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研究生:王尚任
研究生(外文):Shang-Jen Wang
論文名稱:製備多層壁奈米碳管奈米複合材料作為尼古丁與尿素感測器之探討
論文名稱(外文):Preparation of multiwalled carbon nanotube nanocomposites for nicotine and urea sensors
指導教授:蔡毓楨
學位類別:碩士
校院名稱:國立中興大學
系所名稱:化學工程學系所
學門:工程學門
學類:化學工程學類
論文種類:學術論文
畢業學年度:97
語文別:中文
論文頁數:88
中文關鍵詞:多層壁奈米碳管尼古丁尿素感測器
外文關鍵詞:Multiwalled carbon nanotubeNicotineUreaSensor
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本論文第一部分中以多層壁奈米碳管(multiwalled carbon nanotubes, MWNTs)-三氧化二鋁包覆二氧化矽奈米顆粒(alumina-coated silica, ACS)複合材料修飾玻璃碳電極(glassy carbon electrode, GCE)電催化氧化尼古丁,經由場發式電子顯微鏡的微觀表面形貌分析,可以確定帶正電荷之ACS奈米顆粒確實可圍繞於MWNTs之上,且可有效的將MWNTs彼此間分散開來。由結果顯示出MWNTs可催化尼古丁物質之電化學反應,MWNTs-ACS奈米複合材料修飾電極對於尼古丁的催化表現是由循環伏安法及安培法所證明,經由選擇出最佳的pH 8.0值及最佳測量電位0.7 V作為測量尼古丁濃度,其MWNTs-ACS奈米複合材料修飾GCE可有效降低電化學氧化電位,並防止電極表面毒化以及提高電流訊號。以最適值所製備出的MWNTs-ACS奈米複合材料薄膜對於偵測nicotine具其靈敏度達1.786 A M-1cm-2,線性範圍達690 μM、偵測極限達1.42 μM。
本論文第二部份以利用最適當的MWNTs-幾丁聚醣(Chitosan)複合材料基質圈入尿素酶(Urease, Urs)修飾於GCE上作為電流式尿素生物感測器,經由原子力顯微鏡(atomic force microscope, AFM)觀察其表面形貌,進而了解Urs在MWNTs-Chitosan薄膜中扮演的角色,並得知Urs能吸附於奈米碳管之上,且Urs均勻的分散在薄膜表面。MWNTs-Chitosan-Urs奈米生物複合薄膜修飾電極偵測尿素(Urea)。此複合薄膜中奈米碳管展現出優勢的偵測表現是由循環伏安法及安培法所證明。為了獲得本感測器之最佳化條件,經由探討幾個實驗條件的最適值(如操作電壓、緩衝溶液之pH值及酵素承載量)。本MWNTs-Chitosan-Urs奈米生物複合薄膜修飾GCE之尿素生物感測器其線性範圍從30到610 μM,而靈敏度為20.4 A M-1cm-2,應答時間約25秒。
Electrocatalytic oxidation of nicotine at multiwalled carbon nanotube (MWCNT)-alumina-coated silica (ACS) nanocomposite modified glassy carbon electrode are described. The dispersed MWNTs was characterized by transmission electron microscopy. The sensing performance of the MWCNT-ACS nanocomposite modified glassy carbon electrode for the electrooxidation of nicotine was investigated using cyclic voltammetry and amperometry in 0.1 M phosphate buffer solution (pH 8). The MWCNT-ACS nanocomposite modified glassy carbon electrode exhibited the abilities to decrease the electrooxidation potential, to prevent the electrode surface fouling, and to raise the current responses. The MWCNT-ACS nanocomposite responded rapidly to nicotine with a sensitivity of 1.786 A M-1cm-2, a linear range up to 690 μM and a detection limit of 1.42 μM.
A composite of multiwalled carbon nanotubes-chitosan (MWNTs-Chitosan) was used as a matrix for entrapment of urease (Urs) onto a glassy carbon electrode in order to fabricate amperometric biosensor. The homogeneity of the resulting nanobiocomposite film was characterized by atomic force microscopy (AFM). The performance of the MWNTs-Chitosan-Urs nanobiocomposite modified glassy carbon electrode was examined using cyclic voltammetry and amperometry in presence of Urea. The influence of several experimental parameters such as enzyme loading, solution pH value , applied potential was explored to optimize the electroanalytical performance of the biosensor. The optimized biosensor on MWNTs-Chitosan-Urs modified GCE shows a linear current response to the urea concentration ranging from 30 to 610 μM and a sensitivity of 20.4 mA M-1cm-2 with a response time of about 25 s.
目錄
摘要 I
Abstract II
目錄 III
圖目錄 V
表目錄 VIII

第一章 序論 1
1-1 前言 1
1-2 分析物的簡介 3
1-2-1 分析物的簡介 3
1-2-2 分析物偵測之文獻回顧 5
1-3 生物感測器 10
1-3-1 生物感測器的定義 10
1-3-2 生物感測器的基本構造與組成 11
1-3-3 酵素的簡介 15
1-3-4 酵素動力學 16
1-3-5 酵素固定的方法 19
1-4 電化學方法 20
1-4-1 循環伏安法 20
1-4-2 線性掃描伏安法 23
1-4-3 安培法 24
1-5 奈米碳管 26
1-5-1 簡介 26
1-5-2 奈米碳管的特性 28
1-5-3 奈米碳管的應用 31
1-6 自組裝奈米薄膜 32
1-7 三氧化二鋁包覆二氧化矽奈米顆粒 33
1-8幾丁聚醣 34
1-8-1 幾丁聚醣的特性 34
1-8-2 幾丁聚醣的應用 35
1-8-3 幾丁聚醣在感測器上的應用 36
1-9 電子傳遞介質 37
第二章 實驗方法與步驟 38
2-1 實驗藥品 38
2-2 實驗儀器 38
2-3 實驗步驟 40
2-3-1 電極前處理 40
2-3-2 MWNTs-ACS複合薄膜修飾GCE 40
2-3-3 MWNTs-Chitosan-Urease生物複合薄膜修飾GCE 41
2-3-4 電化學測試 41
第三章 結果與討論 43
3-1 MWNTs-ACS複合薄膜修飾GCE偵測尼古丁 43
3-1-1 MWNTs-ACS微觀表面形貌之探討 43
3-1-2 MWNTs-ACS薄膜修飾GCE偵測尼古丁之試驗 45
3-2 MWNTs-Chitosan-Urs生物奈米複合薄膜修飾GCE偵測尿素 61
3-2-1 MWNTs-Chitosan-Urs生物奈米複合薄膜修飾GCE之表面形態分析 61
3-2-2 MWNTs-Chitosan-Urs生物奈米複合薄膜修飾GCE偵測尿素之試驗 64
第四章 結論與未來展望 79
4-1 結論 79
4-2 未來展望 79
第五章 參考文獻 81
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