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研究生:張慶全
研究生(外文):Ching-chuan Chang
論文名稱:利用連續式與脈衝式射頻電漿聚合含胺基之高分子薄膜及材料性質分析
論文名稱(外文):Characterization of amine-containing thin films prepared by continuous and pulsed plasma polymerization
指導教授:王孟菊
指導教授(外文):Meng-Jiy Wang
學位類別:碩士
校院名稱:國立臺灣科技大學
系所名稱:化學工程系
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:2008
畢業學年度:96
語文別:中文
論文頁數:130
中文關鍵詞:連續式電漿脈衝式電漿能率循環電漿聚合生物相容性
外文關鍵詞:continuous plasmapulsed plasmaduty cycleplasma polymerizationcell biocompatibility
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本論文之研究目的為利用含胺基之單體(丙胺、丙烯胺、丙炔胺),以連續式與脈衝式電漿製備含胺基高分子薄膜。在連續式電漿聚合方面,探討沉積時間、電漿功率對丙胺、丙烯胺、丙炔胺沉積之薄膜性質影響;在脈衝式電漿聚合方面,探討能率循環(duty cycle)對丙胺、丙烯胺、丙炔胺沉積之薄膜性質影響;並就連續式與脈衝式電漿製備之高分子薄膜,探討生物相容性與電漿參數之關係。
  由連續式電漿所聚合之高分子薄膜分析結果發現,對飽和以及不飽和之單體,其沉積速率與時間關係為一常數,且正比於電漿功率,在低功率下丙烯胺、丙炔胺具有較高的沉積效能(ng/J),由傅立葉紅外線光譜儀和X射線光電子能譜儀分析結果顯示,丙胺、丙烯胺、丙炔胺薄膜,確實含胺之官能基。同時,利用滴定方法測量胺基數目之結果,發現低電漿功率下具有較高的胺基含量。由脈衝式電漿高聚合之分子薄膜分析結果發現,單體沉積速率與能率循環成正比。經由控制能率循環,由傅立葉紅外線光譜儀和X射線光電子能譜儀分析結果顯示,在低能率循環條件下可獲得富含胺基之丙胺、丙烯胺、丙炔胺薄膜。
對於連續式和脈衝式電漿所製備之含胺基高分子薄膜,利用生物相容性測試結果為:由細胞型態觀察方面,於含胺基之電漿高分子薄膜表面,細胞能夠順利延展並呈現纖維狀形態,推論丙胺、丙烯胺、丙炔胺電漿高分子薄膜都可增進細胞附著、延展以及增生。在細胞活性分析方面,無論是利用連續式或是脈衝式電漿所製備之丙胺、丙烯胺、丙炔胺薄膜,使用纖維母細胞培養之結果,其活性都大於存在於矽基材上之活性。本研究利用連續式與脈衝式電漿,能夠在不同電漿參數下,製備之含胺基之高分子薄膜,並且對於細胞之延展以及活性有提升之作用。
The goal of this research work is to prepare amine-containing polymer thin films by both continuous and pulsed plasma polymerization methods. Three monomers containing various unsaturated structures (propylamine, allylamine, propargylamine) are chosen to provide amine functionalities. The effects of operational parameters such as deposition time, applied power and duty cycle on the physical-chemical properties of polymerized thin films were evaluated by studying their chemical compositions, deposition rates, surface morphology and functionalities.
For both continuous and pulsed plasma polymerizations, the deposition rates were found to be independent of deposition time and proportional to the applied power for three monomers. The surface morphology obtained by AFM showed island structures with nearly constant roughness. This indicates that the surfaces of the thin films prepared by plasma are very uniform. For lower applied power, there exist higher deposition rates for allylamine and propargylamine monomers due to the unsaturated structures. The FTIR and XPS results demonstrated that the amine functional groups have been successfully incorporated onto the thin films for three monomers. The amine derivatization experiments were used to quantify the amine functionalities. The results showed that higher amine concentration can be created at the condition of lower power for both continuous and pulsed plasma polymerization processes. For pulsed plasma polymerization processes, the deposition rate is proportional to the duty cycle.
In order to examine the biocompatibility of amine-containing thin films prepared by plasma polymerizations, the cell behaviors of fibroblasts on these films were monitored. The cell morphology observed by SEM showed that the amine-containing thin films can effectively assist cell spreading. The cell viability was quantified by MTT tests and the results showed that thin films prepared by all these three monomers
can enhance cell viability up to 2.3 folds comparing with the control sample.
中文摘要I
英文摘要II
誌謝III
總目錄IV
表目錄VI
圖目錄VIII
第一章緒論1
1.1 研究背景1
1.2 研究目標3
第二章 文獻回顧5
2.1 電漿簡介5
2.1.1 電漿定義5
2.1.2 電漿源種類5
2.1.3 直流電漿5
2.1.4 電容式偶和射頻電漿6
2.1.5 脈衝式電漿6
2.2 薄膜沉積7
2.3 電漿聚合8
2.3.1 電漿聚合理論9
2.3.2 電漿聚合參數12
2.4 電漿聚合含胺基薄膜於生醫領域的應用14
第三章 研究方法與儀器原理16
3.1 研究目的16
3.2 實驗步驟17
3.2.1 實驗設備17
3.2.2 單體流量校正19
3.2.3 電漿聚合薄膜21
3.2.4 物理性質分析23
3.2.5 化學性質分析24
3.3 儀器原理25
3.3.1 石英晶體微量天秤25
3.3.2 掃描式電子顯微鏡25
3.3.3 原子力顯微鏡26
3.3.4 X-射線光電子能譜儀27
3.3.5 傅立葉紅外線光譜儀28
3.3.6 紫外線-可見光分光光譜儀28
3.3.7 接觸角測量儀29
3.4 細胞培養29
3.5 實驗藥品與儀器30
第四章 結果與討論32
4.1 電漿高分子薄膜特性之研究32
4.1.1 沉積時間對電漿高分子薄膜特性之影響32
4.1.2 電漿功率對電漿高分子薄膜特性之影響53
4.2 利用脈衝式電漿沉積含胺基高分子薄膜77
4.2.1 脈衝式能率循環與沉積速率之關聯分析77
4.3 含胺基之電漿高分子薄膜對生物相容性之影響97
4.3.1 電漿高分子薄膜對生物型態之影響98
4.3.2 能率循環對生物型態之影響100
4.3.3 電漿高分子薄膜對生物活性之影響104
4.3.4 能率循環對生物活性之影響105
第五章 結論與建議109
第六章 參考文獻112
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