本研究根據貴金屬奈米粒子定域電漿共振(Localized Plasmon Resonance, LPR)感測原理為基礎，使用內管壁修飾奈米金粒子之試管為盛液、量測及波導多功能整合元件，搭配LED光源及PD傳感器設計出新式試管型LPR免標定生物感測器，並且設計鎖相放大電路處理感測訊號，降低雜訊之干擾。且將此試管型LPR生物感測器以不同濃度蔗糖溶液進行濃度梯度測試，測得其感測靈敏度為10-5 RIU。本研究所提出之試管型LPR免標定生物感測器已達成組裝簡單、小型化、低成本及高靈敏度之目標。

This research is based on the theory of localized plasmon resonance of the metal particle in nanometer.This technologie produces a new test tube style LPR biological sensor without binding site, which uses within the wall-modified gold nano-grain for the test-tube as liquid test-tube, measures and optical conduction integrated multi-functional components. Besides, its design can enlarge the induction signal handled by circuit and reduce the interference.We test this new product by a gradient test with different concentration of sucrose solution and its sensitivity is 10-5RIU.

摘 要................................................................................................................I
Abstract.............................................................................................................II
謝 誌..............................................................................................................III
目 錄.............................................................................................................IV
表目錄............................................................................................................VII
圖目錄............................................................................................................VII
第1章 緒論....................................................................................................1
1.1 前言...................................................................................................1
1.2 文獻探討...........................................................................................4
1.2.1 定域表面電漿共振(LPR).....................................................4
1.2.2 鎖相放大器應用於生物感測器.........................................10
1.3 研究動機.........................................................................................14
1.4 研究目的.........................................................................................14
第2章 研究方法..........................................................................................15
2.1 實驗材料與設備.............................................................................15
2.2 研究步驟.........................................................................................16
2.3 訊號處理分析.................................................................................23
2.3.1 鎖相放大器原理.................................................................23
2.2.2 鎖相迴路原理.....................................................................32
2.3.3 RC濾波電路.......................................................................39
2.4 實驗流程.........................................................................................41
2.4.1 溶液的配製.........................................................................41
2.4.2 實驗步驟.............................................................................41
第3章 結果與討論......................................................................................43
3.1 試管型LPR生物感測器訊號量測.................................................43
3.2 LM565鎖相迴路RC濾波電路分析..............................................52
3.3 試管型LPR利用LM565鎖相迴路RC濾波電路處理量測訊 號......................................................................................................56
3.4 試管型LPR利用LM565鎖相迴路RC濾波電路匹配探討...........62
3.5 AD620鎖相放大RC濾波電路分析.............................................. 67
3.6 試管型LPR利用AD630鎖相放大RC濾波電路處理量測訊號71
3.7 試管型LPR利用AD630鎖相放大迴路RC濾波電路匹配探討
........................................................................................................77
第4章 結論與未來展望.......................................82
参考文獻..........................................................................................................84
作者簡介.........................................................................................................89

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