跳到主要內容

臺灣博碩士論文加值系統

(18.207.132.116) 您好!臺灣時間:2021/07/29 20:02
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

: 
twitterline
研究生:邱宜亭
研究生(外文):Yi-ting Chiu
論文名稱:高錳酸鉀對三種藍綠菌破壞及其代謝物釋出之研究
論文名稱(外文):Effect of Permanganation on the Cell Integrity and Metabolite Release of Three Nauseous Cyanobacteria
指導教授:林財富林財富引用關係
指導教授(外文):Tsair-Fuh Lin
學位類別:碩士
校院名稱:國立成功大學
系所名稱:環境工程學系碩博士班
學門:工程學門
學類:環境工程學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:127
中文關鍵詞:β-cyclocitral細胞完整性geosmin流式細胞儀高錳酸鉀氧化作用
外文關鍵詞:β-cyclocitralgeosmincell integrityflow cytometerpermenganation
相關次數:
  • 被引用被引用:7
  • 點閱點閱:393
  • 評分評分:
  • 下載下載:73
  • 收藏至我的研究室書目清單書目收藏:0
本研究主要探討當藍綠菌細胞受到高錳酸鉀破壞後,細胞破損的程度與其胞內代謝物釋出之相關性。實驗以高錳酸鉀作為氧化劑,並以自來水水源中三種常見產臭或毒之藍綠菌作為研究對象,包括微囊藻(Microcystis aeruginosa) 、魚腥藻(Anabaena circinalis) , 及柱孢藻(Cylindrospermopsis raciborski) , 所使用之微囊藻具有產高濃度β-cyclocitral 的能力,魚腥藻會產生geosmin,而柱孢藻所產生之柱孢藻毒則還未對其進行偵測。實驗中使用之水分成兩大部分:第一部份以培養基為背景液進行氧化實驗;第二部分則以經數道過濾之金門太湖原水與培養基混合之合成水。
研究中藍綠菌細胞被高錳酸鉀所破壞的程度,以細胞完整性來作表示,並應用螢光染劑FDA(fluorescein diacetate)搭配流式細胞儀(flow cytometer, FCM),測試其完整程度,並應用掃描式電子顯微鏡(scanning electron microscope, SEM)對細胞進行高放大倍率的觀察,以協助判定細胞表面完整情形。在代謝物分析部分,研究中以β-cyclocitral、geosmin 分別做為微囊藻與魚腥藻代謝物的代表,並應用固相微萃取法(solid phase micro-extraction, SPME) 搭配氣相層析質譜儀(gas chromatograph/mass spectrometry detector, GC/MSD)對此兩種臭味物質進行分析。
研究結果發現柱孢藻對於高錳酸鉀的抗氧化力低於微囊藻。微囊藻及柱孢藻之氧化實驗中,於藻數300,000 cells/mL、高錳酸鉀2 mg/L及氧化60分鐘下,背景為培養基時,微囊藻約有57%的細胞受到破壞,而柱孢藻則有74%的細胞受到破壞。當背景改為金門太湖原水時,微囊藻只有15%的細胞受到破壞。
在代謝物方面,當微囊藻受到一定的破壞時,高錳酸鉀便可進入細胞與胞內之β-carotene氧化,斷鍵形成β-cyclocitral,使得總β-cyclocitral的濃度有上升的情形產生,然而當氧化時間延長至120分鐘時,降解作用會大於β-cyclocitral的生成,因此使得總β-cyclocitral的濃度會呈現降低的現象。而於魚腥藻代謝物geosmin偵測上,魚腥藻細胞的受損,可能增加了合成酶反應中Mg2+的濃度,因而增加反應速率,並於短時間內呈現geosmin增加之趨勢,且geosmin的揮發性及抗氧化性使得geosmin的濃度於氧化過程中呈現些微的減少,甚至是穩定且持平的狀態。
The effect of permanganation on the cell integrity and metabolite release of three nauseous cyanobacteria, including Microcystis aeruginosa, Anabaena circinalis, and Cylindrospermopsis raciborskii, is investigated. In the experiment, the cyanobacteria were grown either in algae growth medium or in the filtrated water from Tai-Lake, Kinmen, with addition of algae growth medium. A fluorescence technique, combining fluorescein diacetate (FDA) with flow cytometer, was successfully developed for the determination of cell integrity for M. aeruginosa and C. raciborskii, while was not successful for A. circinalis. A solid-phade microextraction (SPME) concentration followed by a gas chromatograph (GC) and mass spectrometric detector (MSD) was employed to measure the metabolites from the cyanobacteria. A series of permangnation of cyanobacteria-laden water was conducted at different cell concentrations and different permangnate dosages. During the experiments, permanganate concentration, cell integrity, and metabolite concentration were monitored at different time.
The experimental results revealed that the Cylindrospermopsis cells are more fragile to permanganate than Microcystis cells. At an initial permanganate concentration of 2 mg/L, cells number of 300,000 cells/mL, and reaction time of 60 minutes, 57% Microcystis cells, and 74% Cylindrospermopsis cells were ruptured in algae growth medium, and only 15% Microcystis cells were ruptured in the filtrated water from Tai-Lake.
For Microcystis metabolite, both β-carotene oxygenases present in the cells and permanganate may be able to cleave the carbon-carbon double bonds of β-carotene, resulting in the formation of β-cyclocitral, Since β-cyclocitral may be degraded by permanganate, for longer reacton time (120 minutes in this case), the rate of degradation may become higher than that of formation, casuing a reduction of β-cyclocitral concentration in the samples. For geosmin, the major nauseous metabolite of Anabaena, may be formed by the geosmin sythetases in the cells with magnesium as catalyst. When Anabaena cells are ruptured, magnesium would have more chances to contact with the geosmin synthetases, resulting in the formation of geosmin. Since geosmin is resistant to permanganate, the total geomsin concentration in the samples did not change much in the permangnation experiments of Anabaena.
摘要 I
Abstract III
致謝 V
目錄 VII
表目錄 X
圖目錄 XI

第一章 緒論 1
1-1 研究緣起 1
1-2 研究目的 3
第二章 文獻回顧 5
2-1 藍綠細菌代謝物之來源、種類與化學結構 5
2-1-1 藍綠細菌毒素 5
2-1-2 藍綠細菌臭味 9
2-2 氧化作用對於藍綠細菌代謝物釋出之影響 17
2-2-1 氧化作用對於藍綠菌體的破壞 17
2-2-2 高錳酸鉀之物化特性 18
2-2-3 高錳酸鉀之反應作用 19
2-3 螢光染色應用於藻體觀測 23
2-3-1 螢光染劑 24
2-3-2 流式細胞儀 27
2-3-3 螢光顯微鏡 29
第三章 實驗設備與方法 31
3-1 高錳酸鉀氧化實驗方法 32
3-1-1 氧化實驗藻體之準備 32
3-1-2 氧化實驗 32
3-2 藍綠菌培養 35
3-2-1 藍綠菌之來源 35
3-2-2 藍綠菌之培養方法 36
3-3 藍綠菌計數 38
3-3-1 細胞計數盤 38
3-3-2 微囊藻及魚腥藻計數 40
3-3-3 柱孢藻計數 40
3-4 藍綠菌細胞完整性觀察 41
3-4-1 流式細胞儀 41
3-4-2 掃描式電子顯微鏡 42
3-5 臭味物質分析 44
第四章 結果與討論 47
4-1 螢光顯微鏡與流式細胞儀實驗測試 47
4-1-1 螢光顯微鏡可行性之測試 47
4-1-2 流式細胞儀可行性之測試 48
4-1-3 高錳酸鉀對細胞完整性觀察之影響 50
4-2 高錳酸鉀對微囊藻之氧化結果 59
4-2-1 微囊藻藻數100,000 cells/mL 59
4-2-2 微囊藻藻數300,000 cells/mL 62
4-2-3 微囊藻藻數700,000 cells/mL 64
4-2-4 微囊藻藻數300,000 cells/mL於過濾藻體之太湖原水 68
4-2-5 高錳酸鉀氧化微囊藻動力模式之探討 72
4-2-6 微囊藻細胞經氧化後SEM 觀測的結果 76
4-2-7 小結 79
4-3 高錳酸鉀對魚腥藻之氧化結果 80
4-3-1 魚腥藻藻數100,000 cells/mL 80
4-3-2 魚腥藻藻數300,000 cells/mL 83
4-3-3 魚腥藻藻數1,500,000 cells/mL 89
4-3-4 魚腥藻藻數300,000 cells/mL於過濾藻體之太湖原水 91
4-3-5 高錳酸鉀氧化魚腥藻動力模式之探討 95
4-3-6 魚腥藻細胞經氧化後SEM觀測的結果 97
4-3-7 小結 100
4-4 高錳酸鉀對柱孢藻之氧化結果 101
4-4-1 柱孢藻藻數 100,000 cells/mL 101
4-4-2 柱孢藻藻數 300,000 cells/mL 102
4-4-3 高錳酸鉀氧化柱孢藻動力模式之探討 104
4-4-4 柱孢藻細胞經氧化後SEM觀測的結果 107
4-4-5 小結 111
第五章 結論與建議 113
5-1 結論 113
5-2 建議 115

參考文獻 117
自述 127
Akhlaq, M.S., Schuchann, H.P., and von Sonntag, C., (1990), Degradation of the polysaccharide alginic acid: A comparson of the effect of UV light and ozone, Environ. Sci. Technol., Vol. 24, pp. 379-383.
Arana, I., Santorum, P., Muela, A. and Barcina, I., (1999), Chlorination andozonation of waste-water: comparative analysis of efficacy throuth the effecton Escherichia coli membranes. Journal of Applied Microbiology, No. 86, pp. 883-888.
Ashitani, K., Hishida, Y., and Fujiwara, K., (1988), Behavior of musty odorous compounds during the process of water treatment, Water Science & Technology, Vol. 20, No. 8/9, pp. 261-267.
Bellar, T.A., Lichetngberg, J.J., and Kroner,R.C., (1974), The occurance of organohalides in chlorinated drinking water, Journal AWWA, Vol. 66, pp. 703-706.
Bentley, R., and Meganathan, R., (1981), Geosmin and methylisoborneol biosynthese in streptomycetes. Evidence for an isoprenoid pathway and its absence in non-differentiating isolates, FEBS Lett., Vol. 125, pp. 259-267.
Berg, K., Carmichael, W.W., Skulberg, O.M., Benested, C. and Underlal, B. (1987), Investigation of a toxin water bloom of Microcystis aeruginosa (cyanophyceae) in lake Akersvatn, Norway, Hydrobiologia, Vol. 144, pp. 97-103.
Boulos, L., Prevost, B., Goalier, J., and Desjardins, R., (1999), Live / Dead BacLightTM : application of a new rapid staining method for direct enumeration of viable and total bacteria in drinking water. Journal of Microbiological Method, Vol. 37, pp. 77-86.
Breeuwer, P., Drocourt, J.L., Bunschoten, N., Zwietering, M.H., Rombouts, F.M., and Abee, T., (1995), Characterization of uptake and hydrolysis of fluorescein diacetate and carboxyfulorescein diacetate by intracellular exterases in saccharomyces-cerevisiae, which result in accumulation of fluorescent product, Applied and Environmental Microbiology, Vol. 61, No. 4, pp. 1614-1619.
Bruchet, A., Duguet, J.P., and Suffet, I.H., (2004), Role of oxidants and disinfectants on the removal, masking and generation of tastes and odours, Re/view in Environmental Science & Bio/Technology, Vol. 3, pp. 33-41.
Budavari, S., O'Neil, M.J., Smith, A., and Heckelman, P.E., (1989), 7643: Potassium permanganate, The Merck Index, 11th edition, Merck &Co., Inc., 7636.
Cane, D.E., He, X., Kobayashi, S., Omura, S., and Ikeda, H., (2006), Geosmin biosynthesis in Streptomyces avermitilis. Molecular cloning, expression, and mechanistic study of the germacradienol/geosmin synthase, J. Antibiotics, Vol. 59, pp. 471-479.
Carmichael, W.W., Eschedor, J.T., Patterson, G.M.L. and Moore, R.E. (1988), Toxicity and partial structure of a hepatotoxic peptide produced by the cyanobacterium Nodularia spumigena Mertens emend, L575 from New Zealand, Applied and Environmental Microbiology, Vol. 54, pp. 2257-2263.
Chen, J.-J., Yeh, H.-H., (2005), The mechanisms of potassium permanganate on algae removal, Water Research, No. 39, pp. 4420-4428.
Chorus, I., and Bartram, J., (1999), Toxic cyanobacteria in water, E&FN Spon on behalf of WHO (editors).
Chow, C.W.K., House, J., Velzeboer, R.M.A., Drikas, M., Burch, M.D., and Steffensen, D.A., (1998), The effect of ferric chloride flocculation on cyanobacterial cells, Water Research, No. 32, pp. 808-814.
Chow, C.W.K., Drikas, M., House, J., Burch, M.D., and Velzeboer, R.M.A., (1999), The impact of conventional water treatment processes on cells of the cyanobacterium Microcystis aeruginosa, Water Research, No. 33, pp. 3253-3262.
Coleman, A.W., (1980), Enhanced detection of bacteria in natural environments by fluorochrome staining of DNA, Limnol. Oceanogr, Vol. 25, pp. 948-951.
Daley, R.J., Hobbie, J.E., (1975), Direct counts of aquatic bacteria by a modified epifluorescence technique. Limnol. Oceanorg, No. 20, pp. 875-882.
Daly, R.I., Ho, L., and Brookes, J.D., (2007), Effect of chlorination on microcystis aeruginosa cell integrity and subsequent microcys tin release and degradation, Environ. Sci. Technol., No. 41, pp. 4447-4453.
Delgiorgio, P.A., Prairie, Y.T., and Bird, D.F., (1997), Coupling between ratesof bacterial production and the abundance of metabolically active bacteria inlakes, enumerated using CTC reduction and flow cytometry, Microb. Ecol., Vol. 34, pp. 144-154.
Diaper, J.P., Tither, K., and Edwards, C., (1992), Rapid assessment of bacterial viability by flow-cytometry, Applied Microbiology and Biotechnology, Vol. 38, No. 2, pp. 268-272.
Dietrich, M. and Hoehn, R.C., (1995), Oxidation of odorous and nonodorous algal metabolites by permanganate, chlorine, and chlorine dioxide., Water Science Technology, Vol. 31, No. 11, pp. 223-228.
Dorsey, J., Yentsch, C.M., Mayo, S., and McKenna, C., (1989), Rapid analytical technique for the assessment of cell metabolic-activity in marine microalgae, Cytometry, Vol. 10, No. 5, pp. 622-628.
Drikas, M., Chow, C.W.K., House, J., and Burch, M.D., (2001), Using coagulation, flocculation, and settling to remove toxic cyanobacteria, J. Am. Water Works Assoc., Vol. 93, No. 2, pp. 100-111.
Drikas, M., Newcombe, G., and Nicholson, B., (2002), Water treatment options for cyanobacteria and their toxins, In Blue-Green Algae: Their significance and management within water supplies, the cooperative research centre for water quality and treatment, occasional paper 4.
Durrer, M., Zimmermann, U., and Jüttner, F., (1999), Dissolved and particle-bound geosmin in a mesotrophic lake (Lake Zürich): spatial and seasonal distribution and the effect of grazers, Water Research, Vol. 33, pp. 3628-3636.
Falconer, I.R., (1996), Potential impact on human health of toxic cyanobacteria, Phycologia, Vol. 35, pp. 6-11.
Fastner, J., Heinze, R. and Chorus, I. (1995), Microcystin-content, hepatotoxicity and cytotoxicity of cyanobacteria in some german water bodies, Water Science Technology, Vol. 32, No. 4, pp. 165-170.
Ficek, K.J. (1980), Chap. 21: Potassium permanganate for iron and manganese removal and taste and odor control, Water Treatment Plant Densign, R. L Sanks (editor), Ann Arbor Science Publishers, Inc., pp. 461-479.
Ficek, K.J. and Reidies, A.H. (1992), Chap. 8: Potassium permanganate, Disinfection Alternatives for safe Drinking Water, Bryant, E. A., Fulton, G. P., and Budd, G. C.(editor), NewYork: Van Nostrand Rcinhold, pp. 259-276.
Gerber, N.N., (1969), A volatile metabolite of actinomycetes 2-methylisoborneol, Journal of Antibiotics, Vol. 22, pp. 508-509.
Giglio, S., Jiang, J., Saint, C.P., Cane, D.E., and Monis, P.T., (2008), Isolation and characterization of the gene associated with geosmin production in cyanobacteria, Environmental Science & Techmology, Vol. 42, No. 21, pp. 8027-8032.
Glaze, W.H., (1990), Evaluating oxidants for the removal of model taste and odor compounds from a municipal water supply, Jour. AWWA, No. 82, pp. 79-84.
Grosse, Y., Baan, R., Straif, K., Secretan, B., El Ghissassi, F., and Cogliano, V., (2006), Carcinogenicity of nitrate, nitrite, and cyanobacterial peptide toxins, Oncology, Vol. 7, pp. 628-629.
Haider, S., Naithani, V., Viswanathan, P.N. and Kakkar, P., (2003), Cyanobacterial toxins: a growing environmental concern, Chemosphere, Vol. 52, pp. 1-21.
Hall T., Hart, J., Croll, B., and Gregory, R., (2000), Laboratory-scale investigations of algal toxin removal by water treatment, J. Ciwem, Vol. 14, pp. 143-149.
Hayes, K.P. and Burch, M.D., (1989), Odorous compounds associated with algal blooms in south Australian waters., Water Res., Vol. 23, No. 1, pp. 115-121.
Hernández, M., Macia, M., Padilla, C. and Campo, F.F.D., (2000), Short Communication: modulation of human polymorphonuclear leukocyte adherence by cyanopeptide toxins, Environmental Research, Vol. A84, pp. 64-68.
Hobbie, J.E., Dayley, R.J., and Jasper, S., (1977), Use of nucleopore filters for counting bacteria by fluorescence microscopy, Appl. Environ. Microbiol., Vol. 33, pp. 1225-1228.
Hofstraat, J.W., W.J.M. van Zeijl, Peeters, J.C.H., Peperzak, and Dubelaar, G.B.J., (1990), Flow cytometry and other optical methods for characterization and quantification of phytoplankton in seawater. Environment and pollution measurement sensors and systems, Vol. 1269, pp. 116-132.
Hummert, C., Dahlmann, J., Reichelt, M., and Luckas, M., (2001), Analytical techniques for monitoring harmful cyanobacteria in lakes, Lakes and Reservoirs: Research and Management, Vol. 6, pp. 159-168.
Hummert, C., Reichelt, M., Weiβ, J., Liebert, H., and Luckas, B., (2001), Identification of microcystins in cyanobacteria from the Bleiloch former drinking-water reservoir (Thuringia Germany), Chemosphere, Vol. 44, pp. 1581-1588.
Izaguirre, G., Hwang, C.J., Krasner, S.W., and McGuire, M.J., (1982), Geosmin and 2-methylisoborneol from cyanobacteria in three water supply systems, Applied and Environmental Microbiology, Vol. 43, No. 3, pp. 708-714.
Izaguirre, G., and Taylor W.D., (2007), Geosmin and MIB events in a new reservoir in southern California., Water Science Technology, Vol. 55, No. 5, pp. 9-14.
Jiang, J., and Cane, D.E., (2008), Geosmin biosynthesis. Mechanism of the fragmentation- rearrangement in the conversion of germacradienol to geosmin, Journal of the American Chemical Society, Vol. 130, pp. 428-429.
Jones, G.J., and Korth, W., (1995), In situ production of volatile odor compounds by river and reservoir phytoplankton populations in Australia., Water Science Technology, Vol. 31, No. 11, pp. 145-151.
Jüttner, F., (1976), β-cyclocitral and alkanes in Microcystis (Cyanophyceae), Zeitschrift fur Naturforschungl, Vol. 31, pp. 491-495.
Jüttner, F., (1984), Dynamics of the Volatile organic substances associated with cyanobacteria and algae in a eutrophic shallow lake., Applied and Environmental Microbiology, Apr, pp. 814-820.
Jüttner, F., and Höflacher, B., (1985), Evidence of β-carotene 7,8 (7',8') oxygenase (β-cyclocitral, crocetindial generating) in Microcystis, Archives of Microbiology, Vol. 141, pp. 337-343.
Landsgrud, S., and Sundheim, G., (1996), Flow cytometry for rapid assessment of viability after exposure to a quarternary am-monium compound, J. Appl. Bacteriol., Vol. 81, pp. 411-418.
Li, W.K.W., (1997), Cytometric diversity in marine ultraphytoplankton, Limnol. Oceanogr., 42(5), pp. 874-880.
Li, W.K.W., and Wood, A.M., (1988), Vertical distribution of northern atlantic ultraphytoplankton analysis by flow cytometry and epifulorescence microscopy. Deep-sea Res. Part A Oceanogr. Res. Pap., Vol. 35, No. 9, pp. 1615-1638.
Kenneth, H.J., and Senft, J.A., (1985), An improved method to determine cell viability by simultaneous staining with fluorescein diacetate-propidium iodide. The Journal of Histochemistry and Cytochemistry, Vol. 33, No. 1, pp. 77-79.
Knappe, R., Detlef, R. U., Belk, C., Briley, D. S., Grandy, S. R., Rastogi, N., and Rike, A. H., (2004), Algae Detection and Removal Strategies for Drinking Water Treatment Plants. AWWA Research Foundation, Denver, USA.
Krasner, S.W., Hwang, C.J., and McGuire, M.J., (1983), A standard method for quantification of earthy-musty odorants in water, sediments and algae cultures., Water Sci. Techno., Vol. 15, pp. 127-138.
Kull, T.P.J., Backlund, P.H., Karlsson, K.M., and Meriluoto, J.A.O., (2004), Oxidation of the cyanobacterial hepatotoxin microcystin-LR by chlorine dioxide: reaction kinetics, characterization and toxicity of reaction products. Environ. Sci. Technol., Vol. 38, pp. 6025-6031.
Kuzuyama, T., (2002), Mevalonate and nonmevalonate pathways for the biosynthesis of isoprene units, Biosci. Biotechnol. Biochem., Vol.66, pp. 1619-1627.
Lalezary, S., Pirbazari, M., and McGuire, M. J., (1986), Oxidation of five earthy-musty taste and odor compounds, Jour. AWWA., Vol. 78, pp. 62-69.
Liu, I., Lawton, L.A. and Robertson, K.J., (2003), Mechanistic studies of the photocatalytic oxidation of microcystin-LR: an investigation of byproducts of the decomposition process. Environ. Sci. Technol., Vol. 37, pp. 3214-3219.
Ma, J., Graham, N. and Li, G., (1997), Effect of permanganate preoxidation in enhancing the coagulation of surface water-laboratory case studies, Water Supply Research Technology-Aqua, Vol. 46, No. 1, pp. 1-10.
Maatouk, I., Bouaicha, N., Fontan, D., and Levi, Y., (2002), Seasonal variation of microcystin concentrations in the saint-caprais reservoir (France) and their removal in a small full-scale treatment plant, Water Research, Vol. 36, pp. 2891-2897.
Marie, D., Vaulot, D., and Partensky, F., (1996), Application of the novelnucleic acid dyes YOYO-1, YO-PRO-1, and PicoGreen for flow cytometricanalysis of marine prokaryotes, Applied and Environmental Microbiology, Vol. 62, pp. 1649-1655.
Maizels, M. and Budde, W.L. (2004), A LC/MS Method for the Determination of Cyanobacteria Toxins in Water, Analysis Chemistry, Vol. 76, pp. 1342-1351.
Mc Feters, G.A., Yu, F.P., Pyle, B.H., and Stewart, P.S., (1995), Physiological assessment of bacteria using fluorochromes. J. Microbiology Method, No. 21, pp. 1-13.
Moffat, B.D., and T.W. Snell, (1995), Rapid toxicity assessment using an in-vivo enzyme test for brachionus-plicatilis (rotifera), Ecotoxicology and Environmental Safety, Vol. 30, No. 1, pp. 47-53.
Montiel, A., and Welte, B., (1998), Preozonation coupled with flotation filtration: Successful removal of algae. Water Science & Technology, Vol. 37, pp. 65-73.
Moreira-Turcq, P.F., and Martin, J.M., (1998), Characterisation of fine particles by flow cytometry in estuarine and coastal Arctic waters, J. Sea Research, Vol. 39, No. 3/4, pp. 217-226.
Moyers, B. and Wu, Y. S., (1985), Remocal of organic precursors by permanganate oxidation and alum coagulation, Water Research, Vol. 19, No. 3, pp. 309-314.
Nawrath, T., Dickschat, J.S., Muller, R., Jiang, J., Cane, D.E., and Schulz, S., (2008), Identification of (8S,9S,10S)-8,10-dimethyl-1-octalin, a key intermediate in the biosynthesis of geosmin in bacteria, Journal of the American Chemical Society, Vol. 130, pp. 430-431.
Nicholson, B.C., Rositano, J., and Burch, M.D., (1994), Destruction of cyanobacterial peptide hepatotoxins by chlorine and chloramines, Water Research, Vol. 28, pp. 1297-1303.
Onstad, G., Strauch, S., Meriluoto, J., Codd, G. A., and Gunten, U., (2006), Selective oxidation of key functional groups in cyanotoxins during drinking water ozonation. Environ Sci Technol. Submitted for publication.
Persson, P.E., (1980), Sensory properties and analysis of two muddy odour compounds, geosmin and 2-methylisoborneol, in water and fish., Water Research, Vol. 14, pp. 1113-1118.
Persson, P.E., (1983), Off-flavor in aquatic ecosystems-An introduction, Water Science & Technology, Vol. 15, No. 1, pp. 2.
Persson, P.E., (1988), Odorous algal cultures in culture collections, Water Science & Technology, Vol. 20, pp. 211-213.
Petruevski, B., Van Breemen, A. N. and Alaerts, G., (1996), Effect of permanganate pre-treatment and coagulation with dual coagulants on algae removal in direct filtration., J. Water Supply Research Technology-Aqua, Vol. 45, No. 5, pp. 316-326.
Pietsch, J., Bornmann, K., and Schmidt, W., (2002), Relevance of intraand extracellular cyanotoxins for drinking water treatment, Acta Hydrochim Hydrobiol., Vol. 30, pp. 7-15.
Plummer, J.D., and Edzwald, J.K., (2002), Effects of chlorine and ozone on algal cell properties and removal by coagulation, J. Water Supply Research Technology-Aqua, Vol. 51, pp. 307-318.
Porter, K.G., and Feig, Y.C., (1980), The use of DAPI for identifying and counting aquatic microflora. Limnol. Oceanogr, No. 25, pp. 943-948.
Pyo, D., and Shin, H., (2002), Extraction and analysis of microcystins RR and LR in cyanobacteria using a cyano cartridge, Journal of Biochemical and Biophysical Methods, Vol. 51, pp. 103-109.
Queric, N.V., Soltwedel, T., and Arntz, W.E., (2004), Application of a rapid direct viable count method to deep-sea sediment bacteria, Microbiology Method, Vol. 57, No. 3, pp. 351-367.
Rodríguez-Conceptión, M., and Boronat, M., (2002), Elucidation of the methylerythritol phosphate pathway for isoprenoid biosynthesis in bacteria and plastids. A metabolic milestone achieved through genomics, Plant Physiology, Vol. 130, pp. 1079-1089.
Rodríguez, E., Majado, M.E., Meriluoto, J., and Acero, J.L., (2007a), Oxidation of microcystins by permanganate: Reaction kinetics and implications for water treatment, Water Reaearch, Vol. 41, pp. 102-110.
Rodríguez, E.B., and Rodríguez-Amaya, D.B., (2007b), Formation of apocarotenals and epoxycarotenoids from b-carotene by chemical reactions and by autoxidation in model systems and processed foods, Food Chemistry, Vol. 101, pp. 563-572.
Rositano, J., Newcombe, G., Nicholson, B., and Sztajnbok, P., (2001), Ozonation of NOM and algal toxins in four treated waters, Water Research, Vol. 35, pp. 23-32.
Rositano, J., (1996), The destruction of cyanobacterial peptide toxins by oxidants used in water treatment, Urban Water Research Association of Australia, Report 110.
Rouhiainen, L., Vakkilainen, T., Siemer, B.L., Buikema, W., Haselkorn, R., and Sivonen, K., (2004), Genes Coding for Hepatotoxic Heptapeptides (Microcystins) in the Cyanobacterium Anabaena Strain 90, Applied and Environmental Microbiology, Vol. 70, No. 2, pp. 686-692.
Skoog, D.A., West, D.M., and Holler, F.J., (1990), Chap. 17: Applications of oxidation / reduction titrations, Analytical Chemistry: An Intriduction, 5th edition, Yi Hsien Publishing Co., LTD.
Slater, G.P., and Block, V.C., (1983), Volatile compounds of the cyanophyceae-a review, Water Science & Technology, Vol. 15, No. 6/7, pp. 181-190.
Solange Duhamel, and Ste´phan Jacquet, (2006), Flow cytometric analysis of bacteria- and virus-like particles in lake sediments, Journal of Microbiological Methods, Vol. 64, pp. 316-332.
Spiteller, D., Jux, A., Piel, J., and Boland, W., (2002), Feeding of [5,4-2H2]-1-desoxy-d-xylulose and [4,4,6,6,6-2H5]-mevalolactone to a geosmin-producing Streptomyces sp. and Fossombronia pusilla, Phytochemistry, Vol. 61, pp. 827-834.
Suffet, I.H., Khiari, D., and Bruchet, A., (1999), The drinking water taste and odor wheel for themillenium: Beyond geosmin and 2-methylisoborneol, Water Science & Technology, Vol. 40, No. 6, pp. 1-13.
Thomas, H.C., Rhonda, D.C., James, G.H., and Robbert, P.W., (1984), Applicability of the fluorescein diactate method of detecting active bacteria in fresh water, Micro. Ecol., Vol. 10, pp 179-185.
USEPA (2001), Creating a Cyanotoxin Target List for the Unregulated Contaminant Monitoring Rule.
Vasas, G., Gaspar, A., Pager, C., Suranyi, G., Mathe, C., Hamvas, M.M., and Borbely, G., (2004), Analisis of cyanobacterial toxins (anatoxin-a, cylindrospermopsin, microcystin-LR) by capillary electrophoresis, Electrophoresis, Vol. 25, pp. 108-115.
Veldhuis, M.J.W., Cucci, T.L., Sieracki, M.E., (1997), Cellular DNA content of marine phytoplankton using two new fluorochromes: Taxonomic and ecological implications, Journal of Phycology, Vol. 33, No. 3, pp. 527-541.
Velzeboer, R., Drikas, M., Donati, C., Burch, M., and Steffensen, D., (1995), Release of geosmin by Anabaena circinalis following treatment with aluminium sulphate, Water Science & Technology, Vol. 31, pp. 187-194.
Watson, S.B., (2004), Aquatic taste and odor: A pimary signal of drinking-water integrity, Journal of Taxicology and Environmental Health, part A, Vol. 67, pp. 1779-1795.
Weinbauer, M.G., Beckmann, C., and Hofle, M.G., (1998), Utility of green fluorescent nucleic acid dyes and aluminium oxide membrane filters for rapid epifluorescence enumeration of soil and sediment bacteria, Applied and Environmental Microbiology, No. 64, pp. 5000-5003.
Wu, J.T., and Jüttner, F., (1988), Differential partitioning of geosmin and 2-methylisoborneol between cellular constituents in Oscillatoria tenuis, Arch. Microbiol., Vol. 150, pp. 580-583.
Wu, J.T., and Jüttner, F., (1988), Effect of environmental factors on geosmin production by Fischerella muscicola, Water Sci. Technol., Vol. 20, pp. 143-148.
Yen, H.K., Lin, T.F., Tseng, I.C., Tung, S.C., Hsu, M.H., and Liao, P.C., (2004), Occurrence of Algal Toxins and Odorants in Two Reservoirs in South Taiwan, The 10th International Drinking Water Quality Management and Treatment Technology, Taipei, Taiwan.
Yentsch, C.S., and C.M. Yentsch, (1979), Fluorescence spectral signatures-characterization of phytoplankton populations by the use of excitation and emission-spectra, Journal of Marine Research, Vol. 37, No. 3, pp. 471-483.
Yentsch, C.M., and Pomponi, S.A., (1986), Automated individual call analysis in aquatic research. Int. Rev. Cytol., No. 105, pp. 183-243.
Young, W.F., Horth, H., Crane, R., Ogden, T., and Arnott, M., (1996), Taste and odor threshold concentrations of potential potable water contaminants, Water Research, Vol. 30, pp. 331-340.
Young, C.C., Suffet, I.H., Crozes, G., and Bruchet, A., (1999), Identification of a woody-hay odor-causing compound in a drinking water supply, Water Science & Technology, Vol. 40, No. 6, pp. 273-278.
Zimmermann, R., and Meyer-Reil L.A., (1974), A new method for fluorescence staining of bacterial populations on membrane filters, Kiel. Meeresforsch., No. 30, pp. 24-27.
王奕軒,(2006),自來水中木頭味物質β-cyclocitral之來源及去除之研究,國立成功大學環境工程學系碩士論文。
連紹凱,(2008),前加氯對三種藍綠菌菌體破壞及其代謝物釋出之研究,國立成功大學環境工程學系碩士論文。
曾韻璇,(2008),前氧化對兩種產毒藍綠菌破壞及其代謝物釋出之研究,國立成功大學環境工程學系碩士論文。
李明潔,(2008),臭氧對兩種產臭藍綠菌菌體破壞及其代謝物釋出之研究,國立成功大學環境工程學系碩士論文。
張德威,(2008),淨水程序對藍綠菌細胞破壞及其代謝物去除之研究,國立成功大學環境工程學系碩士論文。
陳振正,(2005),高錳酸鉀與臭氧對混凝除藻之影響,國立成功大學環境工程學系博士論文。
彭惠君,(2002),高錳酸鉀對水中有機物去除機制之研究,國立成功大學環境工程學系碩士論文。
行政院環保署,(2003),台灣地區環境水質監測年報:水庫水質篇。
林財富(2002),水中geosmin與methylisoborneol檢測方法建立,行政院環境保護署。
連結至畢業學校之論文網頁點我開啟連結
註: 此連結為研究生畢業學校所提供,不一定有電子全文可供下載,若連結有誤,請點選上方之〝勘誤回報〞功能,我們會盡快修正,謝謝!
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
無相關期刊