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研究生:陳豐文
研究生(外文):Feng-Wuen Chen
論文名稱:芝麻油體鈣蛋白在微胞系統內結構與穩定度之分析
論文名稱(外文):The stability and structure analysis of sesame caleosin in micelle system
指導教授:錢嘉琳
指導教授(外文):Chia-Lin Chyan
學位類別:碩士
校院名稱:國立東華大學
系所名稱:化學系
學門:自然科學學門
學類:化學學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:69
中文關鍵詞:DMPC/DHPC 微胞SDS微胞微胞系統動態光散射儀圓二色性光譜儀油體鈣蛋白
外文關鍵詞:DLSmicelle systemSDS micelleDMPC/DHPC micellecaleosinCD
相關次數:
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  • 收藏至我的研究室書目清單書目收藏:1
油體鈣蛋白(caleosin)是芝麻油體構造中,除了油體膜蛋白(oleosin)和油體固醇蛋白(steroleosin)之外,另一重要的結構蛋白,其對油體胞器的穩定具有一定的影響。因此在本研究中,我們設計不同的微胞系統:dimyristoylphosphatidylcholine(DMPC) /dihexanoylphosphatidychlone(DHPC)和Sodium dodecyl sulfate(SDS)微胞,以此當作模型模擬芝麻油體的環境,在常溫下觀察油體鈣蛋白C端、N端和IC端三個部分的結構,及其結構對微胞粒徑穩定度的影響。
本研究中,我們使用Circular Dichorism (CD) 和Dynamic Light Scattering (DLS)這兩種實驗儀器來同步觀察油體鈣蛋白在微胞系統中的結構變化與微胞粒徑的穩定度。我們藉由CD光譜儀來分析在不同微胞系統中,油體鈣蛋白的二級結構,DLS則是觀測溶液中分子粒徑大小常用的分析儀器,在此我們用來偵測蛋白質和微胞作用所形成的微胞粒徑分析及其隨時間粒徑之變化。最後則用顯微鏡來觀察不同的油體鈣蛋白部位與phospholipid(PL)及triacylglycerol(TAG)所組成的油體的構造,並和微胞系統的研究結果作對照。
實驗結果顯示,油體鈣蛋白C 端在兩種微胞系統:DMPC/DHPC
和SDS微胞均有結構產生,且結構隨時間改變都能固定不變,所形成的微胞粒徑也很穩定,IC端在兩種微胞系統則只有在1:300的SDS微胞系統有穩定的微胞粒徑存在,油體觀察結果中,發現IC端、C端構成的油珠在顯微鏡下的大小與分佈,和其在SDS(IC端為1:300,C端為1:1000)微胞中分子粒徑分佈程度相似,因此,未來我們可利用SDS微胞系統來對油體鈣蛋白結構作進一步研究。
中文摘要 i
目錄 iii
表目錄 v
圖目錄 vii
一、文獻探討 1
二、材料與方法 9
三、結果與討論 19
四、結論 29
五、參考文獻 31
六、附錄 61
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