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研究生:莊朝欽
論文名稱:和厚朴酚抑制巨噬細胞中細菌內毒素所誘發之細胞激素表現
論文名稱(外文):Honokiol Inhibits Lipopolysaccharide-induced Cytokines Expression in Macrophages
指導教授:洪良邦洪良邦引用關係趙國評趙國評引用關係
指導教授(外文):Lang-Bang Hung, Ph. D.Louis Kuoping Chao, Ph. D.
學位類別:碩士
校院名稱:國立臺灣海洋大學
系所名稱:食品科學系
學門:農業科學學門
學類:食品科學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:78
中文關鍵詞:和厚朴酚巨噬細胞細菌內毒素細胞激素
外文關鍵詞:HonokiolToll-like receptorLipopolysaccharideMacrophageCytokineProtein Kinase
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和厚朴酚 (Honokiol) 是一種由中藥材厚朴萃取出的酚類化合物,具有抗癌與抗發炎的效果。本論文探討和厚朴酚對細菌內毒素(LPS) 所引發細胞激素表現量的影響,發現和厚朴酚可以抑制LPS在巨噬細胞中所引發的腫瘤壞死因子�� �n(TNF-α) 與介白素1�n���n(IL-1β) 的表現量,並且可以抑制由LPS所引發的蛋白質酪胺酸磷酸化 (protein tyrosine phosphorylation)與絲裂原活化蛋白激?(mitogen-activated protein kinase)的磷酸化,包括 ERK1/2、JNK1/2 與p38 MAPK 。但和厚朴酚無法抑制 LPS 所引發的H2O2 產生。實驗結果顯示和厚朴酚透過抑制蛋白激脢活性,可降低細菌內毒素刺激後巨噬細胞所產生之細胞激素。
Honokiol, a phenolic compound originally isolated from Magnolia officinalis, shows anti-cancer and anti-inflammatory activities. However, until now, the effect of Honokiol on toll-like receptor 4 (TLR4)-mediated pro-inflammatory cytokines production is not clear. To elucidate the underlying mechanisms of the anti-inflammatory effect of Honokiol, macrophages stimulated with lipopolysaccharide (LPS) in the in the presence or absence of Honokiol. We observed that LPS-induced interleukin-1beta and tumor necrosis factor-alpha secretion from macrophages were reduced by Honokiol treatment in a dose-dependent fashion. Honokiol suppressed protein tyrosine phosphorylation as well as the phosphorylation level of mitogen-activated protein kinases, including ERK1/2, JNK1/2 and p38 in LPS-stimulated macrophages, suggesting a critical role of Honokiol in abrogating the protein kinase-mediated signaling; but unable to reduce reactive oxygen species release from LPS-stimulated macrophages. In summary, our current results demonstrate that Honokiol suppresses cytokines expression through the inhibition of protein kinases signaling, which provides a possible mechanism for its anti-inflammatory actions.
目錄
摘要............................Ⅰ
目錄............................Ⅲ
圖目錄..........................Ⅷ
一、前言.........................1
二、文獻整理......................4
(一)厚朴簡介....................4
(二)厚朴之運用..................6
1.厚朴於中藥之運用................6
2.厚朴於西藥上之運用..............7
(三)厚朴抗氧化機制之探討.........11
(四)厚朴抗發炎機制之探討.........13
三、實驗材料與方法
(一)實驗材料....................14
1.樣品...........................14
2.藥品...........................14
3.抗體...........................14
4.儀器設備........................15
(二)實驗方法.....................15
1.實驗設計備.......................15
(1)和厚朴酚抑制促發炎細胞激素(IL-1β)表現試驗..15
(2)和厚朴酚抑制促發炎細胞激素腫瘤壞死因子 (TNF-α) 表現試驗..16
(3)和厚朴酚在不同時間點抑制促發炎細胞激素的表現試驗..16
(4)和厚朴酚對腫瘤壞死因子表現的影響試驗..16
(5)和厚朴酚如何抑制細菌內毒素所誘發的白介質-1的前驅物pro-interleukin-1 (proIL-1)表現試驗..16
(6)和厚朴酚抑制細菌內毒素所誘發的人類 THP-1 單核球細胞腫瘤壞死因子試驗...17
(7)和厚朴酚抑制細菌內毒素所誘發的人類 THP-1 單核球細胞白介質-6試驗...17
(8)和厚朴酚抑制細菌內毒素所誘發的人類初代巨噬細胞促發炎細胞激素表現試驗...17
(9)和厚朴酚抑制細菌內毒素所誘發的人類初代巨噬細胞誘發的白介質-1的前驅物pro-interleukin-1 (proIL-1)表現試驗...18
(10)和厚朴酚抑制細菌內毒素所誘發的人類初代巨噬細胞誘發的腫瘤壞死因子表現試驗...18
(11)和厚朴酚抑制細菌內毒素所誘發之細胞激素的生成與活性氧族群的產生之關連試驗...18
(12) 和厚朴酚對絲裂原活化蛋白激脢活化的影響試驗...19
(13) 厚朴酚對細胞存活能力的影響試驗...19
(14) 厚朴酚對細胞增生能力的影響試驗....19
2.實驗方法....20
(1)人類初代巨噬細胞製備....20
(2)蛋白質之定量.....20
(3)西方點墨法.....21
(4)免疫酵素分析法.....22
(5)活性氧族群檢測.....23
(6)細胞增生檢測.....23
(7)統計分析量.....24
四、結果與討論.....25
(一)和厚朴酚對小鼠巨噬細胞中細胞激素之影響.....25
(二)和厚朴酚對人類巨噬細胞中細胞激素之影響.....28
(三)和厚朴酚對人類單核球細胞中細胞激素之影響.....30
(四)和厚朴酚對細菌內毒素所引起之活性氧族群之影響.....32
(五)和厚朴酚對細菌內毒素所活化之絲裂原活化蛋白激?之影響.....32
(六)和厚朴酚對細胞增生之影響.....34
五、結論.....36
六、參考文獻.....37
參考文獻
丁婉萍、唐星、陶秀梅。2003。厚朴提取物中主要成分的小腸吸收特性。瀋陽藥科大學學報,20(6):399-401。
王大力、孫豔濤、杜長青。2008。HPLC-MS厚朴有效成分研究。延邊大學學報,34(4):276-278。
王嘉、王鎪、王自強、陳菲、覃文新。2007。和厚朴酚抗血管生成作用的實驗研究。腫瘤,27(7):527-530。
王蓮華、蓋玉梅、袁成、曾林、賈暖。2004。厚朴酚與和厚朴酚藥代動力學的實驗研究。實用醫藥雜誌,21(2):137-139。
左克強、唐建華。2007。和厚朴酚及其抗腫瘤作用。岳陽職業技術學院學報,22(5):81-84。
孔苗、馮曉元、李文生、蔣士君。2005。和厚朴酚對水果採後致腐真菌的抑制作用。病理研究,3:10-12。
李杰萍、梁統、周克元。2002。厚朴酚對大鼠白細胞5-脂氧合?活性和細胞內鈣離子濃度的影響。廣東醫學院學報,20(3):177-178。
李杰萍、梁統、周克元。2003。厚朴酚對趨化三?激活的大鼠中性粒細胞功能的影響。中國醫藥大學學報,34(3):260-263。
林桂芸、謝生發、謝鴻、鷗俊。2003。和厚朴酚抑菌作用的研究。成都大學學報,22(2):18-20。
房志仲、楊金榮、李鑫、李璐、張慶偉、劉茂軍。2005。高效液相法測定解熱抗炎1號口服液中濃朴酚含量。The Journal of Pharmaceutic Practice,19︰2。
保志娟、楊雪瓊、鄒永明、苗兆濤、馬志剛、丁中濤。2005。厚朴酚與和厚朴酚清除DPPH•的作用。雲南大學學報,27(1)︰60-63。
孟超、吳豐、馬林。2008。厚朴類中藥厚朴酚及和厚朴酚含量測定。天然產物研究與開發,19:1024-102。
姜甯、劉曉鵬。2008。微波輔助提取厚朴中厚朴酚及和厚朴酚的研究。中華中醫藥雜誌,23(11):1012-1015。
袁成、賈暖、曾林、張莉、耿明、袁著忻。2003。厚朴提取物長期灌胃對小鼠腎臟的損害作用。藥學服務與研究,3(3):156-159。
徐詠斌、羅有福、陳俐娟。2009。高速逆流色譜分離純化合成的和厚朴酚抗腫瘤衍生物。四川化工,12(1):33-36。
郝慶紅、陳冠華、馮雅琪、郭雲霞。2008。和厚朴酚延緩小鼠衰老作用研究。河北農業大學學報,31(6):87-90。
郭文浩、曾霞。2006。和厚朴酚在抗腫瘤研究中的進展。華西醫學,21(1):179。
崔乘幸、許光日、湯波、許明錄。2008。中藥厚朴中厚朴酚、和厚朴酚的提取研究。河南科技學院學報,36(1):40-42。
陳端端。2007。不同炮製方法對厚朴中的厚朴酚與和厚朴酚的影響。醫學導刊,10:32-33。
許申鴻、杭瑚。1999。用DPPH•分析法研究野生植物的抗氧化活性。青島大學學報, 12(3):75-78。
曾志、沈妙婷、蒙紹金。2007。有機質譜學在蛇床子素、厚朴酚、和厚朴酚與魚腥草素結構確定中的應用。有機化學,27(1):92-96。
馮瑾、李繼遙、周學東。2007。厚朴活性成分對致齲菌生長和產酸影響的體外研究。四川大學學報,38(3):456-458。
楊光麗、侯文禮、付阿富、鐘振華、尹秀華、陳俐娟。2008。和厚朴酚對人宮頸癌細胞增殖和凋亡的影響。四川大學學報,39(4):558-562。
葉卉、冉曉輝、俞凱。2008。厚朴提取物工藝研究。醫藥導報,27(8):974-975。
雷曉溪、劉蘇、鄢秀菊、王強。2007。和厚朴酚抑制視網膜新生血管形成的實驗研究。中成藥,29(5):647-651。
劉可雲、董志、朱毅。2006。厚朴酚與和厚朴酚的藥理學研究現狀。中成藥,28(5):716-718。
劉麗、彭楓。2006。和厚朴酚的抗腫瘤作用研究進展。華西醫學,21(3):629-630。
穆紅,王玉亮,劉蓉。2006。野生型p53基因在八肝癌細胞的表達及誘導其凋亡。 細胞與分子免疫學雜誌, 22(6):758-759。
薛芳、李世輝、潘岐。2007。和厚朴酚對 U937 細胞的體外作用。中成藥,29(11):1582-1585。
蘇文娟、黃熙、秦峰、江莉、任平。2008。大鼠灌胃厚朴及厚朴三物湯後和厚朴酚的藥動學比較。中藥材,31(2):255-258。
顧偉、範昕建、吳疆、張莉敏、李曉芳、牛智強。2008。和厚朴酚對肝癌HepG2細胞生長抑制及凋亡的作用。細胞與分子免疫學雜誌,24(6):620-622。
Bai, X., Cerimele, F., Ushio-Fukai, M., Waqas, M., Campbell, P. M., Govindarajan, B., Der, C. J., Battle, T., Frank, D. A., Ye, K., Murad, E., Dubiel, W., Soff, G., and Arbiser, J. L. 2003. Honokiol, a small molecular weight natural product, inhibits angiogenesis in vitro and tumor growth in vivo. The Journal of Biological Chemistry. 278(37):35501-35507.
Battle, T., Arbiser, J., and Frank, D. 2005. The natural product honokiol induces caspase•-dependent apoptosis in B•-cell chronic lymphocytic leukemia(B-CLL)cells. Blood. 106(2): 690-697.
Burnette, W. N. 1981. “Western blotting” : electrophoretic transfer of proteins from sodium dodecyl sulfate — polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Analytical Biochemistry . 112 (2): 195–203.
Chao, L. K., Hua, K. F., Hsu, H. Y., Cheng, S. S., Lin, I. F., Chen, C. J., Chen, S. T., and Chang, S. T. 2008. Cinnamaldehyde inhibits pro-inflammatory cytokines expression via the reduction in levels of reactive oxygen species and mitogen-activated protein kinases within monocytes/macrophages. Food and Chemical Toxicology. 46: 220–231
Chen, F., Wang, T., Wu, Y. F., Gu, Y., Xu, X. L., Shu, Z., and Hu, X. 2004. Honokiol A potent chemotherapy candidate for human colorectal carcinoma. World Journal of Gastroenterology. 10(23): 3459-3463.
Chen, G., Xu, X., Zhu, Y., Zhang, L., and Yang, P. 2006. Determination of honokiol and magnolol in cortex Magnoliae Officinalis by capillary electrophoresis with electrochemical detection. Journal of Pharmaceutical and Biomedical Analysis. 41(4): 1479-1484.
Chiang, C.K., Sheu, M.L., Hung, K.Y., Wu, K.D., and Liu, S. H. 2006. Honokiol, a small molecular weight natural product, alleviates experimental mesangial proliferative glomerulonephritis. Kidney Int. 70(4): 682-689.
Chiu, J., Ho, C., Wei, Y., Lui, W., and Hong, C. 1997. In vitro and in vivo protective effect of honokiol on rat liver from peroxidative injury. Life Sciences. 61(19): 1961-1971.
Eikesdal, H. P., Bjerkvig, R., Dahl, O. 2001. Vinblastine and hyperthermia target the neovasculature in BT(4)AN rat gliomas: therapeutic implications of the vascular phenotype. International Journal of Radiation Oncology, Biology, Physics. 51(2):535-544.
Engvall, E., and Perlman, P. 1971. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry. 8 (9): 871–874.
Esuma, T., Makado, G., Zhai, H., Shimizu, Y., Mitsumoto, Y., and Fukuyama, Y. 2004. Efficient synthesis and structure activity relationship of honokiol, a neurotrophic biphenyl-type neolignan. Bioorganic & Medicinal Chemistry Letters. 14: 2621-2625.
Feng, J., Li, J. Y., and Zhou, X. D. 2007. Effects of the active compounds of M. officinalis on cariogenic bacteria. Journal of Sichuan University. 38(3): 456-458.
Fong, W., Tsa, A., Poon, K., and Wang C. 2005. Magnolol and honokiol enhance HL-60 human leukemia cell differentiation induced by 1,25-dihydroxyvitamin D3 and retinoic acid. The International Journal of Biochemistry & Cell Biology .37:427-441.
Fukuyama, Y., Nakade K., Minoshima Y., Yokoyama R., Zhai H., and Mitsumoto Y.2002. Neurotrophic activity of honokiol on the cultures of fetal rat cortical neurons. Bioorganic & Medicinal Chemistry Letters. 12:1163-1166.
Giuseppina, C., Luis, M., and Alessandra, B. 2003. Antioxidant chalcone glyomides and flavanones frcrn Maclura( Chlorophora) tinctoria. J. Nat. Prod. , 66: 1061-1064.
Hao, Q., Chen, G., Feng, Y., and Tian, Y. 2005. Determination of the main efficient composition in the herb of Magnolia officinalis for eliminating oxygen radicals. Journal of Hebei University(Natural Science Edition). 25(2): 164-168.
Harman, D. 1956. Aging: a theory based on free radical and radiation chemistry. Journal of Gerontology J Gerontol. 11(3):298-300.
Hibasami, H., Achiwa, Y., Katsuzaki, H., Imai, K., Yoshioka, K., Nakanishi, K., Ishii, Y., Hasegawa, M., and Komiya, T. 1998. Honokiol induces apoptosis in human lymphoid leukemia Molt 4B cells. International Journal of Molecular Medicine. 2(6):671-673.
Hirano, T., Gotoh, M., and Oka, K. 1994. Natural flavonoids and lignans are potent cytostatic agents against human leukemic HL-60 cells. Life sciences. 55(13):1061-1069.
Ho, K. Y., Tsai, C. C., Chen, C. P., Huang, J. S., and Lin, C. C. 2001. Antimicrobial activity of honokiol and magnolol isolated from Magnolia officinalis. Phytother Apy Research. 15(2):139-141.
Hsu Hsien-Yeh, Kuo-Feng Hua, Chun-Cheng Lin, Chun-Hung Lin, Jason Hsu, and Chi-Huey Wong. 2004. Extract of Reishi polysaccharides induces cytokine expression via TLR4-modulated Protein kinase signaling pathways. The Journal of Immunology. 173:5989-5999.
Hsu HY, Hua KF, Su YC, Chiu HW, Yang SS, Chao LK. 2006. Study on regulation of cytokine gene expression in macrophages with an alkali-soluble polysaccharide of Rhizoclonium riparium alga. Journal of Agricultural and Food Chemistry 54: 3558-3565.
Hu, H., Zhang, X. X., Wang, Y. Y., and Chen, S. Z. 2005. Honokiol inhibits arterial thrombosis through endothelial cell protection and stimulation of prostacyclin. Acta Pharmacologica Sinica. 26(9):1063-1068.
Huang, D., Yu Z., and Li, Q. 2005. Effects of honokiol and magnolol on beta- endorphin in relieving morphine withdrawal symptoms in rats. Chinese Journal of Clinical Rehabilitation. 9(32): 230-231.
Hua, K. F., Hsu, H. Y., Chao, L. K., Chen, S. T., Yang, W. B., Hsu, J., and Wong, C. H. 2007. Ganoderma lucidum Polysaccharides Enhance CD14 Endocytosis of LPS and promote TLR4 signal transduction of cytokine cxpression. Journal of Cellular Physiology. 212(2):537-550.
Hua, K. F., Hsu, H. Y., Su, Y. C., Lin, I. F., Yang, S. S., Chen, Y. M., and Chao, L. K. 2006. Study on the anti-inflammatory activity of methanol extract from seagrass Zostera japonica. Journal of Agricultural and Food Chemistry. 54(2):306-311.
Ikarashi, Y., Yuzurihara, M., Sakakibara, I., Nakai, Y., Hattori, N., and Maruyama, Y. 2001. Effects of the extract of the bark of Magnolia obovata and its biphenolic constituents magnolol and honokiol on histamine release from peritoneal mast cells in rats. Planta Medica. 67:709-713.
Irie, T., Miyamoto, E., Kitagawa, K., Maruyama, Y., Inoue, K., and Inagaki, C. 2001. An anxiolytic agent, dihydrohonokiol-B, inhibits ammonia-induced increases in the intracellular Cl‾ of cultured rat hippocampal neurons via GABAc receptors. Neuroscience Letters. 312:121-123.
Ishitsuka, K., Hideshima, T., Hamasaki, M., Raje, N., Kumar, S., Hideshima, H., Shiraishi, N., Yasui, H., Rocearo, A., Richardson, P., Podar, K., Gouill, S. L., Chauhan, D., Tamura, K., Arbiser, J., and Anderson, K. C. 2005. Honokiol overcomes conventional drug resis-tance in human multiple myeloma by induction of caspase-dependent and independent apoptosis. Blood. 106(5):1794-1800.
Ko, C.H., Chen , H.H., Lin, Y.R., and Chan, M.H. 2003. Inhibition of smooth muscle contraction by magnolol and honokiol in porcine trachea. Planta Medica. 69(6): 532-536.
Kong, L., Cheng, C., Tan, R. 2004.Inhibition of MAO A and B by some plant-derived alkaloids, phenols and anthraquinones. Journal of Ethnopharmacology. 91: 351-355.
Kong, M., Feng, X. Y., Li, W. S., and Jiang, S. J. 2005. Inhibitory Action of honokiol on rot-causing fungi in postharvest fruit. Storage &Process. 5(3): 10-13.
Kuribara, H., Stavinoha, W.B., and Maruyama, Y. 1999. Honokiol, a putative anxiolytic agent extracted from magnolia bark, has no diazepam-like side-effects in mice. Journal of Pharmacy and Pharmacology. 51(1):97-103.
Kuo, T., Yuh. C., and Cheng, M. 2003. The anti-inflammatory effect of honokiol on neutrophils: mechanisms in the inhibition of reactive oxygen species production. European Journal of Pharmacology. 475:19-27.
Lee, Y., Huang, C, Wen, K., and Suen T. 1995. Determination of liquiritin, glycyrrhizin, hesperidin, cinnamic acid, cinnamaldehyde, honokiol and mognolol in the traditional Chinese medicinal preparation Wuu-Ji-San by high performance liquid chromatography. Journal of Chromatography. 692:137-145.
Lee, B., Kim, C.H., and Moon, S. K. 2006. Honokiol causes the p21WAF1-mediated G(1)-phase arrest of the cell cycle through inducing p38 mitogen activated protein kinase in vascular smooth muscle cells. FEBS Letters. 5802(2): 5177-5184.
Lee, J., Jung, E., Park, J., Jung, K., Lee, S., Hong, S., Park, J., Park, E., Kim, J., Park, S., and Park, D. 2005. Anti-inflammatory effects of magnolol and honokiol are mediated through inhibition of the downstream pathway of MEKK-1 in NF-kappaB activation signaling. Planta Med. 71:338-343.
Lin, Y., Chen, H., Ko, C., and Chan, M. 2005. Differential inhibitory effects of honokiol and magnolol on excitatory amino acid-evoked cation signals and NMDA-induced seizures. Neuropharmacology. 49: 542-550.
Liou, K., Shen, Y., Chen, C., Tsao, C. and Tsai, S. 2003. Honokiol protects rat brain from focal cerebral ischemia reperfusion injury by inhibiting neutrophil infiltration and reactive oxygen species production. Brain Research. 992: 159-166.
Lin, Y. R., Chen, H. H., Ko, C. H., Chan, M. H. 2006. Neuroprotective activity of honokiol and magnolol in cerebellar granule cell damage. European Journal of Pharmacology. 537: 64-9.
Lin, Y. R., Chen, H. H., Ko, C. H., and Chan, M. H. 2005. Differential inhibitory effects of honokiol and magnolol on excitatory amino acid-evoked cation signals and NMDA-induced seizures. Neuropharmacology. 49: 542-50.
Liou, K. T., Shen, Y. C., Chen, C. F., Tsao, C. M., and Tsai, S. K. 2003. Honokiol protects rat brain from focal cerebral ischemia-reperfusion injury by inhibiting neutrophil infiltration and reactive oxygen species production. Brain Research. 992:159-66.
Lioua, K., Shen, Y., Chen, C., Tsao, C., and Tsai, S. K. 2003. The anti-inflammatory effect of honokiol on neutrophils: mechanisms in the inhibition of reactive oxygen species production. European Journal of Pharmacology. 475 :19-27.
Liou, K. T., Shen, Y. C., Chen, C. F., Tsao, C. M., and Tsai, S.K. 2003. The anti-inflammatory effect of honokiol on neutrophils: mechanisms in the inhibition of reactive oxygen species production. European Journal Pharmacology. 475:19-27.
Liou, K. T., Lin, S. M., Huang, S. S., Chih, C. L., and Tsai, S.K. 2003. Honokiol ameliorates cerebral infarction from ischemia-reperfusion injury in rats. Planta Medica. 69(2):130-134.
Liu, B., Hattori, N., Zhang, N.Y., ,Wu B., Yang, L., Kitagawa, K., Xiong, Z. M., Irie, T., and Inagaki, C. 2005. Anxiolytic agent, dihydrohonokiol-B, recovers amyloid beta protein-induced neurotoxicity in cultured rat hippocampal neurons. Neurosci Lett. 384: 44-47.
Martinez, A. L., Dominguez, F., Orozco, S., Chavez, M., Salgado, H., Gonzalez, M., Gonzalez-Trujano, M. E. 2006. Neuropharmacological effects of an ethanol extract of the Magnolia dealbata Zucc. leaves in mice. Journal of Ethnopharmacology. 106(2): 250-255.
Mosmann, T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological methods. 65 (1-2): 55–63.
Nagase, H., Ikeda, K., and Sakai, Y. 2001. Inhibitory effect of magnolol and honokiol from Magnolia obovata on human fibrosarcoma HT-1080. invasiveness in vitro. Planta medica. 67(8):705-708.
Nguyen, J., and Wells, J. 2003. Direct activation of the apoptosis machinery as a mechanism to target cancer cells. PNAS. 100(13):7533-7538.
Park, J., Lee, J., Jung, E., Park, Y., Kim, K., Park, B., Jung, K., Park, E., Kim, J., and Park, D.2004. In vitro antibacterial and anti-inflammatory effects of honokiol and magnolol against Propionibacterium sp.. European Journal Pharmacology. 496:189-95.
Pyo, M. K., Lee, Y. Y., Yun-Choi H. S. 2002. Anti-platelet effect of the constituents isolated from the barks and fruits of Magnolia obovata. Archives of Pharmacal Research. 25(3):325-328.
Sheu, S, and Lu, C. 1995. Determination of eight constituents of Hsiao-Cheng-chi-tang by high-performance liquid chromatography. Journal of Chromatography. 704:518-523.
Stoscheck, C. 1990. Quantification of Protein. Methods in Enzymology. 182:50-68.
Sungnoon, R., and Chattipakorn, N. 2005. Anti-arrhythmic effects of herbal medicine. Indian Heart Journal. 57(2):109-113.
Syu, W. J., Shen, C. C., Lu, J. J., Lee, G. H., and Sun, C.M. 2004. Antimicrobial and cytotoxic activities of neolignans from Magnolia officinalis. Chemistry Biodiversity. 1:530-7.
Tachikawa, E., Takahashi, M., and Kashimoto, T. 2000. Effects of extract and ingredients isolated from magnolia obovata thunberg on catecholamine secretion from bovine adrenal chromaffin Cells. Biochemical Pharmacology. 60: 433-440.
Tse, A., Wan, C., Shen, X., Yang, M., and Fong, W. 2005. Honokiol inhibits TNF-α-stimulated NF-κB activation and NF-κB-regulated gene expression through suppression of IKK activation. Biochemical Pharmacology. 70: 1443-1457.
Tsai, T., Chou, C., Cheng, F., and Chen, C. 1994. Pharmacokinetics of honokiol after intravenous administration in rats assessed using high-performance liquid chromatography.Journal of Chromatography. B, Biomedical applications. 655(2): 1-45.
Wang, T., Chen, F., Chen, Z., Wu, Y. F., Xu, X. L., Zheng, S., and Hu, X. 2004. Honokiol induces apoptosis through p53-independent pathway in human colorectal cell line RKO. World Journal of Gastroenterology. 10(15):2205-2208.
Wang, X., Wang, Y., Geng, Y., Li, f., and Zheng, C. 2004. Isolation and purification of honokiol and magnolol from cortex magnoliae officinalis by high-speed counter-current chromatography. Journal of Chromatography. 1036: 171-175.
Wu, X., Chen, X., and H, Z. 2003.High-performance liquid chromatographic method for simultaneous determination of honokiol and magnolol in rat plasma. Talanta. 59: 115-121.
Xu, D., Lu, Q., and Hu, X. 2006. Down-regulation of P-glycoprotein expression in MDR breast cancer cell MCF-7/ADR by honokiol. Cancer Lett. 243: 274-280.
Xue, F., Li, S. H., and Pan, L. 2007. External effect of honokiol on U937 cell. Chinese Traditional Patent Medicin. 29(11): 1582-1585.
Yamazaki, R., Sugatani, J., Fujii, I., Kuroyanagi, M., Umehara, K., Ueno A., Suzuki, Y., and Miwa, M. 1994. Development of a novel method for determination of acetyl-CoA:1-alkyl-sn-glycero-3-phosphocholine acetyltransferase activity and its application to screening for acetyltransferase inhibitors. Inhibition by magnolol and honokiol from Magnoliae cortex. Biochemical Pharmacology. 47: 995-1006.
Yang, S. E., Hsieh, M. T., Tsai, T. H., and Hsu, S. L. 2002. Down-modulation of Bacl-XL, release of cytochrome c and sequential activation of caspases during honokiol-induced apoptosis inhuman squamous lung cancer CH27 cells. Biochemical Pharmaeology. 63(9): 1641-1651.
Yuan, C., Jia, N., Zeng, L., Zhang, L., Geng, M., and Yuan, Z. X. 2003. Impairment of kidney induced by methanol extract of Magnolia officinalis after single or chronic administration in mice. Pharmacentical Care & Research. 3(3): 156-159.
Yuh, C., Yen, J., and Chieh F. 1998. Magnolol inhibits Mac-1(CD l1b/CD18)-dependent neutrophil adhesion: Relationship with its antioxidant effect. European Journal of Pharmacology. 343:79- 86.
Zhai, H., Nakade, K, Oda, M., Mitsumoto, Y., Akagi, M., Sakurai, J., and Fukuyama Y.2005. Honokiol-induced neurite outgrowth promotion depends on activation of extracellular signal-regulated kinases (ERK1/2). European Journal of Pharmacology .516: 112-117.
Zhang, X., Chen, S., and Wang, Y. 2007. Honokiol up-regulates prostacyclin synthease protein expression and inhibits endothelial cell apoptosis. European Journal Pharmacology. 554(1):1-7.
Zhang, W. W., Li, Y., Wang, X. Q., Tian, F., Cao, H., Wang, M. W., and Sun, Q. S. 2005. Effects of magnolol and honokiol derived from traditional Chinese herbal remedies on gastrointestinal movement. World Journal Gastroenterol. 11: 4414-4418.
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