跳到主要內容

臺灣博碩士論文加值系統

(3.231.230.177) 您好!臺灣時間:2021/07/28 16:12
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:陳永軒
研究生(外文):Yung-Husan Chen
論文名稱:闊葉骨碎補、箭葉鳳尾蕨和雙花耳草之活性成分
論文名稱(外文):The Bioactive Constituents of Davallia solida, Pteris ensiformis and Hedyotis biflora
指導教授:吳永昌
指導教授(外文):Yang-Chang Wu
學位類別:博士
校院名稱:高雄醫學大學
系所名稱:天然藥物研究所
學門:醫藥衛生學門
學類:藥學學類
論文種類:學術論文
論文出版年:2008
畢業學年度:97
語文別:中文
論文頁數:277
中文關鍵詞:闊葉骨碎補箭葉鳳尾蕨雙花耳草
外文關鍵詞:Davallia solidaPteris ensiformisHedyotis biflora
相關次數:
  • 被引用被引用:0
  • 點閱點閱:452
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
從闊葉骨碎補中,藉由捕捉自由基之活性導引分離法,得到四個為新化合物,十個已知化合物,其中mangiferin及其衍生物具有最佳的抗氧化活性。闊葉骨碎補塊莖水抽出物,以不同溶液進行液液萃取,得到不同劃分,並分別含有不同比例之總多酚化合物含量;正丁醇劃分之總多酚化合物含量均大於各劃分,可能與正丁醇劃分之抗氧化力較佳有關,加上在正丁醇劃分中得到的十四個化合物皆為酚類化合物,其中有十一個化合物比維他命E有更強的自由基捕捉活性,亦與結果相呼應。
從箭葉鳳尾蕨水抽出物中分離得到三個新化合物及五個已知化合物。針對箭葉鳳尾蕨水萃取物進行組分之高效能液相層析 (HPLC) 分析,發現箭葉鳳尾蕨水萃取物中caffeic acid 和hispidin 衍生物有較高含量與抗氧化活性,對於箭葉鳳尾蕨水抽出物抗氧化力,多酚類化合物佔重要角色,尤其是 caffeic acid、hispidin 及 kaempferol 等衍生物。
並將上述具抗氧化化合物依照其抗氧化活性,歸納其結構與活性的關係。
此外,從箭葉鳳尾蕨乙酸乙酯抽出物中分離得到四個新化合物,十三個已知化合物,其中兩個蕨酸衍生物,針對人類血癌細胞(HL 60)具有細胞毒殺活性。
另一方面,藉由細胞毒殺活性導引分離,將雙花耳草分離得到三個新化合物,十九個已知化合物,將雙花耳草植物中分離得到的化合物進行細胞毒殺實驗,測試不同細胞,其中三個新香豆素化合物、ursolic acid、oleanolic acid 和6''-(??-sitosteryl-3-O-??-D-glucopyranosidyl)-pentadecanoate 針對人類肝癌細胞(Hep G2)及乳癌細胞(MDA-MB-231、MCF-7)具有抑制作用。
最後,本研究首次證明闊葉骨碎補和箭葉鳳尾蕨的DPPH捕捉活性,是來自其富含的多酚類化合物,並從箭葉鳳尾蕨和雙花耳草中發現新的具癌細胞生長抑制作用的化合物,所有分離得到的化合物皆藉由一維、二維核磁共振光譜等物理數據和部分化方法確認其化學結構。
Using the DPPH radical scavenging activity-guided fractionation method, four new compounds were isolated from the aqueous extract of Davallia solida rhizome (DS), together with ten known compounds. Among them, mangiferin derivatives have the strongest antioxidant activity. Solvent partition yielded of aqueous extract different layers and total phenol content. n-Butanol layer has the highest phenol content. Among the fourteen compouds of n-butanol, eleven compounds have stronger free radicals scavenging activity than vitamin E. The antioxidant activity of DS may be therefore attributed in part to its constituents.
Three new compounds were isolated from the aqueous extract of Pteris ensiformis Sword Brake fern (SBF) with five known compounds. HPLC with UV detector was further employed to analyze the content of each compound based on the retention time by comparison with isolated pure compounds. It was found that the most abundant phenolic compound in SBF were caffeic acid and hispidin derivatives. This data indicates that SBF is rich in phenolic antioxidants, especially derivatives of caffeic acid, hispidin and kaempferol.
The antioxidants from DS and SBF, together with their antioxidant activity were discussed for the SAR (Structure Activity Relationship).
Therefore, four new compounds, together with thirteen known compounds, were isolated from the ethyl acetate extract of Pteris ensiformis. Two pterosin derivatives.show cytotoxicity against HL 60 cells (human leukemia).
In the other hand, using the bioactivity-guided fractionation method, three new coumarin derivatives were isolated from the methanol extract of Hedyotis biflora (HB), together with nineteen known compounds. Three new compounds, ursolic acid, oleanolic acid, and 6''-(??-sitosteryl-3-O-??-D-glucopyranosidyl)- pentadecanoate showed cytotoxicity against human tumor cell lines, Hep G2, MCF-7, and MDA-MB-231.
In conclusion, we demonstrated for the first time that DS and SBF exhibited strong DPPH scavenging activity and fund out the new cytotoxic compounds from SBF and HB. The structural elucidation of all isolated compounds are base on spectroscopic analyses (mianly 1D and 2D NMR techniques), as well as chemical ecidence.
目錄-----------------------------------------------------Ⅰ
圖表目錄-------------------------------------------------Ⅲ
縮寫表---------------------------------------------------Ⅹ
中文摘要--------------------------------------------------I
英文摘要-------------------------------------------------II
第一章 緒言-----------------------------------------------1
第一節 闊葉骨碎補植物型態---------------------------------1
第二節 箭葉鳳尾蕨植物型態---------------------------------4
第三節 雙花耳草植物型態闊---------------------------------9
第二章 研究動機與目的------------------------------------11
第一節 闊葉骨碎補部分------------------------------------17
第二節 箭葉鳳尾蕨部分------------------------------------19
第三節 雙花耳草部分--------------------------------------20
第三章 藥理與化學成份研究回顧----------------------------21
第一節 闊葉骨碎補研究回顧--------------------------------21
第二節 箭葉鳳尾蕨研究回顧--------------------------------28
第三節 雙花耳草研究回顧---------------------------------41
第四章 萃取與分離---------------------------------- -----58
第一節 闊葉骨碎補成分抽取與分離--------------------------58
第二節 箭葉鳳尾蕨成分抽取與分離--------------------------61
第三節 雙花耳草成分抽取與分離----------------------------66
第五章 化合物之結構證明----------------------------------70
第一節 3''-O-p-Hydroxybenzoylmangiferin(1)之結構證明--70
第二節 4''-O-p-Hydroxybenzoylmangiferin(2)之結構證明--78
第三節 6''-O-p-Hydroxybenzoylmangiferin(3)之結構證明--86
第四節 3-O-p-Hydroxybenzoylmangiferin(4)之結構證明---94
第五節 Kaempferol 3-O-??-L-rhamnopyranoside-7-O-
[??-D-apiofuranosyl-(1-2)-??-D-glucopyranoside](15)
之結構證明----------------------------------------------102
第六節 7-O-Caffeoylhydroxymaltol 3-O-??-D-glucopyranoside (17)
之結構證明----------------------------------------------112
第七節 7-O-Cis-coumaroylhydroxymaltol 3-O-??-D-
glucopyranoside (18)之結構證明--------------------------120
第八節 Hispidin 4-O-D-glucopyranoside (21) 之結構證明-128
第九節?nD-Xylopyranosyl(1→2)-7-O-benzoyl-D-glucopyranoside (13) 之結構證明-------------------------135
第十節 4-O-Benzoyl-D-xylopyranosyl(1→2)-
7-O-benzoylD-glucopyranoside之結構證明------------------144
第十一節 2R,3R-Pterosin L 3-O-??-D-glucopyranoside (27)
第十二節 Hedyotiscone A(42)之結構證明-----------------153
第十三節 Hedyotiscone B(43)之結構證明-----------------161
第十四節 Hedyotiscone C(44)之結構證明-----------------170
第六章 生物活性相關試驗---------------------------------175
第一節 闊葉骨碎補塊莖與箭葉鳳尾蕨之抗氧化活性成分-------175
第二節 闊葉骨碎補塊莖與箭葉鳳尾蕨之抗氧化活性成分含量分析----------------------------------------------------------180
第三節 箭葉鳳尾蕨與雙花耳草之細胞毒殺試驗---------------183
第七章 結論---------------------------------------------186
第八章 相關實驗部分-------------------------------------188
儀器與材料---------------------------------------------188
第一節 闊葉骨碎補植物材料與分離純化---------------------190
第二節 箭葉鳳尾蕨植物材料與分離純化---------------------192
第三節 雙花耳草植物材料與分離純-------------------------194
第四節 抗氧化活性篩選試驗-------------------------------196
第五節 抗氧化活性成份含量試驗---------------------------198
第六節 細胞毒殺試驗之實驗方法---------------------------200第七節 各化合物之物理與光譜數據總-----------------------202
第九章 參考文獻-----------------------------------------246
論文發表-----------------------------------------------260
1.Li, H. L.; Liu, T. S.; Huang, T. C.; Koyama, T.; Devol, C. E., Flora of Taiwan (2nd ed.). Editorial Committee of Flora of Taiwan: Taipei, 1993; p186-198.
2.凌春暉, 骨碎補有效成分之研究. 國防大學碩士論文: 2003.
3.苗藥捲,邱德文,中華本草. 上海科學技術出版社: 2000; p 118-119.
4.郭城孟, 台灣維管束植物簡誌(一). 行政院農業委員會: 台北, 1997; p 36-51.
5.Li, H. L., Liu, T. S.; Huang, T. C.; Koyama, T.; Devol, C. E., Flora of Taiwan (2nd ed.). Editorial Committee of Flora of Taiwan: Taipei, 1993; p 206-233.
6.魏秀安, 箭葉鳳尾蕨及其有效成分抑制動脈粥樣硬化機轉之探討. 嘉南藥理科技大學碩士論文: 2006.
7.苗藥捲,邱德文,中華本草. 上海科學技術出版社: 2000; p 230-245.
8.Li, H. L., Liu, T. S.; Huang, T. C.; Koyama, T.; Devol, C. E., Flora of Taiwan (2nd ed.). Editorial Committee of Flora of Taiwan: Taipei, 1993; p 265-340.
9.楊政哲; 林俊清, Hedyotis diffusa 及其相關植物之抗氧化與活性研究. 高雄醫學大學博士論文1996.
10.邱年永, 百草茶原植物. 台中弘祥出版社, 1991; pp 141-144.
11.Chang, H.-C.; Huang, G.-J.; Agrawal, D. C.; Kuo, C.-L.; Wu, C.-R.; Tsay, H.-S., Antioxidant activities and polyphenol contents of six folk medicinal ferns used as "Gusuibu". Botanical Studies 2007, 48, (4), 397-406.
12.Tanaka, Y.; Kitajima, J.-i.; Ageta, H., Pharmacognostical studies on "Ku-tui-po". III. Constituents of the rhizomes of Davallia solida. Natural Medicines (Tokyo) 1998, 52, (5), 409-413.
13.Wang, G. Drynaria extract for treating osteoarthritis. 1005-7489 1733022, 20040813., 2006.
14.Renimel, I.; Olivier, M.; Andre, P. Use of davallia plant extract in cosmetic and pharmaceutical compositions for the treatment of skin aging. 96-15797 2757395, 19961220., 1998.
15.Boydron-Le Garrec, R.; Benoit, E.; Sauviat, M.-P.; Lewis, R. J.; Molgo, J.; Laurent, D., Ability of some plant extracts, traditionally used to treat ciguatera fish poisoning, to prevent the in vitro neurotoxicity produced by sodium channel activators. Toxicon 2005, 46, (6), 625-634.
16.Rancon, S.; Chaboud, A.; Darbour, N.; Comte, G.; Barron, D.; Raynaud, J.; Cabalion, P., A new C-glycosyl xanthone isolated from Davallia solida. Phytochemistry 1999, 52, (8), 1677-1679.
17.Hwang, T. H.; Kashiwada, Y.; Nonaka, G.; Nishioka, I., Tannins and related compounds. Part 89. 4-Carboxymethyl flavan-3-ols and procyanidins from Davallia divaricata. Phytochemistry 1990, 29, (1), 279-282.
18.Cui, C. B.; Tezuka, Y.; Kikuchi, T.; Nakano, H.; Tamaoki, T.; Park, J. H., Davallin, A new tetrameric proanthocyanidin from the rhizomes of Davallia mariesii Moore. Chemical & Pharmaceutical Bulletin 1991, 39, (8), 2179-2181.
19.Cui, C. B.; Tezuka, Y.; Kikuchi, T.; Nakano, H.; Tamaoki, T.; Park, J. H., Constituents of a fern, Davallia mariessi Moore. IV. Isolation and structures of a novel norcarotane sesquiterpene glycoside, a chromone glucuronide, and two epicatechin glycosides. Chemical & Pharmaceutical Bulletin 1992, 40, (8), 2035-2040.
20.Cui, C. V.; Tezuka, Y.; Kikuchi, T.; Nakano, H.; Park, J. H., Davallioside A and B, novel flavan-3-ol derivatives with a gamma -lactam, from the rhizomes of Davallia mariesii Moore. Chemical & Pharmaceutical Bulletin 1990, 38, (9), 2620-2622.
21.Cui, C. B.; Tezuka, Y.; Yamashita, H.; Kikuchi, T.; Nakano, H.; Tamaoki, T.; Park, J. H., Constituents of a fern, Davallia mariesii Moore. V. Isolation and structures of davallin, a new tetrameric proanthocyanidin, and two new phenolic glycosides. Chemical & Pharmaceutical Bulletin 1993, 41, (9), 1491-1497.
22.Shiojima, K.; Ageta, H., Fern constituents: two new triterpenoid hydrocarbons, hop-16-ene and isohop-22(29)-ene, isolated from Davallia mariesii. Chemical & Pharmaceutical Bulletin 1990, 38, (2), 347-349.
23.Lin, Y.-Y.; Kakisawa, H.; Shiobara, Y.; Nakanishi, K., Structure of davallic acid. Chemical & Pharmaceutical Bulletin 1965, 13, (8), 986-995.
24.Tanaka, Y.; Tohara, K.; Terasawa, K.; Sawada, M.; Ageta, H., Pharmacognostical studies on Ku-tsui-po. II. Shoyakugaku Zasshi 1978, 32, (4), 260-266.
25.Cui, C. B.; Tezuka, Y.; Kikuchi, T.; Nakano, H.; Tamaoki, T.; Park, J. H., Constituents of a fern, Davallia mariesii Moore. II. Identification and proton and carbon-13 nuclear magnetic resonance spectra of procyanidin B-5, epicatechin-(4beta ->8)-epicatechin-(4beta ->6)-epicatechin, and epicatechin-(4beta ->6)-epicatechin-(4beta ->8)-epicatechin-(4beta ->6)-epicatechin. Chemical & Pharmaceutical Bulletin 1992, 40, (4), 889-898.
26.Chen, G. G.; Liang, N. C.; Lee, J. F. Y.; Chan, U. P. F.; Wang, S. H.; Leung, B. C. S.; Leung, K. L., Over-expression of Bcl-2 against Pteris semipinnata L-induced apoptosis of human colon cancer cells via a NF-kappa B-related pathway. Apoptosis 2004, 9, (5), 619-627.
27.He, C.; Liang, N.; Mo, L.; Li, J.; Zhang, X., Apoptosis in HL-60 cells induced by compound 6F isolated from Pteris semipinnata L. Zhongliu Fangzhi Zazhi 2002, 9, (1), 11-14.
28.He, T.; Chen, J.; Mo, L.; Liang, N., Effects of a diterpenoid 5F from Pteris semipinnata on protein expression of nuclear factor-kB (NF-kB, P65) and Smad3 and mRNA expression of NF-kB (P65) and ETS-1 in highly metastatic ovarian carcinoma HO-8910PM cells. Zhongcaoyao 2005, 36, (4), 545-548.
29.He, C.; Liang, N.; Mo, L. e.; Li, J.; Zhang, X., Role of calcium ion in the cytotoxicity and DNA fragment induction in HL-60 cells by 6F isolated from Pteris semipinnata L. Zhongguo Bingli Shengli Zazhi 2002, 18, (9), 1025-1028.
30.Dalli, A. K.; Saha, G.; Chakraborty, U., Characterization of antimicrobial compounds from a common fern, Pteris biaurita. Indian Journal of Experimental Biology 2007, 45, (3), 285-290.
31.Kobayashi, A.; Egawa, H.; Koshimizu, K.; Mitsui, T., Antimicrobial constituents in Pteris inaequalis. Agricultural and Biological Chemistry 1975, 39, (9), 1851-6.
32.Lu, Y. N.; Jiang, G. X.; Wu, K. F; Deng, Y. F; Liang, N. C., Separation and identification of flavonoids in Pteris semipinnata L and their antioxidation activity. Huaxue Shijie 2007, 48, (4), 205-208, 220.
33.Srivastava, M.; Ma, L. Q.; Singh, N.; Singh, S., Antioxidant responses of hyper-accumulator and sensitive fern species to arsenic. Journal of Experimental Botany 2005, 56, (415), 1335-1342.
34.Wu, M.-J.; Weng, C.-Y.; Wang, L.; Lian, T.-W., Immunomodulatory mechanism of the aqueous extract of sword brake fern (Pteris ensiformis Burm.). Journal of ethnopharmacology 2005, 98, (1-2), 73-81.
35.Wei, H.-A.; Lian, T.-W.; Tu, Y.-C.; Hong, J.-T.; Kou, M.-C.; Wu, M.-J., Inhibition of Low-Density Lipoprotein Oxidation and Oxidative Burst in Polymorphonuclear Neutrophils by Caffeic Acid and Hispidin Derivatives Isolated from Sword Brake Fern. Journal of Agricultural and Food Chemistry 2007, 55, (26), 10579-10584.
36.Tanaka, N.; Murakami, T.; Saiki, Y.; Chen, C. M.; Gomez P, L. D., Chemical and chemotaxonomical studies of ferns. XXXVII. Chemical studies on the constituents of Costa Rican ferns. 2. Chemical & Pharmaceutical Bulletin 1981, 29, (12), 3455-63.
37.Imperato, F., A new flavone glycoside from the fern Pteris cretica. Experientia 1994, 50, (11/12), 1115-16.
38.Murakami, T.; Tanaka, N.; Chen, C.-M., Chemical and chemicotaxonomic studies of the genus Pteris and related genera (Pteridaceae). IX. Constituents of Pteris oshimensis. Chemical & Pharmaceutical Bulletin 1975, 23, (8), 1890-2.
39.Wij, M.; Rangaswami, S., Indanone derivatives from Pteris wallichiana. Indian Journal of Chemistry, Section B: Organic Chemistry Including Medicinal Chemistry 1977, 15, (1), 16-17.
40.Murakami, T.; Taguchi, S.; Nomura, Y.; Tanaka, N.; Satake, T.; Saiki, Y.; Chen, C.-M., Chemical and chemotaxonomic studies on the genus Pteris and related genera (Pteridaceae). XII. Additional Indan-1-one derivatives of the genus Pteris. Chemical & Pharmaceutical Bulletin 1976, 24, (8), 1961-4.
41.Aoyama, K.; Tanaka, N.; Suzuki, N.; Murakami, T.; Saiki, Y.; Chen, C.-M., Chemical and chemotaxonomic studies on the genus Pteris and related genera (Pteridaceae). XVI. New pterosin derivatives from P. wallichiana Agardh. and P. semipinnata L. Chemical & Pharmaceutical Bulletin 1977, 25, (9), 2461-4.
42.Murakami, T.; Aoyama, K.; Tanaka, N.; Chen, C.-M., Chemical and chemotaxonomic studies on the genus Pteris and related genera (Pteridaceae). X. Chemical studies on the constituents of Pteris wallichiana Agardh. Chemical & Pharmaceutical Bulletin 1976, 24, (1), 173-5.
43.Murakami, T.; Taguchi, S.; Chen, C.-M., Chemical and chemotaxonomic studies of the genus Pteris and related genera (Pteridaceae). XIV. Chemical studies on the constituents of Hypolepis punctata (Thunb.) Mett. Chemical & Pharmaceutical Bulletin 1976, 24, (9), 2241-2243.
44.Murakami, T.; Satake, T.; Ninomiya, K.; Iida, H.; Yamauchi, K.; Tanaka, N.; Saiki, Y.; Chen, C.-M., Chemical and chemotaxonomic studies of ferns. Part 25. Pterosin derivatives from the family Pteridaceae. Phytochemistry 1980, 19, (8), 1743-1746.
45.Satake, T.; Murakami, T.; Saiki, Y.; Chen, C.-M., Chemical and chemotaxonomic studies of the genus Pteris and related genera (Pteridaceae). Part XIX. Chemical studies of the constituents of Pteris vittata L. Chemical & Pharmaceutical Bulletin 1978, 26, (5), 1619-1622.
46.Saito, K.; Nagao, T.; Takatsuki, S.; Koyama, K.; Natori, S., The sesquiterpenoid carcinogen of bracken fern, and some analogs, from the Pteridaceae. Phytochemistry 1990, 29, (5), 1475-9.
47.Sengupta, P.; Sen, M.; Basak, M.; Pakrashi, S. C.; Ali, E., Chemical constituents of Pteris wallichiana. Indian Journal of Chemistry, Section B: Organic Chemistry Including Medicinal Chemistry 1976, 14B, (10), 817-818.
48.Murakami, T.; Tanaka, N.; Tezuka, T.; Chen, C.-M., Chemical and chemotaxonomic investigations of the genus Pteris and related genera. VIII. Chemical investigation of the constituents of Pteris inaequalis var aequata. Chemical & Pharmaceutical Bulletin 1975, 23, (7), 1634-1637.
49.Qin, B.; Zhu, D.-Y.; Jiang, S.-H.; Xiang, G.; Leng, Y.; Gu, Z.-P.; Wang, Y.-Q.; Shao, X.-F., Chemical constituents of Pteris multifida and their inhibitory effects on growth of rat prostatic epithelial cells in vitro. Zhongguo Tianran Yaowu 2006, 4, (6), 428-431.
50.Murakami, T.; Tanaka, N.; Tanaka, K.; Chen, C.-M., Pterosin Q and pteroside Q from Pteris oshimensis and Histiopteris incisa. Chemical & Pharmaceutical Bulletin 1974, 22, (11), 2758-2761.
51.Murakami, T.; Satake, T.; Chen, C.-M., Chemical and chemotaxonomic investigation of the genus Pteris and of the related genera. VI. Chemical investigation of the constituents of Pteris kiuschiuensis. Chemical & Pharmaceutical Bulletin 1975, 23, (4), 936-939.
52.Tanaka, N.; Kudo, M.; Taniguchi, T.; Murakami, T.; Saiki, Y.; Chen, C.-M., Chemical and chemotaxonomical studies of the family Pteris and related families (Pteridaceae). XVIII. Chemical study of the constituents of Pteris ryukyuensis Tagawa and Pteris longipinna Hayata. Chemical & Pharmaceutical Bulletin 1978, 26, (4), 1339-1342.
53.Tanaka, N.; Satake, T.; Takahashi, A.; Mochizuki, M.; Murakami, T.; Saiki, Y.; Yang, J. Z.; Chen, C. M., Chemical and chemotaxonomical studies of ferns. XXXIX. Chemical studies on the constituents of Pteris bella Tagawa and Pteridium aquilinum subsp. wightianum (Wall) Shich. Chemical & Pharmaceutical Bulletin 1982, 30, (10), 3640-3646.
54.Murakami, T.; Tanaka, N.; Komazawa, Y.; Saiki, Y.; Chen, C. M., Chemical and chemotaxonomic studies of fronds. XLI. Additional compounds from Pteris purpureorachis Copel. Chemical & Pharmaceutical Bulletin 1983, 31, (5), 1502-1504.
55.Wang, J.; Liang, N.; Mo, L. e., Effects of a diterpenoid compound 5F isolated from Pteris semipinnata L. on the expressions of several oncogenes of K562 cells. Zhongguo Yaolixue Tongbao 2002, 18, (4), 418-421.
56.Sen, M.; Sarkar, U., Chemical constituents of Pteris longifolia. Journal of the Indian Chemical Society 1978, 55, (3), 308-309.
57.Murakami, T.; Kudo, M.; Taguchi, S.; Tanaka, N.; Saiki, Y.; Chen, C.-M., Chemical and chemotaxonomic studies on the genus Pteris and related genera (Pteridaceae). XVII. Additional constituents of Pteris inaequalis var. aequata (Miq.) Tagawa. Chemical & Pharmaceutical Bulletin 1978, 26, (2), 643-645.
58.He, T.; Mo, L.; Liang, N., Effects of 5F from Pteris semipinnata L on expression of NF-kB and FAK proteins in HO-8910PM cells. Zhongliu Fangzhi Zazhi 2005, 12, (8), 565-568.
59.Gemmrich, A. R., Fatty acid composition of fern spore lipids. Phytochemistry 1977, 16, (7), 1044-1046.
60.Zhang, W.; Cai, Y., Purification and Characterization of Thiols in an Arsenic Hyperaccumulator under Arsenic Exposure. Analytical Chemistry 2003, 75, (24), 7030-7035.
61.Hu, H.; Jian, Y.; Zheng, X.; Liu, J.; Cao, H., A new sesquiterpene glycoside from the roots of Pteris multifida poir. Indian J. Chem., Sect. B: Org. Chem. Incl. Med. Chem. 2006, 45B, (5), 1274-1277.
62.Kim, Y.; Park, E. J.; Kim, J.; Kim, Y.-B.; Kim, S. R.; Kim, Y. C., Neuroprotective Constituents from Hedyotis diffusa. Journal of Natural Products 2001, 64, (1), 75-78.
63.Permana, D.; Lajis, N. H.; Abas, F.; Othman, A. G.; Ahmad, R.; Kitajima, M.; Takayama, H.; Aimi, N., Antioxidative constituents of Hedyotis diffusa Willd. Natural Product Sciences 2003, 9, (1), 7-9.
64.Saha, K.; Lajis, N. H.; Israf, D. A.; Hamzah, A. S.; Khozirah, S.; Khamis, S.; Syahida, A., Evaluation of antioxidant and nitric oxide inhibitory activities of selected Malasyian medicinal plants. J Ethnopharmacol 2004, 92, (2-3), 263-267.
65.Lu, C. M.; Yang, J. J.; Wang, P. Y.; Lin, C. C., A new acylated flavonol glycoside and antioxidant effects of Hedyotis diffusa. Planta Medica 2000, 66, (4), 374-7.
66.Hamzah, A. S.; Lajis, N. H.; Sargent, M. V., Kaempferitrin from the leaves of Hedyotis verticillata and its biological activity. Planta Medica 1994, 60, (4), 388-9.
67.Hamzah, A. S.; Aimi, N.; Lajis, N. H. J., Constituents of Hedyotis herbacea (Rubiaceae). Biochemical Systematics and Ecology 1996, 24, (3), 273-279.
68.Kikuchi, T.; Matsuda, S.; Kadota, S.; Sakai, Y.; Namba, T.; Watanabe, K.; Dissanayake, D. M. R. B., Studies on the constituents of medicinal and related plants in Sri Lanka. I. New triterpenes from Hedyotis lawsoniae. Chemical & Pharmaceutical Bulletin 1984, 32, (10), 3906-3911.
69.Hui, W.-H.; Li, M.-M., An examination of the Rubiaceae of Hong Kong. Triterpenoids and sterols from the stems of Hedyotis acutangula. Phytochemistry 1977, 16, (8), 1309-1310.
70.Fang, Z.; Yang, Y.; Zhou, G., Isolation and identification of chemical constituents from Hedyotis chrysotricha (Palib.) Merr. Zhongguo zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 1992, 17, (2), 98-100.
71.Fang, Z.; Yang, Y.; Zhou, G., Isolation and identification of chemical constituents from Hedyotis chrysotricha (III) Merr.. Zhongguo Zhong Yao Za Zhi 1993, 18, (2), 120-127.
72.Kal''yan, B. N.; Lazur''evskii, G. V., Ursolic and 3-epiursolic acids from waste material of lavender essential oil production. Khim Prir Soedin. 1968, 4, (1), 53-54.
73.Jabbar, A.; Akhteruzzaman, S. M.; Rashid, M. A., Constituents of Hedyotis scandens. Fitoterapia 1996, 67, (3), 278-279.
74.Fang, Z.; Yang, Y.; Zhou, G., Isolation and identification of chemical constituents from Hedyotis chrysotricha (II) Merr. Zhongguo Zhongyao Zazhi 1995, 12, (3), 95-100.
75.Konishi, M.; Hano, Y.; Takayama, M.; Nomura, T.; Hamzah, A. S.; Ahmad, R. B.; Jasmani, H., Triterpenoid saponins from Hedyotis nudicaulis. Phytochemistry 1998, 48, (3), 525-528.
76.Lu, C.-M.; Yang, J.-J.; Wang, P.-Y.; Lin, C.-C., A new acylated flavonol glycoside and antioxidant effects of Hedyotis diffusa. Planta Medica 2000, 66, (4), 374-377.
77.Dominguez, X. A.; Sanchez V, H.; Palacios Estrada, T., Chemical studies on Hedyotis intricata. Rubiaceae. Revista Latinoamericana de Quimica 1992, 23, (1), 46-47.
78.Peng, J. N.; Feng, X. Z.; Liang, X. T., A new iridoid from Hedyotis crysotrichae. Chinese Chemical Letters 1995, 6, (11), 965-966.
79.Takagi, S.; Yamaki, M.; Nishihama, Y.; Ishiguro, K., Iridoid glucosides of the Chinese drug Bai Hua She She Cao (Hedyotis diffusa Willd.). Shoyakugaku Zasshi 1982, 36, (4), 366-369.
80.Peng, J.-N.; Feng, X.-Z.; Liang, X.-T., Two New Iridoids from Hedyotis chrysotricha. Journal of Natural Products 1999, 62, (4), 611-612.
81.Takagi, S.; Yamaki, M.; Masuda, K.; Nishihama, Y.; Sakina, K., Studies on the herb medical materials used for some tumors. II. On the constituents of Hedyotis corymbosa Lam. Yakugaku Zasshi 1981, 101, (7), 657-659.
82.Peng, J.-N.; Feng, X.-Z.; Liang, X.-T., Iridoids from Hedyotis hedyotidea. Phytochemistry 1998, 47, (8), 1657-1659.
83.Peng, J. N.; Feng, X. Z.; Liang, X. T., Two new iridoids from Hedyotis chrysotricha. J Nat Prod 1999, 62, (4), 611-612.
84.Peng, J.; Feng, X.; Li, G.; Liang, X., Chemical investigation of genus Hedyotis II.isolation and identification of iridoids from Hedyotis chrysotricha. Yaoxue Xuebao 1997, 32, (12), 908-913.
85.Nishihama, Y.; Masuda, K.; Yamaki, M.; Takagi, S.; Sakina, K., Three new iridoid glucosides from Hedyotis diffusa. Planta Medica 1981, 43, (1), 28-33.
86.Zhao, J.-F.; Yuan, Q.-M.; Yang, X.-D.; Zhang, H.-B.; Li, L., Two new iridoid glycosides from Hedyotis tenelliflora Blume. Helvetica Chimica Acta 2005, 88, (9), 2532-2536.
87.Jiang, W.; Kuang, L.-S.; Hou, A.-J.; Qian, M.; Li, J.-Z., Iridoid glycosides from Hedyotis corymbosa. Helvetica Chimica Acta 2007, 90, (7), 1296-1301.
88.Ahmad, R.; Shaari, K.; Lajis, N. H.; Hamzah, A. S.; Ismail, N. H.; Kitajima, M., Anthraquinones from Hedyotis capitellata. Phytochemistry 2005, 66, (10), 1141-1147.
89.Huang, W.; Li, Y.; Jiang, J., Chemical constituents from Hedyotis diffusa. Zhongguo Zhongyao Zazhi 2008, 33, (5), 524-526.
90.Ratnagiriswaran, A. N.; Venkatachalam, K., Auricularine - a new alkaloid from the roots and stems of Hedyotis auricularia. Journal Indian Chemistry 1942, 19, 389-392.
91.Purushothaman, K. K.; Sarada, A., Structure of auricularine, a bis-indole alkaloid from Hedyotis auricularia. Phytochemistry 1981, 20, (2), 351-352.
92.Phuong, N. M.; Van Sung, T.; Porzel, A.; Schmidt, J.; Merzweiler, K.; Adam, G., b-Carboline alkaloids from Hedyotis capitellata. Phytochemistry 1999, 52, (8), 1725-1729.
93.Nguyen Minh, P.; Tran Van, S.; Schmidt, J.; Porzel, A.; Adam, G., Capitelline. A new indole alkaloid from Hedyotis capitellata. Natural Product Letters 1998, 11, (2), 93-100.
94.Nguyen Minh, P.; Tran Van, S.; Porzel, A.; Schmidt, J.; Adam, G., Two new b-carboline alkaloids from Hedyotis capitellata var. mollis. Planta Medica 1999, 65, (8), 761-762.
95.Hui, W. H.; Lam, C. N., The Rubiaceae of Hongkong. II. Triterpenoids and steroids from Hedyotis acutangula. Phytochemistry 1965, 4, (2), 333-335.
96.Catalano, S.; Luschi, S.; Flamini, G.; Cioni, P. L.; Nieri, E. M.; Morelli, I., A xanthone from Senecio mikanioides leaves. Phytochemistry 1996, 42, (6), 1605-1607.
97.Rancon, S.; Chaboud, A.; Darbour, N.; Comte, G.; Barron, D.; Raynaud, J.; Cabalion, P., A new C-glycosyl xanthone isolated from Davallia solida. Phytochemistry 1999, 52, (8), 1677-1679.
98.Hwang, T. H.; Kashiwada, Y.; Nonaka, G.; Nishioka, I., Tannins and related compounds. Part 89. 4-Carboxymethyl flavan-3-ols and procyanidins from Davallia divaricata. Phytochemistry 1990, 29, (1), 279-282.
99. Hsu, F. L.; Chen, J. Y., Phenolics from Tectaria subtriphylla. Phytochemistry 1993, 34, (6), 1625-1627.
100. Iorizzi, M.; Lanzotti, V.; De Marino, S.; Zollo, F.; Blanco-Molina, M.; Macho, A.; Munoz, E., New glycosides from Capsicum annuum L. var. acuminatum. Isolation, structure determination, and biological activity. Journal of Agricultural and Food Chemistry 2001, 49, (4), 2022-2029.
101. Miyase, T.; Ueno, A.; Takizawa, N.; Kobayashi, H.; Oguchi, H., Studies on the glycosides of Epimedium grandiflorum Morr. var. thunbergianum (Miq.) Nakai. III. Chemical & Pharmaceutical Bulletin 1988, 36, (7), 2475-2484.
102. Kolonko, K. J.; Deinzer, M. L.; Miller, T. L., Preparation of chlorinated phenoxyphenols. Synthesis 1981, (2), 133-135.
103. Lamb, P. N-Acylazetidine derivatives as MEK inhibitors and their preparation, pharmaceutical compositions and use in the treatment of cancer. 2007-US25751 2008076415, 20071214., 2008.
104. El-Sayed, N. H.; Awaad, A. S.; Hifnawy, M. S.; Mabry, T. J., A flavonol triglycoside from Chenopodium murale. Phytochemistry 1999, 51, (4), 591-593.
105.Kumaran, A.; Karunakaran, R. J., Activity-guided isolation and identification of free radical-scavenging components from an aqueous extract of Coleus aromaticus. Food Chemistry. 2006, 100, (1), 356-361.
106.Iwai, K.; Kishimoto, N.; Kakino, Y.; Mochida, K.; Fujita, T., In vitro antioxidative effects and tyrosinase inhibitory activities of seven hydroxycinnamoyl derivatives in green coffee beans. Journal of Agricultural and Food Chemistry 2004, 52, (15), 4893-4898.
107.Guo, H.; Koike, K.; Li, W.; Guo, D.; Nikaido, T., Maltol glucosides from the tuber of Smilax bockii. Phytochemistry (Elsevier) 2004, 65, (4), 481-484. 108 Norbæk, R.; Nielsen, K.; Kondo, T. Anthocyanins from flowers of Cichorium intybus. Phytochemistry 2002, 60, (4), 357-359.
108.Norbaek, R.; Nielsen, K.; Kondo, T., Anthocyanins from flowers of Cichorium intybus. Phytochemistry 2002, 60, (4), 357-359.
109.Cantillo-Ciau, Z.; Brito-Loeza, W.; Quijano, L., Triterpenoids from Tillandsia fasciculata. Journal of Natural Products 2001, 64, (7), 953-955.
110.Nahrstedt, A.; Rockenbach, J.; Wray, V., Phenylpropanoid glycosides, a furanone glucoside and geniposidic acid from members of the Rubiaceae. Phytochemistry 1995, 39, (2), 375-378.
111.Tanaka, N.; Satake, T.; Takahashi, A.; Mochizuki, M.; Murakami, T.; Saiki, Y.; Yang, J. Z.; Chen, C. M., Chemical and chemotaxonomical studies of ferns. XXXIX. Chemical studies on the constituents of Pteris bella Tagawa and Pteridium aquilinum subsp. wightianum (Wall) Shich. Chemical & Pharmaceutical Bulletin 1982, 30, (10), 3640-3646.
112.Cantillo-Ciau, Z.; Brito-Loeza, W.; Quijano, L., Triterpenoids from Tillandsia fasciculata. Journal of Natural Products 2001, 64, (7), 953-955.
113.Chang, Y.-C.; Chang, F.-R.; Wu, Y.-C., The constituents of Lindera glauca. J. Chinese. Chemistry Sociation. 2000, 47, (2), 373-380.
114.Cabrita, L.; Andersen, O. M., Anthocyanins in blue berries of Vaccinium padifolium. Phytochemistry 1999, 52, (8), 1693-1696.
115.Sang, S.; Lapsley, K.; Rosen, R. T.; Ho, C.-T., New Prenylated Benzoic Acid and Other Constituents from Almond Hulls (Prunus amygdalus Batsch). Journal of Agricultural and Food Chemistry 2002, 50, (3), 607-609.
116.Siddiqui, B. S.; Sultana, I.; Begum, S., Triterpenoidal constituents from Eucalyptus camaldulensis var. obtusa leaves. Phytochemistry 2000, 54, (8), 861-865.
117.Afek, U.; Sztejnberg, A.; Carmely, S., 6,7-Dimethoxycoumarin, a citrus phytoalexin conferring resistance against Phytophthora gummosis. Phytochemistry 1986, 25, (8), 1855-1856..
118.Abe, F.; Chen, R.-F.; Yamauchi, T.; Ohashi, H., Iridoids from the roots of Thevetia peruviana. Chemical & Pharmaceutical Bulletin 1995, 43, (3), 499-500.
119.Wu, T.-S.; Lin, D.-M.; Shi, L.-S.; Damu Amooru, G.; Kuo, P.-C.; Kuo, Y.-H., Cytotoxic anthraquinones from the stems of Rubia wallichiana Decne. Chemical & Pharmaceutical Bulletin 2003, 51, (8), 948-950.
120.Hahn, R.; Nahrstedt, A., Hydroxycinnamic acid derivatives, caffeoylmalic and new caffeoylaldonic acid esters, from Chelidonium majus. Planta Medica. 1993, 59, (1), 71-75.
121.Dini, I.; Carlo Tenore, G.; Dini, A., Phenolic constituents of Kancolla seeds. Food Chemistry 2003, 84, (2), 163-168.
122.Kakuda, R.; Yaoita, Y.; Machida, K.; Kikuchi, M., Structural analysis on the constituents of Lonicera species. XVI. On the chemical constituents of the flower buds of Lonicera japonica thumb. (3). Journal Tohoku Pharmcy University. 2000, 47, (2),55-60.
123.Lin, F., A novel photoproduct of uracil in phosphate-buffered saline. Chinese Chemistry. Letter 2000, 11, (8), 679-680.
124.Hisamoto, M.; Kikuzaki, H.; Ohigashi, H.; Nakatani, N., Antioxidant compounds from the leaves of Peucedanum japonicum thunb. Journal of Agricultural and Food Chemistry 2003, 51, (18), 5255-5261.
125.Ulubelen, A.; Oksuz, S.; Mericli, A. H., Palmitic acid ester of sitosteryl 3.beta.-glucoside from Centaurea regia. Phytochemistry 1988, 27, (12), 3964-3965.
126.Rancon, S.; Chaboud, A.; Darbour, N.; Comte, G.; Bayet, C.; Simon, P. N.; Raynaud, J.; Di Pietro, A.; Cabalion, P.; Barron, D., Natural and synthetic benzophenones: interaction with the cytosolic binding domain of P-glycoprotein. Phytochemistry 2001, 57, (4), 553-557.
127.Ali, N. A. A.; Jansen, R.; Pilgrim, H.; Liberra, K.; Lindequist, U., Hispolon, a yellow pigment from Inonotus hispidus. Phytochemistry 1996, 41, (3), 927-929.
128.Roen, A.; Padron Juan, I.; Vazquez Jesus, T., Hydroxymethyl rotamer populations in disaccharides. Journal Organic Chemistry 2003, 68, (12), 4615-4630.
129.Yamaguchi, S.; Miyata, A.; Ueno, M.; Hase, T.; Yamamoto, K.; Kawase, Y., Acylations of 2-isopropenyl-2,3-dihydrobenzofurans. Chemical & Pharmaceutical Bulletin. 1984, 57, (2), 617-618.
130.Yamaguchi, S.; Muro, S.; Kobayashi, M.; Miyazawa, M.; Hirai, Y., Absolute Structures of Some Naturally Occurring Isopropenyldihydrobenzofurans, Remirol, Remiridiol, Angenomalin, and Isoangenomalin. Journal Organic Chemistry 2003, 68, (16), 6274-6278.
131.Castelluccio, C.; Paganga, G.; Melikian, N.; Bolwell, G. P.; Pridham, J.; Sampson, J.; Rice-Evans, C., Antioxidant potential of intermediates in phenylpropanoid metabolism in higher plants. FEBS Letter 1995, 368, (1), 188-192.
132.Hseu, Y.-C.; Chang, W.-C.; Hseu, Y.-T.; Lee, C.-Y.; Yech, Y.-J.; Chen, P.-C.; Chen, J.-Y.; Yang, H.-L., Protection of oxidative damage by aqueous extract from Antrodia camphorata mycelia in normal human erythrocytes. Life Science. 2002, 71, (4), 469-482.
133.Moller, J. K. S.; Madsen, H. L.; Aaltonen, T.; Skibsted, L. H., Dittany (Origanum dictamnus) as a source of water-extractable antioxidants. Food Chemistry 1998, 64, (2), 215-219.
134.Vinson, J. A.; Hao, Y.; Su, X.; Zubik, L., Phenol Antioxidant Quantity and Quality in Foods: Vegetables Journal of Agricultural and Food Chemistry. 1998, 46, (9), 3630-3634.
135.Kelner, M. J.; McMorris, T. C.; Taetle, R., Preclinical evaluation of illudins as anticancer agents: basis for selective cytotoxicity. Journal of the National Cancer Institute 1990, 82, (19), 1562-1565.
136.Kobayashi, A.; Egawa, H.; Koshimizu, K.; Mitsui, T., Antimicrobial constituents in Pteris inaequalis. Agricultural and Biological Chemistry 1975, 39, (9), 1851-1856.
137.Lee, T. T.; Kashiwada, Y.; Huang, L.; Snider, J.; Cosentino, M.; Lee, K. H., Suksdorfin: an anti-HIV principle from Lomatium suksdorfii, its structure-activity correlation with related coumarins, and synergistic effects with anti-AIDS nucleosides. Bioorganic & Medicinal Chemistry 1994, 2, (10), 1051-1056.
138.Tada, Y.; Shikishima, Y.; Takaishi, Y.; Shibata, H.; Higuti, T.; Honda, G.; Ito, M.; Takeda, Y.; Kodzhimatov, O. K.; Ashurmetov, O.; Ohmoto, Y., Coumarins and gamma-pyrone derivatives from Prangos pabularia: antibacterial activity and inhibition of cytokine release. Phytochemistry 2002, 59, (6), 649-654.
139.Ito, C.; Otsuka, T.; Ruangrungsi, N.; Furukawa, H., Chemical constituents of Micromelum minutum. Isolation and structural elucidation of new coumarins. Chemical & Pharmaceutical Bulletin 2000, 48, (3), 334-338.
140.Dinis, T. C. P.; Madeira, V. M. C.; Almeida, L. M., Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Archives of Biochemistry and Biophysics 1994, 315, (1), 161-169.
141.Parejo, I.; Viladomat, F.; Bastida, J.; Rosas-Romero, A.; Saavedra, G.; Murcia, M. A.; Jimenez, A. M.; Codina, C., Investigation of Bolivian plant extracts for their radical scavenging activity and antioxidant activity. Life Science 2003, 73, (13), 1667-1681.
142.Re, R.; Pellegrini, N.; Proteggente, A.; Pannala, A.; Yang, M.; Rice-Evans, C., Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine 1999, 26, (9/10), 1231-1237.
143.Waterman, P. G.; Mole, S.; Editors, Analysis of Phenolic Plant Metabolites. (Methods in Ecology). 1994; p 248 pp.
144.Sladowski, D.; Steer, S. J.; Clothier, R. H.; Balls, M., An improved MTT assay. Journal of Immunological Methods 1993, 157, (1-2), 203-207.
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top