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研究生:林怡仁
研究生(外文):Yi-Jen Lin
論文名稱:台灣產山螞蝗屬植物之鑑定(一)
論文名稱(外文):Identification of Desmodium Species in Taiwan (Ⅰ)
指導教授:邱泰惠邱泰惠引用關係
學位類別:碩士
校院名稱:中國醫藥大學
系所名稱:藥學系碩士班
學門:醫藥衛生學門
學類:藥學學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:129
中文關鍵詞:山螞蝗屬
外文關鍵詞:Desmodium
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山螞蝗屬植物屬於豆科,全世界約有350種,主要分布於熱帶和亞熱帶區域,其在醫療方面具重要價值。
台灣的山螞蝗屬植物有18種,大多生長在中低海拔地區,除了作綠肥及牧草使用外,亦有某些被當藥用,但在使用上卻常有混用或誤用的情形。
為改善此情況,本研究透過不同方法對10種山螞蝗屬植物加以鑑定。首先,以感官法及掃描式電子顯微鏡確定外部形態。其次,進行組織切片,觀察並比較內部構造異同,同時建立標準生藥組織圖譜。最後,本研究利用聚合酶鏈鎖反應 (polymerase chain reaction;PCR) ,將核糖體DNA的內轉錄間隔 (internal transcribed spacer;ITS) 區域放大並定序。
研究結果顯示,許多外形特徵及組織特徵都可作為鑑定上之依據。另外,這些植物的ITS區域長度為585-620bp,此區包含ITS1、5.8S rRNA gene及ITS2三部分,其長度分別為194-237bp、164bp及210-227bp,G+C 百分比分別為61.8-67.1%、52.4或53.0%及58.3-65.3%。進一步分析發現,各物種間ITS區域之序列相似度為78-98%。本研究亦歸納出其序列保留區形式為:GGCGC-(5 to 6n)- GCGCCAAGGAX (X表A、T或G) 。
利用上述方法,能有效鑑別這些植物,為其深入研究、臨床應用或生藥資源開發,提供一簡單、方便而可信的鑑定參考依據。
The genus Desmodium belongs to the family Leguminosae. There are about 350 species in the world and mainly distribute in the tropical and subtropical zone. They have important value in medicine.
There are 18 species of the genus Desmodium in Taiwan. They mostly grow in medium and low altitude areas. Besides being used for green manure and forage, some of them can be used as herbal medicine. However, the situation of mixed or missed use is very common.
To improve the condition, the study was to identify 10 species of the genus Desmodium by different methods. First, confirming the morphological appearances by sense and scanning electron microscope observation. Second, sectioning them to find and compare the histological characteristics. The standard maps of pharmacognostical histology were also set up at the same time. Finally, polymerase chain reaction (PCR) techniques were used to amplify internal transcribed spacer (ITS) regions of ribosomal DNA and then the sequences were recognized.
The study results showed that many morphological and histological features could be as bases of identification. Moreover, the length of ITS regions of these species was 585-620bp, including 194-237bp of ITS1, 164bp of 5.8S rRNA gene, and 210-227bp of ITS2. The G+C contents of ITS1, 5.8S rRNA gene and ITS2 were 61.8-67.1%, 52.4 or 53.0%, and 58.3-65.3%. Further analysis revealed that the sequence similarity of ITS regions between these species was 78-98%. The form of their conserved sequences : GGCGC-(5 to 6n)- GCGCCAAGGAX (X is A, T, or G) was also generalized in the study.
It could effectively identify these species by the methods and provided a simple, convenient, and trusty reference of identification for further studies, clinical application or pharmacognostical development.
略字解…………………………………………………………………………………Ⅱ
圖目錄…………………………………………………………………………………Ⅲ
表目錄…………………………………………………………………………………Ⅴ
中文摘要………………………………………………………………………………Ⅵ
英文摘要………………………………………………………………………………Ⅶ
第一章 緒言………………………………………………………………………… 1
第二章 總論………………………………………………………………………… 2
第一節 山螞蝗屬植物的藥用植物學考察…………………………………… 2
第二節 台灣產山螞蝗屬植物的藥用植物學考察…………………………… 13
第三節 山螞蝗屬植物的化學成分考察……………………………………… 25
第四節 山螞蝗屬植物的藥理作用考察……………………………………… 28
第三章 材料與方法………………………………………………………………… 30
第一節 實驗材料、試藥與儀器……………………………………………… 30
第二節 實驗方法……………………………………………………………… 36
第四章 結果與討論………………………………………………………………… 41
第一節 外部形態鑑定………………………………………………………… 41
第二節 內部組織鑑定………………………………………………………… 74
第三節 ITS序列鑑定………………………………………………………… 100
第五章 結論…………………………………………………………………………116
參考文獻…………………………………………………………………………… 123
1. 中國科學院中國植物志編輯委員會:中國植物志 (第四十一卷),科學出版社,北京,14-47,1995。
2. 李時珍:本草綱目,國立中國醫藥研究所,台北,825,1988。
3. 呂福原、毆辰雄、呂金誠:台灣樹木解說 (2),行政院農業委員會,台北,69-81,1998。
4. 劉和義、楊遠波、呂勝由、施炳霖:臺灣維管束植物簡誌 (第三卷),行政院農業委員會,台北,48-51、69-73,2000。
5. Editorial Committee of the Flora of Taiwan 2nd ed.:Flora of Taiwan. 2nd ed. (3), Botany Dept. of National Taiwan University, Taipei, 251-270, 1993.
6. Editorial Committee of the Flora of Taiwan 2nd ed.:Flora of Taiwan. 2nd ed. (6), Botany Dept. of National Taiwan University, Taipei, 64, 2003.
7. 中國科學院中國植物志編輯委員會:中國植物志 (第三十九卷),科學出版社,北京,1-2,1988。
8. 中國科學院中國植物志編輯委員會:中國植物志 (第四十卷),科學出版社,北京,1-6,1994。
9. 甘偉松:台灣藥用植物誌 (卷上),國立中國醫藥研究所,台北,280-285,1985。
10. 高木村:台灣藥用植物手冊,南天書局,台北,218-219,1988。
11. 邱年永、張光雄:原色台灣藥用植物圖鑑 (2),南天書局,台北,102-103,1998。
12. 邱年永、張光雄:原色台灣藥用植物圖鑑 (5),南天書局,台北,98,1998。
13. 張永勳、何玉玲、邱年永、陳忠川:台灣原住民藥用植物彙編,行政院衛生署中醫藥委員會,台北,189-193,2002。
14. 林宜信等:台灣常用藥用植物圖鑑 (2),行政院衛生署中醫藥委員會,台北,106-108,2002。
15. 全國中草藥匯編編寫組:全國中草藥匯編 (上冊),人民衛生出版社,北京,89、105,1983。
16. 全國中草藥匯編編寫組:全國中草藥匯編 (下冊),人民衛生出版社,北京,768,1990。
17. 四川勻藹茖顜@編寫組:四川中藥志 (第一卷),四川人民出版社,成都,214,1980。
18. 楊文乾:神奇草藥大圖鑑 (4),新潮社文化事業有限公司,台北,302-305,2007。
19. 吳進錩:天然彩色 台灣藥草 (2),南村書局,台北,94,1982。
20. Mishra P. K., Singh N., Ahmad G., Dube A., Maurya R.:Glycolipids and other constituents from Desmodium gangeticum with antileishmanial and immunomodulatory activities. Bioorganic & Medicinal Chemistry Letters 15(20):4543-4546;2005.
21. Ghosal S.:Occurrence of psychodelic substances in some Indian medicinal plants. Planta Medica 21(2):200-209;1972.
22. Avasthi B. K., Tewari J. D.:A preliminary phytochemical investigation of Desmodium gangeticum. Journal of the American Pharmaceutical Association 44:625-629;1955.
23. Ghosal S., Bhattacharya S. K.:Desmodium alkaloids. II. Chemical and pharmacological evaluation of D. gangeticum. Planta Medica 22(4):434-440;1972.
24. Ghosal S., Srivastava R. S., Bhattacharya S. K., Debnath P. K.:Desmodium alkaloids. IV. Chemical and pharmacological evaluation of Desmodium triflorum. Planta Medica 23(4):321-329;1973.
25. Purushothaman K. K., Kishore V. M., Narayanaswami V., Connolly J. D.:Structure and stereochemistry of gangetin, a new pterocarpan from Desmodium gangeticum (Leguminosae). Journal of the Chemical Society C 20(13):2420-2422;1971.
26. Purushothaman K. K., Chandrasekharan S., Balakrishna K., Connolly J. D.:Gangetinin and desmodin, two minor pterocarpanoids of Desmodium gangeticum. Phytochemistry 14(4):1129-1130;1975.
27. Ghosal S., Srivastava R. S., Banerjee P. K., Dutta S. K..:Alkaloids of Desmondium triflorum. Phytochemistry 10(12):3312-3313;1971.
28. Ghosal S., Banerjee P. K., Banerjee S. K.:A general method for the isolation of naturally occurring water-soluble bases. Phytochemistry 9(2):429-433;1970.
29. Yadava R. N., Reddy K. I. S.:Novel bioactive flavonol glycoside from Desmodium gangeticum DC. Journal of the Indian Chemical Society 80(12):1179-1182;2003.
30. Siddiqui Z. N., Zaman A.:Chemical constituents of Desmodium sequax. Indian Journal of Chemistry. 37B(10):1081-1082;1998.
31. Lewis K. C., Maxwell A. R., McLean S., Reynolds W. F., Enriquez R. G.:Room-temperature (1H, 13C) and variable-temperature (1H) NMR studies on spinosin. Magnetic Resonance in Chemistry 38(9):771-774;2000.
32. Ghosal S., Srivastava R. S.:β-Phenethylamine, tetrahydroisoquinoline and indole Alkaloids of Desmodium tiliaefolium. Phytochemistry 12(1):193-197;1973.
33. Ghosal S., Banerjee S. K., Bhattacharya S. K., Sanyal A. K.:Chemical and pharmacological evaluation of Desmodium pulchellum. Planta Medica 21(4):398-409;1972.
34. Tsanuo M. K., Hassanali A., Hooper A. M., Khan Z., Kaberia F., Pickett J. A., Wadhams L. J.:Isoflavanones from the allelopathic aqueous root exudate of Desmodium uncinatum. Phytochemistry 64(1):265-273;2003.
35. Guchu S. M., Yenesew A., Tsanuo M. K., Gikonyo N. K., Pickett J. A., Hooper A. M., Hassanali A.:C-methylated and C-prenylated isoflavonoids from root extract of Desmodium uncinatum. Phytochemistry 68(5):646-651;2007.
36. Adinarayana D., Syamasundar K. V.:Occurrence of a rare diholosylflavone, 2"-O-glucosylvitexin in Desmodium triflorum. Current Science 51(19):936-937;1982.
37. Govindarajan R., Vijayakumar M., Shirwaikar A., Rawat A. K. S., Mehrotra S., Pushpangadan P.:Antioxidant activity of Desmodium gangeticum and its phenolics in arthritic rats. Acta Pharmaceutica 56(4):489-496;2006.
38. Iwu M. M., Jackson J. E., Tally J. D., Klayman D. L.:Evaluation of plant extracts for antileishmanial activity using a mechanism-based radiorespirometric microtechnique (RAM). Planta Medica 58(5):436-441;1992.
39. Singh N., Mishra P. K., Kapil A., Arya K. R., Maurya R., Dube A.:Efficacy of Desmodium gangeticum extract and its fractions against experimental visceral leishmaniasis. Journal of Ethnopharmacology 98(1-2):83-88;2005.
40. Raj R. K.:Screening of indigenous plants for anthelmintic action against human Ascaris lumbricoides: Part--II. Indian Journal of Physiology and Pharmacology 19(1):47-49;1975.
41. Kurian G. A., Yagnesh N., Kishan S. R., Paddikkala J.:Methanol extract of Desmodium gangeticum roots preserves mitochondrial respiratory enzymes, protecting rat heart against oxidative stress induced by reperfusion injury. Journal of Pharmacy and Pharmacology 60(4):523-530;2008.
42. Kurian G. A., Philip S., Varghese T.:Effect of aqueous extract of the Desmodium gangeticum DC root in the severity of myocardial infarction. Journal of Ethnopharmacology 97(3):457-461;2005.
43. Jabbar S., Khan M. T. H., Choudhuri M. S. K.:The effects of aqueous extracts of Desmodium gangeticum DC. (Leguminosae) on the central nervous system. Pharmazie 56(6):506-508;2001.
44. Rathi A., Rao C. V., Ravishankar B., De S., Mehrotra S.:Anti-inflammatory and anti-nociceptive activity of the water decoction Desmodium gangeticum. Journal of Ethnopharmacology 95(2-3):259-263;2004.
45. Kawshik K. C., Achinto S., Sitesh C. B., Joydeb K. K.:Analgesic and anti-inflammatory activities of Desmodium triflorum DC. Journal of Biological Sciences 5(5):581-583;2005.
46. Govindarajan R., Vijayakumar M., Rao C. V., Shirwaikar A., Kumar S., Rawat A. K. S., Pushpangadan P.:Antiinflammatory and antioxidant activities of Desmodium gangeticum fractions in carrageenan-induced inflamed rats. Phytotherapy Research 21(10):975-979;2007.
47. Govindarajan R., Asare-Anane H., Persaud S., Jones P., Houghton P. J.:Effect of Desmodium gangeticum extract on blood glucose in rats and on insulin secretion in vitro. Planta Medica 73(5):427-432;2007.
48. Joshi H., Parle M.:Antiamnesic effects of Desmodium gangeticum in mice. Yakugaku Zasshi 126(9):795-804;2006.
49. Prasad M. V. V., Balakrishna K., Carey M. W.:Hepatoprotective activity of roots of Desmodium gangeticum (Linn.) DC. Asian Journal of Chemistry 17(4):2847-2849;2005.
50. Dharmani P., Mishra P. K., Maurya R., Chauhan V. S., Palit G.:Desmodium gangeticum: a potent anti-ulcer agent. Indian Journal of Experimental Biology 43(6):517-521;2005.
51. Govindarajan R., Rastogi S., Vijayakumar M., Shirwaikar A., Rawat A. K. S., Mehrotra S., Pushpangadan P.:Studies on the antioxidant activities of Desmodium gangeticum. Biological & Pharmaceutical Bulletin 26(10):1424-1427;2003.
52. 毛紹春、李竹英、李聰:山螞蝗屬3種植物的抗氧化性能研究。 雲南大學學報(自然科學版) 29(4):393-397;2007。
53. 王峰、楊連春、劉敏、呂敏、程言亮、賈�琠�:抗蛇毒中草藥拮抗ET-1和S6b作用的初步研究。 中國中藥雜誌 22(10):620-622;1997。
54. Latha P., Govindasamy S., Balakrishna K.:Effect of gangetin on fertility of male rats. Phytotherapy Research 11(5):372-375;1997.
55. Latha P., Govindasamy S.:Gangetin-a reproductive inhibitor in male rats. Phytotherapy Research 11(6):466-468;1997.
56. Dellaporta S. L., Wood J., Hicks J. B.:A plant DNA minipreparation: version II. Plant Molecular Biology Reporter 1(4):19-21;1983.
57. Kato A., Nakajima T., Yamashita J., Yakura K., Tanifuji S.:The structure of the large spacer region of the rDNA in Vicia faba and Pisum sativum. Plant Molecular Biology 14(6):983-993;1990.
58. Waldron J., Dunsmuir P., Bedbrook J.:Characterization of the rDNA repeat units in the Mitchell Petunia genome. Plant Molecular Biology 2(2):57-65;1983.
59. Barker R. F., Harberd N. P., Jarvis M. G., Flavell R. B.:Structure and evolution of the intergenic region in a ribosomal DNA repeat unit of wheat. Journal of Molecular Biology 201(1):1-17;1988.
60. D''Ovidio R.:Nucleotide sequence of a 5..8S rDNA gene and of the internal transcribed spaces from Populus deltoides. Plant Molecular Biology 19(6):1069-1072;1992.
61. Fritz G. N., Conn J., Cockburn A., Seawright J.:Sequence analysis of the ribosomal DNA internal transcribed spacer 2 from populations of Anopheles nuneztovari (Diptera: Culicidae). Molecular Biology and Evolution 11(3):406-416;1994.
62. Vogler A. P., DeSalle R.:Evolution and phylogenetic information content of the ITS-1 region in the tiger beetle Cicindela dorsalis. Molecular Biology and Evolution 11(3):393-405;1994.
63. Tautz D., Tautz C., Webb D., Dover G. A.:Evolutionary divergence of promoters and spacers in the rDNA family of four Drosophila species. Implications for molecular coevolution in multigene families. Journal of Molecular Biology 195(3):525-542;1987.
64. Baldwin B. G.:Molecular phylogenetics of Calycadenia (Compositae) based on its sequences of nuclear ribosomal DNA: chromosomal and morphological evolution reexamined. American Journal of Botany 80(2):222-238;1993.
65. 林文煜:利用核醣體內轉錄區間序列之種間特異性寡核苷酸探針快速檢測方法鑑定三種柴胡。 碩士論文,清華大學,新竹,6-7,2004。
66. Schaal B. A., Learn G. H.:Ribosomal DNA variation within and among plant
populations. Annals of the Missouri Botanical Garden 75(4):1207-1216;1988.
67. 蔡奇助、白佳惠、蔡素蕙、黃勝忠:烏來杜鵑5.8S rRNA基因與內轉錄間隔區之選殖及分析。 臺中區農業改良場研究彙報 63:1-11;1999。
68. Chaw S. M., Long H., Wang B. S., Zharkikh A., Li W. H.:The phylogenetic position of Taxaceae based on 18S rRNA sequences. Journal of Molecular Evolution 37(6):624-630;1993.
69. Liu J. S., Schardl C. L.:A conserved sequence in internal transcribed spacer 1 of plant nuclear rRNA genes. Plant Molecular Biology 26(2):775-778;1994.
70. 李昭瑩:台灣產油柑属(Phyllanthus)植物之生藥學研究。 博士論文,中國醫藥大學,台中,153,2005。
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