臺灣博碩士論文加值系統

Part I
利用配位基原位水熱合成 (Hydrothermal in situ ligand syntheses)，以銀(Ag)、氰(CN)與疊氮化鈉(NaN3)混合物，合成具有羧酸(COOH)立體網絡結構的晶體：Ag2(μ3-Hbdc)0.5(μ4-Hbdc)0.5 (1)，(H2bdc = 1,4-benzenedicarboxylic acid)；所得之淡綠色晶體經由單晶X-光繞射分析得知為三斜晶系(Triclinic)，空間群為P-1，a = 3.7498(2) A，b = 10.6948(6) A，c = 18.7318(11) A，α = 88.038(2)°，β = 85.150(2)°，γ = 84.565(2)°。此聚合物是由雙核的銀原子和兩個對苯二甲酸配位基所組成，雙核的Ag原子單位是藉由分子內的Ag–O鍵結合，沿著晶格a軸形成一維鏈狀(chain)，並且具有類似階梯狀的結構。除此之外，每一個1D chain會進一步和其周圍的1D chain結合，透過另一個Ag–O鍵結形成二維的層狀(layer)結構。而在這個八元環(Ag2O4C2)聚合物chain的尾端有兩個氫鍵的存在。令人感興趣的是，2D layer彼此再藉由氫鍵的結合和配位基(Hbdc)上苯環間的π–π堆疊交互作用，形成一個三維(3D)網絡的結構。
在生物毒性的影響上，抗菌的實驗結果顯示化合物1大部分比硝酸銀(AgNO3)和對苯二甲酸(H2bdc)配位基表現出較佳的抑制效果。
Part II
整齊的中孔洞材料如MCM-41和SBA-15已經被成功的作為藥物遞送系統，它們具有可調整和一致性的孔洞尺寸、高表面積、大孔洞體積，因此可藉由中孔結構控制其藥物分子的釋放速率，然而，中孔洞材料具有較低生物活性，因為純的二氧化矽並不能直接進行骨組織的修補。近年來，中孔洞生物活性玻璃(MBGs)已經被成功的合成出來，這個新的生物活性材料家族，顯示出較好的骨形成生物活性，比起傳統的生物活性玻璃(BGs)，中孔洞生物活性玻璃有較好的生物活性。
本論文研究中孔洞生物活性玻璃的藥物釋放特性，並且選擇牛血清白蛋白(BSA)作為藥物分子模型的釋放研究。

Part I 目錄
謝誌 i
中文摘要 ii
英文摘要 iii
目錄 iv
表目錄 vi
圖目錄 vii
化合物一覽表 viii
第一章 緒論 1
1-1 前言 1
1-2 銀金屬配位聚合物 2
1-3 羧酸配位基 3
1-3-1 醋酸的藥物作用 4
1-4 銀對生物活性之影響 6
1-5 革蘭氏陽性與陰性菌之特性比較 7
1-6 研究動機 11
第二章 實驗部份 12
2-1 水熱合成與配位基原位水熱合成 12
2-1-1 水熱合成(Hydrothermal synthesis) 12
2-1-2 配位基原位水熱合成(Hydrothermal in situ ligand
synthesis) 14
2-2 儀器原理 15
2-2-1 單晶X-光繞射 15
2-2-2 晶體結構解析 18
2-2-3 X-光粉末繞射 18
2-3 實驗藥品與材料 18
2-3-1 配位基原位水熱合成 18
2-4 實驗儀器 19
2-4-1 配位基原位水熱合成 19
2-5 製備Ag2(μ3-Hbdc)0.5(μ4-Hbdc)0.5 19
2-6 抗菌試驗 20
2-6-1培養基製備 20
2-6-2菌株的保存 20
2-6-3菌株活化與菌種製備 21
2-6-4抗菌性質試驗 21
第三章 結果與討論 23
3-1 Ag2(μ3-Hbdc)0.5(μ4-Hbdc)0.5基本骨架剖析 23
3-2 Ag2(μ3-Hbdc)0.5(μ4-Hbdc)0.5之三維網絡結構 25
3-2-1 Ag原子配位形式 25
3-2-2 八元環系統 26
3-3 抗菌性質試驗 29
3-4 結論 33
參考文獻 34
Part II 目錄
中文摘要 i
英文摘要 ii
目錄 iii
表目錄 vi
圖目錄 vii
化合物一覽表 viii
第一章 緒論 1
1-1 前言 1
1-2 骨缺損修復材料 2
1-3 生物活性材料 3
1-3-1 生物活性定義 3
1-4 生物活性玻璃 3
1-4-1 生物活性玻璃簡介 3
1-4-2 生物活性玻璃合成方法 5
1-5 中孔洞材料 6
1-5-1 中孔洞材料的合成 7
1-5-2 中孔洞生物活性玻璃 9
1-6 藥物遞送 10
1-7 BSA簡介 12
1-8 研究動機 13
第二章 實驗部份 15
2-1 前言 15
2-2 實驗藥品與材料 15
2-3 實驗儀器 15
2-4 儀器原理 16
2-4-1 掃描式電子顯微鏡 16
2-4-2表面積暨孔洞分析儀 19
2-5 合成方法 24
2-5-1製備原理 24
2-5-2合成步驟 24
2-6 MBGs體外(in vitro)生物活性測試 25
2-7 牛血清白蛋白(BSA)吸附與釋放試驗 25
2-7-1載入方法 25
2-7-2 釋放方法 25
第三章 結果與討論 27
3-1 MBGs性質鑑定與討論 27
3-1-1 X光小角繞射 27
3-1-2 氮氣恆溫吸附脫附分析儀 28
3-1-3 29Si NMR固態圖譜 31
3-1-4 掃描式電子顯微鏡 31
3-2 牛血清白蛋白(BSA)之輸送測試 34
3-2-1 BSA載入試驗 34
3-2-2 BSA釋放試驗 35
3-3 結論 37
參考文獻 38

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