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研究生:陳雅文
研究生(外文):Ya-wen Chen
論文名稱:表面電漿子增強螢光效應之研究
論文名稱(外文):Study of Surface Plasmon-Enhanced Fluorescence Effects
指導教授:陳顯禎
指導教授(外文):Shean-Jen Chen
學位類別:碩士
校院名稱:國立成功大學
系所名稱:工程科學系專班
學門:工程學門
學類:綜合工程學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:75
中文關鍵詞:螢光表面電漿子內全反射生命週期
外文關鍵詞:surface plasmonsfluorescencelifetimetotal internal reflection
相關次數:
  • 被引用被引用:3
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  • 下載下載:155
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利用表面電漿子共振(surface plasmons resonance,SPR)現象所研發的生物感測器已被廣泛研究及運用,而本論文主要藉由衰逝全反射(attenuated total reflection,ATR)方法來激發金屬薄膜中的表面電漿子(surface plasmons,SPs),經由區域電磁場的強化,來增強金屬膜上的螢光分子訊號。我們也藉由偵測螢光分子的生命週期變化來討論螢光訊號與金屬薄膜間之淬滅效應。
ATR方式如內全反射(total internal reflection,TIR)的方式一樣,光從高折射率的介質入射到低折射係數介質時,當入射光角度大於臨界角度並符合TIR時介面產生一種衰逝波(evanescent wave),此衰逝波可進而激發SPR,衰逝波為只存在於介面上約數百奈米內的表面波(surface wave),因此本論文探討100nm以下的不同厚度之介電層結構下表面電漿子增強螢光(surface plasmon-enhanced fluorescence,SPEF)的螢光強度訊號及螢光分子在金屬膜上會發生不同能量轉移的交互作用進而反應在生命週期上之效應。論文中製作SPEF感測器相較於內全反射螢光感測器約有2倍螢光增強之效應,而生命週期則隨介電層厚度增加而有上升之趨勢。
Biosensors based on surface plasmons resonance (SPR) have been extensively researched and applied. This thesis is focused to excite surface plasmons (SPs) on metal film by using attenuated total reflection (ATR) method to enhance the local electromagnetic field. With the filed enhancement, the fluorescence molecules on the metal film are excited and then their signal is improved. Also, the lifetime change of the fluorescence molecules is discussed and indicted to the quenching effect between the fluorescence signal and the metal film.
The ATR method similar to total internal reflection (TIR) method is to use light from high refractive index medium to low refractive index medium with the incident angle greater than critical angle. The light at the interface becomes into an evanescent wave, and the evanescent wave with a high k vector can excite SPs to achieve SPR when a metal thin film is inserted between the high and low refractive index media. The evanescent wave is a surface wave and only exists within few hundred nanometers from the interface. Therefore, this study is mainly to explore the factor of surface plasmon-enhanced fluorescence (SPEF) intensity with the different thickness of the dielectric layer between the metal and the fluoresce molecules. Furthermore, the excited fluorescence will transfer the fluorescent energy into the metal film to induce the change of the lifetime. The fluorescent sensor based on the SPEF can achieve the signal about 2-time enhancement compared to that of TIR fluorescence. The lifetime is increased when the thickness of the dielectric layer is increased.
摘要……………………………………………………………………Ⅰ
Abstract………………………………………………………………Ⅱ
誌謝……………………………………………………………………Ⅳ
目錄……………………………………………………………………Ⅴ
圖目錄…………………………………………………………………Ⅶ
表目錄…………………………………………………………………Ⅺ
第一章 序論……………………………………………………………1
1.1前言…………………………………………………………………1
1.2研究動機及目的……………………………………………………2
1.3文獻回顧……………………………………………………………3
1.4論文架構……………………………………………………………8
第二章 表面電漿子與螢光之基本原理………………………………9
2.1表面電漿子原理……………………………………………………9
2.1.1表面電漿子現象…………………………………………………9
2.1.2表面電漿子共振 ………………………………………………20
2.1.3表面電漿子激發 ………………………………………………22
2.2螢光 ………………………………………………………………29
2.2.1 螢光原理與特性………………………………………………29
2.2.2螢光生命週期 …………………………………………………31
2.3 表面電漿子於螢光增強…………………………………………35
第三章 表面電漿子增強螢光系統………………………………… 42
3.1 樣品製備…………………………………………………………42
3.1.1 濺鍍原理………………………………………………………42
3.1.2 金屬膜及介電膜製備…………………………………………46
3.2 光學系統架構……………………………………………………52
3.2.1 光路設計………………………………………………………52
3.2.2 螢光強度量測…………………………………………………54
3.3 結果與討論………………………………………………………58
第四章 螢光生命週期量測系統 ……………………………………59
4.1 光學系統架構……………………………………………………59
4.2 光路設計…………………………………………………………60
4.3 結果與討論………………………………………………………68
第五章 結論與未來工作 ……………………………………………69
參考文獻………………………………………………………………71
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