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研究生:黎守智
研究生(外文):Li, Shou-chih
論文名稱:馬拉松競賽對氧化壓力與抗氧化之反應
論文名稱(外文):Response of Oxidative Stress and Antioxidant after a Marathon Race
指導教授:謝錦城謝錦城引用關係
指導教授(外文):Hsieh, Chin-Chen
學位類別:碩士
校院名稱:國立新竹教育大學
系所名稱:體育學系碩士班
學門:民生學門
學類:運動休閒及休閒管理學類
論文種類:學術論文
論文出版年:2008
畢業學年度:97
語文別:中文
論文頁數:75
中文關鍵詞:抗氧化系統榖胱甘肽還原態榖胱甘肽氧化態脂質過氧化榖胱甘肽的氧化還原比值
外文關鍵詞:anti-oxidant systemglutathione(GSH)oxidized glutathione(GSSG)lipid peroxidationglutathione/oxidized glutathione(GSH/GSSG ratio)
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本研究在於探討馬拉松競賽後,選手體內自由基及氧化壓力的情形。徵召12位男性選手參加台北國際馬拉松競賽,完成全程42.195公里,平均年齡39.33 ± 9.40歲、身高169.04 ± 6.48公分、體重65.63 ± 6.05公斤、比賽成績212.83 ± 35.4分。在賽前二天 (D0) 、賽後一小時 (1 hr) 、兩小時 (2 hr) 、二十四小時 (24 hr) 以及四十八小時 (48 hr) 採集血液樣本,分別測出各時間序列點血液中過氧化氫 (hydrogen peroxide, H2O2) 、榖胱甘肽還原態 (glutathione, GSH) 、穀胱甘肽的氧化態 (oxidized glutathione, GSSG) 及丙二醛 (malondialdehyde, MDA) ,並進一步計算出GSH/GSSG比值。所得數據資料,以相依樣本單因子變異數分析 (One-way ANOVA with repeated measures) 進行考驗,達顯著差異再以杜凱氏法 (Tukey’s method) 將各時間點與賽前進行事後比較。
所得結果如下。
一、賽後1小時過氧化氫濃度顯著增加 (p < .05) ,於第2小時逐漸恢復。
二、賽後1小時丙二醛濃度顯著增加 (p < .05) ,於第2小時逐漸恢復。
三、榖胱甘肽還原態在賽後1小時、2小時濃度顯著下降 (p < .05) ,於賽後24小時恢復。
四、榖胱甘肽氧化態在賽後1小時、2小時、24小時濃度顯著增加 (p < .05) ,於賽後48小時恢復。
五、GSH/GSSG比值在賽後1小時、2小時、24小時顯著下降 (p < .05) ,於賽後48小時恢復。
本研究的馬拉松運動,誘發體內過氧化氫大量產生,形成氧化壓力,使得賽後有脂質過氧化反應而產生氧化傷害,同時穀胱甘肽系統啟動去平衡體內的過氧化氫,於第2小時丙二醛恢復原來水準,而抗氧化系統至48小時後恢復。顯示長時間高強度的馬拉松運動,抗氧化系統可以解除體內產生的氧化壓力。
The Purpose of this study was to investigate the mutual condition of oxidation stress and free radical affect a runner after a marathon race. There were 12 male contestants to compete in the International Taipei Marathon race, and completed a total distance of 42.195 km, the average age of 39.33 ± 9.40 yrs, height of 169.04 ± 6.48 cm, and weight of 65.63 ± 6.05 kg, average running time of 212.83 ± 35.4 min. Blood sample were collected two days before the race (D0) , one hour (1 hr) , two hours (2 hr) , twenty four hours (24 hr) , and forty eight hours (48 hrs) after the marathon race. The five different time course consistency of hydrogen peroxide (H2O2), glutathione (GSH), oxidized glutathione (GSSG) and malondialdehyde (MDA) were measured. And further counted GSH/GSSG ratio. The data were assessed by one-way analysis of variance (one-way ANOVA with repeated measures) and Tukey’s method to post compare.
The results of the study were shown as follows:
1. The H2O2 concentration was significantly increase (p < 0.5) in the 1 hour after the race, but recovered after 2 hours.
2. The MDA concentration was significantly increase (p < 0.5) in the 1 hour after the race, but recovered after 2 hours.
3. The GSH concentration was decreased gradually after the race, while it was significantly decreased (p < 0.5) after 1 hour and 2 hours of the race, but recovered after 24 hours.
4. The GSSG concentration was significantly increase (p < 0.5) after 1 hour, 2 hours and 24 hours, but recovered after 48 hours.
5. The GSH/GSSG ratio showed a significant decrease (p < 0.5) after 1 hour, 2 hours and 24 hours, but recovered after 48 hours.
The result shows that H2O2 is largely produced after a marathon race and brings oxidative stress. It produces lipid peroxidation and leads to oxidative damage, and this is when GSH system starts to balance the H2O2 within the body. MDA recovers the baseline after 2 hours, and antioxidant system recovers after 48 hours. It is concluded that antioxidant system removes from oxidative damage in body after long and exhaustive marathon race.
Keywords: anti-oxidant system, glutathione(GSH), oxidized glutathione(GSSG), lipid peroxidation, glutathione/oxidized glutathione(GSH/GSSG ratio)
中文摘要 Ⅰ
英文摘要 Ⅱ
目 次 Ⅳ
表 次 Ⅴ
圖 次 Ⅵ
第壹章 緒 論 1
第一節 研究背景 1
第二節 研究目的 4
第三節 研究假設 5
第四節 研究範圍與限制 6
第五節 名詞操作性定義 7
第貳章 文獻探討 8
第一節 自由基的來源 8
第二節 脂質過氧化反應 12
第三節 抗氧化系統 15
第四節 長時間高強度運動下造成的脂質過氧化與氧化壓力 19
第五節 穀胱甘肽系統 23
第參章 研究方法 26
第一節 受試者 26
第二節 實驗設計與流程 27
第三節 樣本分析方法與使用儀器 29
第四節 資料處理 34
第肆章 結 果 35
第一節 受試者基本資料 35
第二節 馬拉松運動後血漿脫水的影響 36
第三節 馬拉松運動後對丙二醛濃度的影響 37
第四節 馬拉松運動後對體內過氧化氫濃度的影響 39
第五節 馬拉松運動後對還原態穀胱甘肽濃度的影響 41
第六節 馬拉松運動後對氧化態穀胱甘肽濃度的影響 43
第七節 馬拉松運動後GSH/GSSG比值變化 45
第伍章 討論與結論 46
第一節 脫水校正之必要性 46
第二節 馬拉松運動後過氧化氫時間序列的影響 48
第三節 馬拉松運動後丙二醛時間序列的影響 50
第四節 馬拉松運動後對穀胱甘肽系統的影響 52
第五節 結論與建議 55

參考文獻 56
中文部份 56
西文部份 58
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