跳到主要內容

臺灣博碩士論文加值系統

(3.236.124.56) 您好!臺灣時間:2021/07/30 06:37
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:史守正
研究生(外文):Shoh-Cheng Shih
論文名稱:血管內皮生長因子丙與促進性抗乙型淋巴毒素受體抗體對實驗性淋巴水腫小鼠尾巴的效果
論文名稱(外文):Effect of VEGF-C and agonistic anti-lymphotoxin beta receptor antibody on experimentally-induced lymphedema of mouse tails
指導教授:陳國熏陳國熏引用關係
指導教授(外文):Gwo-Shing Chen
學位類別:碩士
校院名稱:高雄醫學大學
系所名稱:醫學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:42
中文關鍵詞:淋巴水腫小鼠尾巴模式促進性抗乙型淋巴毒素受體抗體血管內皮生長因子丙oxazolone過敏性接觸性皮膚炎淋巴結新生淋巴管新生
外文關鍵詞:lymphedemamouse tail modelagonistic anti-lymphotoxin beta receptor antibodyVEGF-Coxazoloneallergic contact dermatitislymphoid organogenesislymphangiogenesis
相關次數:
  • 被引用被引用:0
  • 點閱點閱:185
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
  在治療人類乳癌與骨盆腔惡性腫瘤上,為避免腫瘤經由淋巴系統轉移,常會摘除鄰近腫瘤的引流淋巴結且在術後進行放射治療,因而造成四肢的醫源性淋巴水腫。長期的慢性淋巴水腫除了造成患肢不適外,也會有纖維化與局部免疫低下等後遺症。此種淋巴水腫目前主要以物理治療為主,雖然效果明確但療效有限且耗時。為治療此種淋巴水腫,以小鼠尾巴模式模擬人體四肢並以接近人類做法的手術方式阻斷淋巴引流以引起淋巴水腫,嘗試以促進性抗乙型淋巴毒素受體抗體(agonistic anti-lymphotoxin beta receptor antibody)誘發淋巴結新生及基因重組血管內皮生長因子丙(recombinant VEGF-C)誘發淋巴管新生來治療淋巴水腫,並以oxazolone外擦於尾巴皮膚以企圖引起過敏性接觸性皮膚炎來評估局部皮膚免疫力。結果雖然此種手術方式能使小鼠尾巴產生顯著的淋巴水腫,但促進性抗乙型淋巴毒素受體抗體與血管內皮生長因子丙的治療並未顯著改變小鼠尾巴體積的變化,且在組織學上也未發現有代表皮膚炎的表淺發炎性細胞浸潤與類似淋巴結的構造。顯示現有做法雖然能成功誘發淋巴水腫但無法有效加以改善且無法誘發淋巴結新生與過敏性接觸性皮膚炎。
In the treatment of breast cancer and pelvic malignancies in human, excision of draining lymph nodes and postsurgical radiotherapy are the procedures to prevent metastasis of cancer cells through the lymphatic system and cause iatrogenic lymphedema of limbs. Chronic lymphedema of longterm cause not only discomfort of affected limbs, but also sequelae like fibrosis and compromised local immunity. This kind of lymphedema was primarily handled by physiotherapy. Although physiotherapy is definitely effective but limited and time-consuming. In order to try to treat this kind of lymphedema, we used a mouse tail model to emulate human limbs and used a surgical method similar to procedures performed in human to block lymphatic drainage to induce lymphedema. We tried to use the treatment of an agonistic anti-lymphotoxin beta receptor antibody to induce lymphoid organogenesis and recombinant VEGF-C to induce lymphangiogenesis to treat this lymphedema, and followed by topical application of oxazolone on tail skin in attempt to induce allergic contact dermatitis to evaluate local skin immunity. The results showed that although significant lymphedema was induced by current surgical method, there were no significant effects on changes of mouse tail volumes by agonistic lymphotoxin beta receptor antibody and VEGF-C. The histopathology of the tail skins did not show superficial inflammatory infiltrate that represent dermatitis or lymphoid organ-like structure. It showed that although siginificant lymphedema can be induced by current methods, these treatments can not improve the lymphedema, and neither lymphoid organogenesis nor allergic contact dermatitis can be induced by current methods.
摘要--------------------------------------------------------------04
前言--------------------------------------------------------------08
材料與方法-----------------------------------------------------12
結果--------------------------------------------------------------19
討論--------------------------------------------------------------22
結論--------------------------------------------------------------26
參考文獻--------------------------------------------------------27
附錄--------------------------------------------------------------29
1.Alitalo K, Tammela T et al.: Lymphangiogenesis in development and human disease. Nature 2005;438:946-53.
2.Jeltsch M, Tammela T et al.: Genesis and pathogenesis of lymphatic vessels. Cell Tissue Res 2003;314:69-84.
3.Saharinen P, Tammela T et al.: Lymphatic vasculature: development, molecular regulation in tumor metastasis an inflammation. Trends Cell Biol 2005;15:434-41.
4.Aalami OO, Allen DB et al.: Chylous ascites: a collective review. Surgery 2000;128:761-78.
5.Tuomas T, Tatiana V et al.: Molecular lymphangiogenesis: new players. Trends Cell Biol 2005;15:434-41.
6.Satoshi H, Michael D: New insight into the biology and pathology of cutaneous lymphatic system. J Dermatol Sci 2004;35:1-8.
7.Saaristo A, Veikkola T et al: Lymphangiogenic gene therapy with minimal blood vascular side effect. J Exp Med 2006;16:713-8.
8.Szuba A, Skobe M et al.: Therapeutic lymphangiogenesis with human recombinant VEGF-C. FASEB J 2002;16:1985-7.
9.Sarristo A, Tammela T et al.: Vascular endothelial growth factor-C gene therapy restores lymphatic flow across incision wound. FASEB J 2004;18:1707-9.
10.Cheung L, Hang J et al.: An experimental model for the study of lymphedema and its response to therapeutic lymphangiogenesis. BioDrugs 2006;20:363-70
11.Sumner A, Annick DVA et al.: Return of lymphatic function after flap transfer for acute lymphedema. Ann Surg 1999;229:421-7
12.Fu K, Izquierdo R et al.: Transplantation of lymph node fragments in a rabbit ear lymphedema model: a new method for restoring the lymphatic pathway. Plast Reconsstr Surg 1998;101:134-41.
13.Hu XQ, Jiang ZH et al.: Experimental study of VEGF-C gene for the treatment of chronic obstructive lymphedema in mouse tail model. Chinese J Plastic Surgery 2008;24:207-11.
14.Tumanov AV, Kuprash DV et al.: The role of lymphotoxin in development and maintenance of secondary lymphoid tissue. Cytokine Growth Factor Rev 2003;14:275-88.
15.Cupedo T, Jansen W et al.: Induction of secondary and tertiary lymphoid structures in the skin. Immunity 2004;21:655-667.
16.Thaunat O, Kerjasschiki D et al.: Is defective lymphatic drainage a trigger for lymphoid neogenesis? Trends Immunol 2006;27:441-5.
17.Goldsmith HS, de los Santos R: Omental transposition for the treatment of chronic lymphedema. Rev Surg 1996;23:303-4.
18.Goldsmith HS: Long term evaluation of omental transposition for chronic lymphedema.
19.Nielubowicz J, Olszewksi W et al.: Surgical lympho-venous shunt shunts. J Cardiovasc Surg 1968;9:262-7.
20.Campisi C, Boccardo F et al.: Reconstructive microsurgery of lymph vessels: the personal method of lymphatic-venous-lymphatic interpositioned graft shunt. Microsurgery 1995;16:161-6.
21.Tabibiazar R, Cheun L et al.: Inflammatory manifestations of experimental lymphatic insufficiency. Plos Med 2007;3:1114-39.
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
無相關期刊