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研究生:黃慧宜
研究生(外文):Hui-I Huang
論文名稱:芭菲爾鞋蘭之微體繁殖
論文名稱(外文):The Micropropagation of Paphiopedilum
指導教授:朱建鏞
指導教授(外文):Chien-Young Chu
學位類別:碩士
校院名稱:國立中興大學
系所名稱:園藝學系所
學門:農業科學學門
學類:園藝學類
論文種類:學術論文
畢業學年度:97
語文別:中文
論文頁數:68
中文關鍵詞:芭菲爾鞋蘭存活率培養基組成有機物
外文關鍵詞:Paphiopedilumsurvival ratecomposition of mediumorganic compound
相關次數:
  • 被引用被引用:11
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本試驗以芭菲爾鞋蘭雜交種(Paph. delenatii × Paph. armeniacum)已開過花之分植株營養系苗株作為試驗材料,成功地培養去葉植株之短縮莖,並從腋芽發育小苗。試驗中將培養材料在滅菌前先預處理乾燥2週,可提高成功率。而以含0.1% Tween 20之1% 次氯酸鈉溶液震盪滅菌9分鐘成活率較高。培養材料以選擇帶葉片5片者,成活率較高。另外培養基無生長調節劑者,初代培養之成功率可提高至66.7%。
MS培養基中CuSO4 .5H2O濃度提高至0.75 mg/l,可長出1個芽。在液體培養基中培養新增芽體數較在固體培養基中多,並可以減少根之形成。MS配方中H3BO3濃度降至原來的2/3,可促使芽體提早形成。
當MS培養基減去硝酸銨與硝酸鉀時,添加酵母萃取物取代氮源能新增3.67個芽體。雖然在高濃度(8或16 mg/l)的TDZ較低濃度者可促進芭菲爾鞋蘭芽體增生,但仍比添加BA 50 mg/l之效果低。此外,除葉處理可增加芽體之形成並抑制培植體長根。
The clonal plants of blossomed Paphiopedilum (Paph. delenatii × Paph. armeniacum) hybrids propagated by division were used as materials for initial culture. It succeeded to develop lateral shoots from the stem explants.When the material drought for two weeks before culture, the survival rate increased. To sterile by 10% NaOCl with 0.1% Tween 20 for 9 minutes resulted in higher survival rate. Also, material with five leaves resulted in higher survival rate. Moreover, explants cultured on hormone free medium enhanced the survival rate to 66.7%.
When the concentration of CuSO4 .5H2O in MS medium raised to 0.75 mg/l, explant sprouted 1 shoot. New shoots developed more shoots, but shorter roots from explants in liquid medium than that in solid medium. In the case of decreasing 1/3 the boron concentration of MS medium promoted shoots earlier.
When yeast extract as nitrogen source in the MS medium without NH4NO3 and KNO3, proliferated 3.67 shoots. Although explants on the medium containing higher concentration of TDZ (16 mg/l) proliferated more shoots than less one (8 mg/l ). The proliferated shoots were fewer than on medium containing BA 50 mg/l. In addition, explants excised leaves enhance new shoots production and inhibited rooting.
中文摘要..............................i
Summary...............................ii
前言..................................1
前人研究..............................2
  一、仙履蘭之性狀及分類............2
    (一)芭菲爾鞋蘭的分類..........2
    (二)產業現況..................4
  二、組織培養的前處理..............5
  三、培養基........................5
    (一)微量元素..................5
    (二)有機物....................6
    (三)細胞分裂素................7
    (四)活性碳....................8
    (五)液體培養..................8
  四、植體分析在組織培養上之應用....9
  五、仙履蘭的組織培養..............9
    (一)莖頂培養..................9
    (二)腋芽培養.................10
 (三)花梗芽培養...............10
    (四)癒傷組織培養.............10
    (五)子房培養.................11
材料與方法...........................12
結果.................................15
討論.................................20
參考文獻.............................63
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