臺灣博碩士論文加值系統

在胚胎發育的過程中，細胞的分化與器官的發育、以及對環境刺激的反應與適應，是受到細胞內一系列嚴謹的訊息傳遞基因與調控系統所控制。其中basic-helix-loop-helix(bHLH)蛋白家族在生物體中參與了許多生理機制、荷爾蒙訊息傳導等。一般而言ARNT蛋白會和其他bHLH-PAS蛋白AHR、HIF和SIM結合形成異構體進而調控下游基因表現。過去的實驗發現，弱化arnt2基因會影響斑馬魚神經的發育及神經軸突的形成。
先前的研究發現arnt2基因的弱化會對神經膠細胞的發育造成影響，在此實驗中首先在利用兩種專一性morpholino(arnt2 MO2)弱化arnt2基因，藉以確認先前第一種arnt2 morpholino弱化對胚胎所造成的影響。結果證實無論以任一種專一性morpholino弱化arnt2，均會抑制少突神經膠細胞、星狀細胞、許旺細胞發育以及放射神經膠細胞等神經膠細胞的分化。同時也以組織切片證實arnt2弱化抑制mbp表現影響了髓鞘的變化。

During embryogenesis,the processes of cell differentiation, organogenesis and response to environmental stimulation are all controlled by various gene regulation system. The bHLH-PAS protein family play important roles in vertebrate development 、xenobiotic metabolism and hormone signal transduction. In the bHLH-PAS mediated pathway, ARNT can dimerize with AHR 、HIF and SIM to regulate variety gene. It was shown that repression of arnt2 gene caused severe defect in neuron development.
Previously,it was shown that blacking arnt2 translation resulted in sever defect of glial cell development. In here, we use two different arnt2 morpholino (MO2) to verify the function of arnt2 on glial cell development if appear that. the two new arnt2 morpholino blacked gilal cell development including olgodendrocyte、astrocyte、shwann cell and radil glial cell differention like previous arnt2 morpholino did. we have also confirned that arnt2 knockdown inhibited mbp expression and suppress myelin sheath formation.

摘要 i
Abstract ii
壹、前言 1
一、 bHLH-PAS蛋白與胚胎發育的關係 1
二、 神經和神經膠細胞的差異: 2
三、 神經膠細胞對於突觸的生長影響: 3
四、 神經膠細胞: 4
貳、實驗材料與方法 12
一. 實驗材料 12
二、實驗方法 18
?、結果 34
A.弱化arnt2基因表現對於少突神經膠細胞發育的影響 34
B.弱化arnt2基因表現對於星狀細胞發育的影響 35
C.弱化arnt2基因表現對於放射神經膠細胞發育的影響 35
D.弱化arnt2基因表現對於許旺細胞發育的影響 36
E.弱化arnt2基因表現對於髓鞘發育影響 36
肆、討論 38
弱化arnt2基因表現對於少突神經膠細胞發育影響: 38
弱化arnt2基因表現對於星狀細胞發育影響: 39
弱化arnt2基因表現對於放射神經膠細胞發育影響: 39
弱化arnt2基因表現對於許旺細胞發育影響: 40
弱化arnt2基因表現對於髓鞘發育影響: 40
伍、參考文獻 41
陸、圖表 45

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