跳到主要內容

臺灣博碩士論文加值系統

(34.204.198.73) 您好!臺灣時間:2024/07/21 15:39
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:李欣珮
研究生(外文):Hsin-Pei Li
論文名稱:原核系統表現家禽介白素-18重組蛋白及其免疫佐劑之活性評估
論文名稱(外文):Adjuvanicity of Prokaryotic Expressing Avian IL-18
指導教授:莊秀琪
指導教授(外文):Hso-Chi Chaung
學位類別:碩士
校院名稱:國立屏東科技大學
系所名稱:生物科技研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
畢業學年度:97
語文別:中文
論文頁數:68
中文關鍵詞:介白素-18干擾素-γ佐劑
外文關鍵詞:chickeninterleukin-18IFN-γadjuvant
相關次數:
  • 被引用被引用:0
  • 點閱點閱:182
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
Interleukin-18 (IL-18) 可誘導Th1細胞產生大量的IFN-γ,研究證實
chicken interleukin-18 (chIL-18) 具有潛力可作為一種新穎安全的免疫佐
劑,本研究構築含全長及成熟之家禽IL-18序列,其表現載體編碼蛋白分
別為198及169胺基酸組成,經純化之重組蛋白以硫酸十二酯鈉-聚丙烯醯
胺膠體電泳 (sodium dodecyl sulfate-polyacrylamide gel electrophoresis,
SDS-PAGE) 及西方轉漬法分析 (Western blotting assay) 確認其分子量,
分別為23.65及21.78 KDa,再以雞脾臟細胞與巨噬細胞評估其免疫活性,
結果顯示chIL-18重組蛋白具有誘導免疫細胞產生IFN-γ及巨噬細胞產生
NO的能力。配合雞新城病 (Newcastle disease;ND) 之不活化疫苗免疫
無特定病原性雞隻以評估其佐劑效力,於生體內有促進ND不活化疫苗之
抗體力價上升及週邊血液單核球細胞增殖作用等保護效力之作用,因此
chIL-18重組蛋白可作為佐劑應用於雞隻疫苗之開發。
Interleukin-18 (IL-18) can stimulate Th1 cells to produce a large amount
of IFN-γ. It has been confirmed that chicken interleukin-18 (chIL-18) has
potential as a novel and safe alternative adjuvant in chicken vaccine. The
objectives of this study are to express recombinant protein of chIL-18-Full
and chIL-18-Mature using a prokaryotic system, and to evaluate the
bioactivities and application of chIL-18. The complete cDNAs of
chIL-18-Full and chIL-18-Mature encode proteins consisting of 198 amino
acids and 169 amino acids, respectively. The recombinant chIL-18-Full and
chIL-18-Mature were purified and analysed using SDS-PAGE and Western
blotting. The molecular weights of chIL-18-Full and chIL-18-Mature
recombinant proteins are 23.65 KDa and 21.78 KDa, respectively. The in vitro
bioactivity of recombinant chIL-18 was evaluated using chicken splenocytes
and macrophages. The adjuvant effect of chIL-18 was evaluated by
coadministrating with inactivated NDV (Newcastle disease) vaccine into the
SPF chicken. The results showed that both chIL-18-Full and chIL-18-Mature
effectively increased IFN-γ gene expression in splenocyte, and thesupernatant of the cultured splenocyte enhanced the NO production in
macrophages. Moreover, chIL-18 can increase HI titer and peripheral blood
mononuclear cell proliferation, and thus it exerts effectively protective immune
functions when coadministrated with inactivated NDV vaccine. Therefore,
chIL-18 protein can be used as a adjuvant for chicken vaccines.
中文摘要............................................................................................................I
Abstract ............................................................................................................ II
謝誌.................................................................................................................IV
目錄..................................................................................................................V
圖表目錄....................................................................................................... VII
第1章 前言....................................................................................................... 1
第2章 文獻探討............................................................................................... 3
2.1 疫苗佐劑之種類與作用....................................................................... 3
2.2 細胞激素之免疫佐劑活性................................................................... 5
2.3 介白素-18 ............................................................................................. 6
2.3.1 介白素-18之細胞來源與生物活性................................................ 7
2.3.2 家禽介白素-18.............................................................................. 12
2.4 介白素-18於家禽疫苗之應用........................................................... 13
第3章 材料與方法......................................................................................... 14
3.1 試驗材料............................................................................................. 14
3.1.1 家禽介白素-18之選殖.................................................................. 14
3.1.2 以原核表現系統表現重組家禽介白素-18蛋白.......................... 17
3.1.3 重組家禽介白素-18蛋白之純化與確認...................................... 18
3.1.3.1 重組蛋白之純化..................................................................... 18
3.1.3.2 SDS-PAGE確認重組蛋白...................................................... 19
3.1.3.3 Western blotting確認重組蛋白.............................................. 20
3.1.4 重組家禽介白素-18蛋白之免疫活性評估.................................. 21
3.1.4.1 家禽介白素-18於細胞IFN-γ及NO之影響............................ 21
3.1.4.2 重組家禽介白素-18蛋白應用於家禽疫苗之免疫活性評估21
3.2 試驗方法............................................................................................. 22
3.2.1 家禽介白素-18之選殖.................................................................. 22
3.2.2 以原核表現系統表現重組家禽介白素-18蛋白.......................... 27
3.2.3 重組家禽介白素-18蛋白之純化與確認...................................... 29
3.2.3.1 重組蛋白之純化..................................................................... 29
VI
3.2.3.2 SDS-PAGE確認重組蛋白...................................................... 30
3.2.3.3 Western blotting確認重組蛋白.............................................. 30
3.2.4 重組家禽介白素-18蛋白之免疫活性評估.................................. 31
3.2.4.1 家禽介白素-18於細胞IFN-γ及NO之影響............................ 31
3.2.4.2 重組家禽介白素-18蛋白應用於家禽疫苗之免疫活性評估33
3.2.4.3 統計分析................................................................................. 34
第4章 結果..................................................................................................... 35
4.1 家禽介白素-18之選殖....................................................................... 35
4.2 以原核表現系統表現重組家禽介白素-18蛋白............................... 35
4.3 重組家禽介白素-18蛋白之免疫活性評估....................................... 35
4.3.1 家禽介白素-18 於細胞IFN-γ及NO之影響................................ 35
4.3.2 重組家禽介白素-18蛋白應用於家禽疫苗之免疫活性評估..... 36
第5章 討論..................................................................................................... 38
圖表… ............................................................................................................. 41
參考文獻......................................................................................................... 50
附錄................................................................................................................. 62
作者簡介......................................................................................................... 68
Ahmed, K.A., Saxena, V.K., Ara, A., Singh, K.B., Sundaresan, N.R., Saxena, M., and Rasool, T.J. (2007) Immune response to Newcastle disease virus in chicken lines divergently selected for cutaneous hypersensitivity. Int J Immunogenet 34:445-455.

Ait-Oufella, H., Taleb, S., Mallat, Z., and Tedgui, A. (2009) Cytokine network and T cell immunity in atherosclerosis. Semin Immunopathol.

Asif, M., Jenkins, K.A., Hilton, L.S., Kimpton, W.G., Bean, A.G., and Lowenthal, J.W. (2004) Cytokines as adjuvants for avian vaccines. Immunol Cell Biol 82:638-643.

Barr, T.A., Carlring, J., and Heath, A.W. (2006) Co-stimulatory agonists as immunological adjuvants. Vaccine 24:3399-3407.

Bhagat, J., Ahmed, K.A., Tyagi, P., Saxena, M., and Saxena, V.K. (2008) Effects of supplemental chromium on interferon-gamma (IFN-gamma) mRNA expression in response to Newcastle disease vaccine in broiler chicken. Res Vet Sci 85:46-51.

Biet, F., Locht, C., and Kremer, L. (2002) Immunoregulatory functions of interleukin 18 and its role in defense against bacterial pathogens. J Mol Med 80:147-162.

Born, T.L., Thomassen, E., Bird, T.A., and Sims, J.E. (1998) Cloning of a novel receptor subunit, AcPL, required for interleukin-18 signaling. J Biol Chem 273:29445-29450.

Chaudhury, P., and Bera, B.C. (2005) Cloning and sequencing of Indian water buffalo interleukin-18 cDNA. Int J Immunogenet 32:75-78.

Chaudhry, U.I., Kingham, T.P., Plitas, G., Katz, S.C., Raab, J.R., and DeMatteo, R.P. (2006) Combined stimulation with interleukin-18 and CpG induces murine natural killer dendritic cells to produce IFN-gamma and inhibit tumor growth. Cancer Res 66:10497-10504.

Chen, H., Huang, Q., Cui, B., Li, X., and Guan, Q. (2008a) [Construction of recombinant fowlpox virus coexpressing HA from subtype H5 of avain influenza virus and chicken interleukin-18]. Wei Sheng Wu Xue Bao 48:1025-1030.

Chen, H., Li, X., Cui, B., Xia, P., Zhang, H., and Yang, M. (2008b) [Construction of eukaryotic express vector of duck interleukin 18 gene and identification of bioactivity of its expressed protein]. Sheng Wu Gong Cheng Xue Bao 24:1568-1572.

Chen, H.Y., Cui, B.A., Xia, P.A., Li, X.S., Hu, G.Z., Yang, M.F., Zhang, H.Y., Wang, X.B., Cao, S.F., Zhang, L.X., Kang, X.T., and Tu, K. (2008c) Cloning, in vitro expression and bioactivity of duck interleukin-18. Vet Immunol Immunopathol 123:205-214.

Conti, B., Jahng, J.W., Tinti, C., Son, J.H., and Joh, T.H. (1997) Induction of interferon-gamma inducing factor in the adrenal cortex. J Biol Chem 272:2035-2037.

Cox, J.C., and Coulter, A.R. (1997) Adjuvants--a classification and review of their modes of action. Vaccine 15:248-256.

Degen, W.G., van Zuilekom, H.I., Scholtes, N.C., van Daal, N., and Schijns, V.E. (2005) Potentiation of humoral immune responses to vaccine antigens by recombinant chicken IL-18 (rChIL-18). Vaccine 23:4212-4218.

Domeika, K., Berg, M., Eloranta, M.L., and Alm, G.V. (2002) Porcine interleukin-12 fusion protein and interleukin-18 in combination induce interferon-gamma production in porcine natural killer and T cells. Vet Immunol Immunopathol 86:11-21.

Dunham, S.P., Flynn, J.N., Rigby, M.A., Macdonald, J., Bruce, J., Cannon, C., Golder, M.C., Hanlon, L., Harbour, D.A., Mackay, N.A., Spibey, N., Jarrett, O., and Neil, J.C. (2002) Protection against feline immunodeficiency virus using replication defective proviral DNA vaccines with feline interleukin-12 and -18. Vaccine 20:1483-1496.

Elenkov, I.J., Iezzoni, D.G., Daly, A., Harris, A.G., and Chrousos, G.P. (2005) Cytokine dysregulation, inflammation and well-being. Neuroimmunomodulation 12:255-269.

Foss, D.L., Zilliox, M.J., and Murtaugh, M.P. (2001) Bacterially induced activation of interleukin-18 in porcine intestinal mucosa. Vet Immunol Immunopathol 78:263-277.

Fournout, S., Dozois, C.M., Yerle, M., Pinton, P., Fairbrother, J.M., Oswald, E., and Oswald, I.P. (2000) Cloning, chromosomal location, and tissue expression of the gene for pig interleukin-18. Immunogenetics 51:358-365.

Gobel, T.W., Schneider, K., Schaerer, B., Mejri, I., Puehler, F., Weigend, S., Staeheli, P., and Kaspers, B. (2003) IL-18 stimulates the proliferation and IFN-gamma release of CD4+ T cells in the chicken: conservation of a Th1-like system in a nonmammalian species. J Immunol 171:1809-1815.

Gu, Y., Kuida, K., Tsutsui, H., Ku, G., Hsiao, K., Fleming, M.A., Hayashi, N., Higashino, K., Okamura, H., Nakanishi, K., Kurimoto, M., Tanimoto, T., Flavell, R.A., Sato, V., Harding, M.W., Livingston, D.J., and Su, M.S. (1997) Activation of interferon-gamma inducing factor mediated by interleukin-1beta converting enzyme. Science 275:206-209.

Gupta, R.K., Relyveld, E.H., Lindblad, E.B., Bizzini, B., Ben-Efraim, S., and Gupta, C.K. (1993) Adjuvants--a balance between toxicity and adjuvanticity. Vaccine 11:293-306.

Haghighi, H.R., Abdul-Careem, M.F., Dara, R.A., Chambers, J.R., and Sharif, S. (2008) Cytokine gene expression in chicken cecal tonsils following treatment with probiotics and Salmonella infection. Vet Microbiol 126:225-233.

Hanlon, L., Argyle, D., Bain, D., Nicolson, L., Dunham, S., Golder, M.C., McDonald, M., McGillivray, C., Jarrett, O., Neil, J.C., and Onions, D.E. (2001) Feline leukemia virus DNA vaccine efficacy is enhanced by coadministration with interleukin-12 (IL-12) and IL-18 expression vectors. J Virol 75:8424-8433.

Hilton, L.S., Bean, A.G., and Lowenthal, J.W. (2002) The emerging role of avian cytokines as immunotherapeutics and vaccine adjuvants. Vet Immunol Immunopathol 85:119-128.

Holmes, S., Abrahamson, J.A., Al-Mahdi, N., Abdel-Meguid, S.S., and Ho, Y.S. (2000) Characterization of the in vitro and in vivo activity of monoclonal antibodies to human IL-18. Hybridoma 19:363-367.
Hsieh, M.K., Wu, C.C., and Lin, T.L. (2006) The effect of co-administration of DNA carrying chicken interferon-gamma gene on protection of chickens against infectious bursal disease by DNA-mediated vaccination. Vaccine 24:6955-6965.

Huang, Z., Elankumaran, S., Yunus, A.S., and Samal, S.K. (2004) A recombinant Newcastle disease virus (NDV) expressing VP2 protein of infectious bursal disease virus (IBDV) protects against NDV and IBDV. J Virol 78:10054-10063.

Ishizaka, T., Setoguchi, A., Masuda, K., Ohno, K., and Tsujimoto, H. (2001) Molecular cloning of feline interferon-gamma-inducing factor (interleukin-18) and its expression in various tissues. Vet Immunol Immunopathol 79:209-218.

Iwasaka, H., and Noguchi, T. (2004) [Th1/Th2 balance in systemic inflammatory response syndrome (SIRS)]. Nippon Rinsho 62:2237-2243.

Kaiser, P. (2002) Turkey and chicken interleukin-18 (IL18) share high sequence identity, but have different polyadenylation sites in their 3' UTR. Dev Comp Immunol 26:681-687.

Karaca, K., Sharma, J.M., Winslow, B.J., Junker, D.E., Reddy, S., Cochran, M., and McMillen, J. (1998) Recombinant fowlpox viruses coexpressing chicken type I IFN and Newcastle disease virus HN and F genes: influence of IFN on protective efficacy and humoral responses of chickens following in ovo or post-hatch administration of recombinant viruses. Vaccine 16:1496-1503.

Khatri, M., Palmquist, J.M., Cha, R.M., and Sharma, J.M. (2005) Infection and activation of bursal macrophages by virulent infectious bursal disease virus. Virus Res 113:44-50.

Kogut, M.H., Rothwell, L., and Kaiser, P. (2003) Priming by recombinant chicken interleukin-2 induces selective expression of IL-8 and IL-18 mRNA in chicken heterophils during receptor-mediated phagocytosis of opsonized and nonopsonized Salmonella enterica serovar enteritidis. Mol Immunol 40:603-610.

Lian, H., Jin, N., Li, X., Mi, Z., Zhang, J., Sun, L., Li, X., Zheng, H., and Li, P. (2007) Induction of an effective anti-tumor immune response and tumor regression by combined administration of IL-18 and Apoptin. Cancer Immunol Immunother 56:181-192.

Liu, B., Novick, D., Kim, S.H., and Rubinstein, M. (2000) Production of a biologically active human interleukin 18 requires its prior synthesis as PRO-IL-18. Cytokine 12:1519-1525.

Ma, M., Jin, N., Shen, G., Zhu, G., Liu, H.J., Zheng, M., Lu, H., Huo, X., Jin, M., Yin, G., Ma, H., Li, X., Ji, Y., and Jin, K. (2008) Immune responses of swine inoculated with a recombinant fowlpox virus co-expressing P12A and 3C of FMDV and swine IL-18. Vet Immunol Immunopathol 121:1-7.

Mingxiao, M., Ningyi, J., Juan, L.H., Min, Z., Guoshun, S., Guangze, Z., Huijun, L., Xiaowei, H., Minglan, J., Xu, L., Haili, M., Yue, J., Gefen, Y., and Kuoshi, J. (2007) Immunogenicity of plasmids encoding P12A and 3C of FMDV and swine IL-18. Antiviral Res 76:59-67.

Mingxiao, M., Ningyi, J., Zhenguo, W., Ruilin, W., Dongliang, F., Min, Z., Gefen, Y., Chang, L., Leili, J., Kuoshi, J., and Yingjiu, Z. (2006) Construction and immunogenicity of recombinant fowlpox vaccines coexpressing HA of AIV H5N1 and chicken IL18. Vaccine 24:4304-4311.

Moller, B., Kessler, U., Rehart, S., Kalina, U., Ottmann, O.G., Kaltwasser, J.P., Hoelzer, D., and Kukoc-Zivojnov, N. (2002) Expression of interleukin-18 receptor in fibroblast-like synoviocytes. Arthritis Res 4:139-144.

Moller, B., Paulukat, J., Nold, M., Behrens, M., Kukoc-Zivojnov, N., Kaltwasser, J.P., Pfeilschifter, J., and Muhl, H. (2003) Interferon-gamma induces expression of interleukin-18 binding protein in fibroblast-like synoviocytes. Rheumatology (Oxford) 42:442-445.

Morar, D., Tijhaar, E., Negrea, A., Hendriks, J., van Haarlem, D., Godfroid, J., Michel, A.L., and Rutten, V.P. (2007) Cloning, sequencing and expression of white rhinoceros (Ceratotherium simum) interferon-gamma (IFN-gamma) and the production of rhinoceros IFN-gamma specific antibodies. Vet Immunol Immunopathol 115:146-154.

Muhl, H., and Pfeilschifter, J. (2004) Interleukin-18 bioactivity: a novel target for immunopharmacological anti-inflammatory intervention. Eur J Pharmacol 500:63-71.

Muneta, Y., Mori, Y., Shimoji, Y., and Yokomizo, Y. (2000) Porcine interleukin 18: cloning, characterization of the cDNA and expression with the baculovirus system. Cytokine 12:566-572.

Nagata, T., Ishikawa, S., Shimokawa, E., Kamikawa, M., Kikuma, R., Muneta, Y., Yokomizo, Y., Nakamura, M., and Takehara, K. (2002) High level expression and purification of bioactive bovine interleukin-18 using a baculovirus system. Vet Immunol Immunopathol 87:65-72.

O'Donovan, L.H., McMonagle, E.L., Taylor, S., Argyle, D.J., and Nicolson, L. (2004) Bioactivity and secretion of interleukin-18 (IL-18) generated by equine and feline IL-18 expression constructs. Vet Immunol Immunopathol 102:421-428.

O'Donovan, L.H., McMonagle, E.L., Taylor, S., Bain, D., Pacitti, A.M., Golder, M.C., McDonald, M., Hanlon, L., Onions, D.E., Argyle, D.J., Jarrett, O., and Nicolson, L. (2005) A vector expressing feline mature IL-18 fused to IL-1beta antagonist protein signal sequence is an effective adjuvant to a DNA vaccine for feline leukaemia virus. Vaccine 23:3814-3823.

Okamura, H., Tsutsi, H., Komatsu, T., Yutsudo, M., Hakura, A., Tanimoto, T., Torigoe, K., Okura, T., Nukada, Y., Hattori, K., and et al. (1995) Cloning of a new cytokine that induces IFN-gamma production by T cells. Nature 378:88-91.

Okano, F., Satoh, M., Ido, T., and Yamada, K. (1999) Cloning of cDNA for canine interleukin-18 and canine interleukin-1beta converting enzyme and expression of canine interleukin-18. J Interferon Cytokine Res 19:27-32.

Okano, F., and Yamada, K. (2000) Canine interleukin-18 induces apoptosis and enhances Fas ligand mRNA expression in a canine carcinoma cell line. Anticancer Res 20:3411-3415.

Puehler, F., Gobel, T., Breyer, U., Ohnemus, A., Staeheli, P., and Kaspers, B. (2003) A sensitive bioassay for chicken interleukin-18 based on the inducible release of preformed interferon-gamma. J Immunol Methods 274:229-232.

Rajput, Z.I., Hu, S.H., Xiao, C.W., and Arijo, A.G. (2007) Adjuvant effects of saponins on animal immune responses. J Zhejiang Univ Sci B 8:153-161.

Rietschel, E.T., Kirikae, T., Schade, F.U., Mamat, U., Schmidt, G., Loppnow, H., Ulmer, A.J., Zahringer, U., Seydel, U., Di Padova, F., and et al. (1994) Bacterial endotoxin: molecular relationships of structure to activity and function. Faseb J 8:217-225.

Roh, H.J., Sung, H.W., and Kwon, H.M. (2006) Effects of DDA, CpG-ODN, and plasmid-encoded chicken IFN-gamma on protective immunity by a DNA vaccine against IBDV in chickens. J Vet Sci 7:361-368.

Sadeyen, J.R., Trotereau, J., Velge, P., Marly, J., Beaumont, C., Barrow, P.A., Bumstead, N., and Lalmanach, A.C. (2004) Salmonella carrier state in chicken: comparison of expression of immune response genes between susceptible and resistant animals. Microbes Infect 6:1278-1286.

Sanchez-Munoz, F., Dominguez-Lopez, A., and Yamamoto-Furusho, J.K. (2008) Role of cytokines in inflammatory bowel disease. World J Gastroenterol 14:4280-4288.

Schneider, K., Puehler, F., Baeuerle, D., Elvers, S., Staeheli, P., Kaspers, B., and Weining, K.C. (2000) cDNA cloning of biologically active chicken interleukin-18. J Interferon Cytokine Res 20:879-883.

Shao, X.T., Feng, L., Gu, L.J., Wu, L.J., Feng, T.T., Yang, Y.M., Wu, N.P., and Yao, H.P. (2009) Expression of interleukin-18, IL-18BP, and IL-18R in serum, synovial fluid, and synovial tissue in patients with rheumatoid arthritis. Clin Exp Med.

Shen, G., Jin, N., Ma, M., Jin, K., Zheng, M., Zhuang, T., Lu, H., Zhu, G., Jin, H., Jin, M., Huo, X., Qin, X., Yin, R., Li, C., Li, H., Li, Y., Han, Z., Chen, Y., and Jin, M. (2007) Immune responses of pigs inoculated with a recombinant fowlpox virus coexpressing GP5/GP3 of porcine reproductive and respiratory syndrome virus and swine IL-18. Vaccine 25:4193-4202.

Sokolovska, A., Hem, S.L., and HogenEsch, H. (2007) Activation of dendritic cells and induction of CD4(+) T cell differentiation by aluminum-containing adjuvants. Vaccine 25:4575-4585.

Sugiura, K., Akazawa, T., Fujimoto, M., Wijewardana, V., Mito, K., Hatoya, S., Taketani, S., Komori, M., Inoue, N., and Inaba, T. (2008) Construction of an expression vector for improved secretion of canine IL-18. Vet Immunol Immunopathol 126:388-391.

Sundaresan, N.R., Ahmed, K.A., Saxena, V.K., Sastry, K.V., Saxena, M., Pramod, A.B., Nath, M., Singh, K.B., Rasool, T.J., DevRoy, A.K., and Singh, R.V. (2005) Differential expression of inducible nitric oxide synthase and cytokine mRNA in chicken lines divergent for cutaneous hypersensitivity response. Vet Immunol Immunopathol 108:373-385.

Swaggerty, C.L., Kogut, M.H., Ferro, P.J., Rothwell, L., Pevzner, I.Y., and Kaiser, P. (2004) Differential cytokine mRNA expression in heterophils isolated from Salmonella-resistant and -susceptible chickens. Immunology 113:139-148.

Torigoe, K., Ushio, S., Okura, T., Kobayashi, S., Taniai, M., Kunikata, T., Murakami, T., Sanou, O., Kojima, H., Fujii, M., Ohta, T., Ikeda, M., Ikegami, H., and Kurimoto, M. (1997) Purification and characterization of the human interleukin-18 receptor. J Biol Chem 272:25737-25742.

Ushio, S., Namba, M., Okura, T., Hattori, K., Nukada, Y., Akita, K., Tanabe, F., Konishi, K., Micallef, M., Fujii, M., Torigoe, K., Tanimoto, T., Fukuda, S., Ikeda, M., Okamura, H., and Kurimoto, M. (1996) Cloning of the cDNA for human IFN-gamma-inducing factor, expression in Escherichia coli, and studies on the biologic activities of the protein. J Immunol 156:4274-4279.

Vogel, K., Kantor, J., Wood, L., Rivera, R., and Schlom, J. (1998) Oral immunization with enterocoated microbeads induces antigen-specific cytolytic T-cell responses. Cell Immunol 190:61-67.

Wienhold, D., Armengol, E., Marquardt, A., Marquardt, C., Voigt, H., Buttner, M., Saalmuller, A., and Pfaff, E. (2005) Immunomodulatory effect of plasmids co-expressing cytokines in classical swine fever virus subunit gp55/E2-DNA vaccination. Vet Res 36:571-587.

Xu, J., Deng, T.L., Li, L., You, Z.Q., Wan, W.J., and Yu, L. (2005) Nitric oxide inducing function and intracellular movement of chicken interleukin-18 in cultured cells. Acta Biochim Biophys Sin (Shanghai) 37:688-693.

Yang, L., An, X., Wei, F., Liu, H., Li, H., Yu, J., and Ren, X. (2008) Expression and purification of recombinant human interleukin-18 protein using a yeast expression system. Protein Expr Purif 62:44-48.

Ye, X.J., Tang, B., Ma, Z., Kang, A.H., Myers, L.K., and Cremer, M.A. (2004) The roles of interleukin-18 in collagen-induced arthritis in the BB rat. Clin Exp Immunol 136:440-447.

Yin, J., Jin, H., Yang, F., Ding, Z., Huang, C., Zhu, Q., and Wang, B. (2007) Synergistic effects of adjuvants interferon-gamma and levamisole on DNA vaccination against infection with Newcastle disease virus. Viral Immunol 20:288-299.

Yoshimoto, T., and Nakanishi, K. (2006) Roles of IL-18 in basophils and mast cells. Allergol Int 55:105-113.

Yoshimura, K., Hazama, S., Iizuka, N., Yoshino, S., Yamamoto, K., Muraguchi, M., Ohmoto, Y., Noma, T., and Oka, M. (2001) Successful immunogene therapy using colon cancer cells (colon 26) transfected with plasmid vector containing mature interleukin-18 cDNA and the Igkappa leader sequence. Cancer Gene Ther 8:9-16.

Zhu, M., Xu, X., Liu, H., Liu, X., Wang, S., Dong, F., Yang, B., and Song, G. (2003) Enhancement of DNA vaccine potency against herpes simplex virus 1 by co-administration of an interleukin-18 expression plasmid as a genetic adjuvant. J Med Microbiol 52:223-228.
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top