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研究生:黃鈺倫
研究生(外文):Yu-Lun Huang
論文名稱:水稻台農67號香米突變品系香氣性狀之遺傳分析與基因定位
論文名稱(外文):Genetic analysis and mapping of aroma trait in aromatic mutant of TNG67 rice variety
指導教授:王強生
指導教授(外文):Chang-Sheng Wang
學位類別:碩士
校院名稱:國立中興大學
系所名稱:農藝學系所
學門:農業科學學門
學類:一般農業學類
論文種類:學術論文
畢業學年度:97
語文別:中文
論文頁數:78
中文關鍵詞:水稻香氣遺傳基因定位
外文關鍵詞:ricearomageneticsgene mapping
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  • 被引用被引用:4
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香米(fragrant rice; aromatic rice) (Oryza sativa L.)在世界上許多地區皆有栽種,因米粒帶有香氣而具有高附加價值,因此世界各地皆有育種家以香米作為育種目標,對於香米之香氣機制與遺傳也有許多研究。香米品種眾多,且香氣之成分相當複雜,因此對於香氣之確切遺傳模式仍未定論,目前大多數研究報告認為香氣為單一隱性基因所控制。本研究以水稻台農67號經由疊氮化鈉誘變之突變庫中SA0418、SA0420、SA0766、SA0766.1、SA1613及SA01613.1等香米突變體為材料,進行香氣遺傳研究;分別與TNG67及SA0420雜交產生F2族群,對SA0420×TNG67雜交組合730株F2植株的葉片進行香氣調查,發現葉片香氣香:不香的分離比呈現3:1 (χ2= 0.0164);因此,SA0420的香氣為單一完全顯性遺傳。將SA0420與其他五個香米突變品系雜交進行對偶性測驗,結果顯示,各香米突變品系之香氣基因均與SA0420不同,顯示各香米突變體之呈香可能由不同機制調控。本研究顯示疊氮化鈉誘變產生多種不同香米突變品系,有利於多樣性香米品種之育成,且能進一步探討香米呈香之分子機制。
本試驗將SA0420與台農67號雜交產生高變異性的F2族群,並持續自交育成具有香味歧異度的重組自交系 (recombinant inbred lines, RILs),建立簡單序列重複(SSR)的分子標誌系統進行香味基因之分析定位,並找尋與香味性狀相關之分子標誌,利用定位區域之SSR標誌進行分子標誌輔助選拔(marker-assisted selection, MAS),建立具有不同香味基因座之族群,以建立水稻香味品種選育之分子標誌輔助選拔系統。未來可將優良農藝性狀品種與香米突變品系或重組自交系雜交,透過分子標誌輔助選拔帶有香味基因座之品系,育成品質優良且帶有香氣之香米品種,增加水稻之食味與商品價值。
Aromatic rice (Oryza sativa L.) is highly priced in the world rice marketing. Breeding the aromatic rice variety and studying the inheritance and biochemical mechanism of aroma production are important to rice industry. The purpose of this thesis is to study the genetics and to map the aroma trait using the aromatic mutants including, SA0418, SA0420, SA0766, SA0766.1, SA1613 and SA1613.1 derived from seed sodium azide mutagenesis on Tainung 67 (TNG67) variety. The aroma trait of a novel aroma mutant, SA0420, is controlled by a single dominant and would be critical material in breeding aromatic rice variety. Allelism tests showed the aroma gene of SA0420 is different to those of other aromatic mutants. Various mechanisms and pathways may involve in producing aroma in the aromatic mutants of TNG67. The simple sequence repeat (SSR) system was established to map the aroma trait of SA0420 using 385 F8 recombinant inbred lines (RILs) derived from the cross of SA0420 X TNG67. The aroma trait of SA0420 mutant is mapped on the long arm of rice chromosome 8.
摘要 i
Abstract ii
目錄 iii
表目錄 iv
圖目錄 v
第一章 前言 1
第二章 前人研究 2
一、 香米品種香氣成分與呈香機制 2
二、 香米香氣之遺傳研究 4
三、 香米香氣基因定位 5
第三章 香米香氣判定與香米突變品系之遺傳分析 14
前言 14
材料與方法 15
結果 18
討論 20
第四章 香米香氣基因之定位與選殖 33
前言 33
材料與方法 35
結果 43
討論 47
參考文獻 75
周思儀。2004。利用蛋白質體技術探討水稻台農67號及其香米突變體SA0420之差異性蛋白質。碩士論文: pp 39-40。國立中興大學農藝學研究所,台中市,台灣。
劉宗華。2004。利用水稻為衛星分子標誌標定水稻台農67號突變體SA0420之香味基因。碩士論文: pp 38-39。國立中興大學農藝學研究所,台中市,台灣。
郭淑明。2005。水稻台農67號及其香米突變體SA0420香味相關基因之探討。碩士論文: pp 47-50。台中健康暨管理學院生物科技學系,台中縣,台灣。
楊遜謙、張萬來、趙政男、陳隆澤、陳一心。1990。稉稻香米品種台農72號之育成。中華農業研究。39:1-13。
賴明信、李長沛、曾清山、黃惠娟、陳治官、郭益全。2001。水稻台農71號(益全香米)的育成。中華農業研究。50:1-12。
Ahn, S.N., C. N. Bollich, and S.D. Tanksley. 1992. RFLP tagging of a gene for aroma in rice. Theor. Appl. Genet. 84:825-828.
Berner, D. K. and B. J. Hoff. 1986. Inheritance of scent in American long grain rice. Crop Sci. 26:876-878.
Bourgis, F., R. Guyot, H. Gherbi, E. Tailliez, I. Amabile, J. Salse, M. Loriux, M. Delseny, and A. Ghesquière. 2008. Characterization of the major fragrance gene from an aromatic japonica rice and analysis of its diversity in Asian cultivated rice. Theor. Appl. Genet. 117:353-368.
Bradbury, L. M. T., T. L. Fitzgerald, R. J. Henry, Q. Jin and D. L. E. Waters. 2005. The gene for fragrance in rice. Plant Biotechnol. J. 3:363-370.
Bradbury, L. M. T., S. A. Gillies, D. J. Brushett, D. L. E. Waters, and R. J. Henry. 2008. Inactivation of an aminoaldehyde dehydrogenase is responsible for fragrance in rice. Plant Mol. Biol. 68:439-449.
Buttery, R. G., L. C. Ling, B. O. Juliano, and J. G. Turnbaugh. 1983. Cooked rice aroma and 2-acetyl-1-pyrroline. J. Agric. Food Chem. 31:823-826.
Chen, S., J. Wu, Y. Yang, W. Shi, and M. Xu. 2006. The fgr gene responsible for rice fragrance was restricted within 69 kb. Plant Sci. 171:505-514.
Chen, S., Y. Yang, W. Shi, Q. Ji, F. He, Z. Zhang, Z. Cheng, X. Liu, and M. Xu. 2008. Badh2, encoding betaine aldehyde dehydrogenase, inhibits the biosynthesis of 2-acrtyl-1-pyrroline, a major component in rice fragrance. Plant Cell 20:1850-1861.
Chen, X., S. Temnykh, Y. Xu, Y. G. Cho, and S. R. McCouch. 1997. Development of a microsatellite framework map providing genome-wide coverage in rice (Oryza sativa L.). Theor. Appl. Genet. 95:553-567.
Cordeiro, G. M., M. J. Christopher, R. J. Henry and R. F. Reinke. 2002. Identification of microsatellite markers for fragrance in rice by analysis of the rice genome sequence. Molecular Breeding 9: 245-250.
Dhulappanavar, C. V. 1976. Inheritance of scent in rice. Euphytica 25:659-662.
Dong, Y., E. Tsuzuki, and H. Terao. 2001. Trisomic genetic analysis of aroma in three Japanese native rice varieties (Oryza sativa L.). Euphytica 117:191-196.
Fitzgerald, M. A., N. R. S. Hamilton, M. N. Calingacion, H. A. Verhoeven, and V. M. Butardo. 2008. Is there a second fragrance gene in rice? Plant Biotech. 6:416-423.
Fitzgerald, T. L., D. L. E. Waters, and R. J. Henry. 2008. The effect of salt on betaine aldehyde dehydrogenase transcript levels and 2-acetyl-1-pyrroline concentration in fragrant and non-fragrant rice (Oryza sativa). Plant Sci. 175:539-546.
Garland, S., L. Lewin, A. Blakeney, R. Reinke, and R. Henry. 2000. PCR-Based molecular markers for the fragrance gene in rice (Oryza sativa L.). Theor. Appl. Genet. 101:364-371.
Jain, N., S. Jain, N. Saini, and R. K. Jain. 2006. SSR analysis of chromosome 8 regions associated with aroma and cooked kernel elongation in Basmati rice. Euphytica.
Jin, Q., D. Waters, G. M. Cordeiro, R. J. Henry, and R. F. Reinke. 2003. A single nucleotide polymorphism (SNP) marker linked to the fragrance gene in rice (Oryza sativa L.). Plant Sci. 165:359-364.
Jodon, N.E. and E.A. Sonnier. 1973. Registration of Della Rice. Crop Sci. 13:773.
Laksanalamai, V. and S. Ilangantileke. 1993. Comparison of aroma compound (2-acetyl-1-pyrroline) in leaves from pandan (Pandanus amaryllifolius) and Thai fragrant rice (Khao Dawk Mali-105). Cereal Chem. 70:381-384.
Lanceras, J. C., Z. L. Huang, O. Naivikul, A. Vanavichit, V. Ruanjaichon, and S. Tragoonrung. 2000. Mapping of genes for cooking and eating qualities in Thai jasmine rice (KDML105). DNA research 7:93-101.
Lorieux, M., M. Petrov, N. Huang, E. Guiderdoni, and A. Ghesquiere. 1996. Aroma in rice: genetic analysis of a quantitative trait. Theor. Appl. Genet. 93:1145-1151.
McCouch, S.R., L. Teytelman, Y. Xu, K.B. Lobos, K. Clare, M. Walton, B. Fu, R. Maghirang, Z.K. Li, Y.Z. Xing, Q. Zhang, I. Kono, M. Yano, R.F. Jellstrom, G. DeClerck, D. Schneider, S. Cartinhour, D. Ware, and L. Stein. 2002. Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.). DNA Research 9: 199-207.
Meksem, K. and G. Kahl. 2005. The handbook of plant genome mapping: genetic and physical mapping. 1st ed. Weinheim. WILEY-VCH Verlag GmbH & Co. KGaA. pp.7-8.
Sun, S. X., F. Y. Gao, X. J. Lu, X. J. Wu, X. D. Wang, G. J. Ren, and H. Luo. 2008. Genetic analysis and gene fine mapping of aroma in rice (Oryza sativa L. Cyperales, Poaceae). Genet. Mol. Biol. 31:532-538.
Tripathi, R. S. and M. J. B. K. Rao. 1979. Inheritance and linkage relationship of scent in rice. Euphytica 28:319-323
Tsuzuki, E. and E. Shimokawa. 1990. Inheritance of aroma in rice. Euphytica 46: 157-159.
Wang, C. S., T. H. Tseng, and C. Y. Lin. 2002. Rice biotech research at the Taiwan Agriculture Research Institute. APBN. 6: 950-956.
Wanchana, S., W. Kamolsukyunyong, S. Ruengphayak, T. Toojinda, S. Tragoonrung, and A. Vanavichit. 2005. A rapid construction of a physical contig across a 4.5 cM region for rice grain aroma facilitates marker enrichment for positional cloning. Science Asia 31:299-306.
Yang, D. S., K. S. Lee, O. Y. Jeong, K. J. Kim, and S. J. Kays. 2008. Characterization of volatile aroma compounds in cooked black rice. J. Agric. Food Chem. 56:235-240.
Yoshihashi, T., N. T. T. Houng, and H. Inatomi. 2002.Precursors of 2-Acetyl-1-pyrroline, a potent flavor compound of an aromatic rice variety. J. Agric. Food Chem. 50:2001-2004.
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