臺灣博碩士論文加值系統

首先觀察18、25、32℃三種溫度下對台灣石斑虹彩病毒 (Grouper iridovirus of Taiwan, TGIV) 增殖的影響，初步以PCR偵測病毒的增殖情形，結果發現18℃、25℃下可以明顯偵測到TGIV，而32℃下則幾乎偵測不到病毒的存在；之後分析以上三個溫度下TGIV之增殖曲線，結果18℃、25℃的細胞相關病毒 (Cell associate virus, CAV) 在感染後16天力價達106∼107 TCID50/ml，32℃只有104.25 TCID50/ml；另外釋放性病毒 (Release virus, RV) 力價以25℃達106.4 TCID50/ml最高，18℃與32℃只有104 TCID50/ml左右。之後以PI與Annexin-V染色經流式細胞儀分析細胞週期與凋亡細胞，發現在32℃高溫感染組於感染後五天，開始出現細胞凋亡的Sub G1時期，第九天時細胞週期中Sub G1波峰更為明顯，數量化後凋亡細胞比例約佔37.5%；而Annexin-V與PI雙重染色則可發現25℃感染組的細胞，隨著感染天數增加逐漸往四象限圖中壞死細胞代表的Q1+Q2位置移動，32℃則逐漸往凋亡細胞代表的Q4象限移動，感染後第九天凋亡比例達24%。另外Annexin-V螢光染色觀察也可以發現18℃與25℃於感染後第三天開始出現PI染色發出紅色螢光的壞死細胞，而32℃感染後第三天則是出現Annexin-V染色發出綠色螢光的凋亡細胞。進一步以西方點墨法 (Western blot) 分析高溫下TGIV感染SB細胞之熱休克蛋白70、抗凋亡蛋白Bcl-2與促凋亡蛋白Bak的表現，結果32℃感染組熱休克蛋白70有明顯表現量，但是在抗凋亡蛋白Bcl-2與促凋亡蛋白Bak的表現都不明顯；相對地，25℃感染組則在抗凋亡Bcl-2蛋白大量表現，但熱休克蛋白70與促凋亡蛋白Bak的表現不明顯。在活體實驗中，32℃病毒攻擊組兩週後的累積死亡率只有37.5%，25℃累積死亡率卻達到100%，將死亡魚隻進行光學組織切片H&E染色與TUNEL染色後觀察，同樣在32℃組呈現較強的細胞凋亡TUNEL陽性反應，顯示高溫下TGIV誘發之細胞凋亡可能與細胞防禦病毒感染機制相關。

The influence of temperature on the Grouper iridovirus of Taiwan (TGIV) multiplication at 18, 25 and 32℃, respectively were investigated in the study. First, PCR results showed TGIV-infected cells at 18 and 25℃ have 1339 bp specific TGIV amplicon but could not be found at 32℃. Then cell-associated virus (CAV) and released virus (RV) at different time points after TGIV inoculation was estimated for determining the growth curve of TGIV. Virus yields of CAV under hyperthermia (32℃) group only 104.25 TCID50/ml, lower than the other two temperature groups of titer 106∼107 TCID50/ml. Furthermore, cell cycles and apoptosis cells were measured by flow cytometric analysis using propidium iodide (PI) and Annexin-Ⅴ. The TGIV-infected cells at 32℃ showed apoptosis in the sub-G1 phase at 5 dpi and reached peak at 9 dpi. And it had 24% apoptosis cells at 9 dpi in Q4 region. The double labeling assay method combines the staining properties of Annexin-Ⅴ and PI was observed by microscopy. The test detected apoptosis-dependent phosphatidylserine translocation from the inner to the outer leaflet of the plasma membrane at 32℃. Western blot assays analyzed heat-shock protein 70 (HSP 70), anti-apoptosis protein bcl-2 and pro-apoptosis protein bak expression of TGIV-infected cells at the above three temperatures. There are obvious expressions of HSP 70 and pro-apoptosis protein bak at 32℃. And anti-apoptosis protein bcl-2 was found expressing significantly at 25℃ only. Finally, fish were injected TGIV and held at 25 and 32℃. After 2 weeks, the cumulative mortality of virus-infected fish was 100% and 37.5% respectively. Besides, the 32℃ group showed more obvious TUNEL positive reaction than 25℃ group. The results revealed that hyperthermia-induced apoptsis might closely related with host defense mechanism against TGIV infection.

謝辭 i
中文摘要 ii
英文摘要 iv
目錄 vi
圖目錄 viii
前言 1
文獻整理 3
一、魚類虹彩病毒感染症之簡介 3
二、溫度與水產動物病毒致病之關連 6
三、水產動物病毒與細胞凋亡 10
四、細胞凋亡之簡介與分析法 13
五、細胞凋亡與熱休克蛋白 20
材料與方法 23
一、實驗材料 23
(一) 細胞株 23
(二) 台灣石斑虹彩病毒 24
(三) 實驗用魚 25
(四) 感染實驗用之循環控溫養殖系統 25
二、研究方法 26
(一) 不同溫度對石斑虹彩病毒增殖力影響 26
1、PCR測定 26
2、RV之收集 27
3、CAV之收集 27
(二) 不同溫度下石斑虹彩病毒在SB細胞株中凋亡之觀察 27
1、以流式細胞儀分析細胞週期及凋亡 27
(1) PI染色 27
(2) Annexin-Ⅴ染色 28
2、Annexin-Ⅴ螢光染色 28
3、Western blot偵測熱休克蛋白70、Bcl-2與Bak蛋白表現 28
(三) 不同溫度下臺灣石斑虹彩病毒感染對魚體中組織所誘發凋亡情形 30
1、虹彩病毒感染石斑魚之組織病理觀察 31
2、虹彩病毒誘發石斑魚細胞凋亡分析 32
結果 33
一、不同溫度對石斑虹彩病毒增殖力影響 33
(一) PCR測定 33
(二) 病毒增殖曲線圖 33
二、不同溫度下石斑虹彩病毒在SB細胞株中凋亡之觀察 34
(一)、以流式細胞儀分析細胞週期及凋亡細胞 34
1. PI染色觀察細胞週期變化 34
2. Annexin-Ⅴ與PI染色觀察凋亡細胞產生之情形 34
(二)、Annexin-Ⅴ與PI螢光染色觀察凋亡細胞與壞死細胞 35
(三)、Western blot偵測熱休克蛋白70、Bcl-2與Bak蛋白表現 36
1. 熱休克蛋白70表現情形 36
2. Bcl-2蛋白質表現情形 36
3. Bak蛋白質表現情形 36
三、不同溫度下臺灣石斑虹彩病毒於魚體中組織所誘發凋亡情形 37
(一)、利用H & E染色觀察虹彩病毒感染石斑魚之組織病理切片 37
(二)、利用TUNEL染色分析虹彩病毒誘發石斑魚細胞凋亡反應 37
討論 39
參考文獻 44
圖 62
表 89

圖　目　錄

圖一、不同溫度下病毒增殖PCR結果 62
圖二、各種不同溫度下病毒增殖曲線測定 63
圖三、以流式細胞儀分析18℃感染細胞之細胞週期 64
圖四、以流式細胞儀分析25℃感染細胞之細胞週期 65
圖五、以流式細胞儀分析32℃感染細胞之細胞週期 66
圖六、以流式細胞儀分析細胞週期之量化結果 67
圖七、以流式細胞儀分析18℃感染細胞之凋亡情形 68
圖八、以流式細胞儀分析25℃感染細胞之凋亡情形 69
圖九、以流式細胞儀分析32℃感染細胞之凋亡情形 70
圖十、以流式細胞儀分析凋亡細胞之量化結果 71
圖十一、以流式細胞儀分析壞死細胞之量化結果 72
圖十二、以螢光顯微鏡觀察凋亡與壞死細胞產生之情形 73
圖十三、以Western blot偵測25℃與32℃熱休克蛋白70表現情形 74
圖十四、以Western blot偵測25℃與32℃抗凋亡蛋白Bcl-2表現情形 75
圖十五、以Western blot偵測25℃與32℃促凋亡蛋白Bak表現情形 76
圖十六、溫度因子對病毒致病力影響 77
圖十七、25℃下罹病魚之組織病理切片H & E染色全圖 78
圖十八、25℃下罹病魚之組織病理切片各臟器H & E染色圖 79
圖十九、25℃下罹病魚之組織病理切片凋亡反應TUNEL染色全圖 80
圖二十、25℃下罹病魚之組織病理切片各臟器凋亡反應TUNEL染色圖 81
圖二十一、32℃下罹病魚之組織病理切片H & E染色全圖 82
圖二十二、32℃下罹病魚之組織病理切片各臟器H & E染色圖 83
圖二十三、32℃下罹病魚之組織病理切片凋亡反應TUNEL染色全圖 84
圖二十四、32℃下罹病魚之組織病理切片各臟器凋亡反應TUNEL染色圖 85
圖二十五、不同溫度各臟器細胞凋亡TUNEL陽性反應數量統計表 86

曾慷、何建國、翁少萍、黃志堅、侯少挺、羅均任、黃佛平、陳炯�� (1999) 。傳染性脾臟壞死病毒 (ISKNV) 感染途徑，寄主範圍及溫度敏感性的研究。中國病毒學。(14)：353-357。
周信佑、徐崇哲、陳莉菁 (2000)。石斑虹彩病毒病原性與傳播之研究。農委會漁業署養殖特刊第一號•魚病研究專集 (二十) 抽印本：61-70。
陳莉菁 (2000)。石斑虹彩病毒之病原性與疫苗開發之研究。國立台灣海洋大學水產養殖學系碩士學位論文。
瞿中和, 王喜中, 丁明孝 (2001)。 細胞分子生物學，九州圖書文物有限公司。519-521
陳慧如 (2002)。石斑魚泳鰾細胞株之建立與其在石斑魚虹彩病毒研究之應用。國立台灣海洋大學水產養殖學系碩士學位論文。
趙嘉本 (2003)。石斑魚虹彩病毒感染症。中華民國水產種苗協會月刊 (編)。65(9)。
林奕廷 (2004)。溫度因子對石斑魚虹彩病毒致病機制之影響：細胞凋亡扮演的角色。國立台灣海洋大學水產養殖學系碩士學位論文。
劉興懿 (2006)。台灣石斑虹彩病毒誘導石斑魚泳鰾細胞發生溫度相關凋亡之研究。國立台灣海洋大學水產養殖學系碩士學位論文。
Afford, S. and S. Randhawa (2000). Demystified... Apoptosis. Journal of Clinical Pathology, 53: 55–63.
Adams, J. M., and Cory S. (1998). The Bcl-2 protein family: arbiters of cell survival. Science, 281(5381): 1322-1326
Ahne, W. (1994). Viral infections of aquatic animals with special reference to Asian aquaculture. Annual Review of Fish Diseases, 4: 375-426.
Ahne, W., H. V. Bjorklund, S. Essbauer, N. Fijan, G. Kurath and J. R. Winton (2002). Spring viremia of carp. Diseases of Aquatic Organisms, 52: 261-272.
Alnemri, E. S., Livingston, D. J., Nichoson, D. W., Salvesen, G., Thomberry, N. A., Wong, W. W., and Yuan, J. (1996). Human ICE/CED-3 protease nomenclature. Cell, 87: 171.
Anders, K., W. Ahne and E. Kurstak (1989). A herpesvirus associated with an epizootic of epidermal papillomatosis in European smelt (Osmerus eperlunus). Viruses of Lower-Vertebrates, 184-97.
Anne, P. R. (1999). Preoperative paclitaxel, carboplatin, 5-FU and radiation in patients with resectable esophageal or gastric cancer: A phase I study. 41st Annual Meeting of the American Society for Therapeutic Radiology and Oncology, San Antonio, TX (USA)
Antonsson, B. (2001). Bax and other pro-apoptotic Bcl-2 family "killer-proteins" and their victim the mitochondrion. Cell and Tissue Research, 306: 347-61.
Arends, M. J., R. G. Moris and A. H. Wyllie (1990). Apoptosis the role of endonuclease. Journal of Pathology, 136: 593-608.
Arya, R. and S. C. Lakhotia (2008). Hsp60D is essential for caspase-mediated induced apoptosis in Drosophila melanogaster. Cell Stress and Chaperones, 13(4): 509-526.
Ashkenazi, A. and V. M. Dixit (1998). Death receptors: signaling and modulation. Science, 281(5381): 1305-1308.
Aubert, M., L. E. Pomeranz and J. A. Blaho (2007). Herpes simplex virus blocks apoptosis by precluding mitochondrial cytochrome c release independent of caspase activation in infected human epithelial cells. Apoptosis, 12: 19–35.
Behl, C. (2000). Apoptosis and Alzheimer's disease. Journal of Neural Transmission, 107: 1325-1344.
Bettaieb, A. and D. A. Averill (2005). Thermotolerance induced at a mild temperature of 40 degrees C protects cells against heat shock-induced apoptosis. Journal of cellular physiology, 205(1): 47-57.
Bjoerklund, H. V., T. R. Johansson and A. Rinne (1997). Rhabdovirus-induced apoptosis in a fish cell line is inhibited by a human endogenous acid cysteine proteinase inhibitor. Journal of Virology, 71, 5658-5662.
Cervos-Navarro, J. and T. E. O. Schubert (1996). Pitfalls in the evaluation of apoptosis using TUNEL. Brain pathology, 6:347-348.
Chao, D. T. and S. J. Korsmeyer (1998). Bcl-2 family: regulators of cell death. Annual Review of Immunology, 16: 395-419.
Chao, C. B., C. Y. Chen, Y. Y. Lai, C. S. Lin and H. T. Huang (2004). Histological, ultrastructural, and in situ hybridization study on enlarged cells in grouper Epinephelus hybrids infected by grouper iridovirus in Taiwan (TGIV). Diseases of Aquatic Organisms, 58: 127-142.
Chen, S. P., J. L. Wu, Y. C. Su and J. R. Hong (2007). Anti-Bcl-2 family members, zfBcl-xL and zfMcl-1a, prevent cytochrome c release from cells undergoing betanodavirus-induced secondary necrotic cell death. Apoptosis. 12: 1043-1060
Chi, S. C., S. C. Lin, H. M. Su and W. W. Hu (1999). Temperature effect on nervous necrosis virus infection in grouper cell line and in grouper larvae. Virus Research, 63: 107-114.
Chi, S. C., J. R. Shieh and S. J. Lin (2003). Genetic and antigenic analysis of betanodaviruses isolated from aquatic organisms in Taiwan. Diseases of Aquatic Organisms, 55: 221-228.
Chinchar, V.G., S. Essbauer, J. G. He, A. Hyatt, T. Miyazaki, V. Seligy, T. Williams (2005). Part II The Double Stranded DNA Viruses, Family Iridoviridae. VIIIth Report of the International Committee on Taxonomy of Viruses, 145–162.
Chiou, P. P., C. H. Kim, P. Ormonde and J. A. Leong (2000). Infectious hematopoietic necrosis virus matrix protein inhibits host-directed gene expression and induces morphological changes of apoptosis in cell cultures. Journal of Virology, 74: 7619-7627.
Chou, H. Y., C. C. Hsu and T. Y. Peng (1998). Isolation and characterization of a pathogenic iridovirus from cultured grouper (Epinephelus s.) in Taiwan. Fish Pathology., 33: 201-206.
Cotman, C. W., E. R. Whittemore, J. A. Watt, A. J. Anderson and D. T. Loo (1994). Possible role of apoptosis in Alzheimer's disease. Annals of the New York Academy of Sciences, 747: 36-49.
Cotman, C. W. and A. J. Anderson (1995). A potential role for apoptosis in neurodegeneration and Alzheimer's disease. Molecular Neurobiology, 10: 19-45.
Dorson, M. and C. Torchy (1981). The influence of fish age and water temperature on mortalities of rainbow trout, Salmo gairdneri Richardson, caused by a European strain of infectious pancreatic necrosis virus. Journal of Fish Diseases, 3: 213-221.
Eick, D. and H. Hermeking (1996). Viruses as pacemakers in the evolution of defence mechanisms against cancer. Trends in Genetics, 12: 4-6.
Eleouet, J. F., N. Druesne, S. Chilmonczyk, D. Monge and B. Delmas (2001). Comparative study of in-situ cell death induced by the viruse of viral haemorrhagic septicaemia (VHS) and infection pancreatic necrosis (IPN) in rainbow trout. Journal of Comparative Pathology, 124: 300-307.
Essbauer, S. and W. Ahne (2002). The epizootic haematopoietic necrosis virus (Iridoviridae) induces apoptosis in vitro. Journal of Veterinary Medical Education, 49: 25-30.
Farrow, S. N., J. H. M. White, I. Martinou, T. Raven, K. T. Pun, C. J. Grinham, J. C. Martinou and R. Brown (1995). Cloning of a Bcl–2 homologue by interaction with adenovirus EIB19K. Nature, 374: 731-736.
Francoise, L. M., G. Christiane, L. Philippe, R. Catherine, L. Sylvie, B. Elisabeth and N. Adrien (1998). TUNEL apoptosis cell detection in tissue sections: critical evaluation and improvement. The Journal of Histochemistry and cytochemistry, 46(3): 327-334.
Franceschi, C. (1989). Cell proliferation, cell death and aging. Aging (Milano), 1(1): 3-5.
Fry, F. G. J. (1967). Responses of vertebrate poikilotherms to temperature. In “Thermobilology” (ed. by A. H Rose) N. Y. Academic, London, pp. 375-409.
Fukuda, Y., H. D. Nguyen, M. Furuhashi and T. Nakai (1996). Mass mortality of cultured sevenband grouper, Epinephelus septemfasciatus, associated with viral nervous necrosis. Fish Pathology, 31: 165-170.
Gilad, O., S. Yun, M. A. Adkison, K. Way, N. H. Willits, H. Bercovier and R. P. Hedrick (2003). Molecular comparison of isolates of an emerging fish pathogen, koi herpesvirus, and the effect of water temperature on mortality of experimentally infected koi. Journal of General Virology, 83: 2661-2667.
Granja, C. B., L. F. Aranguren, O. M. Vidal, L. Aragon and M. Dalazar (2003). Does hyperthermia increase apoptosis in white spot syndrome virus (WSSV)-infected Litopenaeus vannamei? Diseases of Aquatic Organisms, 54: 73-78.
Granja C. B., O. M. Vidal, G. Parra, M. Salazar (2006). Hyperthermia reduces viral load of white spot syndrome virus in Penaeus vannamei. Diseases of Aquatic Organisms, 68: 175-180.
Grasl-Kraupp, B., B. Ruttkay-Nedecky, H. Koudelka, K. Bukowska, W. Bursch and R. Schulte-Hermann (1995). In situ detection of fragmented DNA (TUNEL assay) fails to discriminate among apoptosis, necrosis and autolytic cell death : a cautionary note. Hepatology, 21: 1465-1468.
Green, D. R. and J. C. Reed. (1998). Mitochondria and apoptosis. Aging, 1(1): 3-15.
Guo, Y. X., T. Wei, K. Dallmann and J. Kwang (2003). Induction of caspase-dependent apoptosis by betanodaviruses GGNNV and demonstration of protein alpha as an apoptosis inducer. Virology, 308: 74-82.
Harrington, E. A., M. R. Bennett, A. Fandini and G. I. Evan (1994). Modulation of myc-induced apoptosis by specific cytokines. The EMBO Journal, 13: 3286-3295.
He, J. G., S. P. Weng, K. Zeng, Z. J. Huang and S. M. Chan (2000). Systemic disease caused by an iridovirus-like agent in cultured mandarinfish, Siniperca chuatsi (Basilewsky), in China. Journal of Fish Diseases, 23: 219-222.
He, J. G., M. Deng, S. P. Weng, Z. Li, S. Y. Zhou, Q. X. Long, X. Z. Wang and S. M. Chan (2001). Complete genome analysis of the mandarin fish infectious spleen and kidney necrosis iridovirus. Virology, 291: 126-139.
He, J. G., K. Zeng, S. P. Weng and S. M. Chan (2002). Experimental transmission, pathogenicity and physical-chemical properties of infectious spleen and kidney necrosis virus (ISKNV). Aquaculture, 204: 11-24.
Hedrick, R. P., T. S. McDowell, J. M. Groff, S. Yun and W. H. Wingfield (1992). Isolation and some properties of an iridovirus-like agent from white sturgeon Acipenser transmontanus. Disease of Aquatic Organisms, 12: 75-81.
Hengartner, M. O. (2000). The biochemistry of apoptosis. Nature, 407: 770-776.
Henry, H., A. Thomas, Y. Shen and E. White (2002). Regulation of the mitochondrial checkpoint in p53-mediated apoptosis confers resistance to cell death. Oncogene, 21: 748-760.
Hetrick, F. M., J. L. Fryer and M. D. Knittel (1979). Effect of water temperature on the infection of rainbow trout Salmo gairdneri Richardson with infectious haematopoietic necrosis virus. Journal of Fish Disease, 2: 253-257.
Hong, J. R., T. L. Lin, Y. L. Hsu and J. L. Wu (1998). Apoptosis precedes necrosis of fish cell line with infectious pancreatic necrosis virus infection. Virology, 250: 76-84.
Hong, J. R., Y. L. Hsu and J. L. Wu (1999). Infectious pancreatic necrosis virus induces apoptosis due to down-regulation of survival factor MCL-1 protein expression in a fish cell line. Virus Research, 63: 75-83.
Hong, J. R., Y. L. Hsu, and J. L. Wu (1999). Infectious pancreatic necrosis virus induces apoptosis due to down-regulation of survival factor MCL-1 protein expression in a fish cell line. Virus Research., 63: 75-83.
Hong, J. R. and J. L. Wu (2002). Induction of apoptotic death in cells via Bad gene expression by infectious pancreatic necrosis virus infection. Cell Death Fiffer, 9: 113-124.
Hong, J. R., H. Gong and J. L. Wu (2002). IPNV VP5, a Novel Anti-apoptosis Gene of the Bcl-2 Family, Regulates Mcl-1 and Viral Protein Expression. Virology, 295: 217-229.
Hong, J. R. and J. L. Wu (2002). Induction of apoptotic death in cells via Bad gene expression by infectious pancreatic necrosis virus infection. Cell Death and Differentiation, 9: 113-124.
Hong, J. R., B. J. Guan, G. M. Her, O. Evensen, N. Santi and J. L. Wu (2008). Aquatic birnavirus infection activates the transcription factor NF-κB via tyrosine kinase signalling leading to cell death. Journal of Fish Disease, 31(6): 451-460.
Huang, D. C., and A. Strasser (2000). BH3-Only proteins-essential initiators of apoptotic cell death. Cell, 103: 839-42.
Huang, X., Y. Huang, J. Gong, Y. Yan and Q. Qin (2008). Identification and characterization of a putative lipopolysaccharide-induced TNF- alpha factor (LITAF) homolog from Singapore grouper iridovirus. Biochemical and Biophysical Research Communications, 373(1): 140-145.
Imajoh, M. and S. Suzuki (1999). Apoptosis induced by a marine birnavirus in established cell lines from fish. Fish Physiology and Biochemistry, 34, 73-79.
Imajoh, M., H. Sugiura and S. Oshima (2004). Morphological changes contribute to apoptotic cell death and are affected by caspase-3 and caspase-6 inhibitors during red sea bream iridovirus permissive replication. Virology, 322: 220-230.
Inouye, K., K. Yamano, Y. Maeno, K. Nakajima, M. Matsuoka, Y. Wada and M. Sorimachi (1992). Iridovirus infection of cultured red sea bream, Pagrus major. Fish Patholojy, 27: 19-27.
Iordanskiy, S., Y. Zhao, L. Dubrovsky, T. Iordanskaya, M. Chen, D. Liang and M. Bukrinsky (2004). Heat Shock Protein 70 Protects Cells from Cell Cycle Arrest and Apoptosis Induced by Human Immunodeficiency Virus Type 1 Viral Protein R. Journal of Virology, 9697–9704.
Ishizaki, Y. (1998). Physiological functions of programmed cell death. Seikagaku, 70: 365-370.
Ito, T., D. Xingming, C. Boyd and M. W. Stratford (1997). Bcl-2 phosphorylation required for anti-apoptosis function. The Journal of Biological Chemistry, 272: 11671-11673.
Iwama, G. K., M. M. Vijayan, C. Pereira and G. Kruzynski (1998). Sublethal Concentrations of Contaminant Induce the Expression of Hepatic Heat Shock Protein 70 in Two Salmonids. Aquatic Toxicology, 40: 101-108.
Jaattela, M. (1999). Heat shock proteins as cellular lifeguards. Annals of medicine, 31(4): 261-71.
Kerr, J. F., A. H. Wyllie and A. R. Currie (1972). Apoptosis: a basis biological phenomenon with wide-ranging implications in tissue kinetics. British Journal of Cancer, 26: 239-257.
Kerr, J. F. (1995). Neglected opportunities in apoptosis research. Trends in cell biology, 5: 55-57.
Langdon, J. S., J. D. Humphrey, L. D. Williams, A. D. Hyatt and H. A. Westbury (1986). First isolation from Australian fish: an iridovirus-like pathogen from redfin perch, Perca fluviatilis L. Journal of Fish Diseases, 9: 263-268.
LaPatra, S. E., J. M. Groff, G. R. Jones, B. Munn, T. L. Patterson, R. A. Holt, A. K. Hauck and R. P. Hedrick (1994). Occurrence of white sturgeon iridovirus infections among cultured white sturgeon in the Pacific Northwest. Aquaculture, 126: 201-210.
Lapierre, J., D. Larrivee and L. Berthiaume (1988). Influence of water temperature and fish age on mortality in brook trout (Salvelinus fontinalis) infected with infectious pancreatic necrosis virus (IPNV). Aquaculture, 59: 81-92.
Le Breton, A., L. Grisez, J. Sweetman and F. Ollevier (1997). Viral nervous necrosis (VNN) associated with mass mortalities in cage-reared sea bass, Dicentrarchus labrax (L.). Journal of Fish Diseases, 20: 145-151.
Leu, J. H., Y. C. Kuo, G. H. Kuo and C. F. Lo (2008). Molecular cloning and characterization of an inhibitor of apoptosis protein (IAP) from the tiger shrimp, Penaeus Monodom. Developmental and Comparative Immunology, 32: 121-133.
Li G. C. and A. Laszlo, (1985). In Atchinson B. J. and Walden D. B. (eds), Changes in Eukaryotic Gene Expression in Response to Environmental Stress. Academic Press, New York, 227.
Lin, P. W., Y. J. Huang, J. A. C. John, Y. N. Chang, C. H. Yuan, W. Y. Chen, C. H. Yeh, S. T. Shen, F. P. Lin, W. H. Tsui and C. Y. Chang (2008). Iridovirus Bcl-2 protein inhibits apoptosis in the early stage of viral infection. Apoptosis, 13: 165-176.
Lindquist S. and E. A. Craig (1988) The Heat-shock Proteins. Annual Review of Genetics. 22: 631-677.
Lockshin, R. A. and J. Beaulaton (1974). Programmed cell death. Life Science. 15: 1549-65.
Marshall, W. L., C. Yim, E. Gustafson, T. Graf, D. R. Sage, K. Hanify, L. Williams, J. Fingeroth and R. W. Finberg (1999). Epstein-Barr virus encodes a novel homolog of the bcl-2 oncogene that inhibits apoptosis and associates with Bax and Bak. Journal of Virology, 73: 5181-5185.
Maroto, R and J. R. Perez-Polo (1997). Bcl-2-related protein expression in apoptosis: oxidative stress versus serum deprivation in PC12 cells. Journal of Neurochemistry , 69: 514-23.
Miyata, M., K. Matsuno, S. J. Jung, Y. Danayadol and T. Miyazaki (1997). Genetic similarity of iridoviruses from Japan and Thailand. Journal of Fish Diseases, 20, 127-134.
Mori, K., T. Nakai, M. Nagahara, K. Muroga, T. Mekuchi and T. Kanno (1991). A viral disease in hatchery-reared larvae and juveniles of redspotted grouper. Gyobyo Kenkyu. 26: 209-210.
Morimoto, R. I., A. Tissieres and C. Georgopoulos (1990). Stress Proteins in Biology and Medicine. Cold Spring Harbor Laboratory Press, 19.
Mosser, J., N. Andrieux, P. Fergelot, I. Gicquel, V. Lelaure, F. Galibert and V. David (1997). A 1200-kilobase transcription map encompassing the D6S105 locus at 6p21.3. Genomics, 46(3): 487-490.
Nakajima, K. and M. Sorimachi (1994). Biological and physico-chemical properties of the iridovirus isolated from cultured red sea bream, Pagrus major. Fish Pathology, 29: 29-33.
Neukirch, M. (1992). VHSV-infected rainbow trout, Oncorhynchus mykiss -- a model for the study of temperature dependant excretion of viral agents. Diseases in Asian Aquaculture.1.Proceedings of the First Symposium on Diseases in Asian Aquaculture, 26-29 November 1990, Bali, Indonesia. Manila, 273-280
Nover, L. (1991). Heat Shock Response. CRC Press: Boca Raton.
O'Brien, V. (1998). Viruses and apoptosis. The Journal of General Virology, 79: 1833-1845.
Okamoto, N., R. Yasutomi, H. Shibazaki, S. Hanzawa and T. Sano (1987). The influence of immersing temperature for inoculation with IPNV and/or rearing temperature on mortality of rainbow trout fry postinfection. Bulletin of the Japanese Society of Scientific Fisherie, 53: 1125-1128
Oseko, N., M. Yoshimizu and T. Kimura (1988). Effect of water temperature on artificial infection of Rhabdovirus olivaceus (hirame rhabdovirus; HRV) to hirame (Japanese flounder, Paralichtys olivaceus). Fish Pathology, 23, 125-132.
Perng, G; S. M. Slanina, A. Yukht, H. Ghiasi,; A. B. Nesburn and S. L. Wechsler (2000). The latency-associated transcript gene enhances establishment of herpes simplex virus type Ⅰ latency in rabbits. Journal of Virology, 74(4): 1885-1891.
Perry, G., A. Nunomura, P. Lucassen, H. Lassmann and M. A. Smith (1998). Apoptosis and Alzheimer's disease. Science, 282: 1268-1269.
Postigo, A., J. R. Cross, J. Downward and M. Way (2006). Interaction of F1L with the BH3 domain of Bak is responsible for inhibiting vaccinia-induced apoptosis. Cell Death Differ, 13: 1651–1662.
Qin, Q. W., T. J. Lan, Y. M. Sin, H. Shen, S. F. Chang, G. H. Ngoh and C. L. Chen (2001). Electron microscopic observations of a marine fish iridovirus isolated from brown-spotted grouper, Epinephelus tauvina. Journal of Virological Methods. 98(1): 17-24.
Qin, Q. W., C. Shi, K. Y. Gin and T. J. Lam (2002). Antigenic characterization of a marine fish iridovirus isolated from grouper, Epinephelus spp. Journal of Virological Methid, 106(1): 89-96.
Qu, S., X. Zhang, C. Zheng, G. Hu, T. Ai, G. Ding, Y. Yu and Y. Liu (2000). Apoptosis of the cell line from grass carp (CIK) induced by fish reovirus. Acta Hydrobilogica Sinica, 24: 616-621.
Reed, J. C. (1999). Mechanisms of apoptosis avoidance in cancer. Current Opinion in Oncology, 11: 68-75.
Richa, A., S. C. Lakhotia (2008). Hsp60D is essential for caspase-mediated induced apoptosis in Drosophila melanogaster. Cell stress and chaperones, 13(4): 509-526.
Rijiravanich, A., C. L. Browdy and B. Withyachumnarnkul (2008). Knocking down caspase-3 by RNAi reduces mortality in Pacific white shrimp Penaeus (Litopenaeus) vannamei challenged with a low dose of white-spot syndrome virus. Fish and Shellfish Immunology, 24: 308-313.
Rodenhiser, D., J. H. Jung, and B. G. Atkinson (1985). Mammalian lymphocytes: stress-induced synthesis of heat shock proteins in vitro and vivo. Can. J. Biochem. Cell Biology. 63:711-722.
Sanchez Y., J. Taulien, K. A. Borkovich and S. Lindquist (1992) Hsp104 is required for tolerance to many forms of stress. The EMBO Joutnal. 11(6): 2357-2364.
Sanders B. (1990) Stress proteins: Potential as Multitiered Biomarkers. In J. F. McCarthy and L. R. Shugart, eds., Biomarkers of Environmental
Contamination. Lewis, Boca Raton, FL, 165-1991.
Santos, H. B., R. G. Thome, F. P. Arantes, Y. Sato, N. Bazzoli and E Rizzo (2008). Ovarian follicular atresia is mediated by heterophagy, autophagy, and apoptosis in Prochilodus argenteus and Leporinus taeniatus (Teleostei: Characiformes). Theriogenology, 70(9): 1449-1460.
Sasano, H. (1995). In situ end labeling and its applications to the study of endocrine disease: how can we study programmed cell death at a molecular level. Seminats in Arthritis and Rheumatism, 32: 345-369.
Schultz, D. R. and W. J. Harrington (2003). Apoptosis: programmed cell death at a molecular level. Seminars in Arthritis and Rheumatism, 32: 345-369.
Shi, C. Y., Y. G. Wang, S. L. Yang, J. Huang and Q.Y. Wang (2004). The first report of an iridovirus-like agent infection in farmed turbot, Scophthalmus maximus, in China. Aquaculture, 236: 11-25.
Simonian, P. L., D. A. M. Grillot and G. Nunez (1997). Bcl–2 and BCl–xL can differentially block chemotherapy-induced ceel death. Blood, 90: 1208-1216.
Stadelmann, C. and H. Lassmann (2000). Dection of apoptosis in tissue sections. Cell and Tissue Research, 301: 19-31.
Sudthongkong, C., M. Miyata and T. Miyazaki (2002). Iridovirus disease in two ornamental tropical freshwater fishes: African lampeye and dwarf gourami. Disease of Aquatic Organisms, 48: 163-173.
Sudthongkong, C., M. Miyata and T. Miyazaki (2002). Viral DNA sequences of genes encoding the ATPase and the major capsid protein of tropical iridovirus isolates which are pathogenic to fishes in Japan, South China Sea and Southeast Asian countries. Archives of Virology, 147: 2089-2109.
Susin, S. A., N. Zamzami, M. Castedo, T. Hirsch, P. Marchetti, A. Macho, E. Daugas, M. Geuskens and G. Kroemer (1996). Bcl-2 inhibits the mitochondrial release of an apoptogenic protease. Journal of Experimental Medicine, 184: 1331-1341.
Susin, S. A., N. Zamzami, M. Castedo, E. Daugas, H. G. Wang, S. Geley, F. Fassy, J. C. Reed and G. Kroemer (1997). The central executioner of apoptosis: multiple connections between protease activation and mitochondria in Fas/APO-1/CD95 and ceramide-induced apoptosis. The Journal of Experimental Medicine, 186: 25-37.
Wang, L., B. Zhi, Wenlin Wu and X. Zhang (2008). Requirement for shrimp caspase in apoptosis against virus infection. Developmental and comparative immunology, 32(6): 706-715.
Wang, W and X, Zhang (2008). Comparison of antiviral efficiency of immune responses in shrimp. Fish and Shellfish Immunology, 25(5): 522-527.
Watson, L. R., J. M. Groff and R. P. Hedrick (1998). Replication and pathogenesis of white sturgeon iridovirus (WSIV) in experimentally infected white sturgeon, Acipenser transmontanus, juveniles and sturgeon cell lines. Diseases of Aquatic Organisms, 32: 173-184.
Weber, L. P. and D. M. Janz (2001). Effect of beta -naphthoflavone and dimethylbenz[a]anthracene on apoptosis and HSP70 expression in juvenile channel catfish (Ictalurus punctatus) ovary. Aquatic Toxicology, 54(1-2): 39-50.
White, E. (1994). Tumor biology-p53, guardian of Rb. Nature 371: 21-22.
Whittington, R. J. and G. L. Reddacliff (1995). Influence of environmental temperature on experimental infection of redfin perch (Perca fluviatilis) and rainbow trout (Oncorhynchus mykiss) with epizootic haematopoietic necrosis virus, an Australian iridovirus. Australian veterinary journal, 72: 421-424.
Williams, T. (1985). Current topics in microbiology and Immunology. Iridoviridae, 116: 1-173.
Williams, T. (1996). The iridoviruses. Advances in virus research, 46: 345-412.
Williams, T., G. Chinchar, G. Darai, A. Hyatt, J. Kalmakoff and V. Seligy (2000). Iridoviridae. In: Van Regenmortel. Virus Taxonomy of Viruses, pp: 167-182.
Wolf, K. (1988). Fish viruses and fish viral diseases. Ithaca, New York: Cornell University Press.
Wu, H. C., C. S. Chiu, J. L. Wu, H. Y. Gong, M. C. Chen, M. W. Lu and J. R. Hong (2008). Zebrafish anti-apoptotic protein zfBcl-xL can block betanodavirus protein α-induced mitochondria-mediated secondary necrosis cell death. Fish and Shellfish Immunology, 24(4): 436-449.
Yasuda, M., S. Unemura, Y. Osamura, T. Kenjo, Y. Tsutsumi (1995). Apoptotic cells in the human endomtrium and placental villi: pitfalls in applying the TUNEL method. Archives of Histology and Cytology, 58: 185-190.
Yokoyama, S., S. Koshio, N. Takakura, K. Oshida, M. Ishikawa, F. J. Gallardo-Cigarroa, M. R. Catacutan and S. Teshima (2006). Effect of dietary bovine lactoferrin on growth response, tolerance to air exposure and low salinity stress conditions in orange spotted grouper Epinephelus coioides. Aquaculture, 255: 507-513.
Zakeri, Z. and R. A. Lockshin (1994). Physiological cell death during development and its relationship to aging. Annals of the New York Academy of Science, 719: 212-29.