龜山島海底熱泉硫代謝
外文關鍵詞:Kueishan Islandhydrothermal ventssulfur metabolism
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在龜山島週邊海域2005年測得之營養鹽資料中表水溫平均為25.1℃;鹽度無顯著差異,平均為33.78 psu;pH值平均為7.9 ;水中硫化物含量平均只有0.8 μM。但在熱泉噴口處,水溫29.4℃,pH 5.3,硫化物667.52 μM。由此可知熱泉噴口處為一個特殊之環境,而在此環境中細菌佔重要的角色。
本研究培養此環境之細菌,將分離獲得之菌株初步挑選後進行外部型態及生理生化特性分析,經16S rRNA序列進行比對其相似菌種。其菌種為D-1相似菌屬為Dietzia屬,9-2相似菌屬為Marinobacter屬,2-11 最相似菌種為Rhodococcus屬與菌種12-4 最相似菌種為Salinicoccu屬。並針對分離之菌種對硫的代謝情形進行實驗觀察。
各菌種產生硫化物濃度中以Rhodococcus sp.反應最為顯著,於36 hr硫化物濃度達到最高157.8 μM;而其餘各菌種代謝出的濃度皆低。
Rhodococcus sp.、Dietzia sp.、Marinobacter sp.及9-3 sp.皆可氧化元素硫產生硫酸根離子。至96 hr代謝的硫酸根離子濃度分別為84.2 μM、63.8 μM、100.3 μM、132.6 μM 。
本研究亦發現Rhodococcus sp.於6-36 hr中可還原元素硫產生硫化物,且同時也可氧化元素硫產生硫酸根離子,即具disproportionation作用。
The average temperature, salinity, pH and sulfide were 25.1℃, 33.78 psu, pH 7.9 and 0.8 μM measured from surrounding of the Kueishan island in 2005. However, the temperature, pH and sulfide were 29.4℃, pH 5.3 and 667.52 μM in the hydrothermal vents. The bacteria took an important roles in this special environment.
This kind of bacteria was cultivated in middle temperature condition. Isolate strains were selected to study their morphologies and physiological characteristics. These similar bacteria were compared by using the rank of 16S rRNA. The D-1 similar bacteria was Dietzim, 9-2 similar bacteria was Marinobacter, 2-11 was Rhodococcus and 12-4 was Salinicoccu. Then the isolate strains was observed the sulfur metabolism.
The most notable reaction in each bacterium of sulfide concentration was Rhodococcus sp. It produced the highest sulfide concentration in 36 hrs. The others produced the lower sulfide concentration.
Rhodococcus sp., Dietzia sp., Marinobacter sp. and 9-3 sp all could oxidize the sulfur and produce sulfate. The metabolism concentration of sulfate during 96 hr were 84.2, 63.8, 100.3 and 132.6 μM.
This study also discovered the Rhodococcus sp. had the disproportionation function in 6-36 hr. This results indicate the Rhodococcus sp. could reduced the sulfur to sulfide and oxidize the sulfur to sulfate at the same time.
中文摘要 I
英文摘要 III
目錄 IV
謝辭 V
第一章 前言 1
第二章 文獻整理 5
第三章 龜山島週邊海域於中溫之條件下菌株之分離研究 10
第四章 各菌種對硫代謝情形之研究 18
第五章 討論 25
第六章 結論 33
第七章 參考文獻 34
表 42
圖 54
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