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研究生:嚴國維
研究生(外文):Kuo-wei Yen
論文名稱:以電紡絲法製備PLA/PHB芯鞘型纖維及其藥物釋放應用
論文名稱(外文):Preparation of PLA/PHB core/shell fibers via electrospinning and its application in drug release
指導教授:王紀
指導教授(外文):Chi Wang
學位類別:碩士
校院名稱:國立成功大學
系所名稱:化學工程學系碩博士班
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:121
中文關鍵詞:電紡絲芯鞘型纖維操作變數藥物釋放
外文關鍵詞:electrospinningprocessing variablesdrug releasecore/shell fiber
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本研究首先以PLA/DMF溶液,電紡出PLA奈米纖維,並探討在不同操做條件下,如:流量(Q)、 操作電壓、溶液黏度(η0)、溫度等對 cone、jet形態、液柱直徑(dj)及纖維直徑(df)的影響。實驗發現,改變流量與黏度,會對纖維外徑、液柱直徑造成影響,其sacling law的關係分別為dj~Q0.61、df~Q0.34 、dj~η00.07、 df~η00.45,而改變溫度則會使纖維直徑下降但程度有一限制。

在芯鞘型纖維方面以PHB/[CF+DMF]作為外管流體,PLA/DMF溶液作為內管流體,探討內管流體流量對電纺製程的影響,並利用SEM與FT-IR分析芯鞘型纖維之內徑;在藥物釋放實驗中,將藥物與電紡溶液相混,製備出不同條件的纖維膜,並利用紫外光/可見光光譜儀觀測其釋放速率。

改變內管流量(Qc)對芯鞘型纖維的外徑較無影響,但會使其內徑變大讓其纖維外層變薄,而內管流量(Qc)與液柱外徑(dj)及纖維內徑(df)、外徑(Df)的關係分別為:dj~Qc0.28、df~Qc0.18、Df~Qc0.02。

實驗結果顯示,將藥物置於不同條件的纖維內,可得到不同的釋放速率,若藥物加入纖維外層,釋放速率是first-order release,但若在纖維內層則其釋放速率會是與時間無關的zero-order release。
By the electrospinning process ,the PLA nano-fibers were prepared.The effects of the applied voltage、flow rate (Q) and solution viscosity (η0) on the Taylor cone, jet length, jet diameter (dj) , fiber diameter (df) were investigated. We also prepared core/shell fibers of PLA/PHB and discussed the effects of core flow rate(Qc) on the process of electrospinning. The scanning electron microscope(SEM) and Fourier transform infrared spectroscopy (FT-IR) were also used to analyze the inner fiber diameter(df);We also prepare the electrospun fibers containing added drug and the rates of drug release were determined by the UV/visible spectrometer.

The scaling laws obtained for the PLA/DMF electrospinning process between dj、df、Q and η0 are:dj ~Q0.61 、df~Q0.34 、dj ~ η00.07、df ~ η00.45 ; The relations between core fluid rate(Qc)、dj、df and Df are:dj~Qc0.28、df~Qc0.18、Df~Qc0.02.

The rate of drug release was first-order release when the drug was added in the shell fluid. If the drug was added in the core fluid , the release rate of drug was independent of time.
中文摘要 ………………………………………………………….. i
Abstract ………………………………………………………….. ii
致 謝 ………………………………………………………….. iii
目 錄 ………………………………………………………….. iv
表 目 錄 ………………………………………………………….. vii
圖 目 錄 ………………………………………………………….. viii
一、 前言…………………………………………………….. 1
二、 簡介…………………………………………………….. 2
2.1電紡絲模式………………………………………... 2
2.1.1 dripping mode………………………………. 2
2.1.2 pulsating mode………………………………. 2
2.1.3 cone-jet mode……………………………….. 2
2.1.4 multi-jet mode………………………………. 3
2.2電紡絲實驗之觀察………………………………..... 3
2.2.1 cone和jet之形態………………………….. 3
2.2.2 jet甩動之過程……………………………… 3
2.2.3纖維之形態………………………………… 4
三、 文獻回顧……………………………………………….. 9
3.1聚乳酸(PLA)與聚羥基丁酸酯(PHB)介紹………… 9
3.2 PLA之電紡絲…………………………………… 9
3.3 PHB之電紡絲…………………………………….... 10
3.4 coaxial 電紡絲……………………………………. 11
3.5 PLA、PHB相關之FT-IR鑑定與分析…………… 12
3.6藥物釋放機制與應用……………………………… 12
四、 實驗…………………………………………………….. 39
4.1實驗藥品…………………………………………… 39
4.2實驗材料及儀器…………………………………... 39
4.2.1 量測溶液性質之儀器……………………… 39
4.2.2 電紡絲儀器及材料………………………… 40
4.2.3 分析儀器…………………………………… 40
4.3溶液製備…………………………………………... 41
4.3.1聚乳酸電紡絲溶液配置……………………. 41
4.3.2聚羥基丁酸酯電紡絲溶液配置……………. 41
4.4 CF飽和蒸汽與氮氣的產生……………………….. 42
4.5電紡絲實驗步驟…………………………………... 43
4.6芯竅型纖維電紡絲實驗步驟………………………. 43
4.7芯竅型纖維斷面觀察步驟…………………………. 43
4.8藥物釋放實驗………………………………………. 43
4.9儀器操作步驟……………………………………… 44
4.9.1雷射量測步驟………………………………... 44
4.9.2 cone高度與jet長度之定義……………….. 44
五、 結果與討論…………………………………………….. 50
5.1溶液性質…………………………………………… 50
5.1.1 PLA/DMF溶液性質…………………………. 50
5.1.2 PHB/[CF/DMF]溶液性質……………………. 50
5.2電紡絲之變因探討…………………………………. 51
5.2.1電紡製備纖維………………………………... 51
5.2.2 PLA/DMF系統電紡可操作範圍……………. 51
5.2.3操作電壓對電紡絲的影響…………………... 52
5.2.4溶液流量對電紡絲之影響…………………... 52
5.2.5溶液黏度對電紡絲的影響…………………... 53
5.2.6溫度對電紡絲的影響……………………........ 55
5.3芯鞘型纖維製備……………………………………. 57
5.3.1內管流量對電紡絲之影響………………….... 57
5.3.2以FT-IR鑑定芯鞘型纖維膜之方法…………. 58
5.3.3以流量比與FT-IR預測內管直徑…………… 58
5.3.4 WAXD與DSC測試………………………….. 60
5.3.5 內管流量對纖維膜內部DMF含量的影響…. 61
5.4 DMOG於電紡纖維中的釋放速率………………… 62
六、 結論……………………………………………….......... 117
七、 參考文獻……………………………………………….. 118
八、 自述…………………………………………………….. 121
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