臺灣博碩士論文加值系統

本論文研究生物相容性高分子之合成與其在手術防沾黏薄膜以及結合螢光高分子進行即時螢光訊號量測之應用。合成一系列具生物相容性、生物可分解性之高分子作為防沾粘膜，防止手術後傷口沾黏。將生物相容性高分子單體和螢光高分子混合物經由噴印及光聚合反應在薄膜上製作螢光訊號標記點。將薄膜植入大鼠體內進行動物試驗。本研究開發出可於植入期間，用體外即時影像觀測系統以非侵入方式偵測螢光訊號來觀測薄膜在體內之降解情況的技術，結果與動物試驗解剖大鼠觀察降解與沾黏情形吻合。調整高分子之分子結構、不同親疏水端成分與不同親疏水端比例，並以氧電漿處理，進行薄膜表面改質，再以TGA、DSC、螢光顯微鏡、ESCA等儀器分析各系列薄膜之特性，探討高分子材料結構及表面改質對薄膜表面特性及防沾粘性之影響。

The synthesis of biocompatible polymers and their applications for postoperative anti-adhesion tissue, together with in-vivo imaging of their degradation behaviors were studied. The synthesis of a series of biocompatible and biodegradable polymers was used as anti-adhesion barriers to prevent wound adhesion after surgery. The mixtures of biocompatible monomer and fluorescent polymer were printed and photopolymerized to make fluorescent markers on the film. The films were implanted to carry out animal experiments in rats. In-vivo degradation behaviors of the surface-marked films were investigated non-invasively by monitoring the location of fluorescent signal. The degradation behaviors of various films observed in the animal study were consistent with those observe by in-vivo imaging. The films were modified by changing the molecular structure of polymers, the ratio of hydrophilic/hydrophobic segments, and applying oxygen plasma treatment. The effects of chemical structure and surface modification on the surface properties and anti-adhesion performance were investigated.

中文摘要…………………………………i
英文摘要…………………………………ii
目錄…………………………………… iii
圖目錄……………………………………………………vi
表目錄……………………………………………………xi
第一章 緒論……………………………………………………1
§ 1-1 前言……………………………………………………1
§ 1-2 研究目的與動機…………………………………………………1
第二章 文獻回顧……………………………………………………4
§ 2.1 生物相容性/可分解高分子……………………………4
§ 2.2 生物可分解性團聯共聚合高分子…………………………6
§ 2.3 PEG-Poly Lactide/Caprolactone copolymer…………7
§ 2.4 高分支狀高分子…………………………………9
§ 2.5螢光標記…………………………………11
§ 2.6電漿活化之表面改質處理…………………………………13
第三章 實驗部分…………………………………14
§ 3.1 實驗藥品…………………………………14
§ 3.2 實驗儀器…………………………………18
§ 3.3 實驗方法…………………………………19
§ 3.3.1 共聚合高分子製備……………………………19
§ 3.3.2 薄膜製作……………………………………………………23
§ 3.3.3 噴印點膠螢光共軛高分子於薄膜上……………24
§ 3.3.4 動物體內試驗…………………………………25
§ 3.3.5 In vivo imaging…………………………………25
§ 3.3.6 氧電漿活化之表面改質處理……………………27
第四章 結果與討論……………………………………………………28
§ 4.1 G26LAn/PEMLn系列薄膜………………………………28
§ 4.1.1 G26LAn/PEMLn成膜形28
§ 4.1.2 PEMLn之傅立葉轉換紅外線光譜分析……………34
§ 4.1.3 G26LAn/PEMLn生物毒性測試……………………36
§ 4.1.4 G26LAn/PEMLn之耐熱性質………………………37
§ 4.1.5 螢光共軛高分子之螢光標記點…………………….41
§ 4.1.6 G26LAn/PEMLn之IVIS觀測系統……………...…42
§ 4.1.7薄膜於老鼠體內手術後28天解剖觀察……………45
§ 4.2 PEMCn系列薄膜…………………………………...............47
§ 4.2.1 PEMCn系列……………………………………...….47
§ 4.2.2 PEMCn之傅立葉轉換紅外線光譜分析…….…...….50
§ 4.2.3 PEMCn生物毒性測試……………………………….51
§ 4.2.4 PEMCn之熱分析…………………………………….52
§ 4.2.3 螢光標記點於薄膜之附著力測試. ……………......54
§ 4.2.4 PEMCn系列薄膜表面特性研究…………………….56
§ 4.2.5 PEMCn系列之IVIS觀測系統………………………60
§ 4.2.6 PEMCn系列薄膜於手術後28天解剖觀察…………62
§ 4.3 GCLnIx系列薄膜…………………………………...............64
§ 4.3.1 GCLnIx系列薄膜的結構與反應式…………………64
§ 4.3.2 GCLnIx之傅立葉轉換紅外線光譜分析……………67
§ 4.3.3 GCLnIx成膜情形…………………………………....69
§ 4.3.4 GCLnIx之熱分析……………………………………69
§ 4.3.5 氧電將之活化表面改質處理….……………………76
§ 4.3.6 GCLnIx系列之IVIS觀測系統………………………84
§ 4.3.7 GCLnIx系列薄膜於手術後28天解剖觀察………..87
第五章 結論…..…….…….……….…………………..………….……89
§ 5.1 G26LAn/ PEMLn系列薄膜…………………………………89
§ 5.2 PEMCn系列薄膜…………………………………………90
§ 5.3 GCLnIx系列薄膜…………………………………………90
第六章 參考文獻……………………………………………………91

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