臺灣博碩士論文加值系統

大鼠經照射不同紫外線劑量後，由尾巴抽取血液，一部份做生理條件量測，一部份分離出紅血球細胞，使用原子力顯微鏡（AFM）量測紅血球細胞獲得力量-位移曲線，再計算出紅血球細胞的彈性模數，分析探討紅血球細胞膜彈性模數對紫外線劑量相關性，以及大鼠經照射不同紫外線劑量後其血液生理條件之變化，進一步並觀察表皮皮膚的變化。由實驗分析結果得到；未照射紫外線的紅血球細胞，其上、下針彈性模數分別為261.59 ± 36.49 kPa及252.94 ± 39.74 kPa，經過t檢定後，兩者沒有顯著性差異；經過紫外線照射後，初期的細胞彈性模數會有明顯的下降趨勢，隨著紫外線累積劑量的增加，下降趨勢逐漸減緩並慢慢上升趨向一穩定值，在長波紫外線（UVA）部份，累積劑量達到432 J/cm2時，與正常細胞相比較下，彈性模數降低約40-60 kPa；在中波紫外線（UVB）部份，累積劑量達到43.2 J/cm2時，彈性模數降低約60-70 kPa；血液生理條件分析方面，白血球（WBC）的變化百分率為最劇烈，而平均血球容積（MCV）、平均血球血紅素（MCH）及平均血球血紅素濃度（MCHC）變化百分率則不明顯；照射UVB累積劑量達32.4 J/cm2後會使老鼠表皮皮膚明顯受傷，並使血液中WBC上升；照射UVA後老鼠表皮皮膚的顏色會隨著累積劑量的增加而漸漸變粉紅色，而照射UVB後除了會造成老鼠表皮皮膚受傷外，其表皮皮膚的顏色隨著照射劑量的增加呈現深紅色。

The blood taken from the rat tail was exposed to different doses of radiation ultraviolet. One part of blood was taken to detect physical conditions and the other to isolate of the force-displacement curve red blood cells that were measured by atomic force microscope (AFM), and the young’s modulus of red blood cells were calculated. The correlation among the young’s modulus of red blood cells, the changes in physiological conditions of blood affected with ultraviolet doses were investigated after the rat exposured to different doses of ultraviolet; furthermore, the changes in the epidermis skin of rats were observed. The experimental results and analysis showed that the young’s modulus of normal red blood cells are 261.5 9± 36.49 kPa and 252.94 ± 39.74 kPa for probe up and down measurement to AFM, respectively. The young’s modulus of red blood cells for probe up and down measurement to AFM was checked with T test, there were no significant difference each other. After red blood cells exposed to received ultraviolet irradiation, the initial young’s modulus of cells changed correspondent to down with ultraviolet dose, the value trend to stable in more dose. When the rats were cumulatived dose 432 J/cm2 exposed to long-wave ultraviolet (UVA), the young’s modulus of cells were compare to reduced 40-60 kPa for normal cells. When the rats were cumulatived dose 43.2 J/cm2 exposed to medium wave ultraviolet (UVB), the young’s modulus of cells reduced 60-70 kPa. Form the analysis of physiological conditions in blood, the of change white blood cells (WBC) has most dramatic change(%), however the changes(%) of mean corpuscular volume（MCV）, mean corpuscular hemoglobin（MCH）, and mean corpuscular hemoglobin concentration（MCHC）were relatively small. When the cumulatived dose got 32.4 J/cm2 by UVB, the epidermal skin of the rat were showed apparent injury and the white blood cells increase. After UVA dose increased, the color of epidermal skin gradually changes to pink. In addition, the increase of UVB dose not only injured he epidermal skin of rat but also changed the skin color to deep red.

目錄
誌　謝 I
摘　要 II
Abstract III
目錄 V
表目錄 VII
圖目錄 VIII
附錄目錄 X
符號說明 XII
第一章 前言 1
1-1　研究背景 1
1-2　研究目的 4
第二章 文獻探討 5
2-1　AFM起源與應用 5
2-2　AFM對細胞的研究 6
2-3　紫外線對細胞的影響 13
第三章 理論基礎 15
3-1　AFM原理 15
3-1-1　AFM量測 15
3-1-2　系統架構 18
3-1-3　AFM探針 20
3-1-4　力量-位移曲線量測原理 21
3-1-5　生物樣本彈性模數分析 23
3-2　紅血球 25
3-3　紫外線 27
第四章 實驗材料與方法 29
4-1　實驗動物 29
4-2　紫外線照射系統 30
4-3　樣品製備使用設備 32
4-4　原子力顯微鏡AFM 36
4-4-1　AFM探針規格 41
4-5　實驗方法 42
4-5-1　血液生理條件分析 43
4-5-2　血液分析計算 44
4-5-3　紅血球細胞採取 45
4-5-4　製作紅血球檢測玻片 46
4-5-5　照射不同紫外線劑量的紅血球細胞 46
第五章 結果與討論 49
5-1　紅血球細胞膜力量-位移曲線與彈性模數 49
5-2　紫外線照射大鼠後以AFM檢測紅血球彈性模數 52
5-2-1　UVA　長波紫外線 54
5-2-2　UVB　中波紫外線 56
5-3　紫外線照射大鼠後分析血液生理條件 58
5-3-1　UVA　長波紫外線 59
5-3-2　UVB　中波紫外線 63
5-4　紫外線照射後表皮皮膚的變化 67
5-4-1　UVA　長波紫外線 67
5-4-2　UVB　中波紫外線 70
第六章 結論與建議 72
6-1　結論 72
6-2　建議 73
參考文獻 74

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