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論文名稱(外文):Effect of strontium on physicochemical properties and biocompatibility of calcium silicate bone grafts
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鈣矽骨水泥被視為是一個具有潛力的生醫骨填補物,而鍶是人體內的一個微量元素,常被用於治療骨質疏鬆症,主要是因為鍶能誘導成骨細胞活性,刺激骨生成,並且能夠抑制噬骨細胞減少骨吸收,故在本研究中,主要是評估添加鍶之鈣矽骨水泥其物理化學性質以及生物相容性。利用sol-gel的方式來製備含鍶之鈣矽粉體,鍶含量為鍶鈣的莫爾比Sr/(Sr+Ca) ,含量分別為0%、1%、5%以及10%。主要分析方式有晶相分析(XRD)、微結構分析(SEM)、對徑拉伸強度測試(DTS)及硬化時間測試(setting time)。
在結果方面,各組的粉體顆粒大小約在1-5μm。在XRD結果可以觀察到粉體的結晶相在繞射峰2θ = 32°到34°之間,其峰值明顯較大,推測是由於β-Ca2SiO4相,在藉由離子交換後,部分的鈣被鍶所取代,且由於鍶的離子半徑(1.13Å)比鈣(1.00Å)大,在鍶併入鈣矽陶瓷中之後,可能導致峰值的變寬。在混合水之後,可以觀察到結晶相的繞射峰2θ = 29.3°有鈣矽水合(calcium silicate hydrate)的峰值。硬化時間方面,添加10%鍶的硬化時間也從控制組的22分鐘減少到了17分鐘。至於強度結果則並沒有明顯的改變,範圍在2.0~2.4 MPa。因此,包含鍶的鈣矽骨水泥有可能是具有潛力的鈣矽骨填補物。

Calcium silicate cements (CSC) have been regarded as a potential biomaterial for bone graft substitutes. Strontium (Sr) is a trace element in human body and been used for treatment of osteoporosis. Sr is found to induce osteoblast activity by stimulating bone formation and to reduce bone resorption by restraining osteoclasts. The aim of this study was to evaluate the physicochemical properties of Sr-containing CSC. The calcium silicate with equimolar ratio of Ca to Si was used as the control. Various amounts of Sr with a molar ratio of 1%, 5% and 10% for Sr/(Sr + Ca) were added to sol-gel precursors. The major techniques used for characterizing the various specimens included X-ray diffraction (XRD), scanning electron microscopy (SEM), diametral tensile strength and setting time.
As a result, the particle size of calcium silicate ceramics powder ranged from 1 μm to 5 μm. The XRD patterns shows that major diffraction peaks of the four powders were at 2θ between 32 and 34o attributed to β-dicalcium silicate (β-Ca2SiO4) phase. Although the Ca was replaced in part by Sr through ionic exchange, the incorporation of Sr to calcium silicate ceramics may lead to a broadening peak. This can be explained by the fact that the radius of Sr ions (1.13Å) is larger than that of Ca ions (1.00Å), namely, the microstrain effect. After mixing with water, XRD patterns of all cements revealed an obvious diffraction peak around 2θ = 29.3o, corresponding to the calcium silicate hydrate gel, and incompletely reacted inorganic component phases of β-Ca2SiO4. 10% Sr significantly shortened setting time from 22 min for the CSC control to 17 min. The addition of Sr did not affect the strength ranged from 2.0 to 2.4 MPa as these specimens were not significantly different (p > 0.05) from control groups (2.5 MPa). Our findings indicated that Sr-CSC may be a candidate for bone repair.

致謝 I
中文摘要 II
Abstract III
目錄 IV
表目錄 VI
圖目錄 VII
第一章 文獻回顧 1
1-1 生醫材料 1
1-2 生醫材料在硬組織上的應用 2
1-3 骨水泥 4
1-3-1 生物活性玻璃骨水泥 4
1-3-2 鈣磷骨水泥 5
1-3-3 天然高分子骨水泥 5
1-4 鈣矽骨水泥 6
1-5 微量添加物-鍶 6
1-6 高分子-明膠 9
1-7 溶膠凝膠法 9
1-8 研究動機與目的 10
第二章 材料與方法 11
2-1 生醫陶瓷粉體的製備 11
2-2 骨水泥的製備 12
2-3 浸泡實驗 12
2-4 材料特性分析 12
2-4-1 粉體熱重-熱示差分析 12
2-4-2 XRD分析 12
2-4-3 掃描式電子顯微鏡觀察 13
2-4-4 穿透式電子顯微鏡觀察分析 13
2-4-5 骨水泥徑向拉伸強度測試 13
2-4-6 骨水泥硬化時間之測試 14
2-4-7 浸泡後pH值之變化 14
第三章 結果與討論 15
3-1 粉體分析 15
3-1-1 熱重-熱示差分析 15
3-1-2 XRD晶相分析 17
3-1-3 顯微結構分析 19
3-1-4 穿透式電子顯微鏡顯微結構分析 22
3-2 骨水泥分析 23
3-2-1 XRD晶相分析 23
3-2-2 顯微結構分析 25
3-2-3 骨水泥對徑拉伸強度測試 29
3-2-4 骨水泥硬化時間測試 31
3-3 生物活性分析 33
3-3-1 骨水泥浸泡後顯微結構分析 33
3-3-2 骨水泥浸泡後晶相分析 40
3-3-3 骨水泥浸泡後對徑拉伸強度測試 45
3-3-4 浸泡後pH值變化 48
3-3-5 浸泡後離子釋放濃度 50
3-4 生物相容性分析 54
3-4-1 細胞增生 54
3-4-2 細胞型態 55
3-4-3 鈣離子染色 58
第四章 結論 61
第五章 參考文獻 63

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