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研究生:陳世新
研究生(外文):Shih-Hsin Chen
論文名稱:飼料中不同動物性及植物性蛋白質比例對紫海膽(Anthocidariscrassispina)成長及生殖腺的影響
論文名稱(外文):The Effects of Different Animal and Plant Protein Ratio on the Growth and Gonad Somatic Index of Purple Sea Urchin (Anthocidaris crassispina)
指導教授:沈士新沈士新引用關係
指導教授(外文):Shyn-Shin Sheen
學位類別:碩士
校院名稱:國立臺灣海洋大學
系所名稱:水產養殖學系
學門:農業科學學門
學類:漁業學類
論文種類:學術論文
論文出版年:2008
畢業學年度:97
語文別:中文
論文頁數:74
中文關鍵詞:紫海膽蛋白質生殖腺
外文關鍵詞:Purple Sea UrchinProteinGonad
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本研究主要目的在探討飼料中不同蛋白質來源比例對成體紫海膽生殖腺指數及稚紫海膽成長之影響,及非生殖季節投餵人工飼料及水溫控制對成體紫海膽生殖腺之影響。
實驗一成體紫海膽初重為21.564±1.729 g,以大豆粉及魚粉為蛋白質來源,分別配置大豆粉蛋白質與魚粉蛋白質比例為0:4、1:3、1:1、3:1及4:0五組等蛋白(30%)等能量之實驗飼料,並以龍鬚菜(Gracilaria sp.)組為控制組。利用EAAI值評估飼料之胺基酸組成是否符合海膽胺基酸需求,結果以龍鬚菜組最高(0.924)而大豆粉蛋白質:魚粉蛋白質(4:0)最低(0.776)。經過90天的餵養實驗後,以增重率及生殖腺指數來評估其成長情況,結果顯示增重率以大豆粉蛋白質:魚粉蛋白質(1:3)最高,龍鬚菜組最低,生殖腺指數以大豆粉粉:魚粉(1:3)最高,龍鬚菜組最低。
實驗二稚紫海膽初重為0.103±0.001 g,以大豆粉及魚粉為蛋白質來源,分別配製大豆粉蛋白質與魚粉蛋白質比例為0:4、1:3、1:1、3:1及4:0五組等蛋白(30%)等能量之實驗飼料,並以龍鬚菜(Gracilaria sp.)組為控制組。利用EAAI值評估飼料之胺基酸組成是否符合海膽胺基酸需求,結果以龍鬚菜組最高(0.852)而魚粉蛋白質:大豆粉蛋白質(0:4)最低(0.699)。經過60天的餵養實驗後,以增重率及生殖腺指數來評估其成長情況,結果顯示增重率以魚粉蛋白質:大豆粉蛋白質(3:1)最高,龍鬚菜組最低,生殖腺指數以魚粉蛋白質:大豆粉蛋白質(3:1)最高,魚粉蛋白質:大豆粉蛋白質(0:4)最低。
實驗三以魚粉及大豆粉為蛋白質來源,配製成魚粉蛋白質:大豆粉蛋白質比例為3:1的實驗飼料,以龍鬚菜為對照組,分為加溫及不加溫兩組別,經過60天的餵養實驗後,以增重率及生殖線指數來評估飼料及水溫對海膽生殖腺的影響。結果顯示增重率在各處理組間無顯著差異,生殖腺指數以加溫並投餵人工飼料組最佳,不加溫投餵龍鬚菜組最差。
The purpose of this study was conducted to investigate the effects of different protein ratio and temperature control on somatic growth and gonad growth of adult and juvenile purple sea urchin (Anthocidaris crassispina). There are three experiments for this study. Gracilaria sp. Was used as a control diet.
Soybean meal and fish meal were used as the protein source with the five protein ratio (soybean meal:fish meal; 0:1、1:3、1:1 and 1:0) diets of experiment Ⅰ. EAAI value was used to estimate whether the amino acid composition of fulfills the need of adult purple sea urchin. The results showed that EAAI of Gracilaria sp. was the highest (0.924), and that of diet soybean meal:fish meal (4:0) was the lowest (0.776). The experiment duration was 90 days. The weight gain was high in sea urchin fed soybean meal:fish meal (1:3) diet and the low in sea urchin fed Gracilaria sp. The gonadal somatic index was high in sea urchin fed soybean meal:fish meal (1:3) diet and the low in sea urchin fed Gracilaria sp.
five protein ratio (soybean meal:fish meal; 0:1、1:3、1:1 and 1:0) diets of experiment Ⅱ. EAAI value was used to estimate whether the amino acid composition of fulfills the need of juvenile purple sea urchin. The results showed that EAAI of Gracilaria sp. was the highest (0.852) and that of diet soybean meal:fish meal (4:0) was the lowest (0.699). The experiment duration was 60 days. The weight gain was high in sea urchin fed soybean meal:fish meal (1:3) diet and the low in sea urchin fed soybean meal:fish meal (4:0) diet.
Soybean meal and fish meal were used as the protein source with the one protein ratio (Soybean meal:fish meal; 1:3) diet of experiment Ⅲ. The experimental duration was 60 days. The weight gain was high in sea urchin fed artificial diet with control water temperature and the low in sea urchin was fed Gracilaria sp. without control water temperature.
謝辭………………………………………………………………………Ⅰ
中文摘要…………………………………………………………………Ⅱ
英文摘要…………………………………………………………………Ⅲ
目錄………………………………………………………………………Ⅳ
表目錄……………………………………………………………………Ⅴ
圖目錄……………………………………………………………………Ⅵ

前言………………………………………………………………………1
文獻整理……………………………………………………………4
紫海膽之分類………………………………………………………4
紫海膽的生態習性…………………………………………………4
海膽生殖腺…………………………………………………………4
海膽生殖腺與產業關連性…………………………………………7
海膽的蛋白質需求…………………………………………………9
海膽的胺基酸需求…………………………………………………10
飼料之蛋白質來源…………………………………………………11
材料與方法………………………………………………………………14
實驗動物………………………………………………………………14
實驗系統………………………………………………………………14
實驗設計………………………………………………………………15
實驗飼料配製…………………………………………………………16
一般成分分析…………………………………………………………16
生殖腺指數……………………………………………………………16
胺基酸組成分析與必須胺基酸指數…………………………………16
統計分析………………………………………………………………18
結果………………………………………………………………………19
實驗一、飼料中不同動植物性蛋白質比例對成體紫海膽之影響…19實驗二、飼料中不同動植物性蛋白質比例對稚紫海膽之影響……20
實驗三、在非生殖季節下不同溫度對紫海膽之影響………………21
討論………………………………………………………………………23實驗一、飼料中不同動植物性蛋白質比例對成體紫海膽之影響…23實驗二、飼料中不同動植物性蛋白質比例對稚紫海膽之影響………………………………………25
實驗三、在非生殖季節下不同溫度對紫海膽之影響……………………26
飼料蛋白質對海膽體組成的影響…………………………………………26
海膽生殖腺的胺基酸組成…………………………………………………27
結論……………………………………………………………………………29
參考文獻………………………………………………………………………30
圖表……………………………………………………………………………38
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