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研究生(外文):Yi-chi Huang
論文名稱(外文):Differential responses of peroxidases during the formation of adventitious root in hypocotyl cuttings of soybean
指導教授(外文):Zin-huang Liu
外文關鍵詞:adventitious rootGlycine maxauxinperoxidase
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外加植物生長素(IAA, IBA, NAA)可以促進大豆下胚軸誘發不定根,不同的植物生長素促進發根的能力也有所不同,其中又以Indole-3-butyric acid (IBA)促進大豆下胚軸誘發不定根的能力最好。在不定根發育的誘導期(induction phase),外加的生長素會抑制陰性(pI 3.7) 和陽性 (pI 8.5) 過氧化酶活性的表現。而在不定根發育的末期,在外加IBA的大豆下胚軸中,pI 5.3過氧化酶活性會大幅表現,同時,過氧化氫的含量也有明顯上升的趨勢。之前的研究發現pI 8.5過氧化酶基因的啟動子含有兩個auxin response element (ARF/AuxRE和CATATGGMSAUR)。我的研究則是發現在pI 5.3 過氧化酶基因啟動子有一個auxin response element (ARF/AuxRE)。因此推測pI 5.3 過氧化酶可能和不定根形成的末期有關。pI 5.3 過氧化酶會藉由與auxin的結合產生過氧化氫,並調控auxin的訊息傳遞路徑來影響植物的生長。
The auxins, including indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and naphthaleneacetic acid (NAA), promotes the formation of adventitious root in hypocotyls of soybean (Glycine max). IBA significantly promotes the formation of adventitious roots more than IAA and NAA . The activity of anionic pI 3.7 peroxidase (POX) and cationic pI 8.5 POX were inhibited by exogenous auxins during the induction of adventitious root on day 2. Besides, the activity of pI 5.3 POX was enhanced by IBA during the initiation stage on day 4. The increase of the activity of pI 5.3 POX was accompanied by the increase of H2O2 levels. In the previous researches, it shows that the promoter of pI 8.5 POX gene contains both ARF/AuxRE and CATATGGMSAUR motifs that are responded to auxins. In this studies, the pI 5.3 POX gene, which responses to auxins a day or two later, contains only ARF/AuxRE motif. The regulation of pI 5.3 POX gene is probably initiation phase-dependent. The results suggest that anionic pI 5.3 POX produces significant amount of H2O2 through the binding of auxin to POX and mediate the auxin signaling pathway leading to plant growth .
Chinese abstract……………………………………….2
English abstract………………………………………..3
Table of contents………………………………….……4
List of tables and figures………………………………5
Materials and methods……………………..…………10
Tables and figures……………………………………...33
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