臺灣博碩士論文加值系統

本論文報告雙股DNA上鄰近的胜肽結合位置之間的合作性研究。利用設計好的新型模組之胜肽如KHypH-12、KHypS-12對5，端標記32P的pBR322 158mer Watson片段和互補的135mer Crick片段進行結合，顯示其有不同程度的合作性指標(希爾係數)。使用不同的胜肽與設計過的只含有一個胜肽結合位置5’-d(AAAA)-d(TTTT)-3’的雙股DNA - S-81做結合，用以探討鄰近的胜肽結合位置之間的異位影響。另一方面，選擇RPyH-12這個胜肽在三種不同的反應溫度(25˚C, 31˚C, 和 37˚C)進行合作性的探討，發現其具有不同程度的合作性指標(希爾係數)，推論溫度對於胜肽結合到核酸序列上的結合位置之合作性是有著指標性的影響。

This thesis reports a study to disclose the cooperativity between neighboring peptide binding sites in duplex DNA. Designed peptides with novel motifs such as KHypH-12, KHypS-12 display different magnitude of cooperative index (Hill coefficients) on binding to a 5’-32P-labeled 158-mer and a complementary 5’-32P-labeled 135-mer duplex. A designed duplex DNA (S-81) bearing a single peptide binding site 5’-d(AAAA)-d(TTTT)-3’ was used to probe the allosteric interaction between neighboring binding sites for different peptides. On the other hand, a selected peptide RPyH-12 displays different magnitude of cooperative index (Hill coefficients) at three incubation temperatures: 25˚C, 31˚C, and 37˚C, suggesting that temperature has a marked effect on cooperative peptide binding on DNA recognition sites.

目 錄

中文摘要 I
英文摘要 II
目錄 III
圖目錄 IV
表目錄 VII
縮寫目 VIII
第一章 前言 1
第二章 實驗原理 5
第三章 材料與方法 17
第四章 結果與討論 30
第五章 結論 75
第六章 參考文獻 76

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