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研究生:李子聰
研究生(外文):Tsz-Chung
論文名稱:利用蛋雞動物模式研究中草藥抗氧化作用的功效
論文名稱(外文):Investigation on antioxidant effect of chinese herbal medicine by chicken model
指導教授:高紹軒
指導教授(外文):Shau-Hsuan Kao
學位類別:碩士
校院名稱:中山醫學大學
系所名稱:生化暨生物科技研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:46
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七層塔 (Occimum Grastissimum L.; OGL)是台灣的常用民間保健草藥之一,具有許多抗氧化的成分,根據中草藥的文獻指出七層塔有治療咽喉腫痛、咳血及肝炎等療效。先前在本實驗室的研究中,用高粱酒粕餵飼蛋雞,利用Thiobarbituric acid reative substances (TBARS) 作為一脂質過氧化的指標,測定蛋黃脂質氧化的情形。結果發現餵飼蛋雞一般飼料含15%及30%高梁酒粕蛋黃會有脂質過氧化的情形。因此本實驗想利用高粱酒粕餵飼蛋雞建立氧化緊迫的動物模式,再添加中草藥七層塔入飼料中,研究中草藥抗氧化作用的功效。32隻蛋雞採任食方式,分為4組,A組為對照組餵食一般飼料, B組餵食一般飼料料添加30%高梁酒粕,C、D組餵食一般飼料含30%高梁酒粕並分別添加0.01%及0.05%七層塔。結果顯示蛋雞任食一般飼料添加30%高梁酒粕後,所產雞蛋中蛋黃MDA含量為141.6 nmol/50μl egg yolk,高於正常對照A組的61.9 nmol/50μl egg yolk,經統計發現 (P<0.01)有極顯著差異,與本實驗室先前研究結果相符。而C、D組蛋黃MDA含量分別為136.1 nmol/50μl egg yolk及127.6 nmol/50μl egg yolk都較B組低,有除去過氧化酵素的現象,但統計上並無顯著差異。綜合以上結果,我們認為在蛋雞的飼料中添加適當比例的高梁酒粕能夠建立氧化緊迫現象的動物模式,而此模式未來可作為應用於其它中草藥抗氧化或保肝功效的評估。

Our previous laboratory study investigated the oxidative stress of dietary sorghum distillery residue in chicken animal model by measuring the level of TBARS (thiobarbituric acid reactive substance) in egg yolk. The results of this study showed that feeding diets of chicken with 15% and 30% sorghum distillery residue can induce the increase of f TBARS level in egg yolk. In this study,we want to use this animal modol to investigate the antioxidant effect of chinese herbal medicine (Occimum Grastissimum L.; OGL) supplementation. Thirty-two chickens were randomly divied into 4 groups (n=8), the control group (A group) was fed with basal diet, B groups were fed with basal diet and 30% sorghum distillery residue and other groups (C and D groups) were fed with basal diet and 30% sorghum distillery residue with 0.01% or 0.05% OGL. respectively. The result showed that malondialdehyde (MDA) values in B group (141.6 nmol/50μl egg yolk ) were significantly higher than control group (61.9 nmol/50μl egg yolk), this is in accordance with the results of a previous study. MDA values in C group (136.1 nmol/50μl egg yolk) and D group (127.6 nmol/50μl egg yolk) were lower than B group, but no significantly difference. In conclusion, the sorghum distillery residue induced-oxidative stress in chicken can apply to investigate the antioxidant effect of other chinese herbal medicine in the future.

壹、中文摘要……………………………………………………………4
貳、英文摘要……………………………………………………………6
參、前言…………………………………………………………………8
一、脂肪酸與雞蛋保存的關係
二、生物體氧化傷害與抗氧化防禦系統
(一)活性氧定義
(二)自由基與活性氧產生途徑
(三)自由基及活性氧對生物體分子之傷害
(四)人體的抗氧化防禦系統
三、酚類化合物及其抗氧化性
四、中草藥對飼料應用之進展
五、七層塔
肆、實驗動機…………………………………………………………17
伍、實驗方法與材料…………………………………………………19
陸、實驗結果…………………………………………………………21
柒、討論………………………………………………………………24
捌、參考文獻…………………………………………………………27
玖、圖表………………………………………………………………36


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