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研究生:郭珮怡
研究生(外文):Pei I Kuo
論文名稱:以火焰式原子吸收光譜法配合原子捕捉器測定台灣青草沖泡液中元素
論文名稱(外文):Determination of trace elements in Taiwan herbs infusions by flame atomic absorption spectrometry coupled with STAT
指導教授:許振原
指導教授(外文):Jenn Yuan Shen
學位類別:碩士
校院名稱:中華醫事科技大學
系所名稱:生物科技研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2009
畢業學年度:96
語文別:中文
論文頁數:87
中文關鍵詞:元素火焰式原子吸收光譜法原子捕捉器
外文關鍵詞:taiwanflame atomic absorption spectrometryinfusions
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在全球各地廣泛的使用有療效性的植物及其製劑(包含冷或熱的沖泡液)。亞洲國家在使用草藥時,通常是在植物自然狀態下使用,像乾燥的花草和根莖。毒性元素會污染這些植物,這牽涉到許多複雜的潛在因素,如品種、收割與處理過程、污染物濃度和暴露的時間、地形、地質及儲存方式。本研究藉由火焰式原子吸收光譜法(Flame Atomic Absortion Spectrophotometry)和原子捕捉器-火焰原子吸收光譜法(Slotted Tube Atom Trap - Flame Atomic Absorption Spectrometry, STAT-FAAS),測定台灣台南市所販售的九種草藥中元素濃度。藉由原子捕捉器提升火焰式原子吸收光譜法的靈敏度。在樣品的處理上使用草藥的沖泡液,以超純硝酸做乾式加熱方法消化。研究中測定7種元素,包含:鐵、銅、鋅、錳、鎘、鎳、鉛。結果顯示在草藥沖泡液中測的銅 0.2-2.475 mg/kg,鐵 4.875-60.45 mg/g,錳0.475-19.8 mg/kg,鋅1.125-18.9 mg/kg。鎘、鎳、鉛其所測得的數值低於本實驗的偵測極限。本實驗之偵測極限分別為:鎳:0.052 ppm,鎘 :0.010 ppm,鉛:0.081 ppm。本研究中,樣品沖泡液的製備如一般民眾飲用草藥前的處理方法。然而,這些草藥材很容易受到污染,包括種植和加工過程。期望本實驗能夠提供一個快速的檢測方式來測定草藥中的微量元素濃度。
Medicinal herbs and their preparations (hot and cold infusions) are widely used by human beings all over the world. Asian countries use herbal remedies more in their natural states, like dried herbs and roots. The potential contamination of raw herbal products with toxic elements depends on many complex factors like species, cultivation, processing, harvesting time, level and duration of contaminant exposure, topography, geographical origin, storage. The determinations were performed using flame atomic absorption spectrometry (FAAS ) and slotted tube atom trap - flame atomic absorption spectrometry (STAT-FAAS ). Sensitivity enhancement by using atom trapping in flame atomic absorption spectrophotometry was examined for increasing the residence time of the analyte atoms in the light path. The infusion samples were digested by superpure nitric acid. In this present, the following elements were determined in the leaf infusion : Fe, Cu, Zn, Mn, Cd, Ni and Pb. The metal content of infusion samples were ranged from 0.2–2.475 mg/kg for Cu, 4.875 to 60.45 mg/g for Fe, 0.475 to 19.8 mg/kg for Mn, 1.125 to 18.9 mg/kg for Zn. Pb, Ni and Cd were not detected (limit of quantitation of Ni: 0.052 ppm, Cd :0.010 ppm, Pb: 0.081 ppm).For the sake of representing daily average uptake of elements, the infusion samples were prepared normally as tea. However, medicinal herbs may be contaminated easily during growing and processing. It is important to have a good quality control for herbal medicines in order to protect consumers from contamination.
第一章 研究背景與目的 - 1 -
第一節 台灣草藥飲用背景 - 1 -
第二節 微量元素之簡介 - 4 -
第三節 原子吸收光譜儀的簡介 - 8 -
第四節 火焰式原子吸收光譜儀儀器及原子捕捉器的簡介 - 10 -
第五節 研究目的 - 13 -
第二章 材料與研究方法 - 14 -
第一節 儀器設備 - 14 -
第二節 試劑 - 15 -
第三節 實驗器皿之清洗 - 15 -
第四節 溶液配製 - 16 -
第五節 中草藥樣品處理 - 21 -
2-5-1 樣品乾燥 - 21 -
2-5-2 青草沖泡液配置 - 21 -
2-5-3 樣品消化 - 22 -
第六節 方法驗證 - 23 -
2-6-1 檢量線 - 23 -
2-6-2 準確度 - 23 -
2-6-3 精密度 - 24 -
2-6-4 偵測極限 - 24 -
2-6-5 定量極限 - 24 -
2-6-6 污染控制 - 24 -
第三章 結果與討論 - 26 -
第一節 樣品前處理 - 26 -
3-1-1青草樣品-微波消化 - 26 -
3-1-2青草沖泡液-乾式加熱消化 - 26 -
第二節 最佳化條件 - 27 -
3-2-1 氣體流速 - 27 -
3-2-2 燃燒頭高度 - 27 -
第三節 分析方法驗證 - 28 -
3-3-1檢量線 - 28 -
3-3-2準確度 - 32 -
3-3-3精密度 - 34 -
3-3-4偵測極限及定量極限 - 36 -
第三節 分析方法應用 - 37 -
3-2-1 青草沖泡液及青草中元素含量測定 - 37 -
3-2-2 應用STAT-FAAS與FAAS測定元素之比較 - 39 -
3-2-3 青草沖泡液與青草中元素含量絕對值比較 - 40 -
第四章 結論 - 42 -
參考文獻 - 43 -
附錄. - 47 -
表一、微波消化系統針對九種草藥之最佳消化條件 - 48 -
表二、火燄式原子吸收光譜儀各元素最佳化條件 - 49 -
表三、肝炎草沖泡消化液添加回收率 - 50 -
表四、艾草沖泡消化液添加回收率 - 52 -
表五、七層塔沖泡消化液添加回收率 - 54 -
表六、肝炎草消化液添加回收率 - 56 -
表七、艾草消化液添加回收率 - 57 -
表八、七層塔消化液添加回收率 - 58 -
表九、以FAAS及STAT-FAAS測定鋅標準品精密度結果 - 59 -
表十、以FAAS及STAT-FAAS測定鎘標準品精密度結果 - 60 -
表十一、以FAAS及STAT-FAAS測定銅標準品精密度結果 - 61 -
表十二、以FAAS及STAT-FAAS測定錳標準品精密度結果 - 62 -
表十三、以FAAS及STAT-FAAS測定鎳標準品精密度結果 - 63 -
表十四、以FAAS及STAT-FAAS測定鐵標準品精密度結果 - 64 -
表十五、以FAAS及STAT-FAAS測定鉛標準品精密度結果 - 65 -
表十六、火燄式原子吸收光譜儀各元素之LOD、LOQ - 66 -
表十七 、火燄式原子吸收光譜儀測定草藥沖泡液元素含量 - 67 -
表十八 、火燄式原子吸收光譜儀測定草藥元素含量 - 68 -
表十九 、使用FAAS與STAT-FAAS測定草藥沖泡液元素含量 - 69 -
表二十 、火燄式原子吸收光譜儀測定草藥沖泡液、草藥消化液元素含量之百分比(%) - 70 -
表二十一、膳食營養素參考攝取量 - 71 -
表二十二、食品中一般常見重金屬含量的容許量 - 72 -
圖一、STAT接合FAAS火焰頭示意圖 - 73 -
圖二、以FAAS測定鋅標準品檢量線結果 - 74 -
圖三、以STAT-FAAS測定鋅標準品檢量線結果 - 75 -
圖四、以FAAS測定鎘標準品檢量線結果 - 76 -
圖五、以STAT-FAAS測定鎘標準品檢量線結果 - 77 -
圖六、以FAAS測定銅標準品檢量線結果 - 78 -
圖七、以STAT-FAAS測定銅標準品檢量線結果 - 79 -
圖八、以FAAS測定錳標準品檢量線結果 - 80 -
圖九、以STAT-FAAS測定錳標準品檢量線結果 - 81 -
圖十、以FAAS測定鎳標準品檢量線結果 - 82 -
圖十一、以STAT-FAAS測定鎳標準品檢量線結果 - 83 -
圖十二、以FAAS測定鐵標準品檢量線結果 - 84 -
圖十三、以STAT-FAAS測定鐵標準品檢量線結果 - 85 -
圖十四、以FAAS測定鉛標準品檢量線結果 - 86 -
圖十五、以FAAS-STAT測定鉛標準品檢量線結果 - 87 -
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