本研究係以中台灣高美濕地之沉積底泥中，培養出降解海藻產甲烷之中溫厭氧混合菌群。此厭氧菌群以龍鬚菜之海藻多糖為主要碳源，以批次方式培養在不同溫度、鹽度、pH值、生長輔因子(yeast extract, peptone)、碳源、不同海藻多醣來源之條件下生長。根據結果顯示此厭氧生物降解海藻產甲烷之最佳溫度、鹽度與pH值分別為35oC、0 %與pH 7.1，而最佳碳源(龍鬚菜)添加濃度為4 g/L；此外生長輔因子(yeast extract，peptone)皆有促進之結果，而以yeast extract有較佳的刺激生長作用；再由最佳條件下進行不同藻類之降解產甲烷研究，此厭氧菌群無法顯著地將馬尾藻之藻體降解，但可利用其溶出之海藻多醣，而龍鬚菜則可被降解至破碎薄片狀；以最佳化反應條件培養時，有最高之海藻降解速率為0.84±0.08 g．L-1．day-1，且可生成最高1431.20±99.65 mmole之甲烷生成，其甲烷生成速率為210.02±24.15 mmole．day-1，反應液之主要中間產物為醋酸，最高可生成29.96±1.04 mmole醋酸，產率為16.11±0.14 mmole．g seaweed-1。

Mesophilic anaerobic biodegradation of seaweed by mixed microorganisms from Kaomei wetland sediment at Central Taiwan was investigated in this study. Gracilaria was used as the sole carbon source for methane production by the mixed culture. Batches of the mixed culture were grown at different conditions such as different temperatures, sodium chlorides, pH’s, growth factors, carbon sources and various sources of algal polysaccharides from seaweed to determine the optimal conditions for methane production from seaweed bioconversion. The optimal temperature, sodium chloride concentration and pH value for bioconversion of seaweed to methane was 35oC, 0 % and pH 7.1, respectively. The optimal substrate concentration for methane production was 4 g/L of Gracilaria. Both growth factors yeast extract and peptone could stimulate bacterial growth with yeast extract giving the better effect. Sargassum could not be degraded by the mixed culture at the optimal conditions,. The highest seaweed degradation rate, methane yield and methane production rate by the mixed culture were 0.84±0.08 g．L-1．day-1, 1431.20±99.65 mmole and 210.02±24.15 mmole．day-1 respectively. Acetic was found to be the major product in the liquid phase during Gracilaria biodegradation. The highest acetate yield is 16.11±0.14 mmole．g seaweed-1.

第一章 前言
1.1 研究動機
1.2 研究目的
第二章 文獻回顧
2.1 再生能源
2.1.1 能源現況
2.1.2 生質能(Biomass energy)
2.1.3 生質能與碳循環
2.1.4 生質能源產生方式
2.2 海藻(Seaweed)
2.2.1 海藻之利用
2.2.2 臺灣海藻資源
2.2.3 海藻組成
2.3 海藻多醣來源
2.3.1 紅藻(Rhodophyceae)
2.3.2 綠藻(Chlorophyceae)
2.3.3 褐藻(Phaeophyceae)
2.4 海藻多醣結構
2.4.1 洋菜(Agar)
2.4.2 褐藻膠(Alginate)
2.4.3 鹿角菜膠(Carrageenan)
2.5 分解海藻多醣之agarase
2.5.1 來源
2.5.2 分類與分解方式
2.6 海藻厭氧生物轉化(Anaerobic bioconversion of seaweed)
2.7 生物甲烷(Biogas)燃料與應用
2.8 厭氧醱酵化學
2.9 厭氧生物處理之影響因子
2.9.1 溫度
2.9.3 鹽度
2.9.2 pH值
2.9.3 輔因子
2.9.4 碳源種類
第三章 實驗材料與方法
3.1 實驗流程設計
3.2 菌種來源採樣
3.3 菌種培養
3.3.1 Hungate 除氧系統
3.3.2 厭氧操作台
3.3.3 厭氧培養基
3.3.4 菌群培養
3.4 分析方法與儀器
3.4.1 菌群生長分析
3.4.2 甲烷生成量分析
3.4.3 海藻重量分析
3.4.4 總糖分析
3.4.5 還原糖分析
3.4.6 液相成分分析
3.5 轉化甲烷程序最適化探討
3.5.1 生長溫度
3.5.2 生長鹽濃度
3.5.3 生長pH值
3.5.4 生長輔因子
3.5.5 不同海藻濃度
3.5.6 不同藻類利用
第四章 實驗結果與討論
4.1 龍鬚菜降解混合菌群增富(Enrichment)
4.1.1 海藻降解測試
4.1.2 甲烷生成測試
4.2 反應程序最適條件探討
4.2.1 溫度
4.2.2 鹽濃度
4.2.3 pH值
4.2.4 生長輔因子添加(Growth factor)
4.3 藻類濃度對甲烷生成之影響探討
4.4 不同藻類之利用探討
4.6 最佳反應條件下之液相產物探討
第五章 結論與建議
5.1 結論
5.2 建議
第六章 參考文獻

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