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研究生:陳志遠
研究生(外文):Chih-Yuan Chen
論文名稱:陣列式細胞晶片平台應用於單一細胞膜動作電位之量測
論文名稱(外文):The application of array biochip platform in action potential measurement for single cell
指導教授:羅錦興羅錦興引用關係
指導教授(外文):Ching-Hsing Luo
學位類別:碩士
校院名稱:國立成功大學
系所名稱:奈米科技暨微系統工程研究所
學門:工程學門
學類:材料工程學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:67
中文關鍵詞:膜片箝制離子通道微機電
外文關鍵詞:Ion channePatch-clampMEMS
相關次數:
  • 被引用被引用:1
  • 點閱點閱:198
  • 評分評分:
  • 下載下載:15
  • 收藏至我的研究室書目清單書目收藏:0
細胞膜片箝制技術(patch-clamp technique),是電生理學中研究離子通道機制的工具,傳統膜片箝制方式是使用玻璃吸管(glass pipette),配合AgCl電極接觸在細胞膜上,記錄通過細胞膜的離子電流,瞭解疾病細胞內的機制,此方式可得到正確且精準的資訊,卻需要純熟的操作技術,且相當耗費時間但只能得到少量的紀錄資訊。
本研究利用微機電技術(micro-electro-mechanical systems, MEMS)來製作細胞晶片平台,取代傳統的膜片箝制,量測通過細胞膜的離子電流以及動作電位(action potential),能夠更迅速的得到正確的電位特性,大幅的減少操作時間,提高效率,改進傳統玻璃管微電極的缺點。在本實驗室長久的努力之下,目前已把膜片箝制晶片製造出來,本文中以改變晶片結構的材料、製造程序,並增加晶片封裝、流道設計,改善晶片測試過程中,所產生電容值過大,造成細胞電生理訊號量測困難,因此,本文將針對以上問題來解決,讓晶片達到與玻璃吸管的效果,並節省更多的時間,並且在未來將可對本操作平台進行最佳化的實驗與測試,也可瞭解細胞膜上離子通道與藥物之間的關係,配合上細胞模型的研究,將可達成新藥物開發的目的。
Patch-Clamp technique, an invention in electrophysiology is to make a study of ion channels. As with the conventional cell-attached patch clamp recording method, a glass pipette is used to make tight contact with a cell membrane in order to measure the ion electrical current flowing through the cell membrane and observe the illness cells. In this conventional way, correct and accurate information can be acquired, but sophisticated operation techniques are needed. It wastes time and can only get a little of information.

In this study, MEMS technology is used to produce the platform of cell-chip. It replaces the traditional patch-clamp technique to measure the flow of the ion electrical current and the action potential of cells. In this way, accurate characters of the potential can be obtained faster, and shortcomings in Patch-Clamp technique can be prevented.

After a long-term effort in the laboratory, the current patch-clamp chips have come out in this article to change the structure of the chip materials, manufacture processes, increase the chip package, and design channel. To improve the process by testing chip, the resulting of capacitance value is too large, so that it causes the difficult problems of measuring the cell electrophysiological signals. Therefore, this article will focus on the solutions above these problems to let chips to achieve the effect upon pipette. It saves more time and it will be carried out on the best operating platform of the experiment and test in the future, but also understands the relationship between ion channels and drugs on the cell membrane. With the research on the cell model, it will be able to reach a new drug development purpose.
目錄
摘要(中文) I
摘要(英文) III
致謝 IV
目錄 V
圖目錄 VI
表目錄 X

第一章 序論 1
1.1 前言 1
1.2 微機電製程技術簡介 1
1.3 文獻回顧 3
1.4 研究動機 9
1.5 論文架構 9

第二章 研究背景 11
2.1 離子通道 11
2.2 膜片箝制技術 12
2.2.1 前言 12
2.2.2 膜片箝制原理 12
2.2.3 膜片箝制模式 13
2.2.4 全細胞式膜片等效電路 14
2.3 微機電製程技術介紹 16
2.3.1 RCA clean 16
2.3.2 化學氣相沉積 16
2.3.3 微影製程 17
2.3.4 蝕刻製程 20

第三章 晶片設計與封裝 22
3.1 晶片製作流程與其改進策略 22
3.2 晶片製程與討論 30
3.3 晶片封裝 37

第四章 電容效應 39
4.1 膜片箝制量測結果 39
4.2 電容效應 40
4.2.1 晶片等效電路推導 40
4.2.2 PSpice電路模擬 50

第五章 實驗結果 51
5.1 晶片完成圖 51
5.2 電容效應量測結果 56
5.3 PSpice模擬結果 59
5.4 流道封裝 61

第六章 總結與未來研究方向 63
6.1 討論與總結 63
6.2 未來研究方向 64

References 66
References

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