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研究生:吳建德
研究生(外文):Wu, Jian-De
論文名稱:研究果蠅行為的即時影像與雷射追跡系統之研發
論文名稱(外文):Vision Based Real-Time Laser Tracking System for the Investigation of Drosophila Behaviors
指導教授:傅建中傅建中引用關係
指導教授(外文):Fu, Chien-Chung
學位類別:碩士
校院名稱:國立清華大學
系所名稱:動力機械工程學系
學門:工程學門
學類:機械工程學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:59
中文關鍵詞:果蠅嗅覺行為即時影像處理
外文關鍵詞:DrosophilaOlfactory BehaviorReal - timeImage processing
相關次數:
  • 被引用被引用:3
  • 點閱點閱:180
  • 評分評分:
  • 下載下載:6
  • 收藏至我的研究室書目清單書目收藏:0
使用傳統方法進行果蠅嗅覺實驗研究已經解開巨觀上神經網絡之謎,然而受限於實驗方法及輔助工具的不足,勢必要通過更複雜的實驗設計才能一窺微觀尺度的細部神經網絡連結,因此為了有效化簡實驗設計並加速研究基因篩選,本研究提出了一項可結合雷射進行ChR2實驗,應用於研究單隻果蠅於二維空間下的嗅覺行為趨勢即時觀測系統。透過特殊設計的果蠅盒,我們不需剪去果蠅翅膀即可限制其於近乎二維方向上移動,同時我們利用機器視覺的方式擷取果蠅影像,在此應用於機器視覺照明的光源亦不影響ChR2實驗的進行,由於特殊的果蠅治具及相關硬體設置,經由簡單的影像前處理及果蠅辨認步驟,即可得到穩定的果蠅位置資訊,藉由位置資訊進行基本的行為參數定義,最後透過程式執行程序、時序的安排達成即時觀測系統的需求。除了即時觀測模組外,我們亦於程式中加入離線分析模組可進行實驗影片錄製以及果蠅趨勢圖繪製。期望透過我們的系統能夠幫助建立新型態的果蠅行為趨勢研究,並有效率加速相關實驗及研究。
圖目錄 II
表目錄 IV
第一章 緒論 1
前言 1
1-1 研究背景 2
1-2 研究動機及目的 6
1-3 文獻回顧 7
1-4 論文組織及概要 11
第二章 系統架構與設計 12
2-1 系統設計概念 12
2-2 硬體組成規劃 13
2-2-1影像擷取暨處理設備 14
2-2-2 果蠅治具(Arena) 15
2-2-3 照明 16
2-3軟體設計、架構 20
2-3-1 軟體功能需求 20
2-3-2 LabVIEW 程式分析 22
2-3-3 介面設計、程式架構 22
2-3-4 程式處理流程 26
2-3-5 程序、時序安排 42
第三章 實驗討論 47
第四章 結論與未來工作 53
參考文獻 55
[1] D. Stipp, "Grand gamble on fruit fly learning," Science, vol. 253, pp. 1486-7, Sep 27 1991.

[2] National Human Genome Research Institute. [Online]. Available in:
http://www.genome.gov/

[3] M. Hammer and R. Menzel, "Learning and memory in the honeybee," J Neurosci, vol. 15, pp. 1617-30, Mar 1995.

[4] R. Menzel and U. Muller, "Learning and memory in honeybees: from behavior to neural substrates," Annu Rev Neurosci, vol. 19, pp. 379-404, 1996.

[5] E. A. Capaldi, G. E. Robinson, and S. E. Fahrback, "Neuroethology of spatial learning: the birds and the bees," Annu Rev Psychol, vol. 50, pp. 651-82, 1999.

[6] J. M. Camhi and T. G. Nolen, "Properties of the Escape System of Cockroaches During Walking," J Comp Physiol [A], vol. 142, pp. 339-346, 1981.

[7] M. Wenner﹐基因療法再顯聲威 ﹐科學人雜誌﹐2008

[8] 嚴震東﹐系統神經科學的研究方法 ﹐科學月刊﹐1993

[9] Neuroscience For Kids. [Online]. Available in:
http://faculty.washington.edu/chudler/neurok.html

[10] 演化的證據.[Online]. Available in :
http://vschool.scu.edu.tw/biology/content/genetics.htm


[11] L. B. Vosshall, "Olfaction in Drosophila," Curr Opin Neurobiol, vol. 10, pp. 498-503, Aug 2000.

[12] R. L. Davis, "Olfactory learning," Neuron, vol. 44, pp. 31-48, Sep 30 2004.

[13] C. J. Reaume and M. B. Sokolowski, "The nature of Drosophila melanogaster," Curr Biol, vol. 16, pp. R623-8, Aug 22 2006.

[14] 陳雅茜譯﹐果蠅—閃亮的生命科學研究先驅﹐天下文化﹐2003

[15] N. K. Tanaka, T. Awasaki, T. Shimada, and K. Ito, "Integration of chemosensory pathways in the Drosophila second-order olfactory centers," Curr Biol, vol. 14, pp. 449-57, Mar 23 2004.

[16] S. Libert, J. Zwiener, X. Chu, W. Vanvoorhies, G. Roman, and S. D. Pletcher, "Regulation of Drosophila life span by olfaction and food-derived odors," Science, vol. 315, pp. 1133-7, Feb 23 2007.

[17] L. B. Vosshall and R. F. Stocker, "Molecular architecture of smell and taste in Drosophila," Annu Rev Neurosci, vol. 30, pp. 505-33, 2007.

[18] T. Tully and W. G. Quinn, "Classical conditioning and retention in normal and mutant Drosophila melanogaster," J Comp Physiol [A], vol. 157, pp. 263-77, Sep 1985.

[19] T. Tully, "Pavlov's dogs," Current Biology, vol. 13, pp. R117-R119, 2003.

[20] G. Nagel, T. Szellas, W. Huhn, S. Kateriya, N. Adeishvili, P. Berthold, D. Ollig, P. Hegemann, and E. Bamberg, "Channelrhodopsin-2, a directly light-gated cation-selective membrane channel," Proc Natl Acad Sci U S A, vol. 100, pp. 13940-5, Nov 25 2003.

[21] F. Zhang, L. P. Wang, E. S. Boyden, and K. Deisseroth, "Channelrhodopsin-2 and optical control of excitable cells," Nat Methods, vol. 3, pp. 785-92, Oct 2006.

[22] M. Hausser and S. L. Smith, "Neuroscience: controlling neural circuits with light," Nature, vol. 446, pp. 617-9, Apr 5 2007.
[23] F. Zhang, A. M. Aravanis, A. Adamantidis, L. de Lecea, and K. Deisseroth, "Circuit-breakers: optical technologies for probing neural signals and systems," Nat Rev Neurosci, vol. 8, pp. 577-81, Aug 2007.

[24] DNA from the beginning. [Online]. Available in:http://dna.ym.edu.tw/

[25] R. Wolf and M. Heisenberg, "Basic organization of operant behavior as revealed in Drosophila flight orientation," J Comp Physiol [A], vol. 169, pp. 699-705, Dec 1991.

[26] A. C. Keene, M. Stratmann, A. Keller, P. N. Perrat, L. B. Vosshall, and S. Waddell, "Diverse odor-conditioned memories require uniquely timed dorsal paired medial neuron output," Neuron, vol. 44, pp. 521-33, Oct 28 2004.

[27] J. J. Krupp, C. Kent, J. C. Billeter, R. Azanchi, A. K. So, J. A. Schonfeld, B. P. Smith, C. Lucas, and J. D. Levine, "Social experience modifies pheromone expression and mating behavior in male Drosophila melanogaster," Curr Biol, vol. 18, pp. 1373-83, Sep 23 2008.

[28] I. Ganguly-Fitzgerald, J. Donlea, and P. J. Shaw, "Waking experience affects sleep need in Drosophila," Science, vol. 313, pp. 1775-81, Sep 22 2006.

[29] S. Q. Lima and G. Miesenbock, "Remote control of behavior through genetically targeted photostimulation of neurons," Cell, vol. 121, pp. 141-52, Apr 8 2005.

[30] R. L. Davis, "Remote control of fruit fly behavior," Cell, vol. 121, pp. 6-7, Apr 8 2005.

[31] G. S. Suh, S. Ben-Tabou de Leon, H. Tanimoto, A. Fiala, S. Benzer, and D. J. Anderson, "Light activation of an innate olfactory avoidance response in Drosophila," Curr Biol, vol. 17, pp. 905-8, May 15 2007.

[32] G. Nagel, M. Brauner, J. F. Liewald, N. Adeishvili, E. Bamberg, and A. Gottschalk, "Light activation of channelrhodopsin-2 in excitable cells of Caenorhabditis elegans triggers rapid behavioral responses," Curr Biol, vol. 15, pp. 2279-84, Dec 20 2005.

[33] B. R. Arenkiel, J. Peca, I. G. Davison, C. Feliciano, K. Deisseroth, G. J. Augustine, M. D. Ehlers, and G. Feng, "In vivo light-induced activation of neural circuitry in transgenic mice expressing channelrhodopsin-2," Neuron, vol. 54, pp. 205-18, Apr 19 2007.

[34] A. M. Aravanis, L. P. Wang, F. Zhang, L. A. Meltzer, M. Z. Mogri, M. B. Schneider, and K. Deisseroth, "An optical neural interface: in vivo control of rodent motor cortex with integrated fiberoptic and optogenetic technology," J Neural Eng, vol. 4, pp. S143-56, Sep 2007.

[35] V. Gradinaru, K. R. Thompson, F. Zhang, M. Mogri, K. Kay, M. B. Schneider, and K. Deisseroth, "Targeting and readout strategies for fast optical neural control in vitro and in vivo," J Neurosci, vol. 27, pp. 14231-8, Dec 26 2007.

[36] A. R. Adamantidis, F. Zhang, A. M. Aravanis, K. Deisseroth, and L. de Lecea, "Neural substrates of awakening probed with optogenetic control of hypocretin neurons," Nature, vol. 450, pp. 420-4, Nov 15 2007.

[37] W. Zhang, W. Ge, and Z. Wang, "A toolbox for light control of Drosophila behaviors through Channelrhodopsin 2-mediated photoactivation of targeted neurons," Eur J Neurosci, vol. 26, pp. 2405-16, Nov 2007.

[38] D. Huber, L. Petreanu, N. Ghitani, S. Ranade, T. Hromadka, Z. Mainen, and K. Svoboda, "Sparse optical microstimulation in barrel cortex drives learned behaviour in freely moving mice," Nature, vol. 451, pp. 61-4, Jan 3 2008.

[39] A. D. Douglass, S. Kraves, K. Deisseroth, A. F. Schier, and F. Engert, "Escape behavior elicited by single, channelrhodopsin-2-evoked spikes in zebrafish somatosensory neurons," Curr Biol, vol. 18, pp. 1133-7, Aug 5 2008.

[40] P. Schoenenberger, A. Grunditz, T. Rose, and T. G. Oertner, "Optimizing the spatial resolution of Channelrhodopsin-2 activation," Brain Cell Biol, vol. 36, pp. 119-27, Aug 2008.

[41] MARC D. BINDER, NOBUTAKA HIROKAWA AND UWE WINDHORST (Eds.)﹐Encyclopedia of Neuroscience﹐Springer-Verlag GmbH Berlin Heidelberg﹐2008

[42] D. Fields﹐深植記憶﹐科學人雜誌﹐2005

[43] U. Frey and R. G. Morris, "Synaptic tagging and long-term potentiation," Nature, vol. 385, pp. 533-6, Feb 6 1997.

[44] H. Dankert, L. Wang, E. D. Hoopfer, D. J. Anderson, and P. Perona, "Automated monitoring and analysis of social behavior in Drosophila," Nat Methods, vol. 6, pp. 297-303, Apr 2009.

[45] K. Branson, A. A. Robie, J. Bender, P. Perona, and M. H. Dickinson, "High-throughput ethomics in large groups of Drosophila," Nat Methods, vol. 6, pp. 451-7, Jun 2009.

[46] A. K. C. Wong and P. K. Sahoo, "A gray-level threshold selection method based on maximum entropy principle," Systems, Man and Cybernetics, IEEE Transactions on, vol. 19, pp. 866-871, 1989.

[47] M. Heisenberg, "Mushroom body memoir: from maps to models," Nat Rev Neurosci, vol. 4, pp. 266-75, Apr 2003.

[48] J. Guo and A. Guo, "Crossmodal interactions between olfactory and visual learning in Drosophila," Science, vol. 309, pp. 307-10, Jul 8 2005.

[49] L. B. Vosshall, "Into the mind of a fly," Nature, vol. 450, pp. 193-7, Nov 8 2007.

[50] R.C. Gonzalez & R.E. Woods﹐Digital Image Processing﹐Prentice Hall﹐2002

[51] S. Theodoridis & K. Koutroumbas, Pattern Recognition , Academic Press,2008

[52] A. Silberschatz, G. Gagne, and P.B. Galvin, Operating System Concepts , Wiley, 2005
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