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研究生:沈宣妤
研究生(外文):Hsuan-Yu Shen
論文名稱:烏魚分子標誌研究
論文名稱(外文):Studies of molecular markers of the grey mullet (Mugil cephalus)
指導教授:陳昭德陳昭德引用關係
指導教授(外文):Jau-Der Chen
學位類別:碩士
校院名稱:國立臺灣海洋大學
系所名稱:水產養殖學系
學門:農業科學學門
學類:漁業學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:111
中文關鍵詞:烏魚隨機擴增多型性DNA卵重體重比
外文關鍵詞:Mugil cephalusRAPDGSI
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烏魚為台灣重要的水產品之一。烏魚可分成四部份出售,烏魚體、烏魚卵、烏魚膘及烏魚肫,其中以烏魚子為烏魚最具有經濟價值的部分,人工養殖三年的烏魚,收成時烏魚生殖腺若越大,經濟價值越高。本研究擬應用隨機擴增多形性 (Random amplifies polymorphic DNA,RAPD)標誌,選取具高卵重體重比 (High GSI)特徵的烏魚做分子育種 (molecular breeding)用於篩選種魚,來確保所收成的烏魚子收益。
本研究由571尾的烏魚DNA樣本中篩選出 [A] p001G470之470 bp條帶、[B] p017G430之430 bp條帶、[F] p043(m46)G512之512 bp條帶、[O] p075G204之204 bp條帶、[S] p087G442之442 bp條帶、[T] p087G762之762 bp條帶、[Y] p102G374之374 bp條帶,此七條RAPD條帶及其Nested PCR結果,證明該七條基因座 (Allele)僅存在於GSI 17%以上的烏魚DNA樣本中,可用為篩選高卵重體重比的烏魚,做為種魚之參考。
The gery mullet, Mugil cephalus, is an important cultured fish species in Taiwan. The value of the grey mullet can divided into four parts : body, ovary, seminal vesicle,and stomach. The ovaries are the most valuable part.
RAPD markers were adopted to select the traits of high GSI of the grey mullet and would expect to be applied in molecule breeding a new generation to ensure the high yields of the ovaries.
The selected RAPD markers wered screened from 571 gery mullet DNA samples. We find the RAPD primer of [A] p001G470 with a DNA fragment at 470 bp, [B] p017G430 with a DNA fragment at 430 bp,[F] p043G512 with a DNA fragment at 512 bp, [O] p075G204 with a DNA fragment at 204 bp, [S] p087G442 with a DNA fragment at 442 bp,[T] p087G762 with a DNA fragment at 762 bp,and [Y] p102G374 with a DNA fragment at 374 bp.This seven RAPD fragments and the nested PCR result can prove the alleles existed in the genome of mullet and their GSI were more than 17%.
摘要..............................................I
Abstract..........................................III
目錄..............................................IV
圖目錄.............................................V
表目錄.............................................VI
前言...............................................1
一、烏魚簡介........................................1
二、核?酸指紋(DNA Fingerprinting)簡介...............3
三、DNA遺傳標記在養殖業上的應用.......................9
四、研究目的........................................10
材料與方法..........................................12
一、樣本採集........................................12
二、樣品基因組去氧核醣核酸(Genomic DNA)之製備.........12
三、第一階段烏魚樣本篩選...............................13
四、隨機擴增片段多型性(Random Amplified Polymorphic
DNA, RAPD)之分析.................................14
五、驗證第一階段篩選之烏魚分子標誌......................19
結果................................................22
一、第一階段篩選之RAPD分子標誌.........................22
二、含RAPD primer序列之烏魚DNA片段其轉殖定序結果........22
三、烏魚分子標誌篩選結果...............................24
討論.................................................27
RAPD引子的篩選........................................27
烏魚分子標誌篩選分析之討論..............................28
參考文獻..............................................31
表...................................................41
圖...................................................88
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