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研究生:王昭能
研究生(外文):Charles C-N Wang
論文名稱:水稻T-DNA科技服務中心專屬水稻功能性基因體種原庫資訊系統之建立
論文名稱(外文):The establishment of a rice functional genomics information system in T-DNA Tagged Rice Service Center (TTRSC)
指導教授:許承瑜許承瑜引用關係
指導教授(外文):Phillip C-Y Sheu
學位類別:碩士
校院名稱:亞洲大學
系所名稱:生物資訊學系碩士班
學門:生命科學學門
學類:生物訊息學類
論文種類:學術論文
論文出版年:2008
畢業學年度:96
語文別:中文
論文頁數:77
中文關鍵詞:T-DNA兩翼序列(Flanking sequence)水稻資料庫T-DNA兩翼序列(Flanking sequence)水稻資料庫T-DNA兩翼序列(Flanking sequence)水稻資料庫
外文關鍵詞:T-DNAFlanking sequencericedatabaseT-DNAFlanking sequencericedatabaseT-DNAFlanking sequencericedatabase
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中研院分生所余淑美博士團隊,利用T-DNA 標定技術,在台農67號水稻中得到超過60,000個突變株,一方面進行插入位置之DNA定序,另方面持續繁殖以獲得T2或T3種子,同時也提供其它研究人員進行功能性基因體研究。本研究就是延伸這項工作,收集已繁殖T2種子及其基本資料、農藝性狀及相關T-DNA插入之兩翼序列。TTRSC之水稻功能性基因體種原庫資訊系統,提供水稻功能性基因研究用種原的查詢及管理,資料庫內含部份T2種子之基本資料、種子性狀及相關T-DNA插入之兩翼序列。提供給水稻基因研究人員快速的查詢及研究材料的取得。水稻功能性基因體種原庫資訊系統功能分為:會員功能、查詢/物流功能、庫存功能及智慧財產權追蹤,以提高這些珍貴的水稻種原的利用效率,並控管品系編號之正確庫存位置及存量,以及使用者提領及使用者管考,並紀錄種子庫存過程的基本資料及所有材料轉移資料,對這些材料有興趣的研究人員,可以進入本系統查詢種子相關資料,查詢可以利用T-DNA水稻編號(ID)進行查詢,並且可以利用T-DNA水稻種子性狀進行查詢(種子長、子寬、種子高、十粒重)以及線上申請種子。
TTRSC 資料庫目前庫存有7844 筆水稻種子性狀相關資料。本系統可提供對水稻基因功能有興趣的研究人員,可以快速查詢T-DNA 水稻相關資料,也可以很方便的找到研究的材料,加速水稻功能性基因體學之研
Prof. Yu produced more then 60,000 mutated plants used by T-DNA insertion rice NT-67. On one hand, Prof. she has generated the DNA sequences in Flanking of T-DNA the inserted place. On the other hand, she is continuously growing the mutated genomics for magnate the T2 or T3 T-DNA lines. At the same time, Prof. she provides all those T-DNA seeds for other researchers to carry out the study functional gene systems. This study, this rice functional genomics information system provides both the searching and management for the study of rice functional genomics. which extends what Prof. Yu is doing, This research ponies an information system content, the characteristics and shape, and the relevant flanking sequence in the database.
Therefore, the database provides some of the information mentioned above for the researcher to have quick searching and references.
The functions of this Rice Functional Genomics Information System are: (1). Member registration function; (2). Searching/query function; (3). Storage function and (4). intellectual right management. The reasons for these functions are that it is necessary to increase the efficiency of using these valuable rice genetic resources. At the same time, the database can control the place and storage of the inventory., Recording the basic information in the storage process as well as all the information of material transfer are also considered in this study. For those researchers who are interested in this field, they can use this system searching for the relevant information of the seeds. they are interesting The researchers can use the T-DNA rice ID for searching. In addition, they can use the characteristic and morphology of T-DNA rice seed for their searching (the length, width, height and weight of seed) and query for the seeds on line.
The TTRSC database currently sofas the characteristics for more than 7844 lines of rice T-DNA seeds. Those researchers who are interested in the functional gene of rice can quickly search for the information of T-DNA by this database. Additionally, it is exceptionally convenient in finding the materials for research, which is helpful in accelerating the research on rice functional genomics.
目錄
誌謝
第一章 緒論
第一節 研究背景 -1
第二節 研究動機及目的 -2
第三節 論文架構 -3
第二章 前人研究
第一節 國際水稻基因定序計畫 -5
第二節 水稻功能性基因研究 -8
第三節 T-DNA(transfer-DNA) -11
第四節 T-DNA 插入突變點兩側序列資料庫-12
第五節 台灣水稻功能性基因之發展-13
第六節 目前世界各國水稻功能性基因資料庫之發展-14
第三章 材料及方法
第一節 研究材料-15
第二節 研究方法-19
第四章 研究結果
第一節 系統建立結果 -39
第二節 使用者使用介面結果 -40
第三節 系統管理者介面結果 -43
第四節 目前資料庫蒐集結果 -48
第五節 水稻種子推廣結果 -49
第六節 水稻種子物流結果 -50
IV
第五章 討論
參考文獻
商標聲明-58
附件一、Material TransferAgreement-57
附件二、目前國內主動寄送電子郵件名單-64
附件三、申請種子編號-66
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International Rice Genome Sequencing Project : http://rgp.dna.affrc.go.jp/IRGSP/
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