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研究生:蔡幸津
研究生(外文):Hsing-Chin Tsai
論文名稱:紅景天萃取液應用於燒燙傷大鼠動物模式之探討
論文名稱(外文):Study of Rhodiola extracts application on scaldal injury in a rat modal
指導教授:劉家全劉家全引用關係楊彩秀楊彩秀引用關係
指導教授(外文):Chia-Chyuan LiuTsai-Hsiu Yang
學位類別:碩士
校院名稱:嘉南藥理科技大學
系所名稱:化妝品科技研究所
學門:民生學門
學類:美容學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:81
中文關鍵詞:抗發炎紅景天萃取液抗氧化燒燙傷
外文關鍵詞:anti-inflammatoryRhodiola extractanti-oxidationburns
相關次數:
  • 被引用被引用:0
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  • 收藏至我的研究室書目清單書目收藏:2
有燒燙傷文獻指出,在熱水燙傷的動物實驗中會產生氧自由基,造成
血液及皮膚產生一連串的免疫反應,包括氧自由基會造成脂質過氧化( lipid
peroxidation )過程中會產生具毒性之醛類化合物-丙二醛(malondialdehyde;
MDA)最後造成細胞死亡;另外於體內的巨噬細胞及單核球會產生許多發
炎前因子細胞介質素一( interleukin-1β; IL-1β )和腫瘤壞死因子( tumor
necrosis factor ;TNF-a),最後導致燒燙傷休克及遠端器官的衰竭,而本研
究旨在確定可能具有保護作用的薔薇紅景天,治療燒燙傷後的氧化性傷害
及發炎反應產生的發炎指數( IL-1β )和( TNF-α)以及總抗氧化力( oxygen
radical absorbance capacity;ORAC )、脂質過氧化物( MDA )的改變。
紅景天是中國傳統用藥之一,目前常用的景天科紅景天屬植物為薔薇
紅景天(Rhodiola rosea)。主要有研究顯示紅景天根經由甲醇萃取在細胞實
驗中具有抗氧化之特性,這種具有活性的化合物已經被鑑定為紅景天苷
( Salidorside )及酪胺醇( p-tyrosol ),這兩種結構皆為phenylethanol 的衍生
物,其中salidroside 具有抗疲勞、抗缺氧、及抗微波輻射作用, p-tyrosol
則有抗氧化之功能。故本論文研究主題是以大鼠燒燙傷模式,利用紅景天
萃取液外敷使用針對抗氧化、抗發炎的評估來探討是否具有療效。
實驗首先確立燒燙傷動物模式,利用S.D. (Sprague- Dawley) 大鼠麻醉
後進行90℃熱水燙傷10 秒以及15 秒,在燙傷後分為兩組,各在24 小時及
48 小時後施行股靜脈插管手術以採集血液,檢測其脂質過氧化物-丙二醛
( MDA )之變化。結果顯示,於10 秒燙傷時間下48 小時後血清中的MDA
值顯著性增加證實此條件下其氧化性傷害顯著。
實驗進一步利用紅景天甲醇萃取液塗抹於S.D.大鼠燙傷患部,每日兩
次,於48 小時後採血液及皮膚組織並檢測其中的TNF-α IL-1β 和ORAC
以及MDA,目前實驗結果為燙傷給藥處理48 小時後,血清中的ORAC 以
及MDA 值均有明顯改善,另外在皮膚檢測其發炎指數( TNF-α IL-1β )具
有顯著性減少。結論:由實驗結果推測紅景天甲醇萃取液可能經由減少脂
質過氧化及增加體內總抗氧化力並減少皮膚中的發炎因子( TNF-α IL-1β )
而達到燒燙傷害後所造成的保護作用。
Literature pointed out that there are burns, scalded in hot water in the
animal experiments will produce oxygen free radicals, resulting in a series of
blood and skin of the immune response, including oxygen free radicals can
cause lipid peroxidation (lipid peroxidation) process will have a toxicity of
aldehydes - MDA (malondialdehyde; MDA) the final result of cell death; In
addition to macrophages in vivo and produce monocyte factor in many
inflammatory cells in a medium Su-(interleukin-1β; IL-1β) and tumor necrosis
factor (tumor necrosis factor; TNF-a, finally led to burn shock and distant
organ failure, and this study was to determine the possible protective effect of
Rhodiola Rosa, treatment after burn injury and oxidative inflammation resulting
from inflammation of the index (IL-1β) and (TNF-a, as well as the total
antioxidant activity (oxygen radical absorbance capacity; ORAC ), lipid
peroxide (MDA) changes.
And Rhodiola is one of the traditional Chinese medicine, the current
commonly used Crassulaceae Rhodiola Rhodiola plants for the roses (Rhodiola
rosea). Major studies have shown that methanol extracts of Rhodiola root
through experiments in the cell has antioxidant properties, the active compounds
have been identified as salidroside and p-tyrosol, these two structures are
derivatives of phenylethanol, which salidroside with anti-fatigue, anti-hypoxia
and the role of anti-microwave radiation, p-tyrosol have antioxidant function.
Therefore, the theme of this paper is to study burn model in rats, the use of
Rhodiola extract for external use anti-oxidation, anti-inflammatory evaluation of
whether to explore the effect.
First of all, the establishment of experimental animal model of burns, the
use of SD (Sprague- Dawley) rats after anesthesia 90℃ hot water burns 10
seconds and 15 seconds, divided into two groups after the burn, all in 24 hours
and 48 hours after the implementation of femoral vein intubation surgery to
collect blood, detection of its lipid peroxidation - malondialdehyde (MDA) of
the change. The results showed that in 10 seconds of burn time 48 hours after
serum MDA increased significantly under this condition confirmed its
significant oxidative damage.
Further experiments using methanol extract of Rhodiola applied in the SD
rat burn affected area, twice daily, taken 48 hours after the blood and skin tissue
and to detect the TNF-a IL-1β and ORAC and MDA, the present experimental
results to burn for 48 hours after administration, serum ORAC and MDA values
were significantly improved, while detection of the inflammation of the skin
index (TNF-a IL-1β) were significantly reduced. Conclusion: The results that
methanol extract of Rhodiola rosea may be reduced by the increase in lipid
peroxidation and total antioxidant power in vivo and reduce inflammation of the
skin factor (TNF-a IL-1β) to achieve after burns victims caused by the
protective effect.
目錄
摘要 1
ABSTRACT 7
目錄 10
第一章 序論 13
1-1前言與研究背景 13
第二章 文獻回顧 15
2-1 皮膚構造之簡介 15
2-2燒燙傷簡介 18
2-2-1 燒燙傷類型和臨床症狀 18
2-2-2 燒燙傷的嚴重程度 19
2-2-3 燒燙傷深度分級 20
2-2-4 燒燙傷體表面積百分比的評估方法 22
2-2-5 燒燙傷的臨床處理方式 23
2-2-6 自由基簡介 26
2-2-7 發炎反應 27
2-3. 燒燙傷動物模式之文獻探討 28
2-3-1 燒燙傷動物實驗模式 28
2-3-2 燒燙傷的氧化及發炎反應 30
2-3-3 氧化性傷害之生物指標-丙二醛 31
2-3-4 總抗氧化能力之分析 33
2-3-5 病理組織切片- 蘇木紫與伊紅染色法 ( Hematoxylin and Eosin stain ) 34
2-4. 紅景天之簡介 35
2-5. 紅景天的藥理特性及相關研究之探討 37
2-6 研究動機及目的 39
第三章 研究材料與方法 41
3-1 實驗動物 41
3-2 紅景天萃取製備 41
3-3 實驗設計 42
3-3-1 製備大鼠燒燙傷模型 45
3-3-2 紅景天及抗氧化藥物處理 45
3-3-3 組織切片前處理 46
3-3-4 皮膚樣本之前處理 46
3-4. 檢測方法 47
3-4-1 ORAC分析方法 47
3-4-2 MDA之測定 49
3-4-3 發炎指數TNF‐α、IL‐1β之測定 51
3-5. 統計方法 54
第四章 結果與討論 55
4-1 結果 55
4-1-1 不同燙傷時間下血清中氧化性壓力指標變化結果 55
4-1-2 給藥後血清中氧化性壓力指標檢測結果 57
4-1-3 血清中總抗氧化力檢測結果 59
4-1-4 皮膚總抗氧化力檢測結果 61
4-1-5 皮膚中發炎指標IL-1β檢測結果 63
4-1-6 皮膚中發炎指標TNF‐α檢測結果 65
4-1-7 皮膚病理組織切片檢查結果 67
4-2 討論 76
參考文獻 79
圖目錄
圖一 皮膚結構圖 16
圖二 表皮層和真皮層結構圖 17
圖三 一度燙傷 20
圖四 二度燙傷 21
圖五 三度燙傷 22
圖六 成人九法則 23
圖七 脂質過氧化脂連鎖反應流程圖 32
圖八 MDA 結構圖 33
圖九 薔薇紅景天 36
圖十 紅景天苷Salidroside及酪胺醇ρ-tyrosol 結構圖 37
圖十二 實驗(2) 檢測紅景天抗發炎之效果 44
圖十三 不同燙傷條件下血清中MDA值 56
圖十四 血清中MDA值 58
圖十五 血清中總抗氧化力( ORAC )值 60
圖十六 皮膚組織中總抗氧化力( ORAC )值 62
圖十七 皮膚中發炎指數指標細胞介白素一( IL-1β )值 64
圖十八 皮膚中發炎指數指標腫瘤壞死因子( TNF-?)值 66
圖十九 在100倍光學顯微鏡下控制組(control)皮膚組織 68
圖二十 在400倍光學顯微鏡下控制組(control)皮膚組織 69
圖二十一 在100倍光學顯微鏡下燙傷後空白給藥組(vehicle)皮膚組織 70
圖二十二 在400倍光學顯微鏡下燙傷後空白給藥組(vehicle)皮膚組織 71
圖二十三 在100倍光學顯微鏡低劑量紅景天給藥組(100mg)皮膚組織 72
圖二十四 在400倍燙傷後低劑量紅景天給藥組(R100mg )皮膚組織 73
圖二十五 在100倍光學顯微鏡下高劑量紅景天給藥組(R+ 250mg )皮膚組織 74
圖二十六 在400倍光學顯微鏡下高劑量紅景天給藥組(R+250mg)皮膚組織 75
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