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研究生:連子揚
研究生(外文):Tzu-Yang Line
論文名稱:使用改良型尿液收集器於吳郭魚24小時維生素B6吸收之研究
論文名稱(外文):Studies on 24-hours the vitamin B6 absorption of hybrid tilapia(Oreochromis niloticus × O.aureus) by modified cannulated method
指導教授:黃沂訓黃沂訓引用關係
指導教授(外文):Yii-Shing Huang
學位類別:碩士
校院名稱:國立臺灣海洋大學
系所名稱:水產養殖學系
學門:農業科學學門
學類:漁業學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:72
中文關鍵詞:吳郭魚維生素B6
外文關鍵詞:Hydrid tilapiaB6
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本試驗以灌食及注射方式提供雜交種吳郭魚(Oreochromis niloticus × O. aureus )體內不同濃度的維生素B6,並以改良型導尿管收集尿液並觀察吳郭魚體內維生素B6血液及尿液24小時之變化,以瞭解B6在吳郭魚體內 24小時之代謝情形。
本實驗以平均體重900 ± 80吳郭魚克分別灌食體重0.3%或作靜脈注射以提供維生素B6,灌食組飼料B6濃度為0、5、10、20、40、80ppm而注射組濃度為0、0.1、0.3、0.5及1ppm劑量為5cc各三重複。在完成灌食或注射後之2、4、6、8、10、12、16、20、24小時採集各組之血液及尿液;並於第24小時採其肝臟及肌肉進行B6分析。維生素B6分析以高效能液相層析儀進行分析。
試驗結果發現吳郭魚在灌食後之第2-4小時血液及尿液中B6濃度皆有上升的趨勢,除了80ppm組血液及尿液B6濃度高峰為10小時外其餘均出現第在4-6小時之間。注射組在注射後第2小時血液B6濃度均到達高峰,尿液B6濃度在4-6小時達到高峰,血液與尿液B6最高濃度時間點注射組比灌食組平均快2-4小時。灌食各組肌肉B6濃度240-250ppb之間肝臟則介於2400-2410ppb之間,注射各組肌肉B6濃度範圍介於230-240ppb之間肝臟則在2440-2470ppb之間,組間平軍結果發現均無顯著差異。
本試驗吳郭魚在灌食濃度10ppm維生素B6飼料及注射0.3ppm濃度B6溶液後,其尿液對血液迴歸線之斜率均高於灌食組或注射組其他各處理組之斜率,因此這兩個濃度應是吳郭魚在灌食及注射兩個試驗中所得到的最佳B6利用濃度。吳郭魚吸收B6的速率雖然會隨著所給予B6濃度上升而上升,然而當所給予之濃度增加時並沒有發現尿液中所排泄之B6有成正比例上升的現象,顯示雜交種吳郭魚對維生素B6的排泄速率則有其極限。本試驗結果尿液中B6濃度大多數組別在24小時實驗結束時,均維持在相當高的濃度,因此若能有效降低緊迫而將實驗延長至48小時,將可獲得有關雜交吳郭魚在B6代謝過程更的多訊息。
The purpose of this study was to investigate the vitamin B6 24hr metabolism in hybrid tilapia the two input methods (forced feeding and injection) with a modified cannulated urine collector in order to monitor the changes of plasma and urine vitamin B6 concentration within after the intiation of certain amount of exogruous vitamin B6 supply lithor by oral administration or by injection a 24-hour duration .
There were three replicates each having 3 runs of individual fish at a time, every fish was randomly received either an injection of 5ml vitamin B6 solution at a certain concentration of 0, 0.1, 0.3, 0.5, 1 ppm, or a vitamin B6 containing diet at a certain level of 0, 5, 10, 20, 40, 80ppm, in concentration .
It was observed that plasma vitamin B6 concentration in the fish of the injection group were peaked 2-4 hours earlier than those fish in the oral administration group, however the urine B6 concentration umory the all treatments writhin each of the two groups of fish were all peaked at the 4-6th hour. There were no significant differences in the liver and muscle vitamin B6 concentration among the treatments either in the injection group or in the oral administration group of fish. The range was between 240-250ppb in the muscle, 2400-2410ppb in the liver for the oral administration group of fish, while that for the injection group was 230-240ppb in the muscle and 2440ppb in the liver.
The delay of the peak of the plasma and urine B6 concentration in the oral administration group revealed that vitamin B6 must have been absorped slowly in the digestive system at least four hours before transported to circulation system even given a high level of supply either by injection or oral administration.
This finding indicated that the vitamin B6 excrated rate did not increase in proportion to the iucreasing level vitamin B6 supply though the absolute amount suggested that there B6 excretion did by the level enzyme available rathes than substrate level B6.within the 24-hr duration of B6 excretion in hybrid tilapia was limited. The value of the regression line slope of the urine B6 concentration against plasma B6 concentration was observed higher in the fish with orally administrated 10ppm B6 diet than those fish orally received other concentrations of vitamin B6 diets, and higher in the fish injected a dose of 0.3ppm B6 solution compare with those fish injected different doses. It was concluded that the most suitable level in B6 utilization was 10ppm for oral administration and 0.3ppm for injection.
Adding an indication such as innulin in vitamin B6 solutions for a clearance test as well as extending the experimental duration from 24 hours were recommended to assure more detailed and correct data could be collected.
謝辭 i
摘要 iii
Abstract v
表目錄 xii
List of tables xiii
圖目錄 xv
List of figures xvi
附錄 xviii
List of appendixs xix
前言 1
文獻整理 2
2.1 維生素B6的簡介 2
2.1.1 維生素B6的發展史 2
2.1.2維生素B6的化學性質與特性 2
2.1.3維生素B6的吸收、運輸及代謝 4
2.1.4蛋白質對維生素B6需求量的影響 4
2.1.5維生素B6需求量 5
2.1.6維生素B6缺乏症 6
2.1.7維生素B6過量的毒性 8
2.1.8維生素B6的生理功能 8
2.1.8-1脫羧作用 8
2.1.8-2轉胺反應 9
2.1.8-3糖質新生作用 9
2.1.8-4免疫系統方面 9
2.1.8-5菸鹼酸的形成 10
2.1.8-6神經系統方面 10
2.1.8-7造血功能 10
2.1.8-8其他功能 11
2.1.9維生素B6測定方法 11
材料與方法 13
3.1 實驗動物 13
3.2 試驗環境 13
3.3 蓄養條件 13
3.4 實驗飼料配置 13
3.5導尿管製作 14
3.6試驗設計 14
3.6.1實驗目的 14
3.6.2試驗設計流程圖 15
3.6.3-1灌食維生素B6實驗 16
3.6.3-2灌食操作 16
3.6.3-3導尿管安裝 16
3.6.3-4抽血操作 16
3.6.3-5樣本採集 17
3.6.4-1注射維生素B6實驗 17
3.6.4-2注射操作 17
3.6.4-3導尿管安裝 17
3.6.4-4抽血操作 18
3.6.4-5樣本採集 18
3.7分析方法 18
3.7.1飼料粗成分分析 18
3.7.1-1水分 18
3.7.1-2 灰分 19
3.7.1-3.粗蛋白質 19
3.7.1-4. 粗脂質 20
3.7.1-5. 粗纖維 20
3.7.1-6.不含氮萃取物 21
3.8維生素B6 含量分析測定: 21
3.8.1飼料維生素B6含量之測定 22
3.8.2血液維生素B6含量之測定 22
3.8.3尿液維生素B6含量之測定 22
3.8.4肝臟維生素B6含量之測定 23
3.8.5肌肉中維生素B6 含量之測定 23
3.9尿液維生素B6排泄速率 23
3.10統計分析 23
結果 24
4.1飼料成分分析 24
4.2血液中維生素B6濃度 24
4.2.1灌食處理組 24
4.2.2注射處理組 24
4.3尿液中維生素B6濃度 25
4.3.1灌食處理組 25
4.3.2注射處理組 26
4.4尿量 26
4.4.1灌食處理組 26
4.4.2注射處理組 27
4.5尿液維生素B6排放量 27
4.5.1灌食處理組 27
4.5.2注射處理組 27
4.6肝臟中維生素B6濃度 27
4.6.1灌食處理組 27
4.6.2注射處理組 28
4.7肌肉維生素B6濃度 28
4.7.1灌食處理組 28
4.7.2注射處理組 28
4.8尿液維生素B6排泄速率 28
4.8.1灌食處理組 28
4.8.2注射處理組 29
4.9血液B6(mg)對應尿液B6(ug)24小時重量迴歸線圖 29
4.9.1灌食處理組 29
4.9.2注射處理組 29
討論 30
5.1血液及尿液中維生素B6濃度 30
5.1.1灌食處理組 30
5.1.2注射處理組 30
5.2灌食、注射血液和尿液中維生素B6高峰時間差比較 31
5.3改良導尿管對採集尿液量影響 31
5.4尿液24小時維生素B6排放總量 32
5.5肌肉及肝臟中維生素B6濃度 32
5.5.1灌食處理組 32
5.5.2注射處理組 33
5.6第24小時血液尿液維生素B6濃度比較 33
5.6.1灌食處理組 33
5.6.2注射處理組 33
5.7維生素B6排泄速率 33
5.8血液B6(mg)對應尿液B6(ug)24小時重量迴歸線圖 34
結論與建議 35
參考文獻 36
表 45
圖 57
附錄 65
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