( 您好!臺灣時間:2023/06/10 00:57
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::


研究生(外文):Chia-Nan Chien
論文名稱(外文):Ensemble recording system and development of telemetric technology for neural signals
指導教授(外文):Fu-Shan Jaw
外文關鍵詞:bio-telemetrydata acquisitionfield potentialsmultichannelmulti unit action potentials
  • 被引用被引用:0
  • 點閱點閱:192
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
本論文的第二部份是建構了一簡單小型化無線遙測記錄系統,用於記錄大鼠自由活動時神經元的反應,其發射模組重量約只有大鼠體重的1%,所以不會對於大鼠移動時造成過多的負擔。這個系統具有長使用時間及等向性輻射等特性,滿足記錄動物自由活動反應的必要條件。另外,完成一低頻截止點到達6 Hz的調頻式接收器,以滿足記錄神經信號時所需之寬頻帶響應,其範圍包含了低頻的場電位信號。本論文詳細闡述設計這些系統時的考量因素及製作方法,希望能對其他從事相關研究的同好有所助益。
Electrophysiological techniques could help us to understand the complex behavior of neural activities. The conventional field potentials (FPs) and multi-unit activities (MUAs) recording are to complement each other. Firstly, a hybrid multichannel system was proposed to record FPs and MUAs. The detail procedures of microelectrode specification determination were described, and a custom-made electrode for the acute mapping of cerebral cortex was constructed. Moreover, a three-stage signal-conditioning unit was designed that offered low noise, low cost and excellent line-driving capabilities. The parallel architecture of the filter stage, just before the analog-to-digital (A/D) converter card, allowed the MUAs and FPs to be recorded simultaneously without further signal processing. To obtain a precision map, a 64-channel high-speed A/D card and its software program were purchased, which allowed “pseudo-synchronous” acquisition among different channels. Finally, the practical application of this system in investigating cortical responses showed that it met the requirements of ensemble recording.
Secondly, a simple miniature telemetry system for neural recording from freely moving rats was described. It weighed only 1% of the body weight of an adult rat and shows no observable effects on the movement of the animal. Together with its long recording time (more than 38 h), its isotropic nature, which was essential for working with freely moving animals, offer further advantages. A frequency-modulation receiver with a flat frequency response down to 6 Hz had been designed for wide-spectrum recording of neural signals, allowed FPs recording. The detailed design considerations and methods for implementing the system could be valuable to other neuroscience laboratories.
口試委員會審定書 I
誌謝 II
中文摘要 III
英文摘要 V
內容目錄 VII
圖目錄 IX
1. 緒論 1
1.1 前言 1
1.2 文獻回顧 3
1.3 研究動機與目的 7
2. 神經網路活動記錄系統 9
2.1 前言 9
2.2 系統架構之考量 12
2.3多通道電極之製作 18
2.4多通道信號調控單元之設計 26
2.5近乎同步資料擷取系統 31
2.6系統實際應用與測試 39
3. 無線傳輸神經活動記錄系統 44
3.1 前言 44
3.2 生物無線遙測記錄模組之設計 49
3.2.1發射模組 49
3.2.2接收模組 52
3.3 實際應用與測試結果 57
3.3.1生物無線遙測記錄系統之實際規格 57
3.3.2神經信號無線傳輸 58
4. 討論與結論 64
4.1 神經網路活動記錄系統 64
4.2 無線遙測記錄系統 68
5. 未來展望 71
附錄一 無線傳輸系統之相關原理 80
附錄二 論文著作表 90
Barth DS, Kithas J, Di S. Anatomic organization of evoked potentials in rat parietotemporal cortex: somatosensory and auditory response. J. Neurophysiol., 1993; 69: 1837-9.
Bragin A, Hetke J, Wilson CL, Anderson DJ, Engel JrJ, Buzsaki G. Multiple site silicon-based probes for chronic recordings in freely moving rats: implantation, recording and histological verification. J. Neurosci. Methods, 2000; 98: 77-82.
Brown EN, Kass RE, Mitra PP. Multiple neural spike train data analysis: state-of-the-art and future challenges. Nature Neurosci., 2004; 7: 456-61.
Buzsaki G. Large-scale recording of neuronal ensembles. Nature Neurosci., 2004; 7: 446-51.
Boguski MS and Jones AR. Neurogenomics: at the intersection of neurobiology and genome sciences. Nature Neurosci., 2004; 7 : 429-33.
Chang W-T. Develop neural signal compressor using mpeg-layer-3: a preliminary study. M.S. thesis, Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan, 2006.
Chapin JK. Using multi-neuron population recordings for neural prosthetics. Nature Neurosci., 2004; 7: 452-5.
Chen H-J. Multi-unit analysis: from single cell to neuronal ensemble. M.S. thesis. Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan, 2007.
Chen Y-Y. Current source density of field potentials in the brain. M.S. thesis, Institute of Electrical Engineering, National Taiwan University, Taipei, Taiwan, 1998.
Chen Y-Y, Kuo T-S, Jaw F-S. A laser micromachined probe for recording multiple field potentials in the thalamus. J. Neurosci. Methods, 2004; 139: 99-109.
Chien C-N, Jang J-K, Jaw F-S. Study on a ‘pseudo’ synchronous A/D card for the mapping of multi-channel signals. 2000 Conference on Biomedical Engineering Technology.
Chien C-N and Jaw F-S. Analysis of field potentials on the cerebral cortex. 2001 Conference on Biomedical Engineering Technology.
Choudhary J and Grant SG. Proteomics in postgenomic neuroscience: the end of the beginning. Nature Neurosci., 2004; 7: 440-5.
Chudler EH and Dong WK. The assessment of pain by cerebral evoked potentials. Pain, 1983; 16: 221-44.
Churchland PS and Sejnowski TJ. Perspectives on cognitive neuroscience. Science, 1988; 242: 741-5.
Deadwyler SA and Hampson RE. The significance of neural ensemble codes during behavior and cognition. Annu. Rev. Neurosci., 1997; 20: 217-44.
Di S and Barth DS. Topographic analysis of field potentials in rat vubrissa barrel cortex. Brain Res., 1991; 546: 106-12.
Fan C-L. Design and fabrication of neuroprobes for chronic recording and iontophoresis. M.S. thesis. Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan, 2007.
Fischer H, Kautz H, Kutsch W. A radiotelemetric 2-channel unit for transmission of muscle potentials during free flight of the desert locust, Schistocerca gregaria. J. Neuronsci. Methods, 1996; 64: 39-45.
Ghazanfar AA, Stambaugh CR, Nicolelis MAL. Encoding of tactile stimulus location by somatosensory thalamocortical ensembles. J. Neurosci., 2000; 20: 3761-75.
Grohrock P, Hausler U, Jurgens U. Dual-channel telemetry system for recording vocalization-correlated neuronal activity in freely moving squirrel monkeys. J. Neuronsci. Methods, 1997; 76: 7-13.
Guillory KS and Normann RA. A 100-channel system for real time detection and storage of extracellular spike waveforms. J. Neurosci. Methods, 1999; 91: 21-9.
Hodgkin AL and Huxley AF. Currents carried by sodium and potassium ions through the membrane of the giant axon of Loligo. J. Physiol., 1952; 116: 449-72
Hoogerwerf AC and Wise KD. A three-dimensional microelectrode array for chronic neural recording. IEEE Trans. Biomed. Eng., 1994; 41: 1136-46.
Insel TR, Volkow ND, Landis SC, Li TK, Battey JF, Sieving P, National Institutes of Health. Limits to growth: why neuroscience needs large-scale science. Nature Neurosci., 2004; 7: 426-7.
Jaw F-S, Yu S-N, Lee J-C, Tsao H-W, Yu H-J, Yen C-T. Interactive program for spectral and area analysis of compound action potentials of A-fiber and C-fiber. J. Neurosci. Methods, 1991; 40: 121-6.
Jaw F-S, Lin C-W, Wang C-Y. Sampling variation caused by A/D cards due to external trigger. J. Neurosci. Methods, 1995; 61: 175-8.
Jaw F-S. Optimal sampling of electrophysiological signals. Neurosci. Res. Commun., 2001; 28: 75-84.
Kralik JD, Dimitrov DF, Krupa DJ, Katz DB, Cohen D, Nicolelis MAL. Techniques for long-term multisite neuronal ensemble recordings in behaving animals. Methods, 2001; 25: 121-50.
Kramer K and Kinter LB. Evaluation and applications of radiotelemetry in small laboratory animals. Physiol. Genomics, 2003; 13: 197-205.
Lai M-L, Kuo T-S, Jaw F-S. Electrometer for intracellular and extracellular recordings. Biomed. Eng. Appl. Basis Comm., 2000; 12: 213-6.
Lamour Y, Guilbaud G, Willer JC. Rat somatosensory (SmI) cortex: II. Laminar and columnar organization of noxious and non-noxious inputs. Exp. Brain Res., 1983; 49: 46-54.
Lee C-C. Central transmission pathway and lateral thalamic processing of the rat tail inputs. Ph. D. thesis, Institute of Zoology, National Taiwan University, Taipei, Taiwan, 2002.
Liao W-L. Analysis of cortical noxious responses caused by CO2 laser stimulation in the tail of the rat and development of a mechanical noxious stimulator. M.S. thesis, Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan, 2004.
Lu M-Y. Development of a 64-channel microelectrode system. M.S. thesis, Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan, 2005.
Martone ME, Gupta A, Ellisman MH. E-neuroscience: challenges and triumphs in integrating distributed data from molecules to brains. Nature Neurosci., 2004; 7: 467-72.
Melzack R and Casey KL. Sensory, motivational, and central control determinants of pain. In Kenshalo DR, editor. The skin senses. CC Thomas, Springfield: 1968; 423-39.
Mirnics K and Pevsner J. Progress in the use of microarray technology to study the neurobiology of disease. Nature Neurosci., 2004; 7: 434-9.
Mountcastle VB. The columnar organization of the neocortex. Brain, 1997; 120: 701-22.
Narasimhan K. Scaling up neuroscience. Nature Neurosci., 2004; 7: 425.
Nicolelis MAL, Baccala LA, Lin RCS, Chapin JK. Sensorimotor encoding by synchronous neural ensemble activity at multiple levels of the somatosensory system. Science, 1995; 268: 1353-8.
Nicolelis MAL, Ghazanfar AA, Faggin B, Votaw S, Oliverira LMO. Reconstructing the engram: simultaneous multiple site, many single neuron recordings. Neuron, 1997; 18: 529-37.
Novak JL and Wheeler BC. Multisite hippocampal slice recording and stimulation using a 32 element microelectrode array. J. Neurosci. Methods, 1988; 23: 149-59.
Novak JL and Wheeler BC. A high-speed multichannel neural data acquisition system for IBM PC compatibles. J. Neurosci. Methods, 1989; 26: 239-47.
Obeid I, Nicolelis MAL, Wolf PD. A multichannel telemetry system for single unit neural recordings. J. Neuronsci. Methods, 2004; 133: 33-8.
Owens AL, Denison TJ, Versnel H, Rebbert M, Peckerar M, Shamma SA. Multi-electrode array for measuring evoked potentials from surface of ferret primary auditory cortex. J. Neurosci. Methods, 1995; 58: 209-20.
Paxinos G and Watson C. The rat brain: in stereotaxic coordinates. 4th ed. Academic Press, 1998.
Powell TPS and Mountcastle VB. Some aspect of the function organization of the cortex of the postcentral gyrus of the monkey: a correlation of findings obtained in a single unit analysis with cytoarchitecture. Bulletin of the John Hopkins Hospital, 1959; 105.
Richard CJ. Tutorial: analog data acquisition technology. Part III- sample-and-holds, instrumentation amplifier, and analog multiplexers. IEEE Micro, 1982; 20-35.
Schouenborg J, Kalliomaki J, Gustavsson P, Rosen I. Field potential evoked in rat primary somatosensory cortex (SI) by impulses in cutaneous Aβ- and C- fibers. Brain Res., 1986; 86-92.
Shaw F-Z, Chen R-F, Tsao H-W, Yen C-T. A multichannel system for recording and analysis of cortical field potentials in freely moving rats. J. Neurosci. Methods, 1999; 88: 33-43.
Skutt HR, Beschle RG, Moulton DG, Koella WP. New subminiature amplifier-transmitters for telemetering biopotentials. Electroenceph. Clin. Neurophysiol., 1967; 22: 275-7.
Szabo I, Czurko A, Csicsvari J, Hirase H, Leinekugel X, Buzsaki G. The application of printed circuit board technology for fabrication of multi-channel micro-drives. J. Neurosci. Methods, 2001; 105: 105-10.
Tecott LH and Nestler EJ. Neurobehavioral assessment in the information age. Nature Neurosci., 2004; 7: 462-6.
Teng T-K. Development of a system for the recording of electrophysiological signal in the thalamus. M.S. thesis, Institute of Biomedical Engineering, National Taiwan Univ., Taipei, Taiwan, 2003.
Tsai M-L and Yen C-T. A simple method for fabricating horizontal and vertical microwire arrays. J. Neuronsci. Methods, 2003; 131: 107-10.
Tsai Y-C. Measurement systems for electrophysiology signals — from basic biomedical recordings to clinical applications. M.S. thesis, Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan, 2003.
Van Horn JD, Grafton ST, Rockmore D, Gazzaniga MS. Sharing neuroimaging studies of human cognition. Nature Neurosci., 2004; 7: 473-81.
Van WHA and Hufschmidt M. A complete FM radio on a chip. AN192, Philips Semiconductors, 1991.
Wei C-F. Development of a multi-channel microelectrode and analysis of extra-cellular recordings. M.S. thesis, Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan, 2003.
Williams JC, Rennaker RL, Kipke DR. Long-term neural recording characteristics of wire microelectrode arrays implanted in cerebral cortex. Brain Res. Protoc., 1999; 4: 303-13.
Wilson MA and McNaughton BL. Reactivation of hippocampal ensemble memories during sleep. Science, 1994; 265: 676-9.
Xu S, Talwar SK, Hawley ES, Li L, Chapin JK. A multi-channel telemetry system for brain microstimulation in freely roaming animals. J. Neuronsci. Methods, 2004; 133: 57-63.
第一頁 上一頁 下一頁 最後一頁 top