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研究生:洪筱婷
研究生(外文):Hsiao-Ting
論文名稱:探討香瓜茄對於老化與長期飲食果糖誘發代謝症候群之研究
論文名稱(外文):Study of Solanum Muricatum regarding the aged and the Long-term Dietary Fructose-induced Metabolic Syndrome
指導教授:張菡馨
指導教授(外文):Han-Hsin Chang
學位類別:碩士
校院名稱:中山醫學大學
系所名稱:營養學研究所
學門:醫藥衛生學門
學類:營養學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:121
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果糖消耗量增加所伴隨的後續效應日益嚴重,由於代謝症候群的高危險性和致死率是目前己開發國家主要的健康危機,然而著眼於過去文獻有關果糖誘發代謝症候群中相關危險因子之研究,發現大多是著重於短時間介入高劑量果糖且只針對單一症狀探討。因此,本研究將分為三大部分(1) 觀察小鼠不同週齡之生理代謝變化;(2) 20%果糖水對於動態生理變化之衝擊效應;(3) 以20%果糖飲水建立代謝症候群之動物模式,共介入21週,同時整合性探討生理代謝、氧化壓力變化、致病機轉及探討香瓜茄是否能有效降低代謝症候群之危險因子的評估分析,並觀察組織形態和功能上的病理改變,有利於縱深研究其對代謝器官之衝擊效應。結果顯示伴隨小鼠週齡增加則使其體重增加、血脂上升及抗氧化酵素活性被削弱之情形(p<0.01)。飲食中添加果糖導致生理代謝方面出現異常,同時在體重、血壓、血糖、總膽固醇皆有顯著上升(p<0.01),且在腎功能方面,肌酸酐值與尿酸值亦顯著增加(p<0.01);而氧化壓力方面則造成肝及腎臟其脂質過氧化程度顯著增加(p<0.01),以及穀胱甘肽(glutathione;GSH)與觸酶(catalase;CAT) 的抗氧化酵素活性顯著下降(p<0.01);在組織病理學方面顯示肝、腎組織有明顯脂肪堆積情形,且鮑氏囊有顯著紅血球浸潤情形。而經過香瓜茄治療後,其體重有顯著下降(p<0.01),同時血中尿素氮和肌酸酐的含量也降低(p<0.01),除此之外,也提升體內觸酶的活性(p<0.01),但在血脂、肝功能及脂質過氧化方面卻未能達到改善,甚至有出現惡化的趨勢發生。根據本研究發現老化過程中,可能會引起一連串的代謝器官功能衰退效應產生,更進一步了解的是老化將會受到相關因子刺激而加速其進展之過程。另外,飲食中攝入果糖可能會導致肥胖、血壓上升及血脂代謝異常的現象發生,同時合併組織脂質過氧化程度增加,伴隨著體內抗氧化酵素活性出現削弱的趨勢,在肝臟、腎臟皆有觀察到脂肪堆積的情形。因此,我們認為飲食中攝入果糖所帶來的衝擊效應將更值得被關注,一方面是希望未來能訂定飲食中果糖的安全使用劑量;另一方面則是建立改善代謝症候群之有效健康食品,將提倡響應未來養生保健的趨勢發展。

Increasing consumption of fructose is getting more serious nowadays. Due to the high risk of metabolic syndrome (MS) and lethal rate is the major crisis in developed countries at present. Focus on previous studies associated with the risk factors for MS induced by fructose purposed to use high dose in short-term and to discuss only single symptom, moreover, they didn’t pay attention on overall condition. Therefore, this study will divide three parts. (1) To observe physiological metabolic changing in different stages compared with the weeks for mice, (2) to observe the detrimental effect of 20% fructose dietary drinking water on physiological metabolic changing in different stages compared with the weeks for mice, (3) Set up animal model of MS by 20% dietary drinking fructose water, intervened for completely 21 weeks, at the same time, we discuss physiological metabolism, variation of oxidative stress, analysis S. muricatum whether effectively reduce risk factors or not, furthermore, observe the morphological, histological and functional changing in pathology to reveal underlying mechanism, which benefited the deeply research in the metabolic organs. The results showed that weight, blood lipid increased and anti-oxidative enzyme activity decreased, mice age increasing (p&lt;0.01). Dietary fructose consumption increasing lead to abnormally physiological metabolism, all of the weight, blood pressure, fasting blood sugar and total cholesterol have notable rising (p&lt;0.01). However, damage to liver and kidneys of lipid peroxidation level significant increased (p&lt;0.01) and the anti-oxidative activity of glutathione (GSH) and catalase (CAT) was significantly decreased (p&lt;0.01). Fat obviously accumulated in liver and kidneys and the blood red cells infiltrated in Bowman’s capsule in histopathology. After treatment by S. muricatum, body weight obviously decreased (p&lt;0.01), besides, the blood urea nitrogen (BUN) and creatinine (CRE) in blood significantly decreased (p&lt;0.01), in addition to, the activity of CAT promoted (p&lt;0.01). However, blood lipid, liver function and lipid peroxidation level do not significant improve. According to this study, we found that fructose could lead to obesity, unusual of blood lipid metabolism and raise blood pressure. Increasing lipid peroxidation level in tissues accompanied with the weaken activity of anti-oxidation activity, lipid accumulating in liver and kidneys is obviously. As a result, we think that detrimental effect of fructose intake should be noticed. Setting up safe dose in dietary fructose is the most important thing in the future. In another part, establishing the effective healthy food to improve MS and promoting to keep in good health and care in the future.

目錄
口試委員會審定書
論文電子檔案上網授權書
誌謝--------------------------------------------------------------------------------------------i
目錄--------------------------------------------------------------------------------------------ii
圖次--------------------------------------------------------------------------------------------vii
表次--------------------------------------------------------------------------------------------xi
摘要
  中文摘要--------------------------------------------------------------------------------xii
  英文摘要--------------------------------------------------------------------------------xiv
第一章 緒論----------------------------------------------------------------------------------1
第二章 文獻探討
第一節 老化
(一) 老化定義與老化理論---------------------------------------------------------4
(二) 老化與氧化損傷之因果關係------------------------------------------------7
第二節 代謝症候群
(一) 代謝症候群定義及診斷標準-------------------------------------------------9
(二) 代謝症候群之危險因子與疾病之相關性---------------------------------11
第三節 果糖
一、 飲食中果糖所扮演之角色----------------------------------------------------13
二、 縱觀飲食中果糖與代謝症候群之相關性----------------------------------15
三、 果糖對肝臟代謝所產生之衝擊效應----------------------------------------17
四、 果糖對腎臟之慢性損傷-------------------------------------------------------20
五、 探討果糖造成抗氧化酵素防禦系統失衡之影響-------------------------22
第四節 香瓜茄
(一) 背景-------------------------------------------------------------------------------24
(二) 香瓜茄之成份介紹-------------------------------------------------------------25
第五節 抗氧化酵素防禦系統
(一) 概論-------------------------------------------------------------------------------26
(二) 脂質過氧化 (lipid peroxidation) --------------------------------------------27
(三) 榖胱甘肽 (GSH) 氧化還原系統--------------------------------------------28
(四) 觸酶 (CAT) --------------------------------------------------------------------30
第三章 研究目的----------------------------------------------------------------------------31
第四章 材料與方法
第一節 實驗藥品------------------------------------------------------------------33
第二節 儀器設備------------------------------------------------------------------35
第三節 研究方法
(一) 實驗動物來源-------------------------------------------------------------------37
(二) 實驗動物飼養及分組配方---------------------------------------------------37
(三) 果糖水製備與香瓜茄濃縮乾燥製備---------------------------------------40
(四) 小鼠基礎生理代謝變化紀錄與臟器比重---------------------------------41
(五) 血清生化檢驗分析------------------------------------------------------------42
(六) 血清胰島素含量測定---------------------------------------------------------43
(七) 脂質過氧化程度分析---------------------------------------------------------44
(八) 總榖胱甘肽含量測定---------------------------------------------------------46
(九) 榖胱甘肽還原酶活性測定---------------------------------------------------48
(十) 榖胱甘肽過氧化酶活性測定------------------------------------------------49
(十一) 觸酶活性測定----------------------------------------------------------------51
(十二) 蛋白質測定-------------------------------------------------------------------52
(十三) 組織病理形態分析----------------------------------------------------------53
(十四) 組織冷凍切片分析----------------------------------------------------------54
(十五) 統計分析----------------------------------------------------------------------55
第五章 結果
一、 針對小鼠不同週齡其生理代謝器官功能衰退變化
(一) 犧牲點之體重變化與肝、腎臟器之比重--------------------56
(二) 血清生化檢驗分析------------------------------------------------59
(三) 抗氧化酵素活性分析---------------------------------------------61
(四) 組織病理切片染色分析------------------------------------------63
二、 觀察經過20%果糖水介入期間小鼠之動態生理壓力變化
(一) 20%果糖水介入期間之攝食代謝紀錄--------------------------65
(二) 20%果糖水介入期間之血壓、體重及肝、腎臟器比重-----67
(三) 20%果糖水介入期間之血清生化檢驗與抗氧化酵素活性分析
-------------------------------------------------------------------------70
(四) 20%果糖水介入期間之肝、腎、胰臟病理及冷凍切片染色分析-------------------------------------------------------------------------76
三、 小鼠模式之果糖誘發代謝症候群以香瓜茄作為嘗試性治療
(一) 連續餵食20%果糖水21週其攝食、飲水、糞便與尿液量之變
化----------------------------------------------------------------------80
(二) 連續餵食20%果糖水21週其平均熱量攝取量、體重及血壓之變化-------------------------------------------------------------------81
(三) 連續餵食20%果糖水21週後並經由20%香瓜茄治療6週其平均果糖攝取所占平均熱量百分比、犧牲點體重及臟器比重之變化-------------------------------------------------------------------83
(四) 連續餵食20%果糖水21週後並經由20%香瓜茄治療6週其血清化檢驗-------------------------------------------------------------86
(五) 連續餵食20%果糖水21週後並經由20%香瓜茄治療6週其脂質過氧化程度分析與抗氧化酵素防禦系統活性分析-------88
(六) 連續餵食20%果糖水21週後並經由20%香瓜茄治療6週其肝、腎、胰臟之組織病理切片染色比較----------------------90
四、 討論 ------------------------------------------------------------------------------------92
(一) 探討老化機制及相關因子之衝擊效應
(二) 探討果糖基礎生理代謝之衝擊與肝臟、腎臟調節系統之紊亂
(三) 整合性探討香瓜茄調節生理代謝與改善氧化損傷之功效
五、 結論-------------------------------------------------------------------------------------102
六、 參考文獻-------------------------------------------------------------------------------103
(附錄一) 國際功能性食品研討會摘要-------------------------------------------------119
(附錄二) 第三十八次台灣食品科技年會摘要----------------------------------------120




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