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研究生:陳欣郁
研究生(外文):Shin-Yu Chen
論文名稱:石蓮與刺蔥萃取物之抗菌能力分析
論文名稱(外文):Studies on the antibacterial activity of extracts ofZanthoxylum ailanthoides Sieb&Zucc. and Graptopetalum paraguayense E.Walther
指導教授:謝尤敏
指導教授(外文):You-Miin Hsieh
學位類別:碩士
校院名稱:靜宜大學
系所名稱:食品營養研究所
學門:醫藥衛生學門
學類:營養學類
論文種類:學術論文
論文出版年:2008/07/
畢業學年度:96
語文別:中文
論文頁數:75
中文關鍵詞:刺蔥抗菌益生菌石蓮
外文關鍵詞:antibacterialG. paraguayenseZ. ailanthoidesprobiotic
相關次數:
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因為抗生素過度濫用,許多細菌都產生抗藥性,且抗生素對病原菌或益生菌都有抑制作用,以具有抗菌作用之草本植物,取代抗生素,可減少細菌產生抗藥性,也避免因使用過多抗生素,抑制對人體健康有益之益生菌生長。本研究分析刺蔥Zanthoxylum ailanthoides Sieb&Zucc.與石蓮Graptopetalum paraguayense E. Walther萃取物對微生物生長的影響,萃取物共六種,為水萃和50%酒萃的刺蔥莖部、葉部與石蓮,測試病原菌為葡萄球菌屬(Staphylococcus spp.)、大腸桿菌(Escherichia coli)、產氣莢膜桿菌(Clostridium perfringens)、轉糖鏈球菌(Streptococcus mutans)和痤瘡丙酸桿菌(Propionibacterium acnes),以洋菜擴散試驗分析抑菌能力,並測試萃取物之最小抑菌濃度(minimum inhibitory concentration; MIC)與最小殺菌濃度測試(minimum bactericidal concentration; MBC)。亦分析萃取物對乳酸桿菌屬菌株(Lactobacillus spp.)及比菲德氏菌屬菌株(Bifidobacterium spp.)生長之影響。結果顯示,酒萃刺蔥葉部抑制Staphylococcus spp.之活性最為顯著,抑制直徑最大可達23 mm,MIC與MBC分別為6.25和12.5 mg/ml,石蓮萃取物抑制葡萄球菌屬之活性,抑制直徑最大為14 mm,MIC與MBC分別為12.5和25 mg/ml,抑制病原菌活性最低者為酒萃和水萃刺蔥莖部,萃取物抑制其他病原菌之能力較低;10 μg抗生素Ampicillin和Gentamicin抑制病原菌能力大部分都比萃取物優良,但是刺蔥葉部萃取物對S. aureus 12653之抑制能力優於Ampicillin;不同溶劑萃取之萃取物,以酒萃抑制病原菌能力較水萃之萃取物佳。萃取物對益生菌生長之影響,以酒萃及水萃刺蔥葉部和酒萃石蓮萃取物會抑制益生菌生長,但酒萃、水萃刺蔥莖部和水萃石蓮萃取物有促進益生菌生長的情形。本研究結果顯示刺蔥與石蓮萃取物抑制Staphylococcus spp.生長之能力最強,或許可以作為取代抗生素之天然抑菌劑,並且有作為益生質之潛力。
Antibiotic resistance has become a serious problem because of the abuse of antibiotics. To antibacterial activity of antibiotics inhibits both pathogens and probiotics. The use plant extracts could be of great significance in replace of antibiotics to reducing the antibiotic resistance and avoiding inhibition to probiotics. The aim of this study was to investigate on the antibacterial activity of the water and 50% ethanol extracts of Zanthoxylum ailanthoides Sieb&Zucc. and Graptopetalum paraguayense E. Walther against Staphylococcus spp., Escherichia coli, Clostridium perfringens, Streptococcus mutans and Propionibacterium acnes by using agar diffusion method and evaluating the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). In addition, the growth of Lactobacillus spp. Bifidobacterium spp. affected by extracts also was determined. The leaf extracts of Z. ailanthoides had the more inhibitory effect against at Staphylococcus spp. The inhibition zone was 23 mm. The MIC and MBC was 6.25 and 12.5 mg/ml, respectively. The inhibition zone of the extracts of G. paraguayense aganist Staphylococcus spp. was 14 mm. The MIC and MBC was 12.5 and 25 mg/ml, respectively. The stem extract of Z. ailanthoides had the lowest inhibition activity. The extracts have the lowest inhibition activity at other pathogens. The antibacterial activity of 10 μg Ampicillin and Gentamicin was better than most of the tested extracts. The antibacterial activity of the leaf extracts of Z. ailanthoides was better than Ampicillin against S. aureus 12653. The ethanolic extracts were more active than the aqueous extracts. In the test of extracts affecting the growth of probiotics, the leaf extracts of Z. ailanthoides and the ethanolic extracts of G. paraguayense showed the inhibitory action on the growth of probiotics. However, the stem extracts of Z. ailanthoides and the aqueous extracts of G. paraguayense enhance the growth of probiotics. The results obtained in this study demonstrated the aqueous and ethanolic extracts of Z. ailanthoides and G. paraguayense have the highest antibacterial effect on Staphylococcus spp. These extracts might be used as natural antibacterial substance and have the potential as prebiotic compound.
目次
頁次
中文摘要 6
英文摘要 8
第壹章 前言 10
第貳章 文獻整理 12
第一節、刺蔥簡介 12
第二節、石蓮簡介 13
第三節、病原菌簡介 14
(一)葡萄球菌簡介 15
(二)大腸桿菌簡介 15
(三)梭狀芽孢桿菌簡介 16
(四)痤瘡丙酸桿菌簡介 17
(五)轉糖鏈球菌簡介 18
第四節、益生菌簡介 18
第參章 實驗目的 20
第肆章 材料與方法 21
第一節、實驗材料 21
(一)菌株 21
(二)培養基 22
(三)其他材料 24
(四)菌種保存 25
第二節、實驗方法 25
第一部份 抑菌圈分析分析 25
(一)病原菌培養 25
(二)益生菌培養 25
(三)萃取物製備與濃度 26
(四)抑菌能力對照品 26
(五)抑菌圈抑菌活性之評估 26
第二部分 病原菌之最小抑菌濃度與最小殺菌濃度 27
(一)萃取物製備與濃度 27
(二)病原菌培養 27
(三)最小抑菌濃度與最小殺菌濃度評估 28
第三部份 萃取物對益生菌生長之影響 28
(一)萃取物製備與濃度 28
(二)益生菌培養 28
(三)分析方法 28
第伍章 結果與討論 30
第一部份 抑菌圈能力分析結果 30
(一)萃取物對葡萄球菌屬之抑制能力分析 30
(二)萃取物對大腸桿菌之抑制能力分析 31
(三)萃取物對產氣莢膜桿菌之抑制能力分析 31
(四)萃取物對痤瘡丙酸桿菌之抑制能力分析 31
(五)萃取物對轉糖鏈球菌之抑制能力分析 32
(六)益生菌之抑菌圈結果 32
第二部分 最小抑菌濃度與最小殺菌濃度結果 37
(一)葡萄球菌屬 37
(二)大腸桿菌 37
(三)產氣莢膜桿菌 37
(四)痤瘡丙酸桿菌 38
(五)轉糖鏈球菌 38
第三部分萃取物對益生菌生長之影響 40
第陸章 結論 43
第柒章 參考文獻 68



































表目錄
表一、病原菌簡介 44
表二、病原菌簡介 45
表三、萃取物和抗生素以洋菜擴散法抑制Staphylococcus spp.之抗菌活性 46
表四、萃取物和抗生素以洋菜擴散法抑制EAggEC之抗菌活性 47
表五、萃取物和抗生素以洋菜擴散法抑制Cl. perfringens、P. acnes和Strep. mutans之抗菌活性 48
表六、抗生素以洋菜擴散法分析Lactobacillus spp.之抗菌活性 49
表七、抗生素以洋菜擴散法分析Bifidobacterium spp.之抗菌活性 50
表八、萃取物之pH值 51
表九、萃取物抑制S. aureus最小抑菌濃度與最小殺菌濃度結果 52
表十、萃取物抑制Staphylococcus spp.最小抑菌濃度與最小殺菌濃度結果 53
表十一、萃取物抑制E. coli最小抑菌濃度與最小殺菌濃度結果 54
表十二、萃取物抑制Cl. perfringens、P. acnes和Strep. mutans最小抑菌濃度與最小殺菌濃度結果 55
表十三、六種萃取物對益生菌生長之影響列表 56








圖目錄
圖一、刺蔥圖片 57
圖二、石蓮圖片 57
圖三、萃取物抑制S. caprae 13911之抑制圈結果 58
圖四、抑菌圈試驗中萃取物對益生菌生長之影響 59
圖五、添加六種萃取物對L. reuteri 14625生長之影響 60
圖六、添加六種萃取物對L. reuteri 14625生長之影響 61
圖七、添加六種萃取物對L. acidophilus 14097生長之影響 62
圖八、添加六種萃取物對L. farciminis 14043生長之影響 63
圖九、添加六種萃取物對B. longum 14634生長之影響 64
圖十、添加六種萃取物對B. longum 14634生長之影響 65
圖十一、添加六種萃取物對B. infantis 14603生長之影響 66
圖十二、添加六種萃取物對B. indicum 14674生長之影響 67
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