(34.201.11.222) 您好!臺灣時間:2021/02/25 13:33
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果

詳目顯示:::

我願授權國圖
: 
twitterline
研究生:賈正豪
研究生(外文):JHENG-HAO JIA
論文名稱:新式試管型定域電漿共振免標定生物感測器
論文名稱(外文):Novel test-tube-type LPR label-free biosensor
指導教授:李柏旻吳瑋特
指導教授(外文):Pomin LiWei-Te Wu
學位類別:碩士
校院名稱:國立屏東科技大學
系所名稱:生物機電工程系所
學門:工程學門
學類:機械工程學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:88
中文關鍵詞:定域電漿共振鎖相迴路鎖相放大電路試管波導
外文關鍵詞:LPRPLLLIAtest-tube wave guide
相關次數:
  • 被引用被引用:0
  • 點閱點閱:224
  • 評分評分:系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔
  • 下載下載:28
  • 收藏至我的研究室書目清單書目收藏:0
本研究根據貴金屬奈米粒子定域電漿共振(Localized Plasmon Resonance, LPR)感測原理為基礎,使用內管壁修飾奈米金粒子之試管為盛液、量測及波導多功能整合元件,搭配LED光源及PD傳感器設計出新式試管型LPR免標定生物感測器,並且設計鎖相放大電路處理感測訊號,降低雜訊之干擾。且將此試管型LPR生物感測器以不同濃度蔗糖溶液進行濃度梯度測試,測得其感測靈敏度為10-5 RIU。本研究所提出之試管型LPR免標定生物感測器已達成組裝簡單、小型化、低成本及高靈敏度之目標。
This research is based on the theory of localized plasmon resonance of the metal particle in nanometer.This technologie produces a new test tube style LPR biological sensor without binding site, which uses within the wall-modified gold nano-grain for the test-tube as liquid test-tube, measures and optical conduction integrated multi-functional components. Besides, its design can enlarge the induction signal handled by circuit and reduce the interference.We test this new product by a gradient test with different concentration of sucrose solution and its sensitivity is 10-5RIU.
摘 要................................................................................................................I
Abstract.............................................................................................................II
謝 誌..............................................................................................................III
目 錄.............................................................................................................IV
表目錄............................................................................................................VII
圖目錄............................................................................................................VII
第1章 緒論....................................................................................................1
1.1 前言...................................................................................................1
1.2 文獻探討...........................................................................................4
1.2.1 定域表面電漿共振(LPR).....................................................4
1.2.2 鎖相放大器應用於生物感測器.........................................10
1.3 研究動機.........................................................................................14
1.4 研究目的.........................................................................................14
第2章 研究方法..........................................................................................15
2.1 實驗材料與設備.............................................................................15
2.2 研究步驟.........................................................................................16
2.3 訊號處理分析.................................................................................23
2.3.1 鎖相放大器原理.................................................................23
2.2.2 鎖相迴路原理.....................................................................32
2.3.3 RC濾波電路.......................................................................39
2.4 實驗流程.........................................................................................41
2.4.1 溶液的配製.........................................................................41
2.4.2 實驗步驟.............................................................................41
第3章 結果與討論......................................................................................43
3.1 試管型LPR生物感測器訊號量測.................................................43
3.2 LM565鎖相迴路RC濾波電路分析..............................................52
3.3 試管型LPR利用LM565鎖相迴路RC濾波電路處理量測訊 號......................................................................................................56
3.4 試管型LPR利用LM565鎖相迴路RC濾波電路匹配探討...........62
3.5 AD620鎖相放大RC濾波電路分析.............................................. 67
3.6 試管型LPR利用AD630鎖相放大RC濾波電路處理量測訊號71
3.7 試管型LPR利用AD630鎖相放大迴路RC濾波電路匹配探討
........................................................................................................77
第4章 結論與未來展望.......................................82
参考文獻..........................................................................................................84
作者簡介.........................................................................................................89
[1]M.N. Kronick, and W.A. Litlle, 1975,“A new immunoassay based on fluorescence excitation by internal reflection spectroscopy,” J. Immunol. Method, Vol.8, pp.235.
[2]Z. Salamon, H.A. Macleod, and G. Tollin, 1997, “Surface plasmon resonance spectroscopy as a tool for investigating the biochemical and biophysical properties of membrane protein system. II:Applications to biological systems,”Biochimica et Biophysica Acta,Vol.1331,pp.131-152.
[3] S. F. Cheng, L. K. Chau,2003, “Colloidal Gold-Modified Optical Fiber for Chemical and Biochemical Sensing”, Analytical Chemistry, Vol. 75, No. 1,pp.16-21.
[4]Lai-Kwan Chau, Yi-Fang Lin, Shu-Fang Cheng, Tsao-Jen Lin, 2006, “Fiber-optic chemical and biochemical probes based on localized surface plasmon resonance,” Sensors and Actuators B, Vol.113, pp.100-105.
[5]R. A. Potyrailo, S. E. Holbbs, G. M. Hieftje, 1998, “Optical Time-of-Flight Chemical Detection: Absorption-Modulated Fluorescence for Spatially Resolved Analyte Mapping in a Bidirectional Distributed Fiber-Optic Sensor,” Analytical Chemistry,Vol.362,pp,349-373.
[6]Jaw-Luen Tang, Shu-Fang Cheng, Wei-Ting Hsu, Tsung-Yu Chiang, Lai-Kwan Chau 2006,“Fiber-optic biochemical sensing with a colloidal gold-modified long period fiber grating,”Sensors and Actuators B: Chemical, Vol.119, pp.105-109.
[7] Chang K. Choi , Chuck H. Margraves , Seung I. Jun,Anthony E. English ,Philip D. Rack and Kenneth D. Kihm ,2008,“Opto-Electric Cellular Biosensor Using Optically TransparentIndium Tin Oxide (ITO) Electrodes”,Sensors, Vol.8,pp.3257-3270.
[8] David R. Baselt, Gil U Lee, and Richard J. Colton,1996, “Biosensor based on force microscope technology”,J. Vac. Sci. Technol. B, Vol.14, No. 2, pp.789-793.
[9]Richard Luxton, Jasvant Badesha, Janice Kiely, and Peter Hawkins,2004,“Use of External Magnetic Fields To Reduce Reaction Times in an Immunoassay Using Micrometer-Sized Paramagnetic Particles as Labels (Magnetoimmunoassay)”, Analytical Chemistry,Vol.76, pp.1715-1719.
[10]A. B. Lobo Ribeiro, L. A. Fereira, J. L. Santos, and D. A. Jackson, 1997,“ A n a l y s i s o f t h e r e f l e c t i v e-matched fiber Bragg grating sensing interrogation scheme,” Applied Optics, Vol. 36, No. 4,
pp.934-939.
[11]M. J. Gander, W. N. MacPherson, R. McBride, J. D. C. Jones,L. Zhang, I. Bennion, P.M. Blanchard, J. G. Burnett ,and A.H.Greenaway, 2000, “Bend measurement using Bragg gratings in multicorefibre,” Electron. Lett,Vol. 36, No.2, pp.120-121.
[12]S. C. Tjin, J. Z. Hao, and R. Malik, 1998, “Fiber optic Pressure Sensor using Fiber Bragg Grating,” SPIE,Vol. 3429, pp.123-129.
[13]B. Ortega, J. Capmany, Senior Member, IEEE, D. Pastor, Associate Member, IEEE, L. Tallone, and L. Boschis, 1998,“Analysis of the Backreflected Signal in an All-Fiber Banbpass Bragg Transmission Filter,” IEEE Photon. Technol. Lett. Vol. 10, No.8, pp.1124-1126.
[14]S. Jin, R. P. Espindola, H. Mavoori, T. A. Strasser, and J. J. DeMarco, 1998, “Magnetically programmable fibre Bragg gratings,” Electron.Lett. vol. 34, no. 22, pp.2158-2159.
[15]L.Meade, 1983"Lock-in amplifiers : principles and applications,"
[16]K. K. Hung, P. K. Ko, C. Hu, and Y. Cheng, 1990, “A physics-basedMOSFET noise model for circuit simulators”, IEEE Transactions onElectron Devices, Vol.37, pp.1323-1333.
[17]J. Chang, A. A. Abidi, and C. R. Viswanathan, 1994, “Flicker noise in CMOS transistors from subthreshold to strong inversion at various temperatures”, IEEE Transactions on Electron Devices, Vol.41, pp.1965-1971.
[18]C. G. Jakobson, and Y. Nemirovsky, 1999, “1/f Noise in ion sensitive field effect transistors from subthreshold to saturation”, IEEE Transactions on Electron Devices, Vol.46, pp.259-261
[19]M. Aizawa, 1994, “Immunosensor for Clinical Analysis,” Adv. Clin. Chem., Vol.31, pp. 247-275.
[20] SR810 DSP 1993 “Lock-in Amplifier Operating Manual and Programming Reference,” Stanford Research System Inc.
[21]M. A. Gdeisat, D. R. Burton, and M. J. Lalor, 2002, “Non-continuous fringe pattern demodulation using a digital phase locked loop algorithm,” SPIE Proc, Vol.4777, pp.259-270.
[22]S. Kim et al., 1997, “A 960-Mb/s/pin Interface for Skew-Tolerant Bus Using Low Jitter PLL”, IEEE Journal of Solid Sate Circuits, Vol. 32, pp. 691-699,
[23] V. Kaenel et al., 1996, “A 320 MHz, 1.5 mW 1.35 V CMOS PLL for
Microprocessoer Clock generation”, IEEE Journal of Solid Sate Circuits, Vol.31, No. 11, pp. 1715-1722.
[24] I. Novof et al., 1995, “Fully Integrated CMOS Phase-Locked Loop with 15 to 240MHz Locking Range and ±50 ps Jitter”, IEEE Journal of Solid Sate Circuits, Vol. 30, pp. 1259-1266.
[25] J. Maneatic et al. 1996, “Low-Jitter Process-Independent DLL and PLL Based on Self-Biased techniques”, IEEE Journal of Solid State Circuits, Vol. 31, pp. 1723-1732.
[26] G. Chyun Hsieh, Hung J.C, 1996,“Phase-locked loop techniques. A survey”, IEEE Journal of Solid State Circuits, Vol. 43, No 6, pp. 609–615.
[27] O. Henrik, 1998 “A Simple Precharged CMOS Phase Frequency Detector”, IEEE Journal of Solid State Circuits, Vol. 33, pp. 295-299,
[28]Hwang T C (1994) Experiments in Physical Chemistry. Gaulih Book Co., Taipei
[29] James O. Westgard, Ph.D. 2008“Basic Method Validation”,Third Edition.
[30] Armbuster DA, Tillman MD, Hubbs LM. 1994“Limit of detection (LOD)/limit of quantitation (LOQ): Comparison of the empirical and the statistical methods exemplified with GC-MS assays of abused drugs”. Clin Chem;Vol,40,pp.1233-1238.
[31] 許偉庭, 2005 “光纖式LSPR 微流體晶片感測器”,碩士論文, 國立中正大學。
連結至畢業學校之論文網頁點我開啟連結
註: 此連結為研究生畢業學校所提供,不一定有電子全文可供下載,若連結有誤,請點選上方之〝勘誤回報〞功能,我們會盡快修正,謝謝!
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
系統版面圖檔 系統版面圖檔