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研究生:蔡政諺
研究生(外文):Cheng-Yen Tsai
論文名稱:關節液成份分子於不同測試條件下對人工關節材料之摩擦影響
論文名稱(外文):The Effects of Synovial Fluid Compositions on the Friction of Artificial Joint Materials under the different test Condition
指導教授:方旭偉方旭偉引用關係
口試委員:劉宣良蔡德華張至宏
口試日期:2008-07-10
學位類別:碩士
校院名稱:國立臺北科技大學
系所名稱:化學工程研究所
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:2008
畢業學年度:96
語文別:中文
論文頁數:82
中文關鍵詞:鈷鉻鉬合金金超高分子量聚乙烯摩擦係數
外文關鍵詞:CoCrMoUHMWPEfriction coefficient
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現今人工關節植入物的材料主要是由鈷鉻鉬合金金與超高分子量聚乙烯組合。然而,超高分子量聚乙烯所產生的磨耗顆粒會引起人體一連串的免疫反應,進而引發骨溶解現象,最後造成人工關節鬆脫。當人工關節作動時,兩關節對磨表面的摩擦機制會因接觸壓力與存在於關節界面的關節液分子不同而有所不一樣。因此,對於人工關節材料在不同荷重與不同關節液分子的潤滑機制需進一步的研究探討,以了解關節液分子在摩擦系統中所扮演的角色。
本研究利用摩擦測試系統,針對關節液分子白蛋白(albumin)、γ-球蛋白(γ-golbulin)、磷脂質(lipid)、玻尿酸(hyaluronic acid)及多成份關節液分子進行摩擦測試。由實驗結果發現,單成份關節液分子在5MP的接觸壓力下摩擦係數比0.25MPa小;其中以玻尿酸各別在兩者接觸壓力下所獲得摩擦係數最小。然而,關節液為多成份分子的液體,分子之間彼此交互作用會產生不同的潤滑機制。因此,我們進一步的來了解雙成份及多成份關節液分子對人工關節潤滑機制的影響。實驗結果發現,雙成份潤滑液在接觸壓力5MPa摩擦系統中,磷脂質混合白蛋白與γ-球蛋白的摩擦係數較混合玻尿酸高。另一方面,將白蛋白、γ-球蛋白、磷脂質及玻尿酸配製成生理濃度混合時,發現在沒有添加白蛋白和γ-球蛋白的摩擦係數最低;而沒有添加玻尿酸所獲得的摩擦係數最高。可能是因玻尿酸的存在可以促進兩對磨表面的潤滑,降低摩擦力。
文獻得知蛋白質的存在會使人工關節摩擦係數增加。從實驗結果發現,變質白蛋白的摩擦係數比沒變質的白蛋白高,且兩者摩擦係數隨接觸壓力的增加而降低。摩擦過後的白蛋白經SDS-PAGE實驗發現,其分子量有明顯改變。我們推測白蛋白經摩擦過後,其分子之間的共價鍵被破壞,進而影響其摩擦係數。因此,我們針對關節液含量最豐富的白蛋白在人工關節摩擦測試系統中必須做更深入的了解。
Joint prosthesis is mainly composed of cobalt-chromium-molybdenum (Co-Cr-Mo) and ultra-high molecular weight polyethylene (UHMWPE). However, the wear particles of UHMWPE are generated in the peri-prosthetic tissue may induce the innate immune response and osteolysis which lead to loosing of the total joint replacement. The motion of joints involves several lubrication models under CoCrMo-on-UHMWPE articulating surface between different nominal contact pressure and synovial fluid molecules. Therefore, in order to understand the role of the lubricating mechanism of synovial fluid, we should further study the influence of the different normal load and synovial fluid molecules on the tribological behaviors of artificial joints.
In this study, we applied tribological test lubricant to albumin, γ-globulin, lipid, hyaluronic acid and multi-components in the synovial fluid. The result of the friction coefficient was showed lower under the contact pressure of 5MPa than 0.25MPa for one-component lubrication. The friction coefficient is lowest in the hyaluronic acid under the two contact pressure individually. However, multi-component in the synovial fluid interacts with each other, also influence the lubricating mechanism. Thereby, we have to investigate double components and multi-components synovial fluid lubrication mechanism of artificial joint. It was demonstrated that the friction coefficient were the lowest when lipid mixed hyaluronic acid than mixed albumin andγ-globulin under contact pressure of 5MPa. On the other hand, mixing the albumin, γ-globulin, lipid and hyaluronic acid are composed of physical concentration, it shows that the friction coefficient were lower without protein, but without hyaluronic acid were not. It could reduce friction force when the hyaluronic acid existed.
The friction coefficient increase for the artificial joints when the lubricant contains proteins. It shows that the denatured albumin friction coefficient is higher than the nature albumin, and increase the contact pressure leads to the friction coefficient decrease. Changes in the molecular weight of albumin were measured by SDS-PAGE after friction experiment with a tribometer. We consequence of the covalent bond of albumin is destroyed by frictional shearing actions and then effects of friction coefficient to artificial joints. Thereby, the final goal of the study is to understand the abundant albumin in the synovial fluid under the frictional test system of the artificial joints.
中文摘要…………………………………………………………………….…………i
英文摘要.......................................................................................................................iii
誌謝...............................................................................................................................v
目錄…………………………………………………………………………………...vi
表目錄………………………………………………………………………...………ix
圖目錄………………………………………………………………………………....x
第一章 緒論…………………………………………………………………………1
1.1研究動機……………………………………………………………………..4
第二章 文獻回顧……………………………………………………………………6
2.1人工關節介紹………………………………………………………………..6
2.2超高分子量聚乙烯…………………………………………………………12
2.3人工關節摩擦潤滑學研究…………………………………………………15
2.4關節液分子對人工關節材料之摩擦研究…………………………………17
2.5人工關節摩擦測試方法……………………………………………………24
第三章 問題定義與研究方法…………………………..…………………………30
3.1 問題定義…………………………………………………………...………30
3.1.1人工關節元件摩擦與磨損…………………………...……………30
3.1.2 關節液分子對人工關節元件之摩擦行為……………..…………30
3.1.3 關節液分子的變化………………………………..………………31
3.2研究方法…….……………………………………………………………32
3.2.1 摩擦測試方法評估關節液分子之摩擦行為………..……………32
3.2.2 荷重大小對關節液分子之摩擦行為……………..………………32
第四章 摩擦測試實驗………………………………..……………………………34
4.1 目的……………………………………………………………...…………34
4.2 微摩擦磨損測試機………………………………………………………34
4.2.1 材料準備…….……………………………………………………34
4.2.2 實驗步驟………………………………………………….………35
4.2.3 實驗數據分析………………………………………….………37
第五章 關節液分子對摩擦行為的影響………………………………….………38
5.1 目的………………………………………………………………..………38
5.2 關節液分子白蛋白………………………………………………..………38
5.3 關節液分子γ-球蛋白………………………………………….…………40
5.4 關節液分子玻尿酸……………………………………………...…………42
5.5 關節液分子磷脂質………………………………………………...………44
5.6 不同關節液成份分子對摩擦係數影響………………………………...…46
5.7 關節液分子濃度對摩擦係數影響………………………………………...49
第六章 多成份關節液分子對摩擦行為的影響…………………………………..53
6.1 目的………………………………………………………………………...53
6.2 雙成份關節液分子對摩擦行為的影響…………………………………...53
6.2.1 磷脂質添加白蛋白對摩擦係數影響……………………………..53
6.2.2 磷脂質添加γ-球蛋白對摩擦係數影響………………………….55
6.2.3 磷脂質添加玻尿酸對摩擦係數影響…………..…………………56
6.3 仿生物潤滑液對摩擦係數影響…………………………………...………59
第七章 白蛋白分子摩擦行為探討………………………………………………..62
7.1 目的………………………………………………………………………...62
7.2 變質白蛋白製備….……………………………………………………...62
7.2.1 蛋白質結構測量…………………………………………………..63
7.3 Pin-on-disc摩擦測試結果…………………………………………….64
7.4 十二烷基磺酸鈉-聚丙烯醯胺膠電泳………...…………………………64
7.5 討論……………………………………………………………………….66
第八章 結論…………………………………………………………………………71
8.1 關節液分子對摩擦行為影響……………………………………………...71
8.2 多成份關節液分子對摩擦行為影響……………………………………...72
8.3白蛋白分子對摩擦行為探討………………………………………………73
文獻參考……………………………………………………………………………..74
附錄A 藥品試劑…………………………………………………………………….81
附錄B 實驗器材…………………………………………………………………….82
Affatato, S., Fernandes, B., Tucci, A., Esposito, L., Toni, A. "Isolation and morphological characterisatio of UHMWPE wear debris generated in vitro," Biomaterials, vol.22, 2001, pp.2325-2331.
Brockett, C., Williams, S., Jin, Z., Isaac, G., Fisher, J. "Friction of total hip replacements with different bearings and loading conditions," Journal of Biomedical Materials Research-Part B Applied Biomaterials, vol.81(2), 2007, pp.508–515.
Bergmanna, G., Graichena, F., Rohlmanna, A., Verdonschotb, N., van Lenthe, G. H. "Frictional heating of total hip implants. Part 2: finite element study," Journal of Biomechanics, vol.34, 2001, pp.429–435.
Cooper, J. R., Dowson, D., Fisher, J. "The effect of transfer film and surface roughness on the wear of lubricated ultra-high molecular weight polyethylene," Clinical Materials, vol.14, 1993, pp.295-302.
Chandrasekaran, M., Loh, N. L. "Effect of counterface on the tribology of UHMWPE in the presence of proteins," Wear, vol.250, 2001, pp. 237–241.
Chandrasekaran, M., Wei, L. Y., Venkateshwaran, K. K., Batchelor, A. W., Loh, N. L. "Tribology of UHMWPE tested against a stainless steel counterface in unidirectional sliding in presence of model synovial fluids: part 1," Wear, vol.223, 1998, pp.13–21.
Crescenzi, V., Taglienti, A., Pasquali-Ronchetti, I. "Supramolecular structures prevailing in aqueous hyaluronic acid and phospholipid vesicles mixtures: an electron microscopy and rheometric study," Colloids and Surfaces A: Physicochem. Eng. Aspects, vol.245, 2004, pp.133–135.
Fang, H. W., Hsu, S. M., Sengers, J. V. "Ultra-High Molecular Weight Wear Particle Effects on Bioactivity, "NIST SP1002,Washington:NIST,2003.
Fang, H. W., Yang, C. B., Chang, C. H., Huang, C. H., Liu, H. L., and Fang, S. B. "The Potential Role of Phagocytic Capacity in the Osteolytic Process Induced by Polyethylene Wear Particles," The Journal of International Medical Research, vol.34, 2006, pp.655-664.
Fang, H. W., Shih, M. L., Zhao, J. H., Huang, H. T., Lin, H. Y., Liu, H. L., Chang, C. H., Yang, C. B., Liu, H. C. "Association of polyethylene friction and thermal unfolding of interfacial albumin molecules," Applied Surface Science, vol.253, 2007, pp.6896-6904.
Forsey, R. W., Fisher, J., Thompson, J., Stone, M. H., Bell, C., Ingham, E. "The effect of hyaluronic acid and phospholipid based lubricants on friction within a human cartilage damage model." Biomaterials, vol.27, 2006, pp.4581–4590.
Fujikawa, Y., Itonaga, I., Kudo, O., Hirayama, T., Taira, H. "Macrophages that have phagocytosed particles are capable of differentiating into functional osteoclasts," Modern Rheumatol, vol.15, 2005, pp.346–351.
Gale, L. R., Coller, R., Hargreaves, D. J., Hill, B. A., Crawford, R. "The role of SAPL as a boundary lubricant in prosthetic joints," Tribology International, vol.40, 2007, pp. 601–606.
Gale, L. R., Chen, Y., Hills, B. A., Crawford, R. "Boundary lubrication of joints: Characterization of surface-active phospholipids found on retrieved implants," Acta Orthopaedica, vol.78, 2007, pp.309-314.
Gispert, M. P., Serro, A. P., Colaco, R., Saramago, B. "Friction and wear mechanisms in hip prosthesis: Comparison of joint materials behaviour in several lubricants," Wear, vol.260, 2006, pp.149–158.
Green, T. R., Fisher, J., Stone, M., Wroblewski, B. M., Ingham, E. "Polyethylene particles of a ‘critical size’ are necessary for the induction of cytokines by macrophages in vitro," Biomaterials, vol.19, 1998, pp.2297-2302.
Graves, S., Davidson, D., Ingerson, L. "AUSTRALIAN ORTHOPAEDIC ASSOCIATION NATIONAL JOINT REPLACEMENT REGISTRY", 2004.
Hills, B. A., and Crawford, R. W. "Normal and Prosthetic Synovial Joints Are Lubricated by Surface-Active Phospholipid," The Journal of Arthroplasty, Vol.18, 2003, pp.499-505.
Huskisson, E. C., Donnelly, S. "Hyaluronic acid in the treatment of osteoarthritis of the knee," Rheumatology, vol.38, 1999, pp.602-607.
Higaki, H., Murakami, T., Nakanishi, Y., Miura, H., Mawatari, T., Inamoto, Y. "The lubricating ability of biomembrane models with dipalmitoyl phosphatidylcholine and γ-globulin," Journal of Engineering in Medicine, vol.212, 1998, pp.337-346.
Heubergera, M. P., Widmera, M. R., Zobeley, E., Glockshuber, R., Spencer, N. D. "Protein-mediated boundary lubrication in arthroplasty," Biomaterials, vol.26, 2005, pp.1165–1173.
Ingram, J. H., Stone, M., Fisher, J., Ingham, E. "The influence of molecular weight, crosslinking and counterface roughness on TNF-alpha production by macrophages in response to ultra high molecular weight polyethylene particles," Biomaterials, vol.25, 2004, pp.3511–3522.
Kanaga Karuppiah, K. S., Sundararajan, S., Xu, Z. H., Li, X. "The effect of protein adsorption on the friction behavior of ultra-high molecular weight polyethylene," Tribology Letters, Vol.22, 2006, pp.181-188.
Kitano, T., Ateshian, G. A., Mowa, V. C., Kadoyab, Y., Yamanob, Y. "Constituents and pH changes in protein rich hyaluronan solution affect the biotribological properties of artificial articular joints," Journal of Biomechanics, vol.34, 2001, pp.1031–1037.
Krause, W. E., Bellomo, E. G., Colby, R. H. "Rheology of sodium hyaluronate under physiological conditions," Biomacromolecules, vol.2, 2001, pp.65-69.
Liu J., Yang L., Y. Li, Pan D. and Hopfinger A. J. "Constructing plasma protein binding model based on a combination of cluster analysis and 4D-fingerprint molecular similarity analyses," Bioorganic & Medicinal Chemistry, vol.14, 2006, pp.611–621.
Lin, H. Y., Bumgardner, J. D. "In vitro biocorrosion of Co–Cr–Mo implant alloy by macrophage cells,"Journal of Orthopaedic Research, vol.22, 2004, pp.1231-1236.
Long, M., Rack, H. J. "Titanium alloys in total joint replacement-a materials science perspective," Biomaterials, vol.19, 1998, pp.1621-1639.
Mazzucco, D., Spector, M. "The role of joint fluid in the tribology of total joint arthroplasty," Clinical Orthopaedics and Related Research, vol.429, 2004, pp.17-32.
Mishina, H., Kojima, M. "Changes in human serum albumin on arthroplasty," Wear, vol.265. 2008, pp.655–663.
Mori, S., Naito, M., Moriyama, S. "Highly viscous sodium hyaluronate and joint lubrication," International Orthopaedics, vol.26, 2002, pp.116-121.
Nakashima, K., Sawae, Y., and Murakami, T. "Influence of protein conformation on frictional properties of poly (vinyl alcohol) hydrogel for artificial cartilage," Tribology Letters, vol. 26, 2007, pp.145-151.
Nakashima, K., Sawae, Y., Murakami, T. "Effect of conformational changes and differences of proteins on frictional properties of poly(vinyl alcohol) hydrogel," Tribology International, vol.40, 2007, pp.1423–1427.
Ozturka, H. E., Stoffelb, K. K., Jonesa, C. F., Stachowiak, G. W. "The effect of surface-active phospholipids on the lubrication of osteoarthritic sheep knee joints: Friction," Tribology Letters, vol.16, 2004, pp.283-289.
Pico, G. A. "Thermodynamic features of the thermal unfolding of human serum albumin," International Journal of Biological Macromolecules, vol.20, 1997, pp.63-73.
Pickard, J. E., Fisher, J., Ingham, E., Egan, J. "Investigation into the effects of proteins and lipids on the frictional properties of articular cartilage," Biomaterials, vol.19, 1998, pp.1807-1812.
Rwei, S. P., Chen, S. W., Mao, C. F., Fang, H. W. "Viscoelasticity and wearability of hyaluronate solutions," Biochemical Engineering Journal, vol.40, 2008, pp.211–217.
Shi, B., Ajayi, O. O., Fenske, G., Erdemir, A., Liang, H. "Tribological performance of some alternative bearing materials for artificial joints," Wear, vol.255, 2003, pp. 1015–1021.
Saikko, V. "Effect of lubricant protein concentration on the wear of Ultra-high molecular weight polyethylene sliding against a CoCr counterface," Journal of Tribology, vol.125, 2003, pp.638-642.
Saikko, V., Ahlroos, T. "Phospholipids as boundary lubricants in wear tests of prosthetic joint materials," Wear, vol.207, 1997, pp.86-91.
Saikko, V. "Effect of contact pressure on wear and friction of ultra-high molecular weight polyethylene in multidirectional sliding," Journal of Engineering in Medicine, vol.220, 2006, pp.723-731.
Sawae, Y., Murakami, T., Chen, J. "Effect of synovia constituents on friction and wear of ultra-high molecular weight polyethylene sliding against prosthetic joint materials," Wear, vol.216, 1998, pp.213-219.
Sawae, Y., Yamamoto, A., Murakami, T. "Influence of protein and lipid concentration of the test lubricant on the wear of ultra molecular weight polyethylene," Trbiology International, vol.41, 2008, pp.648-656.
Trunfio-Sfarghiua, A. M., Berthier, Y., M. Meurisse, H., Rieu, J. P. "Multiscale analysis of the tribological role of the molecular assemblies of synovial fluid. Case of a healthy joint and implants," Tribology International, vol.40, 2007, pp.1500–1515.
Taglienti, A., Cellesi, F., Crescenzi, V., Sequi, P., Valentini, M., Tirelli, N. "Investigating the interactions of hyaluronan derivatives with biomolecules. The use of diffusional NMR techniques," Macromolecular Bioscience vol.6, 2006, pp.611-622.
Thomson, N. H., "The substructure of immunoglobulin G resolved to 25 kDa using amplitude modulation AFM in air," Ultramicroscopy, vol.105, 2005, pp.103-110.
Vermeer, A. W. P., Bremer, M. G. E. G., Norde, W. "Structural changes of IgG induced by heat treatment and by adsorption onto a hydrophobic Te£on surface studied by circular dichroism spectroscopy, "Biochimica et Biophysica Acta, vol.1425, 1998, pp.1-12.
Wang, A., Essner, A., Polineni, V. K., Stark, C., Dumbleton, J. H. "Lubrication and wear of ultrahigh molecular weight polyethylene in total joint replacements," Tribology International, vol.31, 1998, pp.17–33.
Widmer, M. R., Heuberger, M., Vörös, J., Spencer, N. D. "Influence of polymer surface chemistry on frictional properties under protein-lubrication conditions: implications for hip-implant design," Tribology Letters, vol.10, 2001, pp.111-116.
Wlker, P. S., and Gold, B. L. "The tribology (friction, lubrication and wear) of all metal artificial hip joint," Wear, vol.17 ,1971, pp. 285-299.
Walker, P. S., Blunn, G. W., Lilley, P. A. "Wear testing of materials and surfaces for total knee replacement," Journal of Biomedicat Materials Research, vol.33, 1996, pp.159-175
Wang, Y. Q., Li, J. "Sliding wear behavior and mechanism of ultra-high molecularweight polyethylene," Materials Science and Engineering, vol.266, 1999, pp.155–160.
Yao, J. Q., Blanchet, T. A., Murphy, D. J., Laurent, M. P. "Effect of fluid absorption on the wear resistance of UHMWPE orthopedic bearing surfaces," Wear, vol.255, 2003, pp.1113–1120.
Yao, J. Q., Laurent, M. P., Johnson, T. S., Blanchard, C. R., Crowninshield, R. D. "The influences of lubricant and material on polymer/CoCr sliding friction," Wear, vol.255, 2003, pp.780–784.
Young, S. K., Lotito, M. A., Keller, T. S. "Friction reduction in total joint arthroplasty," Wear, vol.222, 1998, pp.29-37.
Zhou, Y. S., Ikeuchi, K., Ohashi, M. "Comparison of the friction properties of four ceramic materials for joint replacements," Wear, vol.210, 1997, pp.171-177.
傅宇輝,骨科醫學原理及應用,台北:大中國圖書公司,1987。
王盈錦,生物醫學材料,台北:合計圖書出版社,2002。
李偉銘,摩擦過程引發生物潤滑液變化對軟骨及人工關節材料摩擦性質之影響, 碩士論文,國立台北科技大學或學工程所,台北,2006。
施孟琳,關節潤滑液分子對人工關節材料摩擦行為之影響,碩士論文,國立台北科技大學或學工程所,台北,2007。
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