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研究生:傅子玲
研究生(外文):Tzu-Ling Fu
論文名稱:台灣南部地區反芻動物類鼻疽桿菌之分子流行病學調查
論文名稱(外文):Investigation of Molecular Epidemiology of Ruminant Melioidosis in Southern Taiwan Area
指導教授:廖明輝廖明輝引用關係
指導教授(外文):Ming-Huei Liao
學位類別:碩士
校院名稱:國立屏東科技大學
系所名稱:獸醫學系所
學門:獸醫學門
學類:獸醫學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:64
中文關鍵詞:類鼻疽伯克氏類鼻疽桿菌聚合酶連鎖反應細菌分離
外文關鍵詞:MelioidosisBurkholderia pseudomalleiPCRbacteria culture
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論文摘要內容:
實驗目的為調查台灣南部地區反芻動物感染類鼻疽的情形,另比較細菌分離與PCR方法偵測羊類鼻疽之效果。本實驗以逢機採集台灣南部地區牛、羊之血液檢體,建立16-23S rRNA之特定ITS序列進行PCR檢測伯克霍德菌 ( Burkholderia pseudomallei ) 核酸抗原,並分析其核酸序列與親緣關係,另以凝集試驗偵測類鼻疽抗體。將送檢25例疑似羊類鼻疽病例組織檢體,進行傳統細菌分離,並抽取組織病原核酸後以PCR偵測。在台灣南部地區平均抗原陽性率與場陽性率於牛隻為9.3 % ( 59/634 ),44.7 % ( 34/76 ) ;羊隻為15.96 % ( 99/620 ),42.5 % ( 34/80 )。定序結果本研究分離株與GeneBank美國株668、1106a、1710b 16-23S rRNA之ITS基因相似度為100 %與99.7 %,以凝集試驗偵測病原血清抗體皆為陰性,以細菌分離為羊類鼻疽金標準診斷法,PCR敏感性為54/78 ( 69 % ),特異性為13/13 ( 100 % )。結果顯示台灣南部反芻類動物以PCR試驗偵測其盛行率頗高,於確診病例中,以肺臟、縱膈淋巴結、睪丸、乳房之細菌分離率及病原核酸偵測率為最高,而診斷羊類鼻疽病例時,仍以傳統細菌分離法有較高之檢出率。


關鍵詞:類鼻疽,伯克氏類鼻疽桿菌,聚合酶連鎖反應,細菌分離。

The contents of abstract in this thesis:
The purpose of this study was to investigation of ruminant melioidosis in southern Taiwan area, and to compare the detective efficacy of melioidosis between bacterial culture and PCR test in organs on goats. We randomly collected blood samples of cattle and goats from southern Taiwan. We then established a PCR protocol for Burkholderia pseudomallei 16-23S rRNA interspacer gene and analyzed its nucleic acid sequences and phylogeneic correlations then detected melioidosis antibody by agglutination. We collected 25 suspect cases of meliodiosis in goat, isolated bacteria by culture and at the same time extracted DNA for detection of pathogenic antigen by PCR assay. The surveillance melioidosis by PCR indicated positive rates and farms positive rates of 9.3 % ( 59/634 ) and 44.7 % ( 34/76 ) in cattle;15.59 % ( 99/620 ) and 42.5 % ( 34/80 ) in goats. After comparing nucleic acid sequences of this study strain with published strains on the GeneBank, the Taiwan strain with American strain 668, 1106a, 1710b 16-23S rRNA ITS have 100 % and 99.7 % identity. The antibody titer of B. pseudomallei was negative by agglutination test. The culture method is considered as the golden standard for detection of B. pseudomallei in goat. The PCR sensitivity was 54/78 ( 69 % ), specificity was 13/13 ( 100 % ) . Results indicating that the prevalence of ruminant melioidosis was higher in southern Taiwan area. In case of confirmed results, lungs, lymph nodes, testis and breast had higher detection ratio both by bacterial culture and PCR.The bacterial culture for diagnosis of melioidosis in goat has higher examination ratio.
Key words: Melioidosis, Burkholderia pseudomallei, PCR, bacteria culture.

目錄
中文摘要.................................................I
英文摘要........ ........................................II
謝誌....................................................IV
目錄.................................................... V
圖表目錄.................................................VII
第1章 前言.............................................. 1
第2章 文獻探討.......................................... 3
2.1 伯克氏類鼻疽桿菌之歷史背景與命名....................... 3
2.2 伯克氏類鼻疽桿菌的特性............................... 3
2.3 類鼻疽桿菌的傳染模式................................. 3
2.4 類鼻疽桿菌致病性..................................... 4
2.5 類鼻疽之流行病學..... ................................5
2.5.1 人類鼻疽之流行病學................................. 5
2.5.2 動物類鼻疽之流行病學............................... 7
2.6 影響伯克氏類鼻疽桿菌之環境因子........................ 8
2.7 類鼻疽之臨床病變..................................... 9
2.7.1 人類鼻疽之臨床病變................................. 9
2.7.2 動物類鼻疽之臨床病變............................... 9
2.8 類鼻疽之治療........................................10
2.9 伯克氏類鼻疽桿菌檢驗方法.............................. 11
2.9.1 抗原的偵測........................................ 11
2.9.2 抗體的偵測 ........................................11
2.9.3 聚合酶連鎖反應診斷法................................12
第3章 材料與方法.........................................16
3.1 檢體來源............................................ 16
3.2 檢體樣本前處理.......................................17
3.3 DNA模板製備......................................... 17
3.4 PCR反應添加物與反應條件.............................. 17
3.5 PCR檢測基因之敏感性試驗.............................. 19
3.6 PCR檢測基因之特異性試驗.............................. 19
3.7 載體之選擇.......................................... 19
3.8 DNA純化............................................ 20
3.9 接合作用............................................ 20
3.10 勝任細胞 ( TOP 10F’ ) 之製作....................... 21
3.11 轉形作用........................................... 21
3.12 菌落篩選........................................... 22
3.13 限制酶切割確認重組之質體DNA.......................... 22
3.14 類鼻疽伯克氏菌屬ITS基因檢測片段之選殖與序列比對分析..... 22
3.15 直接凝集試驗....................................... 23
3.16 細菌分離........................................... 23
3.17 統計方法........................................... 23
第4章 結果............................................... 24
4.1 PCR檢測基因之敏感性試驗電泳圖......................... 24
4.2 PCR檢測基因之特異性試驗電泳圖......................... 25
4.3 PCR檢測臨床血液核酸電泳圖............................ 26
4.4 PCR檢測臨床血液核酸陽性率............................ 27
4.5 PCR測定臨床血液核酸之場陽性率......................... 29
4.6 選殖戴體之構築電泳圖................................. 31
4.7 類鼻疽伯克氏菌屬ITS基因序列比對分析與親緣關係樹狀圖...... 32
4.8 細菌凝集檢測臨床血清抗體.............................. 35
4.9 細菌凝集檢測臨床血清抗體之力價分佈..................... 37
4.10 PCR陽性率與抗體力價之相關性.......................... 39
4.11 羊類鼻疽臟器PCR與細菌分離之比較...................... 39
4.12 羊類鼻疽各臟器PCR與細菌分離之陽性率................... 44
4.13 PCR偵測羊類鼻疽臟器之敏感性與特異性................... 45
第5章 討論.............................................. 46
參考文獻................................................ 50
附錄.................................................... 63
作者簡介................................................ 64

參考文獻

林世傑。類鼻疽致病菌 ( Burkholderia pseudomallei ) 的實驗室診斷。醫檢會報20: 1-7。2005。

陳亞雷、陳垚生、射淵忠、蕭宇珊。南台灣地區類鼻疽菌之地域分佈調查。行政院衛生署疾病管制局科技研究報告。2006。

陳文烈、魯懿萍、蔡睦宗、李明廉、黃旭田、洪信雄。病例報告:羊類鼻疽感染症。台灣省畜牧獸醫學會九十六年度秋季學術研討會。P56。2006。

Anuntagool A, Intachote P, Naigowit P, Sirisinha S. Rapid antigen detection assay for identification of Burkholderia ( Pseudomonas ) pseudomallei infection. J Clin Microbiol 34: 975-976, 1996.

Anuntagool N, Aramsri P, Panichakul T, Wuthiekanun VR, Kinoshita R, White NJ, Sirisinha S. Antigenic heterogeneity of lipopolysaccharide among Burkholderia pseudomallei clinical isolates. Southeast Asian J Trop Med Public Health 31:146-152, 2000.

Ashdown LR. Relationship and significance of specific immunoglobulin M antibody response in clinical and subclinical melioidosis. J Clin Microbiol 14:361-364, 1981.

Brett PJ, DeShazer D, Woods DE. Burkholderia thailandensis sp. nov, a Burkholderia pseudomallei-like species. Int J Syst Bacteriol 48:317-320, 1998.

Brook MD, Currie B, Desmarchelier PM. Isolation and identification of Burkholderia pseudomallei from soil using selective culture techniques and the polymerase chain reaction. J Appl Microbiol 82:589-596, 1997.

Brown AE, Dance DA, Chaowagul W, Webster HK, White NJ. Activation of cellular immune responses in melioidosis patients as assessed by urinary neopterin. Trans R Soc Trop Med Hyg 84:583-584, 1990.

Chantratita N, Meumann E, Thanwisai A, Limmathurotsakul D, Wuthiekanun V, Wannapsni S, Tumapa S, Day NPJ, Peacock SJ. Loop-mediated isothermal amplification methods targeting the TTS1 gene cluster for detection of Burkholderia pseudomallei and diagnosis of melioidosis. J Clin Microbial 46:568-573, 2008.

Chen YH, Peng CF, Hwang KP, Tsai JJ, Lu PL, Chen TP. An indigenous melioidosis: a case report. Kaohsiung J Med Sci 15:292-296, 1999.

Cheng AC, Currie BJ. Melioidosis: epidemiology, pathophysiology, and management. Clin Microbiol Rev 18:383-416, 2005.

Cheng AC, O'brien M, Freeman K, Lum G, Currie BJ. Indirect hemagglutination assay in patients with melioidosis in northern Australia. Am J Trop Med Hyg 74:330-334, 2006.

Choy J, Mayo M, Janmaat A, Currie BJ. Animal melioidosis in Australia. Acta Trop 74: 153-158, 2000.

Currie BJ, Fisher DA, D. Howard M, Burrow J N. Neurological melioidosis. Acta Trop 74:145-151, 2000.

Currie BJ, Fisher DA, Anstey NM. Melioidosis: acute and chronic disease, relapse and re-activation. Trans R Soc Trop Med Hyg 94: 301-304, 2000.

Currie BJ, Mayo M, Anstey NM, Donohoe P, Haase A, Kemp DJ. A cluster of melioidosis cases from an endemic region is clonal and is linked to the water supply using molecular typing of Burkholderia pseudomallei isolates. Am J Trop Med Hyg 65:177-179, 2001.

Currie BJ, Jacups SP. Intensity of rainfall and severity of melioidosis, Australia. Emerg Infect Dis 9:538-542, 2003.

Cuzzubbo AJ, Chenthamarakshan V, Vadivelu J, Puthucheary SD, Rowland D, Devine PL. Evaluation of a new commercially available immunoglobulin M and immunoglobulin G immunochromatographic test for diagnosis of melioidosis infection. J Clin Microbiol 38:1670-1671, 2000.

Chaowagul W, White NJ, Dance DA, Wattanagoon Y, Naigowit P, Davis TM, Looareesuwan S, Pitakwatchara N. Melioidosis: a major cause of community-acquired septicemia in northeastern Thailand. J Infect Dis 159:890-899, 1989.

Chaowagul W, Suputtamongkol Y, Dance DA, Rajchanuvong A, Pattara-arechachai J, White NJ. Relapse in melioidosis: incidence and risk factors. J Infect Dis 168:1181-1185, 1993.

Charoenwong P, Lumbiganon P, Puapermpoonsiri S. The prevalence of the indirect hemagglutination test for melioidosis in children in endemic area. Southeast Asian J Trop Med Pub Health 23:698-701, 1992.

Dance DA, Wuthiekanun, Naigowit P, White NJ. Identification of Pseudomonas pseudomallei in clinical practice: use of simple screening tests and API 20NE. J Clin Pathol 42: 645-648, 1989.

Dance DA. Ecology of Burkholderia pseudomallei and the interactions between environmental Burkholderia spp. and human-animal hosts. Acta Trop 74: 159-168, 2000.

Dharakul T, Songsivilai S, Anuntagool N, Chaowagul W, Wongbunnate S, Intachote P, Sirisinha S. Diagnostic value of an antibody enzyme linked immunosorbent assay using affinity-purified antigen in an area endemic for melioidosis. Am J Trop Med Hyg 56:418-423, 1997.

Freeman K, Lum G, Boer JD. Presented at the Australian Society of Microbiology Cairns, 2000.

Gee JE, Sacchi CT, Glass MB, De BK, Weyant RS, Levett PN, Whitney AM, Hoffmaster AR, Popovic T. Use of 16S rRNA gene sequencing for rapid identification and differentiation of Burkholderia pseudomallei and B. mallei. J Clin Microbiol 41:4647-4654, 2003.

Gibbons HS, Lin S, Cotter RJ, Raetz CR. Oxygen requirementfor the biosynthesis of the S-2-hydroxymyristate moiety in Salmonella typhimurium lipid A. J Biol Chem 275:32940-32949, 2000.

Godoy D, Randle G, Simpson AJ, Aanensen DM, Pitt TL, Kinoshita R, and Spratt BG. Multilocus sequence typing and evolutionary relationships among the causative agents of melioidosis and glanders, Burkholderia pseudomallei and Burkholderia mallei. J Clin Microbiol 41:2068-2079, 2003.

Haase A, Brennan M, Barrett S, Wood Y, Huffam S, Brien DO, Currie B. Evaluation of PCR for diagnosis of melioidosis. J Clin Microbiol 36:1039-1041, 1998.

Hoppe I, Brenneke B, Rohde M, Kreft A, Haussler S, Reganzerowski A, Steinmetz I. Characterization of a murine model of melioidosis: comparison of different strains of mice. Infect Immun 67:2891-2900, 1999.

Holden MT, Titball RW, Peacock SJ, Cerdeno-Tarraga AM, Atkins T, Crossman LC, Pitt T, Churcher C, Mungall K, Bentley SD, Sebaihia M, Thomson NR, Bason N, Beacham IR, Brooks K, Brown KA, Brown NF, Challis GL, Cherevach I, Chillingworth T, Cronin A, Crossett B, Davis P, DeShazer D, Feltwell T, Fraser A, Hance Z, Hauser H, Holroyd S, Jagels K, Keith KE, Maddison M, Moule S, Price C, Quail MA, Rabbinowitsch E, Rutherford K, Sanders M, Simmonds M, Songsivilai S, Stevens K, Tumapa S, Vesaratchavest M, Whitehead S, Yeats C, Barrell BG, Oyston PC, and Parkhill J. Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei. Proc Natl Acad Sci USA 101:14240-14245, 2004.

Howard K and Inglis TJJ.Novel Selective Medium for Isolation of Burkholderia pseudomallei. J Clin Microbiol 41:3312-3316, 2003.

Howe C, Sampath A, Spotnitz M. The pseudomallei group: a review. J Infect Dis 124:598-606, 1971.

Holtze MW. The capabilities of the Northern Territory for tropic agriculture. Adelaide Work J:17-27, 1901.

Hsueh PR, Teng LJ, Lee LN, Yu CJ, Yang PC, Ho SW, Luh KT. Melioidosis: an emerging infection in Taiwan?. Emerg Infect Dis 7 428-433, 2001.

Ileri S. The indirect haemagglutination tests in the diagnosis of melioidosis in goats. Br Vet J 121:164-170, 1965.

Ismail G, Razak N, Mohamed R, Embi N, Omar O. Resistance of Pseudomonas pseudomallei to normal human serum bactericidal action. Microbiol Immunol 32:645-652, 1988.

Inglis TJ, Garrow SC, Adams C, Henderson M, Mayo M, Currie BJ. Acute melioidosis outbreak in Western Australia. Epidemiol Infect 123:437-443, 1999.

Inglis TJ, Aravena-Roman M, Ching S, Croft K, Wuthiekanun V, Mee BJ. Cellular fatty acid profile distinguishes Burkholderia pseudomallei from avirulent Burkholderia thailandensis. J Clin Micbiol 41:4812-4814, 2003.

Inglis TJJ, Merritt A, Chidlow G, Aravena-Roman M, Harnett G. Comparison of Diagnostic Laboratory Methods for Identification of Burkholderia Pseudomallei. J Clin Micbiol 43: 2201-2206, 2005.

Inglis TJJ and Sagripanti JE. Environmental Factors That Affect the Survival and Persistence of Burkholderia pseudomallei. Applied and environment microbiology 72:6865-6875, 2006.

Kanaphun P, Thirawattanasuk N, Suputtamongkol Y, Naigowit P, Dance DA, Smith MD, White NJ. Serology and carriage of Pseudomonas pseudomallei: a prospective study in 1000 hospitalized children in northeast Thailand. J Infect Dis 167:230-233, 1993.

Ketterer PJ, Donald B, Rogers RJ. Bovine melioidosis in South-Eastern Queensland. Aust Vet J 51: 395-398, 1975.

Khupulsup K, Petchclai B. Application of indirect hemagglutination test and indirect fluorescent antibody test for IgM antibody for diagnosis of melioidosis in Thailand. Am J Trop Med Hyg 35:366-369, 1986.

Kunakorn M, Jayanetra P, Tanphaichitra D. Man-to-man transmission of melioidosis. Lancet 337:1290-1291, 1991.

Kunakorn M, Raksakait K, Sethaudom C, Sermswan RW, Dharakul T. Comparison of three PCR primer sets for diagnosis of septicemic melioidosis. Acta Trop 74:247-251, 2000.

Laws L, Hall WTK. Melioidosis in animals in north Queensland. Qld J Agric Sci 20:499-513, 1963.

Lee N, Wu JL, Lee CH, Tsai WC.Pseudomonas pseudomallei infection from drowning: the first reported case in Taiwan. J Clin Microbiol 22:352-354, 1985.

Lee SS, Liu YC, Chen YS, Wann SR, Wang JH, Yen MY, Wang JH, Lin HH, Huang WK, Cheng DL. Melioidosis: two indigenous cases in Taiwan. J Formos Med Assoc 95:562-566, 1996.

Lee SH, Chong CE, Lim BS, Chai SJ, Sam KK, Rahmah M, Sheila N. Burkholdeira pseudomallei animal and human isolates from Malaysia exhibit different phenotypic characteristics. Dia Microbio Infec Dis 58: 263-270, 2007.

Leelarasamee A, Bovornkitti S. Melioidosis: review and update. Rev Infect Dis 11:413-425, 1989.

Liang SL, Liang H. Pulmonary melioidosis. Report of 4 cases and literature reviewed. Zhonghua Jie He He Hu Xi Za Zhi. 17:168-170, 1994.

Li L, Lu Z, Han O. Epidemiology of melioidosis in China. Zhonghua Jie He He Hu Xi Za Zhi 17:168-170, 1994.

Luo CY, Ko WC, Lee HC, Yang YJ. Relapsing melioidosis as cause of iliac mycotic aneurysm: an indigenous case in Taiwan. J Vasc Surg 37:882-885, 2003.

McCormick JB, Sexton DJ, McMurray JG, Carey E, Hayes P, Feldman RA. Human-to-human transmission of Pseudomonas pseudomallei. Ann Intern Med 83:512-513, 1975.

Naigowit P, Kurata T, Wangroongsub P, Petkanjanapong V, Kondo E, Kanai K. Application of indirect immunofluorescence microscopy to colony identification of Pseudomonas pseudomallei. Asian Pac J Allergy Immunol 11:149-154, 1993.

Nierman WC, DeShazer D, Kim HS, Tettelin H, Nelson KE, Feldblyum T etc. Structural flexibility in the Burkholderia mallei genome. Proc Natl Acad Sci USA 101:14246-14251, 2004.

O’Brien M, Freeman K, Lum G, Cheng AC, Jacups SP, Currie BJ. Further evaluation of a rapid diagnostic test for melioidosis in an area of endemicity. J Clin Microbiol 42:2239-2240, 2004.

Parry CM, Wuthiekanun V, Hoa NT, Diep TS, Thao LT, Loc PV, Wills BA, Wain J, Hien TT, White NJ, Farrar JJ. Melioidosis in Southern Vietnam: clinical surveillance and environmental sampling. Clin Infect Dis 29:1323-1326, 1999.

Pitt TL, Pseudomonas, Burkholderia and related genera, In W. J. Haussler and M. Sussman (ed.), Topley and Wilson’s microbiology and microbial infections 2:1109-1138. 1995.

Puthucheary SD, Parasakthi N, Lee MK. Septicaemic melioidosis: a review of 50 cases from Malaysia.Trans R Soc Trop Med Hyg 86:683-685, 1992.

Rattanathongkom A, Sermswan RW, Wongratanacheewin S. Detection of Burkholderia pseudomallei in blood samples using polymerase chain reaction. Mol Cell Probes 11:25-31, 1997.

Romagnani S. Biology of human TH1 and TH2 cells. J Clin Immunol 15:121-129, 1995.

Russell P, Eley SM, Ellis J, Green M, Bell DL, Kenny DJ, Titball RW. Comparison of efficacy of ciprofloxacin and doxycycline against experimental melioidosis and glanders. J Antimicrob Chemother 45:813-818, 2000.

Sermswan RW, Wongratanacheewin S, Anuntagool N, Sirisinha S. comparison of the polymerase chain reaction and serologic tests for diagnosis of septicemic melioidosis. Am J Trop Med Hyg 63:146-149, 2000.

Simpson AJ, Howe PA , Wuthiekanun V, and White NJ. A comparison of lysis centrifugation, pour plate, and conventional blood culture methods in the diagnosis of septicaemic melioidosis. J Clin Pathol 52:616-619, 1999.

Sirisinha S, Anuntagool N, Intachote P, Wuthiekanun V, Puthucheary SD, Vadivelu J, White NJ. Antigenic differences between clinical and environmental isolates of Burkholderia pseudomallei. Microbiol Immunol 42:731-737, 1998.

Sirisinha S, Anuntagool N, Dharakul T, Ekpo P, Wongratanacheewin S, NaigowitP, Petchclai B, Thamlikitkul V, Suputtamongkol Y. Recent developments in laboratory diagnosis of melioidosis. Acta Trop 74:235-245, 2000.

Smith MD, Angus BJ, Wuthiekanun V, White NJ. Arabinose assimilation defines a nonvirulent biotype of Burkholderia pseudomallei. Infect Immun 65:4319-4321, 1997.

Smith Vaughan HC, Gal D, Lawrie PM, Winstanley C, Sriprakash KS, Currie BJ. Ubiquity of putative type III secretion genes among clinical and environmental Burkholderia pseudomallei isolates in Northern Australia. J Clin Microbiol 41:883-885, 2003.

Sookpranee M, Boonma P, Susaengrat W, Bhuripanyo K, Punyagupta S.
Multicenter prospective randomized trial comparing ceftazidime plus co-trimoxazole with chloramphenicol plus doxycycline and co-trimoxazole for treatment of severe melioidosis. Antimicrob Agents Chemother 36:158-162, 1992.

Sprague LD, Neubauer H. Melioidosis in animals: a rewiew on epizootiology, diagnosis and clinical presentation. J Vet Med 51: 305-320, 2004.

Steinmetz I, Stosiek P, hergenrother D, Bar W. Melioidosis causing a mycotic aneurysm. Lancet 347 1564-1565, 1996.

Steinmetz I, Reganzerowski A, Brenneke B, Haussler S, Simpson A, White NJ. Rapid identification of Burkholderia pseudomallei by latex agglutination based on an exopolysaccharide-specific monoclonal antibody. J Clin Microbiol 37:225-228, 1999.

Sutmoller P, Kraneveld FC, Vander S. A Melioidosis (Pseudomalleus) in sheep, goats, and pigs on Aruba (Netherland Antilles). J Am Vet Med Assoc 130:415-417, 1957.

Supaprom C, Wang D, Leelayuwat C, Thaewpia W, Susaengrat W, Koh V, Ooi EE, Lertmemongkolchai G, Liu Y. Development of real-time PCR assays and evaluation of their potential use for rapid detection of Burkholderia pseudomallei in clinical blood specimens.J Clin Microbiol 45: 2894-2901, 2007.

Suputtamongkol Y, Hall AJ, Dance DA, Chaowagul W, Rajchanuvong A, Smith MD, White NJ. The epidemiology of melioidosis in Ubon Ratchatani, northeast Thailand. Int J Epidemiol 23:1082-1090, 1994.

Sura T, Smith MD, Cowan GM, Walsh AL, White NJ, Krishna S. Polymerase chain reaction for the detection of Burkholderia pseudomallei. Diagn Microbiol Infect Dis 29:121-127, 1997.

Thomas A, Forbes-Faulkner JC. Persistence of Pseudomonas pseudomallei in soil. Aust Vet J 57:535-536, 1981.

Thomas AD, Forbes-Faulkner JC, Norton JH, Trueman KF. Clinical and pathological observations on goats experimentally infected with pseudomonas pseudomallei. Aust Vet J 65: 43-46, 1988.

Tiangpitayakorn C, Songsivilai S, Piyasangthong N, Dharakul T. Speed of detection of Burkholderia pseudomallei in blood cultures and its correlation with the clinical outcome. Am J Trop Med Hyg 57:96-99, 1997.

Ulett GC, Ketheesan N, Hirst RG. Cytokine gene expression in innately susceptible BALB/c mice and relatively resistant C57BL/6 mice during infection with virulent Burkholderia pseudomallei. Infect Immun 68:2034-2042, 2000.

Van der Lugt JJ, Henton MM. Melioidosis in a goat. J S Afr Vet Assoc 66: 71-73, 1995.

Walsh AL, Smith MD, Wuthiekanun V, Suputtamongkol Y, Desakorn V, Chaowagul W, White NJ. Immunofluorescence microscopy for the rapid diagnosis of melioidosis. J Clin Pathol 47:377-379, 1994.

Walsh AL, Smith MD, Wuthiekanun V, Suputtamongkol Y, Chaowagul W, Dance DA, Angus B, White NJ. Prognostic significance of quantitative bacteremia in septicemic melioidosis. Clin Infect Dis 21:1498-1500, 1995.

White NJ. Melioidosis. Lancet 361:1715-1722, 2003.

Woo PC, Lau SK, Woo GW, Fung AM, Ngan AH, Hui WT, Yuen KY. Seronegative bacteremic melioidosis caused by Burkholderia pseudomallei with ambiguous biochemical profile: clinical importance of accurate identification by 16S rRNA gene and groEL gene sequencing. J ClinMicrobiol 41:3973-3977, 2003.

Wongratanacheewin S, Tattawasart U, Lulitanond V, Wongwajana S, ermswan RW, Sookpranee M, Nuntirooj K. Characterization of Pseudomonas pseudomallei antigens by SDS-polyacrylamide gel electrophoresis and western blot. Southeast Asian J Trop Med Public Health. 24:107-113, 1993.

Wuthiekanun V, Amornchai P, Chierakul W, Cheng AC, White NJ, Peacock SJ, Day NP. Evaluation of immunoglobulin M (IgM) and IgG rapid cassette test kits for diagnosis of melioidosis in an area of endemicity. J Clin Microbiol 42:3435-3437, 2004.

Yang S. Melioidosis research in China. Acta Trop 77:157-165, 2000.

Yabuuchi E, Kosako Y, Oyaizu H, Yano I, Hotta H, Hashimoto Y, Ezaki T, Arakawa M. Proposal of Burkholderia gen. nov. and transfer of seven species of the genus Pseudomonas homology group II to the new genus, with the type species Burkholderia cepacia. Microbiol Immu¬nol. 36:1251-1275, 1992.

Yee KC, Lee MK, Chua CT, Puthucheary SD. Melioidosis, the great mi-micker: a report of 10 cases from Malaysia. J Trop Med Hyg 91:249-254,1988.


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