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研究生:謝佳容
研究生(外文):chia-jung hsieh
論文名稱:建立病毒誘導之腫瘤細胞模式並觀察維生素C對此腫瘤細胞模式之作用
論文名稱(外文):Establish a virus-induced tumor cell model and study the effects of vitamin C on this cell model
指導教授:魏秋偉魏秋偉引用關係余永倫
指導教授(外文):Chyou-Wei WeiYung-Luen Yu
學位類別:碩士
校院名稱:弘光科技大學
系所名稱:營養醫學研究所
學門:醫藥衛生學門
學類:營養學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:88
中文關鍵詞:日本腦炎病毒維生素C腫瘤
外文關鍵詞:Japanese encephalitis virusvitamin Ctumor
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日本腦炎病毒 (JEV) 屬於黃質病毒科黃質病毒屬。日本腦炎病毒的非結構性蛋白 NS3 具有絲胺酸蛋白酶(serine protease)、 RNA水解螺旋酶(helicase)活性,是複製過程中扮演著重要的角色。在之前的研究中已證實隸屬同科的 C 型肝炎病毒 (HCV) NS3 蛋白具有誘導 NIH3T3 細胞轉型之能力。 JEV NS3 和 HCV NS3 具有高度的相似性, 因此我們推測 JEV NS3 也具有誘導細胞轉型的能力。本實驗將全段 JEV NS3 基因以及 NS3 絲胺酸蛋白酶基因分別接至自發螢光蛋白表現載體 pEGFP-C3 ,轉染 NIH3T3 細胞後,表現NS3 螢光融合蛋白,以 G418 篩選培養,初步結果證實了 NS3 及NS3 絲胺酸蛋白酶都可讓細胞轉型為腫瘤細胞,因此我們第一個成功建立了 JEV 誘導瘤形成之細胞模式。
維生素 C 是水溶性抗氧化物質,之前有文獻指出,維生素 C可抑制腫瘤細胞生長,並且會清除自由基。因此,利用我們建立的這個腫瘤細胞模式,來檢測維生素C對病毒誘導的腫瘤細胞之抑制作用。綜合此篇論文的研究,我們是第一個成功建立了 JEV 誘導腫瘤形成的細胞模式,可提供此細胞模式供相關研究使用;同時,我們也證實維生素 C 可以抑制病毒誘導的腫瘤細胞生長。
Japanese encephalitis virus ( JEV) and Hepatic C virus (HCV) belong to Flaviviridae Flavivirus. Their non-structure protein 3 (NS3) has high homologues. NS3 contains protease domain and helicase domain. Previous studies have been demonstrated that HCV NS3 and HCV NS3 protease domain (NS3P) can induce NIH3T3 cells to transform into tumor cells. Therefore we suggest that JEV NS3 and NS3P can also induce cell to transform into tumor cells. In this study, we successfully construct pEGFP NS3 and pEGFP NS3P. After these plasmids transfection into NIH3T3 cells and selection by treatment G418 about 5-6 weeks, the NIH3T3 cells lose contact inhibition and transform into tumor cells. In this study, we firstly establish a JEV NS3- and NS3P-induced tumor cell model. In addition, using this model, the anti-tumor effect of vitamin C on this cell model has been studied. Our data shows that vitamin C can inhibit cell growth on JEV NS3- and JEV NS3P-induced transformed cells. Overall, we successful provide a JEV NS3- and NS3P-induced tumor cell model and demonstrate that vitamin C can inhibit cell growth on virus-induced tumor cell model.
目錄………………………………………………………………………….2
縮寫表……………………………………………………………………….4
中文摘要…………………………………………………………………….5
英文摘要…………………………………………………………………….6
第一章、緒論………………………………………………………………..7
第二章、文獻回顧…………………………………………………………..9
2.1.1日本腦炎與病毒之發現....................................................................9
2.1.2日本腦炎病毒的特性…………………………………………….......10
2.1.3日本腦炎病毒NS3蛋白之功能………………………………………13
2.2自由基與疾病之間的相關性………………………………………….15
2.3自由基對生物體之氧化傷害………………………………………….15
2.4自由基定義…………………………………………………………….17
2.4.1超氧自由基…………………………………………………………..18
2.4.2過氧化氫……………………………………………………………..19
2.5生物體內抗氧化之防禦系統………………………………………….19
2.5.1酵素性抗氧化防禦系統……………………………………………..20
2.5.2非酵素性抗氧化防禦系統…………………………………………..21
第三章、實驗目的………………………………………………………...23
第四章、材料與方法……………………………………………………….24
第五章、結果……………………………………………………………….38
第六章、討論……………………………………………………………….46
第七章、結論……………………………………………………………….54
第八章、參考文獻…………………………………………………………55
圖…………………………………………………………………………...66
附錄………………………………………………………………………...88
第八章 參考文獻
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