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研究生:黃奕絲
研究生(外文):Yi-Sih Huang
論文名稱:點帶石斑魚神經壞死病毒外鞘蛋白基因選殖與表現
論文名稱(外文):Molecular cloning and expression of a grouper(Epinephelus coioides) nervous necrosis virus coat protein gene
指導教授:李國誥李國誥引用關係
指導教授(外文):Kuo-Kau Lee
學位類別:碩士
校院名稱:國立臺灣海洋大學
系所名稱:水產養殖學系
學門:農業科學學門
學類:漁業學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:86
中文關鍵詞:基因選殖表現神經壞死病毒外鞘蛋白基因
外文關鍵詞:molecular cloningexpressionnervous necrosis virus coat protein gene
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病毒性神經壞死症(Viral nervous necrosis disease, VNN disease),嚴重衝擊石斑魚養殖產業發展並造成重大的經濟損失,其病原為神經壞死病毒主要感染魚苗及幼魚,並引起高達 80-100%的死亡率。因此,疾病的預防與控制遂成為一重要課題,除了養殖環境的改善、種魚篩選及優質種苗生產外,疫苗的開發成為有效控制疾病的策略之ㄧ。本研究以大腸桿菌系統來表現石斑魚神經壞死病毒的外鞘蛋白基因,利用基因重組方式生產該病毒的外鞘蛋白,期望能作為製成有效次單位疫苗的應用。
本研究從石斑魚神經壞死病毒中選殖之外鞘蛋白基因長度為 1017 bp,並在 5’ 端及 3’ 端分別設計 Hind? 切位及 XhoΙ 切位,經定序比對與石斑魚 Epinephelus coioides nervous necrosis virus truncated coat protein gene 相似度高達 99%。將此基因片段與 pET-32a (+)(Novagen)表現載體進行接合後,轉型至 E. coli(BL21(DE3)pLysS),以完成構築。
將構築完成的菌株,於 LB/Ampcillin broth, 37℃,培養至 OD600=0.6,加入濃度為 1 mM IPTG 進行誘導,分別經不同時間點誘導後,收集總蛋白、可溶性蛋白及不可溶性蛋白,以 Sodium dodecyl sulfate-polyacrylamide gel 電泳分析,結果發現在 IPTG 誘導 2 小時其外鞘蛋白表現量最高,並且會以包涵體之形式存在,分子量大小為37 kDa。另外以同樣培養方式於不同濃度的 IPTG 培養液培養,經 2小時誘導後,發現表現蛋白在 0.8 mM IPTG 誘導 2 小時其表現量最高,因此可以在此條件下以鎳金屬螯合層析法進行純化,本研究結果可做為未來次單位疫苗開發及疾病檢測之應用。
Viral nervous necrosis disease may cause serious economic threat to grouper culture industry. The disease may cause high mortality(80-100%)particularly in larva and juvenile. Therefore, the control and prevention of this disease is very important. Except improving culture conditions, screening broodstock fish or raising healthy fry. Using vaccine is an effective method to control the disease. The present study used E coli system to express grouper nervous necrosis virus coat protein gene for further production of recombinant coat protein as a possible candidate for efficient prophylactic subunit vaccine development.
The coat protein gene was cloned from grouper nervous necrosis virus with a fragment size of 1017 bp. The 5’ end and 3’ end were designed as Hind? site and XhoΙ site, respectively. The nucleotide sequence of the cloned gene were compared with Epinephelus coioides nervous necrosis virus truncated coat protein gene and exhibited 99% similarity. The gene of coat protein was cloned into a pET-32a (+) expression vector and expressed in E coli (BL21 (DE3) pLysS).
The recombinant E coli was cultured in LB/Ampcillin broth at 37℃. When the bacteria grown to OD600=0.6, 1 mM IPTG was added to induce its expression. Total protein, soluble protein and insoluble protein were collected at various time, then sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used for identification. The result revealed that the use of IPTG to induce 2 hours exhibited high coat protein expression with the formation of inclusion body. The coat protein expressed was a 37 kDa protein as measured by SDS-PAGE. Furthermore, the use of 0.8 mM IPTG to induce 2 hours exhibited high coat protein expression. Metal chelate chromatography was found to be useful for recombinant protein purification. The results revealed that the 37 kDa recombinant protein could be used as a candidate for future development of subunit vaccine and application of disease diagnosis.
謝辭……………………………………………………………………....i
中文摘要…………………………………………………………………iii
英文摘要………………………………………………………………....v
目錄………………………………………………………………………vii
圖目錄…………………………………………………………………….?
一、 前言……………………………………………………....………....1
二、 文獻整理………………………………………………………..…. 3
三、 材料方法…………………………………………………………...22
3.1石斑魚神經壞死病毒增殖及確認……………………………..….22
3.1.1石斑魚神經壞死病毒來源……………………………………..22
3.1.2石斑魚神經壞死病毒增殖……………………………………..22
3.1.2.1 石斑魚腦細胞(GBC1)之培養…………………………..22
3.1.2.2 石斑魚腦細胞(GBC1)的冷凍保存……………………..22
3.1.2.3 石斑魚腦細胞(GBC1)的解凍…………………….........23
3.1.2.4 石斑魚神經壞死病毒的增殖……………………................23
3.1.3 石斑魚神經壞死病毒的確認…………………………………..23
3.1.3.1 病毒核酸進行RT-PCR及Nested PCR確認……………..23
3.1.3.1.1 NNV RNA 的萃取……………………...........................23
3.1.3.1.2 反轉錄-聚合?連鎖反應…………………….................24
3.1.3.1.3 巢式聚合?連鎖反應(nested-PCR)………………...25
3.1.3.2 病毒核酸定序………………...............................................25
3.1.3.2.1 膠體純析(Gel elution)..……………………….……..25
3.1.3.2.2 定序載體的構築(Construction)與選殖(Selection)
………………….…………………………………………………26
3.1.3.2.3 接合作用(Ligation)..……………………….………26
3.1.3.2.4 轉型作用(Transformation)..………………………..26
3.1.3.2.5 篩選(Selection) ..…………………………………..27
3.1.3.2.6 重組質體 DNA 的萃取(Plasmid extraction)..……27
3.1.3.2.7 限制?酵素切割(Restriction enzyme digestion)..…28
3.1.3.2.8 定序及序列比對..…….………………………………..28
3.1.3.3 病毒顆粒層次的確認..……………………………………..28
3.1.3.3.1 穿透式電子顯微鏡負染色觀察……………………….28
3.2石斑魚神經壞死病毒外鞘蛋白基因選殖..……………………….28
3.2.1 石斑魚神經壞死病毒外鞘蛋白基因專一性引子之設計…….28
3.2.2 石斑魚神經壞死病毒外鞘蛋白基因片段序列選殖…….........29
3.2.3 石斑魚神經壞死病毒外鞘蛋白基因片段定序…….................29
3.3 石斑魚神經壞死病毒外鞘蛋白基因表現載體的構築……..........29
3.3.1 勝任細胞的製備…….................................................................29
3.3.2 石斑魚神經壞死病毒外鞘蛋白基因表現載體的構築…….....30
3.4 石斑魚神經壞死病毒重組外鞘蛋白的表現與純化……..............30
3.4.1 石斑魚神經壞死病毒重組外鞘蛋白的誘導表現……..............31
3.4.1.1 細胞萃取液(cell extract)製備...........................................31
3.4.1.2 可溶性蛋白與不可溶性蛋白的收集…….............................31
3.4.1.3 蛋白質膠體電泳及染色…….................................................31
3.4.1.4 NCP最佳的誘導條件測試….................................................32
3.4.2以Ni2+-NTA親合性管柱純化石斑魚神經壞死病毒重組外鞘蛋白…...............................................................................................33
3.4.2.1鎳金屬螯合層析法(metal chelate chromatography).........33
四、 結果…………………………………………………………………34
五、 討論…………………………………………………………………37
六、 參考文獻……………………………………………………………42
圖…………………………………………………………………………57
附錄……………………………………………………………………....70
林式修,2003。魚類免疫與疫苗。水產專訊,3:14-18。
林清龍,2004。石斑魚養殖要點。養殖漁業經營管理手冊技術篇,漁業署。
邵冠榮,2006。台灣沿海野生魚種帶原結病毒的田野調查與分析。
國立台灣大學動物學研究所,碩士論文。
陳政忻,2005。動物疫苗市場現況。農業生技產業季刊,第二期,35-42頁。
蓋玉軒,2005。石斑魚神經壞死症病毒去活化疫苗的製備與效能評估。國立台灣大學動物學研究所,碩士論文。
齊肖琪,2006。魚類神經壞死症病毒快速檢測試劑。水產種苗(FBA)。第98 期,1-5 頁。
漁業年報,2007。中華民國台灣地區漁業年報 2007 年版,行政院農委會。
劉翠玲,2007。水產養殖科技及產業的推進器-專訪水產種苗協會動物生技,第十期,71-74頁。
蘇惠美,2003。石斑魚繁養殖及病害防治講習班。水試專訊,4: 25-29。
蘇遠志,2003。後基因體時代之生物技術,教育部顧問室。
Arimoto, M., Mushiake, K., Mizuta, Y., Nakai, T., Muroga, K., Furusawa, I., 1992. Detection of striped jack nervous necrosis virus (SJNNV) by enzyme-linked immunosorbent assay (ELISA). Fish Pathology 27, 191-195.
Arimoto, M., Sato, J., Maruyama, K., Mimura, G., Furusawa, I., 1996. Effect of chemical and physical treatments on the inactivation of striped jack nervous necrosis virus (SJNNV). Aquaculture 143, 15-22.
Arthur, J.R., Ogawa, K., 1996. A brief overview of disease problems in the culture of marine finfishes in east and Southeast Asia. In: Main, K.L., Rosenfeld, C. (Eds.), Aquaculture Health Management Strategies for Marine Fishes. The Oceanic Institute, Makapu'u Point, HI, USA, pp. 9-31.
Aspehaug, V., Devold, M., Nylund, A., 1999. The phylogenetic relationship of nervous necrosis virus from halibut (Hippoglossus hippoglossus). Bulletin of the European Association of Fish Pathologists 19, 196-202.
Benoit, R.F., Matlin, N.A., 1966. Control of Saprolegnia on eggs of
rainbow trout (Salmo gairdneri) with ozone. Transactions of the American Fisheries Society 95, 430-432.
Bloch, B., Gravningen, K., Larsen, J.L., 1991. Encephalomyelitis among turbot associated with a picornavirus-like agent. Diseases of Aquatic Organisms 10, 65-70.
Boonyaratpalin, S., Supamattaya, K., Kasornchandra, J., Hoffmann, R.W., 1996. Picorna-like virus associated with mortality and a spongious encephalopathy in grouper Epinephelus malabaricus. Diseases of Aquatic Organisms 26, 75-80.
Breuil, G., Bonami, J.R., Pepin, J.F., Pichot, Y., 1991. Viral infection (picorna-like virus) associated with mass mortalities in hatchery-reared sea-bass (Dicentrarchus labrax) larvae and juveniles. Aquaculture 97, 109-116.
Breuil, G., Romestand, B., 1999. A rapid ELISA method for detecting specific antibody level against nodavirus in the serum of the sea bass, Dicentrarchus labrax (L.): application to the screening of spawners in a sea bass hatchery. Journal of Fish Diseases 22, 45-52.
Buchan, K.A.H., Martin-Robichaud, D.J., Benfey, T.J., MacKinnon, A-M., Boston, L., 2006. The efficacy of ozonated seawater for surface disinfection of haddock (Melanogrammus aeglefinus) eggs against piscine nodavirus.Aquacultural Engineering 35, 102-107.
Chang, S.F., Ngoh, G.H., Kueh, S., 1997. Detection of viral nervous necrosis nodavirus by reverse transcription polymerase chain reaction in locally farmed marine food fish. Singapore Veterinary Journal 21, 39-44.
Chang, S.F., Ngoh, G.H., Kueh, L.F.S., Qin, Q.W., Chen, C.L., Lam, T.J., Sin, Y.M., 2001. Development of a tropical marine fish cell line from Asian seabass (Lates calcarifer) for virus isolation. Aquaculture 192, 133-145.
Chi, S.C., Hu, W.W., Lo, B.L., 1999. Establishment and characterization of a continuous cell line (GF-1) derived from grouper, Epinephelus coioides (Hamilton) : a cell line susceptible to grouper nervous necrosis virus (GNNV). Journal of Fish Diseases 22, 173-182.
Chi, S.C., Lo, B.J., Lin, S.C., Wen, W.W., Lo, C.F., Kou, G.H., Chen, S.N., 2000. The survey of viral nervous necrosis among cultured groupers in Taiwan. In:APEC FWG02/2000. Bangkok, 18-20.
Chi, S.C., Lo, B.J., Lin, S.C., 2001. Characterization of grouper nervous necrosis virus (GNNV). Journal of Fish Diseases 24, 3-13.
Chi, S.C., Lo, C.F., Kou, G.H., Chang, P.S., Peng, S.E., Chen, S.N., 1997. Mass mortalities associated with viral nervous necrosis (VNN) disease in two species of hatchery-reared grouper, Epinephelus fuscogutatus and Epinephelus akaara (Temminck&Schlegel). Journal of Fish Diseases 20, 185-193.
Chi, S.C., Shieh, J.R., Lin, S.J., 2003. Genetic and antigenic analysis of betanodaviruses isolated from aquatic organisms in Taiwan. Diseases of Aquatic Organisms 55, 221-228.
Chong, S.Y., Ngoh, G.H., Chew-Lim, M., 1990. Study of three tissue culture viral isolates from marine foodfish. Singapore Journal of Primary Industry 18, 54-57.
Chua, F.H.C., Ng, M.L., Ng, K.L., Loo, J.J., Wee, J.Y., 1994. Investigation of outbreaks of a novel disease, 'Sleepy Grouper Disease', affecting the brown-spotted grouper, Epinephelus tauvina Forskal. Journal of Fish Diseases 17, 417-427.
Comps, M., Trindade, M. Delsert, Cl., 1996. Investigation of fish encephalitis viruses (FEV) expression in marine fishes using DIG-labelled probes. Aquaculture 143, 113-121.
Dalla Valle, L., Toffolo, V., Lamprecht, M., Maltese, C., Bovo, G., Belvedere, P., Colombo, L., 2005. Development of a sensitive and quantitative diagnostic assay for fish nervous necrosis virus based on two-target real-time PCR. Veterinary Microbiology 110, 167-179.
Dalla Valle, L., Zanella, L., Patarnello, P., Paolucci, L., Belvedere, P., Colombo, L., 2000. Development of a sensitive diagnostic assay for fish nervous necrosis virus based on RT-PCR plus nested PCR. Journal of Fish Diseases 23, 321-327.
Danayadol, Y., Krachaiwong, V., Ruangpan, L., Direkbusarakom, S., 1996. Causative agents and control measures of red boil disease in culture grouper (Epinephelus malabaricus). Thai Fish. Gaze. 49, 29-35.
Dijkstra, J.M., Fischer, U., Sawamoto, Y., Ototake, M., Nakanishi, T., 2001. Exogenous antigens and the stimulation of MHC class I restricted cell-mediated cytotoxicity: possible strategies for fish vaccines. Fish Fish & Shellfish Immunology 11, 437-458.
Evans, F.L., 1972. Ozone in Water and Wastewater Treatment. Ann Arbour Science Publishers Inc., Ann Arbor.
Fange, R., 1986. Lymphoid organs in sturgeons (Acipenseridae). Veterinary Immunology and Immunopathology 12, 153-161.
Fenner, B.J., Du, Q., Goh, W., Thiagarajan, R., Chua, H.K., Kwang, J., 2006a. Detection of betanodavirus in juvenile barramundi, Lates calcarifer (Bloch), by antigen capture ELISA. Journal of Fish Diseases 29, 423-432.
Fenner, B.J., Goh, W., Kwang, J., 2006b. Sequestration and protection of double-stranded RNA by the betanodavirus b2 protein. Journal of Virology 80, 6822-6833.
Fenner, B.J., Thiagarajan, R., Chua, H.K., Kwang, J., 2006c. Betanodavirus B2 is an RNA interference antagonist that facilitates intracellular viral RNA accumulation. Journal of Virology 80, 85-94.
Fishelson, L., 1995. Cytological and morphological ontogenesis and involution of the thymus in cichlid fishes (Cichlidae, Teleostei). Journal of Morphology 223, 175-190.
Frerichs, G.N., Morgan, D., Hart, D., Skerrow, C., Roberts, R.J., Onions, D.E., 1991. Spontaneously productive C-type retrovirus infection of fish cell lines. Journal of General Virology 72, 2537-2539.
Frerichs, G.N., Rodger, H.D., Peric, Z., 1996. Cell culture isolation of piscine neuropathy nodavirus from juvenile sea bass, Dicentrarchus labrax. Journal of General Virology 77, 2067-2071.
Fukuda, Y., Nguyen, H.D., Furuhashi, M., Nakai, T., 1996. Mass mortality of cultured sevenband grouper, Epinephelus septemfasciatus, associated with viral nervous necrosis. Fish Pathology 31, 165-170.
Fukuda, Y., Nguyem, H.D., Furuhashi, M., Nakai, T., 1999. Mass mortality of cultured seven band grouper, Epinephelus septemfasticatus, associated with viral nervous necrosis. Fish Pathology 31, 167-170.
Glazebrook, J.S., Heasman, M.P., Beer, S.W., 1990. Picorna-like viral particles associated with mass mortalities in larval barramundi, Lates calcarifer (Bloch). Journal of Fish Diseases 13, 245-249
Glazebrook, J.S., 1995. Disease risks associated with translocation of a virus lethal for barramundi (Lates calcarifer). Masters thesis, Griffith University, Brisbane, Queensland, Australia.
Gomez, D.K., Lim, D.J., Baeck, G.W., Youn, H.J., Shin, N.S., Youn, H.Y., Hwang, C.Y., Park, J.H., Park, S.C., 2006. Detection of betanodaviruses in apparently healthy aquarium fishes and invertebrates. Journal of Veterinary Science 7, 369-374.
Grotmol, S., Nerland, A.H., Biering, E., Totland, G.K., Nishizawa, T., 2000. Characterisation of the capsid protein gene from a nodavirus strain affecting the Atlantic halibut Hippoglossus hippoglossus and design of an optimal reversetranscriptase polymerase chain reaction (RT-PCR) detection assay. Diseases of Aquatic Organisms 39, 79-88.
Grotmol, S., Totland, G.K., Thorud, K., Hjeltnes, B.K., 1997. Vacuolating encephalopathy and retinopathy associated with a nodavirus-like agent: a probable cause of mass mortality of cultured larval and juvenile Atlantic halibut Hippoglossus hippoglossus. Diseases of Aquatic Organisms 29, 85-97.
Grotmol, S., Totland, G.K., 1999. Surface disinfection of Atlantic
halibut Hippoglossus hippoglossus eggs with ozonated sea-water
inactivates nodavirus and increases survival of the larvae. Diseases of Aquatic Organisms 39, 89-96.
Grotmol, S., Dahl-Paulsen, E., Totland, G.K., 2003. Hatchability of eggs from Atlantic cod, turbot and Atlantic halibut after disinfection with ozonated seawater. Aquaculture 221, 245-254.
Grove, S., Johansen, R., Reitan, L.J., Press, C.M., Dannevig, B.H., 2006. Quantitative investigation of antigen and immune response in nervous and lymphoid tissues of Atlantic halibut (Hippoglossus hippoglossus) challenged with nodavirus. Fish & Shellfish Immunology 21, 525-539.
Hegde, A., Chen, C.L. Qin, Q.W., Lam, T.J., Sin, Y.M., 2002. Characterization, pathogenicity and neutralization studies of a nervous necrosis virus isolated from grouper, Epinephelus tauvina, in Singapore. Aquaculture 213, 55-72.
Hegde, A., Teh, H.C., Lam, T.J., Sin, Y.M., 2003. Nodavirus infection in freshwater ornamental fish, guppy, Poicelia reticulata – comparative characterization and pathogenicity studies. Archives of virology 148, 575-586.
Huang, B., Tan, C., Chang, S.F., Munday, B., Mathew, J.A., Ngoh, G.H., Kwang, J., 2001. Detection of nodavirus in barramundi, Lates calcarifer (Bloch), using recombinant coat protein-based ELISA and RT–PCR. Journal of Fish Diseases 24, 135-142.
Husgaro, S., Grotmol, S., Hjeltnes, B.K., Rodseth, O.M., Biering, E., 2001. Immune response to a recombinant capsid protein of striped jack nervous necrosis virus (SJNNV) in turbot Scophthalmus maximus, and evaluation of a vaccine against SJNNV. Diseases of Aquatic Organisms 45, 33-44.
Ito, Y., Okinaka, Y., Mori, K.I., Sugaya, T., Nishioka, T., Oka, M., Nakai, T., 2008. Variable region of betanodavirus RNA2 is sufficient to determine host specificity. Diseases of Aquatic Organisms 79, 199-205.
Iwamoto, T., Mori, K., Arimoto, M., Nakai, T., 1999. High permissivity of the fish cell line SSN-1 for piscine nodaviruses. Diseases of Aquatic Organisms 39, 37-47.
Iwamoto, T., Nakai, T., Mori, K., Arimoto, M., Furusawa, I., 2000. Cloning of the fish cell line SSN-1 for piscine nodaviruses. Diseases of Aquatic Organisms 43, 81-89.
Johansen, R., Grove, S., Svendsen, A.K., Modahl, I., Dannevig, B., 2004. A sequential study of pathological findings in Atlantic halibut, Hippoglossus hippoglossus (L), throughout one year after an acute outbreak of viral encephalopathy and retinopathy. Journal of Fish Diseases 27, 327-341.
Knappskog, D.H., Sommer, A.I., Rodseth, O.M., 1999. Efficacy testing of different vaccine formulations using a laboratory IPNV challenge model (immersion). In: Proceedings of The European Association of Fish Pathologists,the International Conference, Diseases of Fish and Shellfish, Rhodes, Greece, 19-24 Sept.
Lai, Y.S., Chiu, H.C., Murali, S., Guo, I.C., Chen, S.C., Fang, K., Chang, C.Y., 2001. In vitro neutralization by monoclonal antibodies against yellow grouper nervous necrosis virus (YGNNV) and immunolocalization of virus infection in yellow grouper, Epinephelus awoara (Temminck & Schlegel). Journal of Fish Diseases 24, 237-244.
Lai, Y.S., Murali, S., Chiu, H.C., Ju, H.Y., Lin, Y.S., Chen, S.C., Guo, I.C., Fang, K., Chang, C.Y., 2001. Propagation of yellow grouper nervous necrosis virus (YGNNV) in a new nodavirus-susceptible cell line from yellow grouper, Epinephelus awoara (Temminck & Schlegel), brain tissue. Journal of Fish Diseases 24, 299-309.
Le Breton, A., Grisez, L., Sweetman, J., Ollevier, F., 1997. Viral nervous necrosis (VNN) associated with mass mortalities in cage-reared sea bass,Dicentrarchus labrax (L.). Journal of Fish Diseases 20, 145-151.
Lecocq-Xhonneux, F., Thiry, M., Dheur, I., Rossius, M., Vanderheijden, N., Martial, J., de Kinkelin, P., 1994. A recombinant viral haemorrhagic septicaemia virus glycoprotein expressed in insect cells induces protective immunity in rainbow trout. Journal of General Virology 75, 1579-1587.
Lee, K.K., 1995. Pathogenesis studies on Vibrio alginolyticus in the grouper, Epinephelus malabaricus , Bloch et Schneider. Microbial Pathogenesis 19, 39-48.
Leong, J.C., Fryer, J.L., 1993. Viral vaccines for aquaculture. Annual Review of Fish Diseases. 3, 225-240.
Lin, C.H., Christopher John, J.A., Lin, C.H., Chang, C.Y., 2006. Inhibition of nervous necrosis virus propagation by fish Mx proteins. Biochemical and Biophysical Research Communications 351, 534-539.
Lin, C.C., Lin, H.Y., Chen, M.S., Yang, H.L., 2007. An oral nervous necrosis virus vaccine that induces protective immunity in larvae of grouper (Epinephelus coioides). Aquaculture 268, 265-273.
Liu, W., Hsu, C.H., Chang, C.Y., Chen, H.H., Lin, C.S., 2006. Immune response against grouper nervous necrosis virus by vaccination of virus-like particles. Vaccine 24, 6282-6287.
Lorenzen, N., Lorenzen, E., Einer-Jensen, K., Heppell, J., Davis, H.L., 1999. Genetic vaccination of rainbow trout against viral haemorrhagic septicaemia virus: small amounts of plasmid DNA protect against a heterologous serotype. Virus Research 63, 19-25.
Lorenzen, N., Olesen, N.J., 1997. Immunization with viral haemorrhagic septicaemia antigens. In: Gudding, R., Lillehaug, A., Midtlying, P.J., Brown, F. (Eds.), Fish Vaccinology. Dev. Biol. Stand. 90 Karger, Basel, New York, pp. 201-209.
Magnadottir, B., 2006. Innate immunity of fish (overview). Fish & Shellfish Immunology 20, 137-151.
Maeno, Y., De La Pen�k�ka, L.D., Cruz-Lacierda, E.R., 2002. Mass mortalities associated with viral nervous necrosis in hatchery-reared orange-spotted grouper in the Philippines. Japan International Research Center for Agricultural Sciences 22.
Maeno, Y., De La Pen�k�ka, L.D., Cruz-Lacierda, E.R., 2004. Mass mortalities associated with viral nervous necrosis in hatchery-reared sea bass Lates calcarifer in the Philippines. JARQ 38, 69-73.
Mori, K., Nakai, T., Muroga, K., Arimoto, M., Mushiake, K., Furusawa, I., 1992. Properties of a new virus belonging to Nodaviridae found in larval striped jack (Pseudocaranx dentex) with nervous necrosis. Virology 187, 368-371.
Mori, K., Nakai, T., Nagahara, M., Muroga, K., Mekuchi, T., Kanno, T., 1991. A viral disease in hatchery-reared larvae and juveniles of redspotted grouper. Fish Pathology 26, 209-210.
Munday, B.L., Nakai, T., 1997. Special topic review: nodaviruses as pathogens in larval and juvenile marine finfish. World Journal of Microbiology and Biotechnology 13, 375-381.
Munday, B.L., Kwang J., Moody N., 2002. Betanodavirus infections of teleost fish: a review. Journal of Fish Diseases 25, 127-142.
Muroga, K., 1995. Viral and bacterial disease in larval and juvenile marine fish and shellfish: a review. Fish Pathology 30, 71-85
Mushiake, K., Arimoto, M., Furusawa, T., Furusawa, I., Nakai, T., Muroga, K., 1992. Detection of antibodies against striped jack nervous necrosis virus (SJNNV) from brood stocks of striped jack. Nippon Suisan Gakkaishi 58, 2351-2356.
Mushiake, K., Nishizawa, T., Nakai, T., Furusawa, I., Muroga, K., 1994. Control of VNN in striped jack: selection of spawners based on the detection of SJNNV gene by polymerase chain reaction (PCR). Fish Pathology 29, 177-182.
Nakai, T., Nguyen, H.D., Nishizawa, T., Muroga, K., Arimoto, M., Ootsuki, K., 1994. Occurrence of viral nervous necrosis in kelp grouper and tiger puffer. Fish Pathology 29, 211-214.
Nakai, T., Mori, K., Arimoto, M., Muroga, K., 1995. Neutralizing antibody production in striped jack immunized with a recombinant coat protein of striped jack nervous necrosis virus (SJNNV). In: Proceedings of The European Association of Fish Pathologists, Seventh International Conference, Diseases of Fish and Shell fish Palma de Mallorca, 10-15 Sept.
Nakajima, K., Ito, T., Kurita, J., Kawakami, H., Itano, T., Fukuda, Y., Aoi, Y., Tooriyama, T., Manabe, S., 2002. Effectiveness of a vaccine against red sea bream iridoviral disease in various cultured marine fish under laboratory conditions. Fish Pathology 37, 90-91.
Nakanishi, T., Fischer, U., Dijkstra, J.M., Hasegawa, S., Somamoto, T., Okamoto, N., Ototake, M., 2002. Cytotoxic T cell function in fish. Developmental and Comparative Immunology 26, 131-139.
Nash, G., Anderson, I.G., Shariff, M., Shamsudin, M.N., 1987. Bacteriosis associated with epizootic in the giant sea perch, Lates calcarifer, and the estuarine grouper, Epinephelus tauvina, cage cultured in Malaysia. Aquaculture 67, 105-111.
Navarro, V., Quesada, J.A., 1993. Immunochemical characterization of monoclonal antibodied to gilthead seabreami (Sparus aurate) immunoglobulin. Fish & Shellfish Immunology. 3, 167-177.
Navarro, V., Quesada, J.A., Abad, M.E., Taverne, N., Rombout, J.H.W.M., 1993. Immuno (cyto) chemical characterisation of monoclonal antibodies to gilthead seabream (Sparus aurata) immunoglobulin. Fish & Shellfish Immunology. 3, 167-177.
Nishizawa, T., Masaaki, K., Nakai, T., Muroga, K., 1995. Comparison of the coat protein genes of five fish nodaviruses, the causative agent of nervous necrosis in marine fish. Journal of General Virology 76, 1563-1569.
Nishizawa, T., Takano, R., Muroga, K., 1999. Mapping a neutralizing epitope on the coat protein of striped jack nervous necrosis virus. Journal of General Virology 80, 3023-3027.
Nguyen, H.D., Mekuchi, T., Imura, K., Nakai, T., Nishizawa, T., Muroga, K., 1994. Occurrence of viral nervous necrosis (VNN) in hatchery-reared juvenile Japanese flounder Paralichthys olivaceus. Fisheries Science 60, 551-554.
Oh, M.J., Jung, S.J., Kim, S. R., Rajendran, K.V., Kim, Y.J., Choi, T.J., Kim, H.R., Kim, J.D., 2002. A fish nodavirus associated with mass mortality in hatchery-reared red drum, Sciaenops ocellatus. Aquaculture 211, 1-7.
Okinaka, Y., Nakai, T., 2008. Comparisons among the complete genomes of four betanodavirus genotypes. Diseases of Aquatic Organisms 80, 113-121.
OIE (2000) Chapter 2.2.2. Viral encephalopathy and retinopathy.In: OIE Diagnostic Manual for Aquatic Animal Diseases, 3rd edn, pp. 69-73. OIE, Paris.
Patel, S., Korsnes, K., Bergh, O., Vik-Mo, F., Pedersen, J., Nerland A.H., 2007. Nodavirus in farmed Atlantic cod Gadus morhua in Norway. Diseases of Aquatic Organisms 77, 169-173.
Pilstrom, L., Petersson, A., 1991. Isolation and partial characterization of immunoglobulin from cod (Gadus morhua L.). Developmental and Comparative Immunology 15, 143-152.
Qin, Q.W., Wu, T.H., Jia, T.L., Hegde, A., Zhang, R.Q., 2006. Development and characterization of a new tropical marine fish cell line from grouper, Epinephelus coioides susceptible to iridovirus and nodavirus. Journal of Virological Methods 131, 58-64.
Roy, D., Wong, P.K.Y., Engelbrecht, R.S., Chian, S.K., 1981.
Mechanism of enteroviral inactivation by ozone. Microbiology
41, 718-723.
Scott, D.B.M., Lesher, E.C., 1963. Effect of ozone on survival and
permeability of Escherichia coli. Journal of Bacteriology 85, 567-576.
Secombes, C.J., Manning, M.J., 1980. Comparative studies on the immune system of fishes and amphibians: antigen localization in the carp Cyprinus carpio L. Journal of Fish Diseases. 3, 399-412.
Skliris, G.P., Richards, R.H., 1998. Assessment of the susceptibility of the brine shrimp Artemia salina and rotifer Brachionus plicatilis to experimental nodavirus infection. Aquaculture 169, 133-141.
Skliris, G.P., Richards, R.H., 1999. Nodavirus isolated from experimentally infected tilapia, Oreochromis mossambicus (Peters). Journal of Fish Diseases. 22, 315-318.
Sommerset, I., Nerland, A.H., 2004. Complete sequence of RNA1 and subgenomic RNA3 of Atlantic halibut nodavirus (AHNV). Diseases of Aquatic Organisms 58, 117-125.
Soria Moria Hotel,O., 1996. Fish Vaccinology, 50-65.
Starkey, W.G., Millar, R.M., Jenkins, M.E., Ireland, J.H., Muir, K.F., Richards, R.H., 2004. Detection of piscine nodaviruses by real-time nucleic acid sequence based amplification (NASBA). Diseases of Aquatic Organisms 59, 93-100.
Sweetman, E., Sweetman, J., Le Breton, A., Grisez, L., 1996. Nodavirus: a review of the findings of the XIV/Noda/95 investigation. Sea bass and seabream culture: problems and prospects. Handbook of Contributions
and Short Communications, Verona, Italy, October 16-18, 87-101.
Takizawa, N., Adachi, K., Ichinose, T., Kobayash, N., 2008. Efficient propagation of betanodavirus in a murine astrocytoma cell line. Virus Research 136, 206-210.
Tanaka, A., Aoki, H., Nakai, T., 1998. Pathogenicity of the nodavirus detected from diseased sevenband grouper Epinephelus septemfasciatus. Fish Pathology 33, 31-36.
Tanaka, S., Mori, K., Arimoto, M., Iwamoto, T., Nakai, T., 2001. Protective immunity of sevenband grouper, Epinephelus septemfasciatus Thunberg, against experimental viral nervous necrosis. Journal of Fish Diseases 24,15-22.
Thiery, R., Arnauld, C., Delsert, C., 1999. Two isolates of sea
bass, Dicentrarchus labrax L., nervous necrosis virus with distinct
genomes. Journal of Fish Diseases 22, 201-207.
Thomas Allnutt F.C., Bowers, R.M., Rowea, C.G., Vakharia, V.N., LaPatra, S.E., Dhar, A.K., 2007. Antigenicity of infectious pancreatic necrosis virus VP2 subviral particles expressed in yeast. Vaccine 25, 4880-4888.
Watanabe, K., Nishizawa, T., Yoshimizu, M., 2000. Selection of brood stock candidates of barfin flounder using an ELISA system with recombinant protein of barfin flounder nervous necrosis virus. Diseases of Aquatic Organisms 41, 219-223.
Wen, C.M., Lee, C.W., Wang, C.S., Cheng, Y.H., Huan, H.Y., 2008.
Development of two cell lines from Epinephelus coioides brain tissue for
characterization of betanodavirus and megalocytivirus infectivity and propagation. Aquaculture 278, 14-21.
Willett, C.E., Zapata, A.G., Hopkins, N., Steiner, L.A., 1997. Expression of zebrafish rag genes during early development identifies the thymus. Developmental Biology. 182, 331-341.
Wilson, M.R., Warr, G.W., 1992. Fish immunoglobulins and the genes that encode them. Annual Review of Fish Diseases 2, 201-221.
Yii, K.C., Yang, T.I., Lee, K.K., 1997. Isolation and characterization of Vibrio carchariae, a causative agent of gastroenteritis in the groupers, Epinephelus coicoides. Current Microbiology 35, 109-115.
Yoshikoshi, K., Inoue, K., 1990. Viral nervous necrosis in hatchery-reared larvae and juveniles of Japanese parrotfish, Oplegnathus fasciatus (Temminck & Schlegel). Journal of Fish Diseases 13, 69-77.
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